Syngap1 (NM_001113409) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Syngap
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200180 representing NM_001113409 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTTAAGGCCCCCCACCCCGACCCCGTCAGGGGGCTCCGGTTCAGGTTCCTTGCCCCCTCCTTCCC ACCGCCAGCCTCTCCGCCGCCGCTGCTCTTCCTGCTGCTTTCCGGGGGAATACCACTTGGGTCGCTCGAG GAGGAAGAGTGTCCCCGGGGGGAAACAGTACAGCATGGAAGCCGCCCCCGCTGCGCCCTTCCGGCCCTCG CAAGGCTTCCTGAGCCGGAGGCTAAAAAGCTCCATCAAACGTACAAAGTCACAACCCAAACTTGACCGGA CCAGCAGCTTTCGACAGATCCTGCCTCGCTTCCGAAGTGCTGACCATGACCGGGCCCGGCTGATGCAGAG CTTCAAGGAGTCTCACTCCCATGAGTCCCTGCTGAGTCCCAGCAGTGCTGCTGAGGCCCTGGAGCTCAAC CTGGATGAAGACTCCATTATCAAGCCAGTACACAGCTCCATCCTGGGCCAGGAGTTCTGCTTTGAGGTAA CAACATCGTCTGGGACAAAATGTTTTGCCTGTCGGTCTGCAGCCGAAAGGGACAAATGGATTGAGAATCT ACAGAGGGCTGTGAAACCCAACAAGGACAACAGCCGCCGGGTAGATAACGTGCTGAAACTATGGATCATA GAAGCTCGAGAGCTGCCCCCCAAGAAGCGATATTACTGCGAGTTATGCCTGGACGACATGCTCTATGCAC GGACCACTTCCAAGCCCCGCTCAGCCTCAGGAGACACTGTCTTTTGGGGCGAGCACTTCGAGTTTAACAA CCTGCCTGCTGTCCGGGCGCTGCGGCTGCATCTGTACCGTGACTCGGACAAAAAGCGGAAGAAGGACAAG GCAGGCTACGTTGGCCTGGTGACTGTTCCAGTGGCCACCCTGGCTGGGCGCCACTTCACAGAGCAGTGGT ACCCCGTGACCCTGCCAACAGGAAGTGGGGGCTCTGGGGGTATGGGCTCGGGGGGAGGAGGGGGGTCAGG GGGCGGCTCAGGGGGCAAAGGGAAAGGAGGCTGTCCTGCTGTGCGGCTGAAGGCCCGTTACCAGACAATG AGTATCCTGCCCATGGAGCTATATAAGGAGTTTGCAGAATATGTGACCAACCACTACCGCATGCTGTGTG CCGTGCTGGAGCCCGCCCTCAATGTCAAGGGCAAGGAGGAGGTCGCTAGTGCACTGGTTCACATCCTGCA AAGCACAGGCAAGGCCAAGGACTTCCTTTCAGACATGGCCATGTCAGAGGTAGACCGGTTCATGGAGCGG GAACACCTCATATTCCGCGAGAACACGCTCGCCACTAAAGCCATAGAAGAGTATATGAGACTGATTGGCC AGAAATACCTCAAGGATGCCATTGGGGAGTTCATCCGGGCTCTGTATGAATCTGAGGAGAACTGTGAAGT AGACCCCATCAAGTGCACAGCGTCCAGTCTGGCAGAGCACCAGGCCAACCTGCGGATGTGCTGTGAGTTG GCCCTGTGCAAGGTGGTCAACTCCCATTGCGTGTTCCCGGGGGAGCTGAAGGAGGTGTTTGCATCATGGC GGCTGCGCTGTGCAGAGCGGGGCCGGGAGGACATTGCTGACAGGCTGATCAGCGCCTCGCTCTTCCTGCG CTTCCTCTGCCCGGCCATCATGTCGCCCAGTCTGTTTGGACTGATGCAGGAGTACCCAGATGAGCAGACC TCACGAACCCTCACCCTCATCGCCAAGGTTATCCAGAACCTGGCCAACTTTTCCAAGTTTACCTCAAAGG AGGACTTCCTGGGCTTCATGAACGAGTTTCTGGAGCTGGAGTGGGGTTCTATGCAGCAATTCTTGTATGA GATATCCAACCTGGACACACTGACCAACAGCAGCAGTTTTGAGGGCTACATAGACTTGGGCCGCGAGCTC TCCACACTTCACGCCCTGCTCTGGGAGGTGCTGCCCCAGCTCAGCAAGGAAGCCCTCCTGAAGCTGGGCC CGCTGCCCCGGCTCCTCAGCGACATCAGCACAGCCCTGAGGAACCCTAACATCCAAAGGCAGCCGAGCCG CCAGAGCGAGCGCGCTCGGTCTCAGCCCATGGTGCTGCGCGGGCCGTCAGCCGAGATGCAGGGCTACATG ATGCGGGACCTCAACAGCTCCATCGACCTTCAGTCCTTCATGGCTCGAGGCCTCAACAGCTCTATGGACA TGGCTCGCCTCCCCTCCCCAACCAAGGAGAAACCCCCGCCGCCCCCTCCCGGTGGGGGTAAAGACCTGTT CTATGTGAGCCGGCCACCACTGGCCCGGTCCTCCCCAGCATACTGCACGAGCAGCTCGGACATCACAGAG CCGGAGCAGAAGATGCTGAGTGTCAACAAGAGTGTGTCCATGCTGGACCTGCAGGGCGACGGGCCTGGGG GCCGCCTTAACAGCAGTAGTGTTTCCAACCTGGCAGCTGTTGGGGACCTGTTGCACTCAAGCCAGGCTTC ACTGACAGCAGCCTTGGGGTTGCGGCCTGCACCTGCCGGGCGCCTCTCCCAAGGGAGTGGCTCTTCCATC ACAGCAGCCGGCATGCGCCTCAGCCAGATGGGTGTCACTACGGATGGTGTCCCCGCCCAGCAACTGCGCA TCCCTCTTTCCTTCCAGAACCCTCTCTTCCATATGGCTGCCGATGGACCAGGGCCCCCAGCAGGCCATGG AGGGAGCAGTGGCCATGGTCCACCTTCCTCCCATCACCACCACCACCACCATCACCATCACCGAGGGGGA GAACCCCCAGGGGACACTTTTGCCCCGTTCCATGGCTATAGCAAGAGCGAGGACCTCTCTACAGGGGTCC CTAAGCCCCCTGCGGCCTCCATCCTTCACAGCCACAGCTACAGTGATGAGTTTGGACCCTCTGGTACTGA TTTTACCCGTCGGCAGCTCTCACTTCAGGACAACCTACAGCACATGCTCTCCCCGCCCCAGATCACCATC GGTCCCCAGAGGCCAGCTCCCTCAGGGCCAGGAGGGGGCAGTGGTGGGGGCAGTGGTGGGGGCGGTGGGG GCCAGCCACCTCCCTTGCAGAGGGGCAAATCTCAGCAGTTGACAGTGAGTGCTGCCCAGAAACCCCGGCC GTCCAGCGGGAACCTATTGCAGTCCCCGGAACCAAGTTATGGTCCTGCCCGTCCACGGCAACAGAGCCTC AGCAAAGAGGGCAGCATTGGGGGCAGCGGGGGCAGCGGTGGCGGAGGGGGTGGGGGGCTCAAGCCCTCCA TCACCAAGCAGCATTCCCAGACTCCATCCACGCTGAACCCCACGATGCCGGCCTCGGAGCGGACTGTAGC CTGGGTGTCCAATATGCCTCACCTGTCCGCTGACATCGAGAGTGCACACATTGAGCGGGAAGAGTACAAG CTGAAGGAGTACTCGAAGTCCATGGACGAGAGCCGACTGGACAGGGTGAAGGAGTACGAGGAGGAGATCC ACTCACTGAAGGAAAGGCTACACATGTCCAACCGGAAGCTGGAAGAGTACGAGCGGAGGCTGCTGTCCCA GGAAGAGCAGACCAGCAAGATCCTGATGCAGTACCAAGCCCGCCTGGAGCAGAGCGAGAAGCGCTTGAGG CAGCAGCAGGTGGAGAAGGACTCCCAGATCAAGAGCATCATTGGCAGGCTGATGCTGGTGGAGGAGGAGC TGCGCCGGGACCACCCCGCCATGGCTGAGCCGCTGCCTGAACCCAAGAAGAGGCTGCTCGACGCTCAGAG AGGCAGCTTCCCCCCTTGGGTCCAACAAACCCGCGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200180 representing NM_001113409 Red=Cloning site Green=Tags(s) MGLRPPTPTPSGGSGSGSLPPPSHRQPLRRRCSSCCFPGEYHLGRSRRKSVPGGKQYSMEAAPAAPFRPS QGFLSRRLKSSIKRTKSQPKLDRTSSFRQILPRFRSADHDRARLMQSFKESHSHESLLSPSSAAEALELN LDEDSIIKPVHSSILGQEFCFEVTTSSGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWII EARELPPKKRYYCELCLDDMLYARTTSKPRSASGDTVFWGEHFEFNNLPAVRALRLHLYRDSDKKRKKDK AGYVGLVTVPVATLAGRHFTEQWYPVTLPTGSGGSGGMGSGGGGGSGGGSGGKGKGGCPAVRLKARYQTM SILPMELYKEFAEYVTNHYRMLCAVLEPALNVKGKEEVASALVHILQSTGKAKDFLSDMAMSEVDRFMER EHLIFRENTLATKAIEEYMRLIGQKYLKDAIGEFIRALYESEENCEVDPIKCTASSLAEHQANLRMCCEL ALCKVVNSHCVFPGELKEVFASWRLRCAERGREDIADRLISASLFLRFLCPAIMSPSLFGLMQEYPDEQT SRTLTLIAKVIQNLANFSKFTSKEDFLGFMNEFLELEWGSMQQFLYEISNLDTLTNSSSFEGYIDLGREL STLHALLWEVLPQLSKEALLKLGPLPRLLSDISTALRNPNIQRQPSRQSERARSQPMVLRGPSAEMQGYM MRDLNSSIDLQSFMARGLNSSMDMARLPSPTKEKPPPPPPGGGKDLFYVSRPPLARSSPAYCTSSSDITE PEQKMLSVNKSVSMLDLQGDGPGGRLNSSSVSNLAAVGDLLHSSQASLTAALGLRPAPAGRLSQGSGSSI TAAGMRLSQMGVTTDGVPAQQLRIPLSFQNPLFHMAADGPGPPAGHGGSSGHGPPSSHHHHHHHHHHRGG EPPGDTFAPFHGYSKSEDLSTGVPKPPAASILHSHSYSDEFGPSGTDFTRRQLSLQDNLQHMLSPPQITI GPQRPAPSGPGGGSGGGSGGGGGGQPPPLQRGKSQQLTVSAAQKPRPSSGNLLQSPEPSYGPARPRQQSL SKEGSIGGSGGSGGGGGGGLKPSITKQHSQTPSTLNPTMPASERTVAWVSNMPHLSADIESAHIEREEYK LKEYSKSMDESRLDRVKEYEEEIHSLKERLHMSNRKLEEYERRLLSQEEQTSKILMQYQARLEQSEKRLR QQQVEKDSQIKSIIGRLMLVEEELRRDHPAMAEPLPEPKKRLLDAQRGSFPPWVQQTRV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001113409
ORF Size	3747 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001113409.3
RefSeq Size	5805 bp
RefSeq ORF	3750 bp
Locus ID	192117
MW	137.3 kDa
Gene Summary	This gene encodes a Ras GTPase activating protein that is a member of the N-methyl-D-aspartate receptor complex. The N-terminal domain of the protein contains a Ras-GAP domain, a pleckstrin homology domain, and a C2 domain that may be involved in binding of calcium and phospholipids. The C-terminal domain consists of a ten histidine repeat region, serine and tyrosine phosphorylation sites, and a T/SXV motif required for postsynaptic scaffold protein interaction. The encoded protein negatively regulates Ras, Rap and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor trafficking to the postsynaptic membrane to regulate synaptic plasticity and neuronal homeostasis. Homozygous null mutations in mice result in early post-embryonic lethality, while heterozygous mutant mice display a variety of phenotypes that include learning and memory defects, hyperactivity, and audiogenic seizures. [provided by RefSeq, Nov 2016]

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