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Nasp (NM_001005543) Rat Tagged ORF Clone
CAT＃: RR202493
- TrueORF®
Nasp (Myc-DDK-tagged ORF) - Rat nuclear autoantigenic sperm protein (histone-binding) (Nasp), (10 ug)
"NM_001005543" in other vectors (3)
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CNY 9,980.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC93869 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202493 representing NM_001005543
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACAGAGTCTACAGCCACTGCTGCCATCGCCGCGGAACTGGTTTCCGCTGACAAAGTTGAAGATG CTCCTGCTCCTTCTACCTCTGCAGATAAAATGGAGAGTCTGGATGTGGATAGTGAAGCTAAGAAACTACT GGGATTAGGACAGAAACATCTGGTGATGGGTGACATTCCAGCAGCTGTTAATGCATTCCAAGAAGCAGCT AGTCTTTTAGGTAAGAAGTATGGAGAAACGGCTAATGAGTGTGGAGAAGCCTTCTTTTTTTATGGGAAGT CACTTCTGGAGTTGGCCAGAATGGAGAATGGAGTGTTGGGAAATGCCTTAGAAGGAGTGCATGTAGAAGA GGAAGAAGGAGAAAAAACAGAAGATGAATCTTTGGTAGAAAATAATGATAATGTAGATGAGGAAGCAAGG GAAGAGTTGAGAGAACAGGTTTATGACGCCATGGGAGAAAAAGAAGCCAAAAAAGCAGAAGGCCAGTCTC TGACAAAGCCTGAAACTGATAAAGAACAGGAAAGTGAAGTGGAGAAGGGTGGAAGAGAAGATATGGATAT AAGTGAGCCTGCAGAGAAGCTCCAGGAAAAAGTTGAATCGACTTCAAAGCAGTTAACTGAATCCTCTGAA GAGGCAAAAGAAGCAGCAATACCAGGACTGAATGAAGATGAGGTCACTTCTGGGAAGACAGAACAGGAAT CATTGTGTACTGAGGAAGGAAAATCCATTTCTGGAGTTTATGTTCAAAATAAAGAATTCAGAGAAGCAGT GCCTCAGGAGGAGGGAGAGGAAATGATAAGCTTAGAAAAAAAGCCAAAAGAAACTTCAGAAGATCAGACT ATTGGGGCTCCAGAAAAGCAGGACACTTTAATGAAGGTGGTAGAAATAGAAGCTGAAATAGACTCTGAAG TCAAGTCAGTGGATGTGGGTGGGGAGGAGCCGAAGGATCAGGGAGCTATCTCTGAGAGTGAACTAGGAAA GGCTGTTCTTATGCAACTCTCAGGGCAAGATGTTGAAGTATCACCAGTAGTGGCTGCAGAGGCTGGATCA GAAGTCTCTGAGAAGCCAGGGCAGGAGATTACAGTTCTTCCCAACAATGGTCCAGTTGTTGGACAATCAT CTGCAGGAGATCAAACTCCTAGTGAACCACAGAATTCTGCAGAAAGACTGTCAGAAACTAAAGATGGCGC AAGTGTAGAGGAGGTCAAGGCAGAGCTGGTTCCTGAACAGGAGGAAGCTATGCCTCCTGTAGAAGAGTCT GAGGCAGCTGGAGATGGGGTTGAGACCAAGGTAGCCCAGAGGGCCACGGAGAAAGCACCTGAAGACAAAT TTAAGATAGCTGCTAATGAAGAGACACAAGAGAGAGACGAACAGATGAAAGAGGGTGAAGAAACTGAAGG CTCAGAGGAAGAAGACAAAGAAAATGACAAGGCTGAAGAAACAACAAATGAATCAGTTCTTGAAAAAAAG ACTCTTCAAGAAAATGAAGAAGAGGAGATTGGGAATTTAGAACTTGCCTGGGATATGCTAGATTTAGCCA AGATTATTTTTAAGAGGCAAGAAACAAAAGAAGCCCAGCTTTATGCTGCACAAGCCCATCTTAAACTTGG AGAAGTTAGTGTTGAATCTGAGAATTATATCCAAGCTGTGGAGGAGTTTCAGGCTTGCCTAAGCCTGCAA GAGCAGTATTTGGAAGCTCACGATCGTCTCCTTGCGGAGACTCACTACCAGTTGGGTTTGGCCTATGGTT ACAACTCTCAGTATGATGAGGCTGTAGCACAGTTTGGCAAATCTATTGACGTCATTGAGAAGAGAATGGC TGTACTACTTGAGCAGATGAAGGAGGCTGAAGGATCATTTACTGAATACGAGAAAGAAATCGAGGAGCTG AAAGAACTGCTCCCCGAGATTCGGGAGAAGATAGAGGATGCAAAGGAGTCTCAGCGGAGTGGGAATGTAG CTGAGCTGGCTCTGAAGGCCACTCTGGTCGAGAGCTCTACTTCAGGTTTCACTCCTAGTGGAGCTGGTGC CTCGGTATCCATGATTGCCAGTAGAAAACCAACAGATGGCGCTTCCTCATCAAACTGTGTCACTGATATT TCTCATCTTGTCAGAAAGAAGAGGAAACCAGAGGAAGAGAGTCCCCGGAAAGATGATGCAAAGAAAGCTA AACCAGAACCAGAGGTGAATGGAGGCAGTGGAGATGCTGTCTCCAGTGGAAATGAAGTTTCAGAAAACAT GGAGGCAGAGGCTGAGAATCAGGCTGAGAGCCAGACAACAGCAGAAGGGACAGTGGACTCTGCAGCTACA GTTAAAAGCACTGCATGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202493 representing NM_001005543
Red=Cloning site Green=Tags(s) MATESTATAAIAAELVSADKVEDAPAPSTSADKMESLDVDSEAKKLLGLGQKHLVMGDIPAAVNAFQEAA SLLGKKYGETANECGEAFFFYGKSLLELARMENGVLGNALEGVHVEEEEGEKTEDESLVENNDNVDEEAR EELREQVYDAMGEKEAKKAEGQSLTKPETDKEQESEVEKGGREDMDISEPAEKLQEKVESTSKQLTESSE EAKEAAIPGLNEDEVTSGKTEQESLCTEEGKSISGVYVQNKEFREAVPQEEGEEMISLEKKPKETSEDQT IGAPEKQDTLMKVVEIEAEIDSEVKSVDVGGEEPKDQGAISESELGKAVLMQLSGQDVEVSPVVAAEAGS EVSEKPGQEITVLPNNGPVVGQSSAGDQTPSEPQNSAERLSETKDGASVEEVKAELVPEQEEAMPPVEES EAAGDGVETKVAQRATEKAPEDKFKIAANEETQERDEQMKEGEETEGSEEEDKENDKAEETTNESVLEKK TLQENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLGEVSVESENYIQAVEEFQACLSLQ EQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFGKSIDVIEKRMAVLLEQMKEAEGSFTEYEKEIEEL KELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPSGAGASVSMIASRKPTDGASSSNCVTDI SHLVRKKRKPEEESPRKDDAKKAKPEPEVNGGSGDAVSSGNEVSENMEAEAENQAESQTTAEGTVDSAAT VKSTAC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001005543 |
| ORF Size | 2328 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001005543.1, NP_001005543.1 |
| RefSeq Size | 2627 bp |
| RefSeq ORF | 2331 bp |
| Locus ID | 298441 |
| UniProt ID | Q66HD3 |
| MW | 84.2 kDa |
| Gene Summary | Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202493 | Nasp (untagged ORF) - Rat nuclear autoantigenic sperm protein (histone-binding) (Nasp), (10 ug) |
CNY 9,980.00 |
|
| RR202493L3 | Lenti ORF clone of Nasp (Myc-DDK-tagged ORF) - Rat nuclear autoantigenic sperm protein (histone-binding) (Nasp), (10 ug) |
CNY 11,780.00 |
|
| RR202493L4 | Lenti ORF clone of Nasp (mGFP-tagged ORF) - Rat nuclear autoantigenic sperm protein (histone-binding) (Nasp), (10 ug) |
CNY 12,920.00 |
