Trappc10 (NM_001173528) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Tmem1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217567 representing NM_001173528 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCCCCTGAGGAGCCACAGCCACCGGTGGTCTACACCATGGAGAACAAGCCTATCGTCACCTGTG CTGGAGACCAGAATTTATTTACCTCCATCTATCCGACACTTTCCCAGCAGCTTCCAAGAGAACCAATGGA GTGGAGAAGGTCCTATGGCCGGGCTCCGAAAATGATTCACCTGGAATCCAACTTTGTTCAGTTCAAGGAG GAGCTGCTGCCTAAGGAAGGGAACAAAGCCCTGCTCACCTTCCCCTTCCTCCACATCTACTGGACGGAGT GCTGTGACACTGAAGTATACAAAGCTACGGTAAAAGACGACCTCACCAAGTGGCAGAATGTTCTCAAAGC TCACAGCTCTGTGGATTGGCTGATAGTGGTAGTAGAAAATGATGCCAAGAAAAAAAACAAAACCAACATC CTTCCCAGAACTTCTATTGTGGACAAAATAAGAAATGACTTTTGTAATAAACAGAGTGACAGGTGTGTTG TGCTCTCTGACCCCTTGAAGGACTCTTCTCGAACTCAGGAATCCTGGAATGCCTTCCTGACCAAACTCAG GACTCTGCTGCTCATGTCTTTTACCAAAAACCTTGGCAAGTTTGAGGATGACATGCGGACTTTGAGGGAA AAGAGGACAGAGCCAGGCTGGAGCTTCTGTGAATACTTCATGGTCCAGGAGGAGCTTGCCTTTGTGTTTG AGATGCTGCAGCAGTTTGAAGACGCCCTGGTGCAGTACGACGAGCTGGACGCCCTGTTCTCACAGTATGT GGTCAACTTCGGAGCTGGGGATGGTGCCAACTGGCTGACTTTTTTCTGCCAGCCGGTGAAGAGCTGGAAT GGACTGGTACTCCGCAAGCCCATAGACATGGAGAAGCGCGAGTTGATTCAGAAGCAAGAGGCCACTCTGC TGGACCTGCGAAGCTACCTGTTCTCCCGCCAGTGCACCTTGCTTCTCTTCCTGCAGCGGCCATGGGAGGT GGCTCAGCGCGCCCTGGAGCTGCTGCACAGCTGTGTGCAGGAGCTGAAGCTCTTAGAAGTCTCTGTCCCT CCTGGAGCTCTGGATTGCTGGGTCTTTCTGAGCTGTCTGGAGGTCTTGCAGAGGATAGAAGGTTGCTGTG ACCGGGCACAGATAGACTCCAACATTGCCCACATGGTGGGACTGTGGAGCTATGCCATGGAAAAGCTAAA GTCCTTAGGGTACCTCTGTGGACTTGTGTCAGAAAAAGGGCCCAACTCGGAAGATCTCAACAGGACGGTT GATCTTTTGGCGGGACTAGGAGCGGAGAGACCTGAGACAGCCAACACAGCTCAGAGTCCTTATAAGAAGC TCCAGGAAGCGCTGTCATCAGTAGAGGCCTTTGAGAAGCACTACCTGGACTTGTCCCATGCCACCATTGA GATGTATACAAGTATCGGGCGGATTCGGTCTGCCAAGCTTGTTGGAAAAGATCTGGCAGAATTTTACATG AGGAAAAGGTCGCCACAGAAGGCGGAAATGTATCTTCAGGGAGCTTTGAAAAATTACATGGCTGAGGGCT GGGCCCTGCCTGTCACACACACAAGGAAGCAGCTAGCCGAGTGCCAAAAACACCTGGGGCAAATGGAAAA CTACTTGCAGACCAGCAGCCTCCTGGCCAGCGACCATCACCTGACGGAGGAGGAGCGGAAGCACTTCTGC CAGGAGATCCTCAGCTTTGCCAGTCAGCAGGAGGACAAGGCAGGTCACAAGGTGGTACTGCCCATGCACT CGTTTGCACGGCTAAAGGATCTCCATTTTGACCCTCCCAATGCTGTGGTCCACGCGGGTGGCGTCCTCAC TGTGGAGATAACGGTTTGCAGCCAGATGCCTGTCCCTGTCCATGTGGACCAGATTGCTGTGAACGTCCAC TTCAGCATTGAAAAAAACAACTACCGGAAGACAGCTGAGTGGCTGACCAAGCACAAGACTTCCAATGGAG TCATCACCTTCCCAGCCGAGGCCTCCCTGTTCCCTGCACCTCAGAACAGCCTGCCGGCCCTGGAGCTGTC AGAGATGTTGGAGAGAAGCCCCTCTGATAACTCCCTGAACACCACAGGCATCATCTGTAGGAACGTCCAC ATGCTCCTAAGGAGGCAGGAGAGTGGCTCTTCTCTAGAGCCGCCCTCGGGGCTGGCCCTGGAGGACGGGG CCCACGTGCTGAGGTGCACCAGTGTGACTCTGCAGCCAGGCGCCAACAAGATAGTCTTCAAGACCCAGGC CAAAGAACCTGGAACATATACGCTGCGACAGCTTCGGGCCTCGGTGGGCCCTGTGTGGTTTGTCCTTGCA CACATCCACCCCATTGTACAGTATGATGTGTACTCTCAGGAGCCTCAGCTGCATGTGGAACCGCTGGCTG ATAGCCTTTTGGCCGGCATTCCTCAGAAAGTCAAGTTTACTGTCACTACCGGCCATTACACAGTAAAAGA CGGAGACAGCCTCCAGCTCAGCAATGTGGAGGCCATGCTCATCTTGTGTCAGGCGGAGAACAGGGCCGTG GTCTACTCTAACACAAGAGAAGAGTGCTCCTCTGCCTTGCTCCGGATCCAGTCCTCTGACAAGGTGACCA GCATCGGTCTCCCCACTGCGCCTGCGTACCACGTGATCGAGTTTGAGCTGGAGGTTCTGTCTTTACCTTC AGCCCCAGCAGCTGTCGAGGATTCCAGTATTCTGGGGACACCAGAGCTCCACAGGAAGCAGAAAGATTCT CAGAGAGCTGACCATTGCATGGTCACCACAGATCACAAGGTGTCCATCGACTGCCCGTGGTCTATCTACT CCACTGTCATCGCACTGACATTCAGTGTGCCCTTCAGGACCGCGCACTCTCTGCTGTCCGCTGGAACCCG GAAATATGTGCAAGTCTGTGTTCAGAACCTATCGGAACTGGACTTTGAGCTGTCAGATAGTAATCTGGAA GACAAAGGCCATGCTACTGACCTGAGGCTGGTGCCCCTGAACACACAGTCCCAGCAGCTTATCCACAGCA AGCAGTCCGTGTTCTTTGTCTGGGAGCTGACGTGGACACAGGAGCCACCCCCTCCTCTGCACTGCCGCTT CTCAGTGGGGTTCTCCCCAGCTTCTGCAGAGCGGCTGACTGTCTCCCTGAAGCCCTACACCTACGAGTTC CAAGTGGAGAGCTTTTTTACGCTGTACAGTGTGAGAGCTGAGATCCTTCCTGCCTCAGGAGCCGAGTTCT GTAAGACAGGCTCGCTCTGCTCCTTGGAGGTGTGCATCACACGGCTCGCCGACCTCCTGGATGTGGACAA GGACGAAGCACTGGTGGAGTCTGAGGACTATTTTTCCACAAAGCTTATGTACGAAGTTGTGGACAACAGC AGCAACTGGGCTGTCTGTGGGAAGAGCTGTGGTGTCATCGCCATGCCTTTGGCTGCCCAGGCTACTCACA GAGTCCACATGGAAGTGATGCCTCTGTTTGCTGGTTACCTCCCCTTGCCTGATGTCCGACTGTTCAAGTA CCTTCCCCACCACTCTGCTCATGCCTCTCAACTGGACGCTGACAGCTGGATAGAAAATGACAGCCTGTCA GTGGACAAGCACCTAGACGACCAGCTGGACTGCAGCAGCTTGCGGAGCAGAGGCAGTGCACACTCCACTT CCAGCGGCGAGCACAAAGGCTTGCCGATGCCCCGGCTCCAGGCACTGCCCGCTGGCCAGGTCTTCAACTC CAGCACTGGCATGCAGATCCTGGTCATTCCCAGTCAAGACGACCACGTCCTGGAAGTCAGTGTCACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001173528
Insert Size	3780 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001173528.1, NP_001166999.1
RefSeq Size	6610 bp
RefSeq ORF	3780 bp
Locus ID	309678

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