Phldb1 (NM_001191578) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ll5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217608 representing NM_001191578 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACTCTTAATAGGAACCAGCTAGGCCCTGGATGCAAGACCCAGGCTGTGGTGCAGAAAGGGCCGT TGGATCTGATTGAGACGGGTCAAGGACTGAAGGTGCAGACAGACAAACCTCATCTTGTGAGCCTGGGCAG CGGCAGGCTCAGCACAGCAATCACTCTCCTGCCGCTGGAGGAAGGGAGGACAGTGATTGGTTCAGCAGCC AGGGACATCTCACTGCAAGGTCCAGGCCTTGCTCCAGAACACTGCTACATCGAGAATCTGAGGGGCACTC TCACCCTGTACCCTTGTGGCAATGCCTGCACTATTGATGGGCTCCCTGTTCGGCAGCCCACCCGGCTCAC TCAGGGCTGCATGTTGTGTCTGGGTCAGTCCACCTTCCTGCGCTTTAACCACCCAGCCGAAGCCAAGTGG ATGAAGAGTATGATTCCAGCAGGGGGCCGGGCCCCAGGGTCCACGTACAGTCCTGGCTCTGCTGAATCAG AGAGCTTGGTCAATGGGAACCACACAGCACAGCCCGCAACCCGGGCACCTCCAGCCTGTGCCAGCCACAG TTCCCTGGTGAGCTCCATTGAAAAGGACCTGCAGGAAATCATGGACTCGTTGGTGCTAGAGGAGCCCGGA GCTGCAGGCAAGAAGCCTGCAGCCACATCCCCGTTGTCGCCAGTGGCTAATGGTGGACGCTACCTGTTGT CCCCTCCAACCAGCCCAGGAGCCATGTCTGTGGGCTCCAGCTATGAGAACACCTCTCCGGCCTTCTCTCC ACTGTCCTCACCAGCCAGCAGTGGAAGCTGTGCTAGCCACTCTCCCAGTGGGCAGGAGCCAGGACCTTCT GTGCCCCCGCTCGTGCCTGCCCGTTCCTCCAGCTACCATTTGGCTCTGCAACCCCCACAGTCCCGCCCAA GTGGTTCCCGCTCCTCTGAGAGTCCCCGGCTGGGCAGGAAAGGGGGTCATGAGAGACCTCCCAGCCCTGG TCTCCGAGGTCTTCTGACTGACAGTCCTGCAGCTACAGTCTTGGCTGAGGCCCGCAGAACCACTGAGAGT CCCAGGCTAGGTGGGCAGCTGCCTGTGGTGGCTATCAGCCTGAGTGAATATCCATCTTCCGGTGCCCGCA GCCAACCCACCAGTATTCCTGGCAGCCCTAAGTTCCAGTCTCCAGTGCCTGCTCCCCGAAACAAGATCGG CACACTCCAGGACCGCCCTCCCAGTCCTTTCCGTGAGCCCCCAGGCACAGAGCGGGTACTAACAACCAGC CCCTCTCGACAGCTTGTGGGCAGAACATTTTCAGATGGGTCAGCAACTCGTACCCTCCAGCCACCGGAGA GTCCCCGCTTGGGCCGGCGGGGCCTGGACAGTATGCGGGAACTGCCCCCGTTGAGTCCATCTCTGTCTCG AAGGGCTCTGTCCCCACTGCCAGCCCGGACTGCCCCAGATCCCAAACTAAGCAGGGAAGTGGCAGAGAGC CCCCGGCCCCGGCGCTGGGCAGCCCATGGGACTTCACCTGAGGACTTCTCCCTGACACTAGGAGCACGGG GCCGCAGGACTCGGAGTCCTTCGCCCACACTCGGGGAGTCCCTGGCACCTCGCAAGGGCAGCTTCAGTGG CAGGCTAAGCCCAGCCTACAGTCTGGGCTCTCTTACTGGGGCTTCACCTCGCCAGAGCCCCCATGCTCAA AGGAAGCTTTCCAGTGGGGACTTGCGGGTGCCCATCCCAAGGGAGAGGAAAAATAGCATCACGGAGATCA GCGACAATGAGGACGATCTCCTGGAGTACCACAGGCGGCAGCGCCAAGAGCGGCTCCGGGAGCAGGAGAT GGAGAGGCTGGAACGCCAGCGCCTGGAGACCATCCTGAACCTGTGTGCTGAGTACAGCCGAGCTGACGGG GGACCTGAGACTGGGGAACTGCCCAGTATAGGAGAGGCCACGGCAGCACTGGCTTTGGCAGGCAGGAGGC CCTCAAGAGGCTTGGCAGGAGCCATAGTAGTGTCTGGAAGGAGTGGTGAGGAGTCTGGAGGTGCCTCGCA GCGCCTGTGGGAGAGCATGGAGCGCTCTGATGAAGAGAATCTCAAAGAGGAGTGCAGCAGCACAGAGAGC ACCCAGCAGGAGCACGAAGATGCTCCTAGCACGAAGCTCCAAGGAGAGGTGCTGGCTGTGGAAGAGGAGC GGGCTCAGGTTCTGGGGCGCGTAGAGCAGCTGAAGGTCCGAGTGAAGGAGCTGGAACAGCAGCTGCAGGA GGCAGCCCGAGAGGCTGAAATGGAGAGAGCACTGCTGCAGGGGGAGAGGGAGGCGGAGCGAGCATTGCTG CAGAAGGAACAAAGAGCTATGGACCAGCTGCAGGAGAAGTTGGTGGCCTTGGAGACAGGCATCCAGAAGG AGAGGGACAAGGAGGCAGACGCTCTAGAGACTGAGACAAAGCTCTTTGAGGACCTGGAGTTCCAGCAGCT GGAGCGGGAGAGTCGCGTGGAGGAGGAGCGGGAGTTGGCCGGCCAAGGGCTGCTTCGCAGCAAGGCTGAG TTGCTGCGCAGCGTGAGCAAGAGAAAGGAGCGCCTAGCTGTACTGGACAGTCAGGCTGGGCAGATCCGGG CCCAAGCAGTACAGGAGTCAGAGCGTCTGGCCCGGGAAAAGAATGCTGTCCTGCAGCTACTGCAAAAGGA GAAGGAGAAGCTGACTGTGCTTGAGAGGAGATATCATTCGCTCACGGGTGGCAGGTCTTTTCCGAAGACC ACCTCAACCCTCAAAGAGATGGAGAAACTGCTGCTCCCTGCTGTAGACTTAGAGCAGTGGTACCAGGAGT TGATGGCCGGGCTGGGGACTGGCCTTGCTGCAGCCTCCCCTCGCTCCTCTCCCCCGCCTCTGCCTGCCAA AGCTTCTCGTCAGCTGCAGGTTTACCGCTCCAAGATGAATGGTGACATGGCCAGCCCCCTGCCCAGGACC CGTAGTGGCCCTCTCCCCTCCTCCTCAGGCTCCTCATCCTCTTCTTCACAGCTCAGCGTGGCTACCCTGG GCCGTAGTCCTTCCCCAAAGAGTGCCCTGCTCGCCCAGAATGGCACCAGTAGTCTCCCTCGAAACCTGGC AGCCACGCTGCAGGACATTGAGACCAAGCGCCAGCTGGCCCTACAGCAAAAGGGGCACCAAGTGATCGAG GAGCAACGGCGACGGCTGGCTGAGCTGAAGCAGAAAGCAGCGGCAGAGGCTCAGTGCCAGTGGGATGCCT TGCATGGTGCAGCCGCCTTTCCAGCAGGCCCGTCTGGCTTCCCCACACTCATGCACCATTCTATCCTGCA CCACCTGCCAGCAGGCAGGGAACGTGGAGAGGAGGGCGAGCACGCTTACGACACCCTGAGCCTGGAGAGT TCGGACAGCATGGAGACCAGCATCTCTACGGGGGGCAACTCCGCCTGTTCCCCTGACAACATGTCCAGCG CTAGTGGTCTGGACATGGGCAAGATAGAGGAAATGGAGAAGATGCTAAAGGAAGCTCACGCGGAGAAGAG CCGGCTCATGGAGTCCAGGGAGCGGGAGATGGAGCTACGGAGGCAGGCCCTGGAGGAGGAACGTCGGAGG CGGGAGCAAGTGGAGCGGAGGCTGCAGAGTGAGAGCGCCCGCAGGCAGCAGCTGGTGGAGAAAGAAGTCA AGCTGAGGGAGAAGCAGTTTTCCCAGGCTCGACCTCTGACACGTTATCTTCCCAACCGGAAGGAGGACTT TGATTTGAAGACCCACATAGAGTCGTCAGGCCATGGGGTGGACACCTGCCTACATGTGGTGCTTAGCAGC AAGGTCTGCCGTGGCTACTTAATCAAGATGGGTGGCAAGATTAAATCGTGGAAGAAGCGCTGGTTTGTCT TTGACCGGCTGAAGCGCACCCTTTCCTACTATGTGGACAAACACGAGACGAAGCTGAAGGGGGTCATCTA CTTCCAGGCCATCGAGGAAGTGTACTATGACCACCTGCGCAGCGCAGCCAAGAAAAGGTTTTTCCACTTC ACCATGGTGACTGAGAGTCCGAATCCAGCCCTCACCTTCTGTGTGAAGACCCATGACCGGCTGTACTACA TGGTGGCCCCATCTGCAGAGGCCATGCGCATCTGGATGGATGTCATCGTCACGGGAGCTGAGGGCTACAC TCAATTCATGAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191578
Insert Size	4146 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191578.1, NP_001178507.1
RefSeq Size	5398 bp
RefSeq ORF	4146 bp
Locus ID	171434
Gene Summary	expressed in pituitary [RGD, Feb 2006]

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