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Actn4 (NM_031675) Rat Tagged ORF Clone
CAT＃: RR202011
- TrueORF®
Actn4 (Myc-DDK-tagged ORF) - Rat actinin alpha 4 (Actn4), (10 ug)
"NM_031675" in other vectors (3)
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CNY 11,780.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202011 representing NM_031675
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGACTATCACGCGGCGAACCAGGCGTACCAGTACGGCCCAAGCAGCGGCGGCAATGGCACGGGCG GCGGCGGAGGCATGGGCGACTACATGGCCCAGGAGGATGACTGGGACCGGGACCTGCTGTTGGACCCGGC CTGGGAGAAGCAGCAGCGCAAGACCTTCACAGCCTGGTGCAACTCCCATCTGCGGAAGGCTGGCACTCAG ATCGAGAACATCGATGAGGATTTCCGGGATGGGCTCAAACTTATGCTTCTGCTGGAGGTCATTTCAGGGG AGCGGTTGCCTAAGCCAGAGCGGGGGAAGATGCGAGTGCACAAGATCAACAATGTAAACAAAGCCCTGGA CTTCATCGCCAGCAAGGGAGTCAAGCTGGTGTCCATTGGGGCAGAAGAGATCGTGGATGGCAATGCAAAG ATGACCCTGGGAATGATCTGGACCATCATCCTCAGGTTCGCCATCCAAGACATCTCTGTGGAAGAGACCT CTGCCAAGGAAGGACTCCTCCTCTGGTGCCAGAGAAAGACAGCCCCGTATAAAAACGTCAACGTCCAGAA CTTCCATATCAGCTGGAAGGATGGTCTGGCCTTCAACGCGCTCATCCACCGGCACAGACCTGAGCTGATT GAGTATGACAAACTGAGGAAGGATGATCCAGTCACCAACCTAAACAATGCATTTGAAGTGGCTGAGAAAT ACCTTGATATCCCCAAGATGTTGGATGCTGAGGACATCGTGAACACAGCCCGGCCCGACGAGAAGGCCAT AATGACATATGTGTCCAGCTTCTACCATGCCTTTTCAGGAGCGCAGAAGGCTGAGACTGCTGCCAACCGG ATCTGCAAGGTGCTGGCTGTGAACCAGGAAAATGAGCACCTGATGGAAGATTACGAGCGCCTGGCCAGTG ATCTTCTAGAGTGGATCCGGCGCACCATCCCCTGGCTGGAAGACCGTGTGCCTCAGAAGACCATCCAGGA GATGCAGCAGAAGCTGGAGGACTTCCGAGACTATAGGCGTGTGCACAAGCCCCCCAAGGTACAGGAGAAG TGTCAGCTGGAGATCAACTTCAACACCCTGCAGACCAAGCTGCGGCTGAGCAACCGGCCTGCCTTCATGC CCTCCGAGGGCAGGATGGTCTCGGACATCAACAATGGGTGGCAACATCTGGAGCAGGCTGAGAAGGGCTA TGAAGAATGGCTCTTGAATGAAATCCGTAGGCTGGAACGACTTGATCACCTGGCAGAGAAATTCCGGCAG AAGGCTTCCATCCATGAGGCCTGGACCGATGGGAAGGAGGCCATGCTGAAGCACCGGGACTATGAGACAG CCACCCTGTCAGACATCAAAGCTCTGATCCGAAAGCATGAGGCCTTTGAGAGTGACCTGGCGGCGCACCA GGACCGCGTGGAGCAGATCGCGGCTATTGCCCAGGAGCTCAACGAACTGGACTACTATGACTCCCACAAC GTCAACACACGGTGCCAGAAGATCTGTGACCAGTGGGATAACCTGGGCTCTCTGACCCACAGTCGCAGGG AAGCCTTGGAGAAAACAGAGAAACAGCTGGAGACCATCGACCAGCTACACTTGGAGTATGCTAAGCGGGC CGCGCCCTTCAACAACTGGATGGAGAGTGCCATGGAGGACCTGCAGGACATGTTCATCGTCCACACCATC GAGGAGATTGAGGGCCTGATCTCAGCCCACGACCAGTTCAAGTCCACCCTGCCAGATGCCGACAGGGAGC GTGAGGCCATCCTGGCCATCCACAAAGAGGCCCAAAGGATCGCTGAGAGCAATCACATCAAGCTGTCGGG CAGCAACCCCTACACCAGCGTTACACCCCAGATCATCAACTCCAAATGGGAGAAGGTGCAGCAGCTGGTG CCGAAGCGGGACCACGCACTCCTGGAAGAGCAGAGCAAGCAGCAATCCAATGAGCACCTTCGCCGACAGT TTGCCAGCCAAGCCAATATGGTGGGGCCCTGGATCCAGACCAAGATGGAGGAGATTGGGCGCATCTCCAT CGAGATGAACGGGACTCTGGAAGACCAGCTGAGTCACTTGAAGCAGTACGAGCGCAGCATTGTGGACTAC AAGCCCAACCTGGACTTGCTGGAGCAGCAGCACCAGCTCATCCAGGAGGCCCTCATCTTCGACAACAAGC ACACCAACTACACAATGGAGCACCTCCGTGTGGGCTGGGAGCAGCTGCTCACCACCATTGCCCGCACCAT CAACGAGGTAGAGAATCAGATCCTCACCCGAGATGCCAAGGGCATCAGCCAGGAGCAAATGCAGGAGTTC CGAGCCTCCTTCAACCACTTTGACAAGGACCATGGCGGGGCACTGGGGCCTGAGGAATTCAAGGCCTGCC TCATCAGCCTGGGCTACGACGTGGAGAATGACCGGCAGGGTGATGCTGAGTTCAACCGGATCATGAGTGT GGTCGACCCCAACCATAGTGGCCTCGTGACCTTCCAAGCCTTCATTGACTTCATGTCAAGGGAGACCACA GACACAGACACCGCCGATCAGGTCATCGCCTCCTTCAAGGTCCTGGCCGGGGACAAGAACTTCATCACTG CTGAGGAACTTCGGAGAGAGCTGCCCCCTGACCAGGCTGAGTACTGCATCGCCCGCATGGCGCCCTACCA GGGCCCTGATGCTGCTCCAGGCGCCCTTGACTACAAGTCCTTCTCTACAGCCCTCTATGGGGAGAGCGAC CTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202011 representing NM_031675
Red=Cloning site Green=Tags(s) MVDYHAANQAYQYGPSSGGNGTGGGGGMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQ IENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAK MTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELI EYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANR ICKVLAVNQENEHLMEDYERLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEK CQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQ KASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHN VNTRCQKICDQWDNLGSLTHSRREALEKTEKQLETIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTI EEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTSVTPQIINSKWEKVQQLV PKRDHALLEEQSKQQSNEHLRRQFASQANMVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDY KPNLDLLEQQHQLIQEALIFDNKHTNYTMEHLRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEF RASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGDAEFNRIMSVVDPNHSGLVTFQAFIDFMSRETT DTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAAPGALDYKSFSTALYGESD L TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031675 |
| ORF Size | 2733 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031675.2, NP_113863.2 |
| RefSeq Size | 3885 bp |
| RefSeq ORF | 2736 bp |
| Locus ID | 63836 |
| UniProt ID | Q9QXQ0 |
| MW | 104.9 kDa |
| Gene Summary | alpha-actinin cytoskeletal protein that anchors various actin-containing microfilaments to cellular structures; anchors V myosins to cellular structures via the RING finger-containing protein BERP [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202011 | Actn4 (untagged ORF) - Rat actinin alpha 4 (Actn4), (10 ug) |
CNY 11,780.00 |
|
| RR202011L3 | Lenti ORF clone of Actn4 (Myc-DDK-tagged ORF) - Rat actinin alpha 4 (Actn4), (10 ug) |
CNY 13,400.00 |
|
| RR202011L4 | Lenti ORF clone of Actn4 (mGFP-tagged ORF) - Rat actinin alpha 4 (Actn4), (10 ug) |
CNY 14,820.00 |
