Rnf17 (NM_001191685) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217675 representing NM_001191685 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAGAGGCTTCGTCGACTGGGTTGGCTTCCTGTCACCTAGCTGGGAGTAAGAGTGGAGCGCCGG GTGCTTCAGGGTGCCAGTGCACTCGGTGTGGAAGGAAGGCGTCCTTTGCCTCTGGTCACCACCACGAGTA TCCATGTGGTCATGCCTTTTGTGAACTGTGCCTGTTAGCACCTCAAGAATACACCACGAGTAAATGCACT GACTGTGAGGTTCGTACAACTGTCACTATGAATCAAGGTCACTACCCAGTAGATGGATTTATTAAAGAAG ATTCTTCTGTGGAAGCATTGCCACCTAAAATGGTAAATAACTGCTCTTCAGGTCTTGAAAAGACAGTGGA CCAGCTTATTATCAATTTAGAACATTCATCCTCCATACACAGGAATGTTTCAAGCCCATCAGCTGTAATG TCGGAGACAGAAGAAATTGATGAAGCACTGAAGATAGCAGGCTGCAATTCTGAACAATTAAGTAATGCTA TAAAAATGCTTGATAGCACACAAGATCAAACAAGACAAGAGACGGGCAGTCTAAGAGAGGTTGTGGAGAA ACAATTCGATACACTTATTGCTTCTCTTGATTCCAGGAAAAAGAGCCTGTGTGAAGAACTTGTAAGACGT ACAGATGATTATTTATCAAAATTAGTAACAGTTAAAAGCTACATTGAAGAGAAGAAAAGTGACTTGGATG CAGCCATGAAGATAGCAAAAGAACTCAAATCAGCTCCTTCTCTGAGGACCTACTGTGACCTGACTCAGAT TATCCGGACCTTGAAGTTAAACTTTGAAAGTGAACTATCACAAGTTAGTTCCATAATTCCAAGGAACCCT CCCAGGTTGAATATAAATTGCAGTGAGGTTATCTGCATGTTCAACAGTATGGGAAAGATTGAATTTGAGG ACTCAACGAAATGTCACCCTCAAGAAAATGAAGTTGGACAGAATGTTCAAAAGAAATTTAATAGTAAAAA GGAACTGTGTTGTGATGTATACTCCTCACTAGAGAAGAAAAGGGTAGACACGGTCGTCCTGGCTGATGAA GCCCCTGTACCTCCTTTGCAAGCAGAGACCCCTGACAGGCATTTGGAAGGGGAAAACAAGCAGCCACAGA AAGAGGTGCTCGTGGTGGCTTCTCCTAAGACTGTTGCTGTACTGCCTCAACTGGGATCCAGCCCTGATGT GATAATTGAAGAAATTATTGAGGAAAACCTAGAATCATGCTTCACAGATGAACCTATAGAGACTCCTAGA TACCCAAAAAAGCCTCCTCAGAAAGAGCTGTCTGCTCCTGTTGCATCAAAAGCAGGTTGTCCAGAGCTAG TTTTTGTAAGTCATGTCACACATCCTTGCCACTTCTATGTGCGAAAATATTCACAAATAAAAGATGCAGC CATGTTGGAGAAGAAGATGAAGCAGGTGTGCAGTAAGAGCTTGCACCTCGATCCCTCCGACACTCTGGAG CTGGGTGCAAGAATATTTGTCAACAGTATTAAGAATAGAATGTGGTGTCGAGGAATTATCACTGAAATAA TTCCAACAAAAACCAAAAATGTTAGAAAACCATGTAGTCCAACCAAATTCTCAGTCTGTGAAATTTCACT AATACAGATATTCATGGTAGATTTTGGAAATTCTGAAGTCCTGATCATCACGGGAGTTGGTGACACACAT GTGGGACCAGAGCATGTTGGTGAGCAGCATATTACACTAAGTGACTTCTGTCTGCTTGTAAGGAAGTCGG AACCATATAGTGAAGAACTGTTGAAAGACATCCCACATTTAGCACACCTGTGCTCCTTGAAAGACATCGT TCCATACAATTCAAGTGAGGGCTGGGAAAAGGAGGCAAAAGTGGAATTTTTGAAAATGGTAAATAAGAAG GCTGTGTTAATGAAAGTTTTTGGAGAAGAAGATGATATCCTTATTGTAGATCTGCAGAAACCGCCAACAA ATAAAATCAGCAGTGATATGCCTGTGTCTCTCAGAGATGCATTGGTTTTTATGGAACTAGCAAGGTTTAG GTCACAATCACTGAGAAGTCACGGTGAAAACAATACAGCTTTATGCTATCGCCCACCCGCTTTGCCTGAA GAGATGACAGAAGTTTCAGTCATGGTTTGCCATATCAACAGCCCTACTGACTTCTATCTTCAGTTGATGG AGAACCTGGACTTTTTATCTCTTTTAAAAACAATTGAGGAATTCTATAAAGGTGAAGATGGAGAAAATCT GGAAATCCTTTGTCCTCTTCAGAATCAAGCCTGTGTAGCTAAATTTGAAGATGGAATTTGGTATCGTGCA AAAGTGATCGGATTGCCTGGACATCGAGAAGTAGAAGTTAAATATGTGGACTTTGGTAATACTGCAAAAA TAACACTTAAAGATATGCGGAAAATAAAGGATGAGTTTTTGGAACCTCCAGAGAAGGCAATTAAATGTAA ACTGGCATTTATTGAACCAAGTAAAAGATCACACTGGTCCAAAAAGGCTAAAGAAAAATTTGAAGAAAAG ACTCAAGATAAATTTGTGACATGTTCTGTTATTAAGATCCTGGAAAACGGTGTGCTCTTGGTTGAGCTTT TCGATTCCCGAGCTCCTGGAACAAGCGCTGTTAGCATTAATGAGCAGCTTGTGAAAGAAGGCCTAGCATC TTATGAAGCAGGATATACCCTGAAAGATAATTCTAAAAAGCATCTTGAAGTATGGGACCCTTCTCCTGAA GAAATCATTTCAAGTGAAATAAACAGCTTAAGTCCTCTCTCTGTTAACTCTCTGGCTAACGAGAGTTTCC AGTCGTTGTACAACAAGGAGCTGCCTGTGAGCATATGTAATGTGCTATCCCCGGAGAAGATTTACGTCCA GTGGTTGTTAACAGAGAACCTACTGACGAGTTTAGAGGAGAAGATGGTAGCTGCTTACGAGCACTCCGAA TGGAAGCCTGTGAAGTGGGAGTGTGATATGCACTGCGCTGTTAAGATCCCGGCTAAAAGTCAGTGGCGAA GAGGCCAGATCATCAGGATGGTTACAGACACACTGGTAGAGGTCTTGCTGTATGATGTCGGTATTGAACT AGTAGTGAATATTCACTGCTTAAGAGAACTTCAAGAAAATCTAAAGACAATGGGAAGATTATCTCTGGAA TGTTCACTTGTTGATATAAGGCCAACTGGGGGAAGTGACAAGTGGACAGCAACAGCTTGTGACTGTCTTT CATTATACCTCACTGGAGCTATAGCAACCATAATCTTACAGGAAAACAGCACAACGTGGCCATTGCCTGT GAAAATTTTCTGCAGAGATGAAAAAGGAGAACGTGTTGATGTTTCTAAATATTTGATTAAAAAAGGTTTG GCTTTGAGAGATAGAAGAGTGAGTAAATCAAGTAACAGCCATTCGCCTGAGAAGTCTCTGGAAATACCCC TGGAGCAAGGAGATTCAGTAGTTACTAAGTGCTTTCAAATTAACTTTGATACTAACAAGAAAATTGCTGA TAAAGTCAATGAACACAAAGTACCCGAATCTAAGGAAAAATATTCAGAAAGCAGAACCAACGGATTCTAT AAACCACCAGCTGTTCCTAACACGAGTTCATTTGAGGCAATAGTGAGCTGCATTGGTGATGATGGAACTA TATTTGTAGTGCCTAAATTATCAGAGTTTGAGCTCATAAAAATGATGGATGAAATTCAAAGTAATTTAAA GTGCCTTGGCCTTTTGGAGCCATATTTCTGGAAAAAGGGAGAACCCTGTGCAGTGAGAGGATCTGATACT TTGTGGTACCGCGGCAAAGTCATGGAGGTTGTGGGAGGCACCATCCGGGTACAGTACTTAGACCACGGGT TCACGGAGAAGATCCCACAGTGCCATCTCTATCCCATTCTGCTGTATCCCGACACACCCCAGTTTTGTAT TCCCTGTCAGCTCTATCAGACTTTACCGGTTGGGAATGTCTGGCAGCCAGATGCCATAGAGCTGCTTCAG GAGCTGCTTTCCAAGAGAGAGGTGGACATTCACATTATGGAATTACCTGACCATCCATGGGGGAAGTTGT CTGTCCATCTCTATTTTGATGGGATGTCACTTTCTTATTTTATGGCCTATCATAAATACTGTATTTTTGA ACATACTGAGGAGATATTTAAAGAAAAACCAAGAGGTCAGAATAAAAAGTACGAAGATGAAAACTGGAAA ATAAGATTTGAGGACTTGCTTTTGCCTGAAATGGAGGCTCCCATTTTACCACCATATCTGTCTTCACTTC TGCCTCCACCAGAGGAGCTTTATGCGGTGCAAGTGAAGCATATCGTCTCACCTGATGAAGTGTATATTTG CCTGGACTCTGTAGAAAGCTGTACTCAGCTTAACAATCATGGTGACACGGATGACAGTGGAGTCAGCTGG GAGCCGGAGTCTGAGAGCCTGGAGGAAGCACTGCAGAGATTGAACAAGAATGTGGAGACCTTTCCTCCCC TCACGGATTTTAGCTCAGAGATGCCTTGCCTTGCAGAATATGCTGATGGTTTATGGTATAGAGCAAAAAT TGTTTCCATTAAGGAATTTAACCCTCTATCTGTCCTGGTATTGTTTGTTGATTATGGGTCCACAGAGAAG TTGACAATAAACAGATTACGTCAGATTCCTATTCAGCTTATGCAGTACCCAGCCCAAGCCATAAAGGTGC TCTTGGCAGGGTTTAAACCTCCCTTGAGTGACTCAGGAAAAACAAGAATACCCTACTGTCCCAGGTGGAG CATGGAGGCGTTGTGGACTATGATAGACTGTCTCCAAGGGAAACAACTTTATGCTTCCTCTGTGGCTCAG GCACCAGAACAGATAGTGACATTATATGAAGATGAACAATACCCAGTTCACATGTCCTTGGTAGAAATGG GGCTTGCAGATAAAGATGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191685
Insert Size	4923 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191685.1, NP_001178614.1
RefSeq Size	5147 bp
RefSeq ORF	4923 bp
Locus ID	305908

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