Slc4a7 (NM_001270860) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	NBC3; NBCn1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217560 representing NM_001270860 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCAGACGGGGCCGGCGAGCAGATGAGACCGCTACTCACGCGGGGTCCCGATGAAGAAGCTGTTG TGGATCTTGGCAAAACTAGCTCAACCGTGAACACCAAGTTTGAAAAAGAAGAATTAGAGAGTCATCGAGC TGTATATGTTGGTGTTCATGTACCGTTTAGTAAAGAGAGTCGTCGGCGTCACAGGCATCGAGGGCACAAA CATCACCACCGGAGAAGAAAAGACAAAGACTCAGATAAGGAAGATGGACGGGAGTCTCCTTCTTATGACA CGCCATCGCAGAGGGTGCAGTTCATCCTTGGGACTGAAGACGATGATGAGGAGCACATTCCCCACGACCT CTTCACGGAGATGGACGAGCTCTGCTACCGAGACGGGGAAGAGTACGAGTGGAAAGAGACAGCCAGGTGG CTGAAGTTCGAAGAGGATGTTGAGGATGGTGGTGACCGATGGAGTAAACCGTATGTGGCCACTCTGTCTT TGCACAGTCTCTTTGAGCTGAGGAGTTGTATCCTAAATGGAACAGTCATGCTGGATATGAGAGCAAGCAC TCTGGATGAAATAGCAGATATGGTGTTAGACAACATGATAGCGTCTGGCCAGCTAGATGACTCCATAAGG GAGAATGTTCGAGAAGCTCTTCTGAAGAGACATCATCATCAAAATGAGAAAAGGTTTACAAGTCGGATTC CCCTCGTTCGATCCTTTGCAGATATAGGGGAAGGCCTGTCAGCCTCCCGCCATTCTTTGCGAACAGGTCT GTCTGCCTCAAACCTTTCCTTGAGAGGAGAATCGCCTTTATCCCTTCTTCTCAGTCATCTTCTTCCTTCT TCAAGAGCTGGCACCCCTGCAGGCTCAAGGTGTACCACCCCAGTACCCACCCCCCAGAACAGTCCTCCTT CCAGCCCCAGCTTAAGTCGCCTGACCTCCAGAAGTTCCCAACAGACTCAGCCTCAGGCCCCAGAAGTACT GGTGTCACCTGACAGGGATGATATTCCCAGAGTAGTAATTCATCCGCCTGAGGAAGACATAGAAGCACTG AAAGGCCAAGAGCAGAAGAATGAGGAAAATACTGACTTCACTCCAGGGATTTTGGCTTCTCCACAGTCTG CTCCTGGAAACCTGGACAGTAGTAAAAGTGGTGAAGTGAAAGGTAACGGAAGTGGAGGAAGCAGAGAAAA TAGTACTGTTGACTTTAGCAAGGTTGACATGAATTTCATGAGGAAAATTCCTACAGGAGCTGAGGCATCC AATGTTTTGGTAGGAGAGGTGGATTTCTTGGAGAGACCTATCATTGCATTTGTGAGACTGGCTCCTGCAG TTCTCCTCTCAGGGTTGACTGAGGTCCCTGTGCCCACTAGGTTTTTGTTTCTGTTACTGGGCCCAGCAGG AAAGGCTCCACAGTACCATGAAATTGGCAGATCCATAGCAACTCTAATGACAGATGAGATTTTTCATGAT GTAGCTTATAAAGCAAAAGATCGAAATGACCTCTTATCTGGAATTGATGAATTTTTAGATCAAGTAACTG TTCTTCCTCCAGGAGAGTGGGATCCTTCCATACGCATAGAGCCACCAAAAAGTGTTCCTTCTCAGGAGAA AAGGAAGATTCCTGCGTTTCCCAATGGATCTGCTCCAGTGTCTGCTGACCCTCCTAAGGAGGCTGATCAC CACGCTGGGCCTGAGCTGCAGAGGACTGGACGGCTTTTTGGTGGTTTGATACTTGACATCAAAAGGAAAG CACCTTTTTTCTTGAGTGACTTCAAGGATGCATTAAGTCTGCAGTGCCTGGCCTCGATTCTTTTCCTATA CTGTGCCTGTATGTCTCCTGTAATCACTTTTGGAGGGCTGCTTGGAGAAGCTACAGAAGGCAGAATAAGT GCAATAGAGTCTCTTTTTGGAGCATCATTAACTGGGATTGCCTATTCATTGTTTGCTGGGCAACCTCTAA CAATACTGGGGAGCACGGGTCCAGTCCTAGTGTTTGAAAAAATTTTATTTAAATTCTGTAGAGATTATCA CCTATCCTATCTATCATTAAGAACCAGTATTGGTCTGTGGACTTCTTTCTTGTGCATTGTGTTGGTCGCA ACAGATGCCAGCAGCCTTGTTTGTTACATTACTCGGTTCACAGAAGAAGCTTTCGCCGCACTCATTTGTA TCATCTTCATCTATGAAGCCTTGGAGAAACTCTTTCACTTAGGAGAAATATATGCATTTAACATGCACAA CAACTTGGATGCATTGACCAGTTACACATGTGTATGTGCTGAGCCATCTAATCCTAGCAACGAAACTGTA GAGCTGTGGGAGAGGAAGAACGTGACAGCAGCCAGTATTTCCTGGGCAAACCTTACCGTGTCTGAGTGTA AGACCTTCCACGGTATGTTTGTGGGATCAGCTTGTGGGCCTCACGGGCCTTATGTTCCCGATGTGCTCTT CTGGTGTGTCGTCTTGTTTTTCACAACGTTCTTTCTGTCTTCATTCCTCAAGCAGTTTAAGACCAAGAGA TATTTTCCTACTAAGGTGCGATCAACAATCAGTGACTTTGCTGTATTTCTCACAATAGTAATAATGGTTG CAATTGACTACCTTGTAGGAATTCCATCTCCTAAACTTCATGTTCCTGAAAAGTTTGAGCCTACTGATCC AAGCAGGGGCTGGATCATAAGCCCTTTGGGAGATAACCCTTGGTGGACCTTACTAATTGCAGCTGTTCCA GCTCTCCTTTGTACCATTCTCATCTTCATGGACCAGCAGATCACAGCTGTGATCATCAACAGGAAAGAAC ACAAACTGAAGAAAGGAGCTGGCTATCACCTGGACTTGCTCATGGTTGCTGTCATGTTGGGAGTCTGCTC CATCATGGGCCTGCCGTGGTTTGTGGCTGCAACAGTGTTGTCTATAAGTCATGTCAACAGCTTAAAAGTA GAGTCTGAATGTTCTGCTCCAGGGGAACAGCCCAAGTTCCTCGGAATTCGTGAACAGCGGGTTACAGGGC TGATGATTTTTATCCTGATGGGCCTCTCTGTGTTCATGACTTCAGTATTAAAGTTTATTCCGATGCCAGT TCTATATGGTGTTTTCCTTTATATGGGAGTTTCTTCTCTGAAAGGAATTCAGTTTTTTGACCGTATCAAA TTATTTGGAATGCCTGCCAAGCACCAGCCGGACCTGATCTACCTCCGCTATGTCCCTCTCTGGAAGGTGC ACGTGTTCACGGTCGTCCAGCTGACCTGCCTGGTTCTGCTCTGGGTGATCAAAGCCTCTGCTGCTGCAGT AGTTTTTCCCATGATGGTTCTTGCATTAGTCTTTGTGCGCAAGCTCATGGATCTGTGCTTCACAAAGAGA GAACTCAGTTGGCTTGATGACCTCATGCCAGAAAGTAAGAAAAAGAAAGAAGATGACAAGAAGAAGAAGG AGAAGGAGGAAGCTGAGCGGATGCTTCAGGGTGACGGGGATACTGTGCACCTCCCATTCGAAAGGGGGAG TCTCCTACAAATTCCAGTTAAGACCCTAAAATATAGTATTGACCCTTCAGTTGTTAACATATCAGATGAA ATGGCCAAAACTGCCCAGTGGAAGGCACTTTCCATGAATACTGAGAATGCCAAAGTAACCAGACCTAACA TGAGCCCTGAAAAGCCTGTGAGTGTGACAATAAATTTCGAAGATGAACCATCAAAAAAATACATGGATGC TGAAACTTCATTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270860
Insert Size	3726 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270860.1, NP_001257789.1
RefSeq Size	4002 bp
RefSeq ORF	3726 bp
Locus ID	117955
Gene Summary	regulates intracellular pH in different cells along with Na+-driven HCO3- transporters, the Na+-driven Cl-/HCO3- exchanger [RGD, Feb 2006] Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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