Dip2a (NM_001191564) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217657 representing NM_001191564 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACCGCGGATGCCCTCTGGAGGCGGCGCCGCTGCCCGCCGAGGTGCTCGAGAGCCTGGCGGAGC TGGAGCTGGAGCTGTCCGAAGGTGACATCACTCAGAAAGGATATGAGAAGAAGAGGGCGAAGCTGCTGGC TCGTTATATCCCCCTCATTCAAGGAGTGGACCCCTGTCTGCAAACAGAGAGTAGAGTCCCTGGGCCCCTG CTGACTGCACCTACAGCTAAACCACAGAAGCCTCGGGCCAACTCGAGGGATGAGCGGTTCCGGTCAGATG TTCACACGGAAGCCGTGCAGGCAGCTCTGGCCAAGTACAAGGAGAGGAAGATGCCCATGCCTTCAAAGAG ACGGTCTGCCCTTGTGCACTCATCCGTGGAAACCTATACACCTCCAGACACGTCATCTGCCTCAGAAGAT GAGGGCTCTTTACGGCGACCTGGGCGACTCACCTCCACTCTGCTCCAGAGCCATTCCGGCATCGAGCCCT GGCTCGACAGGGTCATTCAGGGCTCGTCCACTTCATCCTCTGCATCCTCCACCTCATCCCACCCGGGAGG GAGACCTGCTGCTGCTCCCAGTGCCTCCACTGCGCTTGCAGGCCTCACGGCCCACGCCCACATAGATCCG CACTCCGCCCCACCCGATGTTACCACTGGGCTTGTGGAACACTCACACTTCGAGCGTCCACAGATGGCCT CTGTACGAGGTGTTCCTCGAGGGCACGGCAGGAACATGCTGGAGACCGCAGATGGTGTGCCTGTGAACAG CAGAGTGTCCTCCAAAATCCAGCAGCTATTGAATACCCTGAAGAGGCCAAAACGCCCTCCTCTGAAGGAG TTTTTTGTGGATGATTTTGAGGAGTTACTGGAAGTTCAGCAACCGGATCCCAATCAGCCCAAGCCGGAGG GGGAGCAGATGGCTGTGCTGAAGGGAGAACCTCTGTCAGTGGGGACTTCGGGGCCACCAACTCTGTTGGC TGCACTGCAGCTGTGGGGCACAACACAGCCTAAGGCCCCCTGCCTGACTGCCTTGGATACATCGGGGAAA GCCATCTGCACCCTCACCTATGGCAAACTCTGGAGTCGGAGCTTAAAATTAGCTTATACTCTGCTCAGTA AGCTGACTAGTAAGAATGAGCCACTGCTTAACCCTGGAGACAGGGTGGCACTCGTGTTTCCAAATAGTGA CCCTGTAATGTTTATGGTTGCGTTTTACGGGTGTCTCCTGGCGGAGCTGGTCCCTGTCCCTATAGAAGTA CCATTAACGAGAAAGGATGCAGGCAGCCAGCATGTTGGCTTTCTCCTGGGCAGCTGCGGGGTGACCCTGG CCCTGACCACAGATGCTTGTCAGAAGGGCCTGCCCAAGGCGCCGACAGGAGAGGTGGCAACTTTCAAAGG TTGGCCCCCCCTGGCCTGGCTGGTGATTGATGGGAAGCATCTGACCAAGCCTCCGAAGGACTGGTACCCG CTGGCCCAGGACACAGGGTCCAGGACCGCCTACATTGAGTATAAAACCAGCAAGGACGGCAGCACAGTAG GGGTCACCGTACCCCACTCATCCCTGCTGGCGCAGTGCCAGGCCCTGACCCAGGTGTGTGGGTACACGGA AGCCGAAACATTAACGAATGTGCTGGACTTCAAAAGGGATGCTGGTCTGTGGCATGGAGTCTTAACAAGT GTCATGAACAGGATGCACGTTATCAGCATCCCCTACGCCCTAATGAAGGTCAACCCCCTCTCCTGGATCC AGAAAGTGTGTTCCTATAAAGCCCGGGCTGCCCTGGTGAAGTCCCGAGATATGCACTGGTCACTCTTGGC ACAGCGAGGTCAGAGGGACGTCTGTCTCAGCTCTCTGCGTATGTTGATTGTGGCGGATGGCGCAAACCCA TGGTCAATATCTTCCTGCGATGCCTTCCTCAACGTCTTCCAGTCTAGAGGTCTGAGGCCAGAGGTCATCT GTCCTTGTGCCAGTTCTCCTGAGGCGCTAACAGTAGCCATCCGCAGGCCCCCAGACCTGGGAGGACCTCC ACCAAGGAAAGCTGTCCTGTCCATGAACGGCCTGAGCTACGGTGTGATCAGAGTTGACACCGAGGAGAAG CTGTCGGTCCTCACTGTTCAGGATGTCGGCCAGGTGATGCCTGGAGCTAGTGTCTGTGTCGTGAAGGTAG ACGGTGTCCCTTACCTCTGTAAAACTGATGAAATCGGAGAGATCTGTGTCAGTTCTGTGGCCACTGGGAC AGCATATTACGGGCTGCTTGGCATCACAAAGAATGTCTTTGAGACCGTCCCGGTCACTGCGGATGGAGTC CCTGTCTCTGACCGGCCTTTCACCCGGACAGGTCTTCTGGGGTTTATTGGGCCTGAGAACCTGGTCTTCG TCGTGGGCAAGCTGGACGGGCTGACGGTCGTAGGAGCACGCAGACATAATGCAGATGACATTGTGGCTAC TGCCCTGGCTGTGGAACCCATGAAGTTTGTCTACAGAGGCAGGATCGCCGTGTTCTCTGTGACTGTTCTG CACGATGACCGGATTGTCTTGGTGGCCGAACAGCGGCCCGACTCTTCGGAGGAAGACAGCTTCCAGTGGA TGAGCCGTGTGCTACAGGCCATTGACAGCATCCACCAGGTGGGCGTGTACTGTTTGGCCCTGGTTCCCGC CAACACCTTGCCCAAGGCTCCTCTTGGAGGAATTCACATTTCTGAAACGAAGCAACGCTTCCTGGAAGGG ACGCTACACCCATGTAACGTGTTGATGTGCCCTCACACATGCGTTACCAACCTTCCCAAGCCTCGCCAGA AACAGCCAGAAGTCGGACCAGCCTCCATGATTGTTGGGAACCTGGTTGCCGGAAAGAGGATCGCACAGGC CTCTGGGAGAGAGCTGGCCCACCTGGAGGACAGTGACCAGGCTCGCAAGGTACGTGCGAGGCTTCCTCTC TCTCTGCAGTTCCTGTTCCTGGCGGACGTGCTGCAGTGGAGGGCTCACACCACTCCCGACCACCCGCTGT TCTTGCTCCTGAATGCTAAGGGCACAGTCACCAGCACGGCAACCTGCATCCAGCTGCACAAAAGGGCTGA GAGAGTGGCTGCCGCGCTGATGGAGAAGGGCCGACTAGATGCTGGGGACCATGTGGCTTTGGTGTATCCT CCAGGGGTGGATCTTATTGCTGCGTTCTATGGCTGCCTGTACTGTGGCTGTGTGCCCGTCACTGTGCGAC CCCCACACCCCCAGAACCTAGGCACCACACTGCCCACTGTGAAGATGATTGTCGAGGTCAGCAAATCTGC ATGTGTCCTTAGCACACAGGCCATTACACGGCTGCTCAAGTCCAAAGAAGCAGCTGCGGCTGTAGATGTC AGGACCTGGCCAACCATCCTAGATACAGATGACATTCCAAAAAAGAAGGTAGCTAGCATTTTCAGACCAC CCTCCCCAGATGTGCTTGCATACTTGGACTTCAGTGTGTCGACGACAGGGATCTTAGCGGGAGTTAAGAT GTCACACGCAGCCACAAGTGCCTTGTGCCGCTCTATAAAGCTTCAGTGTGAGCTGTACCCCTCACGGCAG ATTGCCATCTGCCTGGACCCCTACTGTGGCCTTGGCTTCGCCCTGTGGTGTCTGTGCAGCGTGTACTCTG GACACCAGTCGGTACTCGTCCCCCCACTGGAGCTGGAGAGCAATGTGTCTCTCTGGCTGTCCGCCGTCAG CCAGTACAAGGCCCGAGTCACCTTCTGTTCCTACTCAGTGATGGAAATGTGCACCAAGGGCCTTGGTGCT CAGACAGGCGCCCTCAGGATGAAGGGGGTGAACCTGTCGTGTGTGCGCACGTGCATGGTGGTGGCTGAGG AGCGGCCCCGGATCTCGTTGACACAGTCCTTCTCCAAGCTATTCAAAGACCTGGGCCTCCCTGCTCGCGC TGTGAGCACCACCTTCGGGTGCAGGGTCAACGTGGCCATCTGCCTCCAGGGCACAACAGGCCCAGATCCC ACGACGGTCTATGTGGACATGCGGGCACTGCGCCATGACAGAGTTCGTCTGGTGGAACGGGGTTCTCCGC ACAGTCTGCCGCTGATGGAGTCTGGGAAGATTCTTCCAGGAGTGAAGGTCATCATCGCACACACTGAGAC CAAAGGGCCCCTCGGAGACTCACACCTGGGTGAGATCTGGGTGAGCAGCCCCCACAATGCCACTGGGTAC TACACGGTCTATGGGGAGGAGGCACTTCATGCTGACCACTTCAGTGCCCGGCTGAGCTTTGGAGACACCC AGACCATTTGGGCAAGGACTGGCTACCTTGGCTTCCTTCGCAGGACAGAGCTGACTGATGCCAGTGGAGA GCGACACGATGCGCTGTATGTTGTCGGCTCTCTGGACGAGACGCTGGAGCTGAGGGGCATGCGCTATCAC CCCATCGACATCGAGACCTCTGTGATCCGGGCACACAGGAGCATCGCGGAGTGTGCCGTGTTCACCTGGA CCAACCTGCTGGTGGTGGTGGTGGAGCTCGATGGTCTGGAGCAGGATGCCCTGGACCTGGTGGCCCTGGT GACCAATGTCGTGCTGGAGGAGCACTACCTTGTGGTGGGCGTGGTGGTCATTGTGGACCCTGGAGTGATC CCCATCAACTCCCGGGGCGAGAAGCAGCGCATGCACCTGCGCGACGGCTTCCTGGCCGACCAGCTGGACC CCATCTATGTGGCCTACAATATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191564
Insert Size	4716 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191564.1, NP_001178493.1
RefSeq Size	5445 bp
RefSeq ORF	4716 bp
Locus ID	690211

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