Tiam1 (NM_001100558) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217661 representing NM_001100558 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAACGCAGAAAGCCAAAATGTAGACCACGAGTTTTATGGAGAAAAGCATGCCAGCCTGGGGCGGA AGCACACATCACGCTCCCTGCGACTGTCACACAAGACCCGGAGGACGCGGCATGCTTCCTCTGGGAAGGC AATCCACAGGAACTCTGAAGTGAGCACGCGGTCCAGCAGCACCCCTAGCATCCCCCAGTCCCTGGCCGAA AATGGCTTGGAGCCCTTTTCCCAAGAAGGCGCCCTAGACGACTTCGGGGACCCCATCTGGGTGGACCGAG TGGATATGGGCTTGAGACCCGTGTCTTACACGGATTCTTCTGTCACTCCCAGCGTAGACGGCAGCATCGT CCTCACGGCAGCCTCTGTGCAGAGCATGCCAGACTCGGAGGAGAGCCGGCTTTACGGGGATGACGCTGCG TACTTGGCTGAGGGAGGCAGGAGGCAGCGTCCCTATACATCCAACGGGCCCACGTTCATGGAGACAGCGA GCTTTAAGAAGAAACGCTCTAAATCTGCAGACATCTGGCGGGAGGACAGCCTGGAGTTCTCACTCTCAGA TCTGAGCCAAGAACATTTAACAAGCAACGAAGAAATCTTGGGCTCCGCGGAAGAGAAGGATTGCGAGGAG GCTCGGGGGATGGAAACAGGGGCGAGCCCCCGGCAGCTCAGCACCTGTCAGCGAGCCAACTCCCTGGGTG ACTTGTATGCTCAGAAGAACTCCGGGGTGAAGGCTAACGGAGGACCGAGAAACAGATTTTCGAGCTACTG CCGGAATTTGGTGTCGGATATTCCCGATCTTGCAAAGCATAAGATGCCACCGGCTGCTGTAGAGACTCCG ACGTACAGTAATTATAACACGCTTCCCTGTAGGAAGTCGCACTGTCTTTCCGAGGGTGCCACCAACCCAC AAATTAGCCTTAGCAAGAGCATGCAAGGCAGAAGAGCGAAAACCACCCAGGATGTTAACACAGGCGAGGG CAGTGAGTTTGCAGACAGTGGGATCGAAGGGGCCACCACGGACACGGACCTCCTGTCCAGGAGATCCAAT GCCACCAACTCCAGCTACTCGCCTCCCACAGGCCGAGCCTTTGTGGGCAGCGACAGCGGCAGCAGTTCGA CTGGGGATGCTGCACGCCAGGGGGTGTACGAGAACTTCAGGCGGGAACTGGAGATGAGCACCACCAACAG CGAGAGCCTGGAGGAGGCTGGCTCTGCGCACAGCGACGAGCAGAGCAGCGGAACCCTCAGCTCCCCGGGC CAGTCGGACATCCTGCTGACGGCTGCGCAGGGCACTGTGCGGAAGGCTGGCGCGCTGGCAGTCAAGAACT TCCTGGTACATAAGAAGAATAAGAAGGTGGAGTCGGCTACCCGGAGGAAGTGGAAGCACTACTGGGTGTC CCTGAAAGGTTGCACGCTCTTTTTCTACGAGAGTGATGGCAGGTCTGGGATAGACCACAACAGTGTCCCC AAGCATGCTGTCTGGGTGGAGAACAGCATCGTGCAGGCCGTACCTGAGCACCCCAAGAAAGATTTTGTCT TCTGCCTCAGCAACTCCCTGGGCGATGCCTTCCTCTTCCAGACCACCAGCCAGACAGAGCTGGAGAACTG GATCACGGCCATCCACTCTGCCTGCGCAGCTGCGGTCGCAAGGCACCACCACAAGGAAGACACGCTCCGC CTCCTCAAGTCGGAAATCAAGAAGCTGGAGCAAAAGATTGACATGGACGAGAAAATGAAGAAGATGGGCG AGATGCAGCTGTCCTCCGTCACGGACGCGAAGAAGAAGAAGACCATACTTGACCAGATCTTCGTTTGGGA GCAGAACCTCGAACAGTTCCAGATGGACCTTTTCCGGTTCCGCTGTTACCTTGCCAGCCTCCAGGGCGGG GAGCTGCCAAACCCCAAACGACTCCTAGGTTTTGCCAGCCGGCCCACCAAGGTGGCCATGGGCCGCCTTG GCATCTTTTCGGTATCATCTTTTCACGCCCTGGTGGCGGCCCGCACTGGCGAGATCGGGGTGAGAAGGCG TACTCAGGCTATGTCCAGGTCTGCGAGCAAGCGGAGGAGCAGGTTTTCTTCTCTCTGGGGCCTAGACACT ACCTCCAAAAAGAAGCAGGGGCGCCCAACCATCAACCAGGTGTTTGGAGAGGGGACCGATGCTGTAAAGA GATCTTTAGAGGGAATATTTGATGACTCTGTTCCAGACGGCAAGAGGGAGAAGGAAGTGGTCTTACCCAG TGTCCACCAGCACAATCCTGACTGTGACATTTGGGTCCATGAATATTTCACTCCATCCTGGTTCTGTCTA CCCAATAATCAGCCAGCCTTGACGGTTGTCCGGCCGGGGGACACTGCGAGGGACACCTTGGAGCTCATTT GCAAGGCGCACCAACTGGATCATTCTGCCCATTACCTGCGCCTGAAATTTCTGATGGAGAACAAGATGCA GTTCTATGTCCCGCAACCCGAGGAGGACATTTACGAGCTGCTTTACAAAGAAATCGAAATCTGTCCGAAA GTCACCCAGAACATCCACATTGAGAAGTCCGACGCGGCCGCCGATAATTACGGGTTTTTGCTTTCTTCTG TGGACGAAGATGGCGTTCGAAGGCTCTACGTGAACAGCGTCAAGGAAACCGGGCTAGCTTCCAAGAAAGG CCTGAAGGCGGGGGACGAGATTCTTCAGATCAATAACCGTGCCGCTGGTACCCTGAACTCATCTATGCTC AAAGATTTCCTCTCACAGCCCTCTCTGGGCCTCCTGGTGAGGACCTACCCTGAGCCAGAGGGAGGAGTGG AGCTGCTAGAGAACCCACCCCACCGAGTGGACGGCCCTGTGGACCTTGGCGAGAGCCCCCTCACCTTCCT CACCAGCAACCCAGGGCACAGCCTCTCCAGCGAGCAGGGCAGCAGTGCTGAGACAGCTCCGGAAGAGGGC GAAGGACCGGACTTGGAGTCTTCCGATGAGACAGATCACAGCAGCAAGAGCACAGAACAGGTCACAGCGT TTTGCCGGAGTCTGCACGAGATGAGCCCCTCTGACTCGAGCCCGTCCCCTCAGGATGCCACGAGCCCTCA GCTGGCAACCACACGACAGCTATCGGACGCAGATAAACTGCGCAAGGTGATCTGTGAGCTCCTAGAGACC GAACGCACCTATGTGAAGGACTTAAACTGCCTCATGGAGAGATACCTGAAGCCCCTTCAGAAGGAGACCT TTCTCACCCAGGATGAGCTCGATGTACTCTTTGGAAATTTAACCGAAATGGTGGAGTTTCAAGTTGAGTT CCTTAAAACTCTAGAAGATGGAGTTAGACTGGTCCCTGACTTGGAAAAGCTTGAGAAGGTTGACCAGTTT AAGAAAGTGCTGTTCTCTCTGGGGGGATCCTTCCTGTACTACGCGGACCGCTTCAAGCTCTACAGTGCCT TCTGTGCGAGCCATACGAAAGTCCCCAAGGTCCTGGTGAAAGCCAAGACGGACACAGCCTTCAAGGCATT CCTAGATGCCCAGAACCCGAGGCAGCAGCACTCGTCCACACTAGAGTCTTACCTCATCAAGCCCATCCAG AGGGTCCTCAAGTACCCGCTTCTGCTCAGGGAGCTGTTTGCGCTGACCGACGCGGAGAGTGAGGAGCATT ACCACCTGGATGTGGCCATCAAGACTATGAACAAGGTTGCCAGTCACATCAATGAGATGCAGAAGATCCA CGAAGAATTTGGTGCTGTGTTCGACCAGCTGATTGCTGAGCAGACAGGAGAGAAGAAAGAGGTCGCGGAT CTGAGCATGGGTGACCTGCTGTTGCACACCAGTGTCATCTGGCTGAACCCACCCGCCTCGCTGGGAAAGT GGAAAAAGGAGCCGGAACTGGCAGCCTTCGTCTTCAAAACTGCCGTGGTCCTTGTGTATAAAGATGGTTC CAAGCAGAAGAAGAAGCTCGTTGGCTCTCACAGGCTGTCAATCTACGAGGAGTGGGACCCTTTCCGGTTT CGCCACATGATCCCTACCGAAGCTTTGCAAGTCCGAGCTCTGCCCAGTGCAGATGCAGAGGCAAATGCCG TGTGTGAAATTGTCCACGTGAAATCAGAGTCAGAGGGGAGGCCGGAGCGGGTTTTCCACCTCTGCTGCAG CTCCCCGGAGAGCCGAAAGGATTTTCTGAAGTCTGTACACTCCATCCTGCGAGATAAGCACAGAAGGCAG CTCCTCAAAACGGAAAGTCTTCCCTCAGCCCAGCAGTATGTCCCTTTTGGAGGCAAGAGATTGTGTGCGC TTAAAGGCGCCAGGCCAGCCATGAGCAGGGCAGTGTCTGCCCCAAGCAAGTCTCTTGGGAGGAGGAGGCG GCGACTGGCCCGAAACAGGTTTACCATTGATTCAGACGCCATCTCAGCCAGCAGCCCGGAGAAAGAGCCC CAGCAGCCCCCCGGTGGCGGGGACACTGACCGATGGGTAGAGGAACAGTTTGATCTTGCTCAGTATGAGG AGCAGGATGACATCAAGGAGACAGACATCCTCAGTGACGATGATGAATTCTGTGAGTCTGCGAAGGGCGC CTCAGTGGACATAGACCTGCAGGAGCAGCTTCAGGCTGCCTCTATCAGTCAGCGCGCCCGAGGCCGGAAA ACCCTTGATAGCCACGCCTCCCGCATGACACAGCTCAAGAAGCAAGCGGCCCTTTCGGGCATCAACGGAG GCCTGGAGAGTGCGAGCGAGGAGGTCATTTGGGTTAGGCGCGAAGACTTTGCCCCCTCCAGGAAACTGAA CACAGAGATATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100558
Insert Size	4773 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100558.1, NP_001094028.1
RefSeq Size	7036 bp
RefSeq ORF	4773 bp
Locus ID	304109

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