Sptbn4 (NM_001100845) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Spnb4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217779 representing NM_001100845 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACAAGTACCAGGGGAAGTGGACAACATGGAGGGCCCGGCAGCCTCCAACAACAACAACCCTGCAG CCCGCTGGGAGAGCCCAGATCGGGGCTGGGACCGGGAGCCTCCGGCAGCCGCCAACGCTGCCGCTTCGCT CTTCGAGTGTTCTAGGATCAAGGCCTTAGCAGATGAACGAGAGGCTGTGCAGAAGAAAACCTTCACCAAG TGGGTGAATTCGCACCTCGCTCGCGTGGGCTGCCACATCGGAGACCTGTATGCAGACCTGAGGGATGGCT TTGTCCTCACCCGGCTCCTGGAAGTGCTGTCGGGGGAGCAGCTGCCGAGACCCACTCGCGGCCGCATGCG GATCCACTCGCTGGAGAACGTGGACAAGGCTCTGCAGTTCCTGAAGGAGCAGCGCGTGCACCTGGAGAAC GTGGGGTCACATGACATCGTGGATGGGAACCACCGACTGACGCTGGGGCTGGTCTGGACCATCATCCTGC GCTTCCAGATTCAAGTCATCAAGATTGAGACAGAAGACAACAGAGAGACCCGCTCGGCCAAGGACGCTCT GCTCTTGTGGTGCCAGATGAAGACAGCTGGTTACCCTGAGGTAAACATCCAGAATTTCACTACTAGCTGG AGAGATGGCCTGGCCTTCAATGCCCTCATCCATCGCCACAGGCCTGATCTTGTGGACCTCAGCAAACTTA CCAAGTCCAACGCCAACTACAACCTGCAGAGAGCTTTCCGCACAGCCGAGCAGCACCTGGGGCTAGCACG TCTGCTGGACCCCGAAGACGTGAACATGGAGGCTCCAGATGAGAAGTCCATCATCACCTACGTGGTCTCC TTCTACCACTACTTCTCCAAGATGAAGGCTCTGGCTGTGGAGGGAAAACGGATTGGGAAGGTCCTGGACC AGGTGTTGGAGGTGGACAAGATCATAGAGCGCTACGAGGAGCTGGCGGCGGAGCTGCTGGCCTGGATCCA CCGCACCGTGGACCTCATCAGCAACCAAAAGTTTGCCAACTCACTGAGTGGGGTGCAGCAGCAGCTGCAG GCTTTCACTGCCTACTGTACCCTGGAGAAGCCGGTCAAGTTTCAGGAGAAGGGGAACCTGGAGGTGCTAC TCTTCAGCATCCAAAGCAAACTGCGCGCCCACAACCGGCGCCTCTTTGTGCCTCGGGAAGGCTGTGGCAT CTGGGATATTGACAAGGCATGGGGTGAGCTGGAGAAGGCAGAGCATGAGCGAGAGGCAGCCCTCAGAGCT GAGCTCATCCGACAGGAGAAGTTGGAACTCTTGGCTCAAAGATTTGACCACAAGGTGGCCATGAGGGAGA GCTGGCTAAATGAGAACCAGCGCCTGGTCTCTCAGGACAACTTCGGATATGAGCTGCCGGCAGTGGAGGC AGCCATGAAGAAGCACGAAGCCATCGAGGCTGACATCGCGGCCTACGAGGAGCGGGTTCAGGGTGTGGCG GAGCTAGCCCAGGCATTGGCAGCTGAAGGCTACTATGATGCCCGGCGAGTGGCAGCCCAGCGTGACAGCG TCCTCCGCCAGTGGGCCCTGCTGACTGGGCTGGTAGGTGCCCGGCGGACCCGGCTTGAGCAGAATCTGGC CCTGCAGAAGGTCTTCCAGGAGATGGTCTACATGGTGGACTGGATGGAGGAAATGCAGACTCAGCTGCTG TCTCGGGAATGTGGGCAGCACCTGGTGGAGGCTGATGACCTGCTGCAGAAGCATGGGCTACTGGAGGGCG ACATCGCAGCCCAGAGCGAGCGGGTGGAGGCCCTCAATGCTGCAGCCCTGAGATTCTCCCAGTTGCAGGG CTACCAACCCTGCGACCCTCAGGTCATTTGCAACCGTGTGAACCACGTGCACGGTTGTCTGTCGGAGCTG CAGGAGCAGGCGGCGCGGCGGCGCGCGGAGCTGGAAGCCTCTCGGAGCCTGTGGGCGCTGCTGCAGGAGC TGGAGGAGGCGGAGAGCTGGGCACGGGACAAGGAGCGCCTCCTAGAGGCCACGAGCGGCAGTGGTGGCGC GGCGGGCACGGCGGGAGGCGCGCACGACCTGTCCAGCACCGCTCGCCTGCTGGCGCAGCACAAGATCTTG CAGGGCGAACTGGGCGGCCGGCGGGCGCTACTGCAGCAGGCGCTGCGACGTGGAGAGGAGCTGGCTGCGG CGGGCGGCTCCGTGGGCCCCGGCGCCGAGCCCTTGCACTTGGCTGGTCTGGCGGAGCGCGCGGCTAGCGC TCGGCGCCGCTGGCAGCGACTGGAAGAGGCGGCTGCGCGCCGGGAACGGAGGCTGCAGGAGGCACGGGCG TTGCACCAGTTTGGCGCCGACCTGGACGGGCTCCTGGACTGGCTTCGCGACGCGTATCGTCTGGCAGCCG CCGGAGATTTCGGTCACGACGAAGCGTCCAGCCGTCGCCTGGCGCGCCAGCACCGAGCTCTCACCGGGGA GGTGGAAGCGCACCGTGGCCCTGTGGGTGGCCTGAGGCGTCAGCTGGCAACACTGGGAGGAGCCAGTGGC GCCGGGCCGCTGGTGGTGGCGCTGCAGGTGCGCGTGGTGGAAGCAGAACAGTTGTTCGCGGAGGTGACCG AGGTGGCCGCGCTGAGACGACAGTGGCTTCGGGACGCGCTCGCGGTGTACCGCATGTTTGGAGAGGTGCA TGCCTGCGAGTTGTGGATCGGAGAGAAAGAGCAGTGGCTGCTGGCCATGCGTGTGCCCGACTCCCTCGAC GACGTTGAGGTGGTGCAGCACCGATTTGAAAGTCTGGACCAGGAAATGAACAGCCTAATGGGCCGAGTCT TGGATGTGAACCAAACAGTTCAGGAGTTGGTGGAAGGAGGTCACCCCAGCTCCGATGAGGTGCGCTCCTG TCAGGACCATCTCAACAGCAGGTGGAACCGCATCGTGGAGCTGGTAGAACAGCGCAAAGAGGAGATGAGC GCCGTGCTGCTGGTGGAGAACCATGTACTGGAGGTGGCCGAGGTGCGCGCCCAGGTGCGAGAGAAGCGGA GGGCGGTGGAGAGCGCGCCCCGGGCGGGCGGAGCCCTGCAGTGGCGCCTGAGTGGCCTGGAGGCGGCCCT ACAGGCTCTGGAGCCGCGACAGGCCGCACTTCTGGAGGAGGCGGCGTTGTTGGCGGAACGCTTCCCGGCA CAGGCGACGCGCCTGCACCAGGGCGCCGAGGAGCTGGGAGCGGAGTGGGGTGCACTGGCTGGAGCGGCTC AGGCTTGCGGAGAGGCGGTGGCGGCGGCAGGTCGCCTGCAACGTTTCCTGCGCGATTTGGATACTTTCCT GGACTGGCTGGTGCGTGCGCAGGAGGCAGCGGGCGCCGTCGAGGGGCCTCTGCCGCGTAGCCTGGAGGAG GCGGACGGGCTGCTGGCGCGCCATGCCGCACTCAAGGAGGAGGTGGATCAGCGCGAGGAGGACTACGCGC GCATCGTAGCTGCCAGTGAGGCGTTGCTGGCGTCCGACGGTGCCGAGCTGGGCCCCGGTCTGGCGCTAGA CGAATGGCTGCCGCATCTCGAGGTTGGCTGGCACAAACTGCTCGGCTTGTGGGAGGAGCGCAGGGAGGCT CTGGTCCAGGCGCACGTCTATCAGCTCTTCCTGAGAGACCTGTGCCAGGCGCTCGCGGTGCTGCGCAACC AGGAGGTGGCGCTGTCTGGCGCTGAACTCCCTTGCACAGTGGAGTCGGTAGAGGAGGCCATGAAAAGGCA CCGGGATTTCCTCACCACGATGGAGCTGAACCAGCAAAAGATGCAGGTGGCGGTTCAAGCTGCAGAGGGC CTGCTGCGGCAAGGCAACGCCTACGGGGAGCAGGCGCAGGAGGCAGTGGCCCGGCTGCTAGAGAAGAGCC AAGAAAATCAGCTGAGGGCCCAGCAGTGGATGCAAAAACTCCTGGACCAGCTTGTGCTGCAGCACTTTCT TAGAGATTGTCATGAGCTGGATGGCTGGATCCACGAGAAAATGCTGATGGCTCGTGATGGCACCCGAGAA GACAGCCACAAGCTGCACAAGAGATGGCTCCGGCACCAGGCGTTCATGGCTGAGCTGGCTCAGAATAAGG AGTGGCTGGAGAAGATAGAAAGGGAGGGCCAGCAACTGATGCAGGAGAAACCGGAGCTGGCAGCTTCTGT GCGCAAGAAATTAGGTGAGATCCGGCAGTGCTGGGCAGAGCTGGAGAGCACCACACAGGCCAAGGCGCGC CAGCTCTTCGAGGCCAGCAAGGCGGACCAGCTGGTGCAGAGCTTCGCAGAGCTGGACAAGAGGCTGCTCC ACATGGAAAGCCAGTTGCAGGACGTAGACCCCGGTGGAGACCTGGCCACCGTCAACAGCCAGCTCAAGAA GTTACAGTCTATGGAGTCTCAGGTGGAGGAGTGGTGTCGAGAGGTGGGCGAGCTGCAGGCGCAGACAGCG GCGCTTCCACTGGAGCAGGCGAGCAAGGAGTTGGTGGGCGAGCGGCAGAGCGCGGTGGGAGAGCGCCTGG TGCGCCTGCTGGAGCCTTTGCAGGAGCGTCGCCGCTTGCTGCTGGCATCCAAGGAGCTGCATCAGGTGGC ACACGACCTGGATGACGAACTGGCGTGGGTTCAAGAGCGGCTGCCGCTGGCCATGCTGACAGAGCGAGGC ACTGGCTTGCAGGCTGTCCAGCAGCACATCAAAAAGAACCAGGGCCTGAGGCGGGAGATCCAGGCTCACG GGCCGCGCTTGGAGGAGGTGCTGGAGCGCGCGGGCGTGCTGGCCTCGTTGCGCAGCCCGGAGGCTGAGGC GGTGCGCCGCGGCCAGGAGCAGCTGCAGAGCGCCTGGACCGGACTGCGGGAAGCCACTGAACGGCGGCAG CAGACGCTGGACGCCGCCTTCCAGGTGGAACAGTACTACTTTGACGTGGCTGAGGTGGAGGCATGGCTGG GCGAGCAGGAGCTGCTCATGATGAGTGAGGACAAGGGCAAGGATGAACAAAGCACTCTGCAGCTGCTCAA GAAGCATCTGCAACTGGAGCAGGGAGTGGAGAACTATGAAGAAAGCATTGCCCAGCTGTCACGCCAGTGC CGGGCGCTACTGGAAATGGGACACCCAGACAGTGAGCAGATCAGCCGTCGACAGTCCCAGGTGGATCGCC TCTATGTGGCGCTCAAGGAGCTGGGTGAAGAGCGCAGGGTGAGCCTGGAACAGCAGTACTGGCTATACCA GCTCAGCCGCCAGGTGGACGAGCTGGAACACTGGATAGCCGAGAAGGAGGTGGTAGCTGGTTCACCAGAG CTGGGCCAGGACTTTGAACATGTGTCGGTGCTACAGGAGAAATTCTCAGAGTTCGCCAGTGAGACAGGAA CCGCAGGGCGGGAGCGGCTGGCAGCAGTAAACCAGATGGTGGACGAGCTGATTGAGTGTGGCCACACAGC AGCGGCCACCATGGCTGAGTGGAAGGACGGGCTGAACGAGGCCTGGGCTGAACTGCTGGAGCTCATGGGC ACTCGGGCCCAGCTGCTTGCTGCCTCTCGGGAGCTGCATAAGTTCTTCAGTGATGCCCGAGAGCTTCAAG GGCAGATCGAGGAGAAGCGGAGGAGGCTGCCCCGCCTGACGGCACCACCTGAGCCCAGACCCAGCGCTAG CTCCATGCAGCGGACCCTGCGAGCCTTTGAGCACGACCTGCAGCTCCTCGTGTCGCAGGTGCGGCAGCTG CAGGAAGGGGCGGCCCAGTTGCGGACTGTGTACGCGGGCGAGCACGCAGAGGCCATCGCCAGCCGTGAGC AGGAGGTGTTGCAGGGCTGGAAGGAGCTGCTGGCAGCCTGTGAGGATGCTCGTCTGCACGTCAGCTCCAC GGCTGACGCCCTGCGCTTCCACAGCCAGGCCCGAGACTTGCTCTCCTGGATGGACGGCATCGCGGGCCAG ATCGGGGCAGCTGACAAACCCAGGGATGTATCATCCGTGGAGGTGCTTATGAACTACCACCAGGGCCTGA AGACAGAATTGGAGGCTCGTGTGCCTGAGCTGACAGCCTGCCAGGAGCTGGGGCGTTCTCTATTACTCAA CAAAAGTGCCATGGCTGATGAGATCCAGGCACAGTTGGACAAGCTGGGAAGCCGGAAGGAGGAGGTGTCG GAGAAGTGGGACCGGCACTGGGAGTGGCTGCAACAGATGCTGGAGGTACACCAGTTTGCTCAGGAGGCCG TGGTGGCTGACGCCTGGCTGACAGCTCAGGAGCCGCTCCTGCAGAGCCGGGAGCTGGGCAGCAGTGTGGA TGAGGTGGAGCAGCTTATCCGGCGACACGAGGCCTTCCGAAAAGCGGCTGCGGCGTGGGAGGAGAGGTTC AGCTCTCTGCGGCGCTTGACCACGATCGAGAAACTCAAGGCAGAACAGAGTAAGCAGCCACCCACCCCAT TGCTGGGGCGCAAGTTCTTTGGGGACCCCACAGAGCTGGCGGCCAAGGCAGCACCCTTGCTGCGTCCAGG GGGCTATGACAGGGGCCTGGAGCCTTTGGCCCGCAGGGCCTCGGACACGCTGTCAGCGGAGGTGCGGACC CGGGTTGGTTATGTGCGCCAGGAGCTCAAGCCCGAGCGTCTCCAGCCGCGCATTGACCGGCTGCCGGAGA CCTCGGGGAAGGTAGAACCCGCGGCCCCAACAGCCGCAGCGTTGGACACGACGGACACCCCTGGAACTCC GGCGGTGACGGAGCAGGTCCGGCCCCGGCCGGAGCGCCAGGAGTTAGCGGATCGTGCGGAGGAGCTGCCA CGGAGACGAAGATCAGAACGCCAGGAGTCCACGGATCAACCCGAGGAGGCAGCGCGGAGGCGGCGGCCTG AGCGCCAGGAGTCCGCGGACCACGAGGGGCCTCACAGCCTTACTCTGGGTCGCTATGAGCAAATGGAGAG GCGGAGGGAGCGGCGGGAGCGGCGGATAGAACGACAGGAGTCTAGCGAGCAGGAGACTCCCACCAGAGGA GAGCTGGTCAAAGGGAAGGCCACCCTGGCTGACATCGTGGAGCAGCTTCAGGAGAAAGAGGCAGGCCCAG GGCTCCCTGCTGCGGTGCCGTCGCTGCCTCAGCCTCGGGAGCTTCCCCCTGGTCGCCTGCCCAACGGGCT TGAGCCGCCCGAGCGGACACCTCGGCCCGATCGGCCGCGGGCACGGGACCGGCCCAAGCCCCGCCGGCGG CCACGGCCTCGAGAGGGTGGTGAGGGCGGGGGAAGCCGGCGCTCGCGCTCCGCCCCTGCTCAGGGAGGCT CCGCCCCCGCGCCTCCACCTCCGCCCACTCACACAGTGCAGCACGAGGGCTTCCTGCTGCGCAAGCGCGA GCTCGACGCTAACCGCAAGTCGTCCAACCGGTCGTGGGTGAACCTGTACTGTGTGCTCAGCAAGGGGGAG CTGGGCTTCTACAAGGACTCCAAAGGCCCAGCATCAGGGGGCACGCATGGCGGGGAGCCACTGCTCAGCC TGCACAAAGCCACCAGCGAGGTGGCTAGTGACTACAAGAAAAAGAAGCATGTCTTCAAGCTCCAAACCCA GGATGGCAGCGAGTTCTTGCTCCAGGCTAAAGATGAGGAGGAGATGAACGGCTGGCTGGAGGCTGTGGCT AACTCCGTGGCAGAACACGCCGAGATCGCCCGATGGGGCCAGACACTACCTACCACATCATCCACAGACG AGGGCAACCCCAAGCGTGAGGGCGGGGAGCGTAGGGCCAGCGGGCGCCGGAAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001100845
Insert Size	7686 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100845.2, NP_001094315.2
RefSeq Size	8736 bp
RefSeq ORF	7686 bp
Locus ID	308458

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