Cacna1i (NM_020084) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203004 representing NM_020084 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACAGCAACTTACCGCCCTCATCTGCAGCAGCCCCGGCCCCTGAGCCGGGAATCACTGAGCAGC CGGGGCCCCGGAGTCCCCCTCCATCCCCTCCAGGCCTGGAGGAGCCATTGGAAGGAACCAACCCTGACGT CCCACATCCAGACCTGGCTCCTGTTGCTTTCTTCTGCCTGCGCCAGACCACGAGCCCACGGAACTGGTGC ATCAAGATGGTTTGTAACCCGTGGTTCGAGTGTGTGAGCATGCTGGTTATTCTGCTGAACTGTGTGACCC TGGGCATGTACCAGCCATGTGATGACATGGAGTGCCTGTCGGACCGTTGCAAGATCCTGCAGGTCTTCGA TGACTTCATCTTCATCTTCTTTGCCATGGAGATGGTGCTTAAGATGGTGGCCCTGGGCATTTTTGGCAAG AAGTGCTACCTCGGAGACACATGGAACCGCCTGGATTTCTTCATTGTCATGGCAGGGATGGTTGAGTACT CTCTGGACCTACAGAACATCAACCTGTCAGCCATCCGCACTGTGCGTGTCCTGAGGCCTCTCAAAGCCAT CAACCGTGTACCCAGCATGCGGATCCTGGTGAACCTGCTGCTCGACACGCTGCCCATGCTGGGGAACGTG CTCCTGCTCTGTTTCTTCGTCTTCTTCATCTTCGGCATCATTGGCGTGCAGCTCTGGGCAGGCCTGCTAC GGAACCGCTGCTTCCTGGAAGAGAACTTCACCATACAAGGGGATGTGGCCCTGCCCCCTTATTACCAACC AGAGGAGGATGACGAGATGCCCTTTATCTGCTCCCTGACTGGGGACAATGGCATCATGGGCTGCCACGAG ATCCCCCCACTGAAGGAGCAGGGCCGGGAATGCTGCCTGTCCAAAGATGATGTGTATGACTTCGGGGCGG GGCGCCAGGACCTCAACGCCAGCGGTCTGTGCGTCAACTGGAACCGCTACTACAACGTCTGCCGCACGGG CAACGCCAACCCTCACAAGGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATTGTGATTTTCCAG GTGATCACTCTGGAAGGCTGGGTGGAGATCATGTACTATGTGATGGACGCACATTCTTTCTACAACTTCA TCTACTTCATTCTGCTCATCATAGTGGGCTCCTTCTTCATGATCAACTTGTGCCTCGTTGTCATAGCAAC CCAGTTCTCTGAGACCAAGCAACGGGAGCACCGGCTGATGCTGGAGCAACGCCAGCGCTACCTGTCCTCC AGCACGGTGGCCAGTTACGCTGAGCCCGGTGATTGCTATGAGGAGATCTTCCAATATGTCTGTCACATCC TTCGCAAAGCCAAGCGCCGTGCCCTAGGCCTCTACCAGGCCCTGCAGAACCGGCGCCAGGCCATGGGCCC GGGGACACCAGCCCCTGCCAAGCCTGGGCCCCATGCCAAGGAGCCCAGCCACTGCAAGCTGTGCCCACGA CACAGCCCCCTGGACCCCACTCCCCACACACTGGTGCAGCCCATCTCTGCCATTCTGGCCTCTGACCCCA GCAGCTGCCCTCACTGCCAGCACGAGGCAGGCAGGCGGCCCTCTGGCCTGGGCAGCACTGACTCAGGCCA GGAAGGCTCAGGTTCTGGTGGCTCTGCAGAGGCCGAAGCCAATGGGGATGGACCCCAGAGCAGTGAGGAT GGGGTCTCCTCGGACCTGGGGAAGGAGGAGGAACAGGAGGACGGGGCAGCCCGACTGTGTGGGGATGTGT GGCGCGAGACACGAGAAAAGCTGCGGGGCATCGTGGACAGCAAGTACTTCAACAGAGGTATCATGATGGC TATCCTGGTGAACACAGTCAGCATGGGCATCGAGCACCACGAACAGCCCGAGGAGCTGACCAACATCCTG GAGATCTGCAATGTGGTCTTCACCAGTATGTTTGCCCTGGAGATGATCCTGAAACTGGCCGCCTTTGGGC TCTTCGACTACCTGCGGAACCCTTACAACATCTTTGACAGCATCATCGTCATCATCAGCATCTGGGAAAT CGTGGGGCAGGCAGACGGTGGCCTGTCTGTGCTGCGCACCTTCCGGTTGCTGCGGGTGCTGAAGCTGGTG CGCTTCATGCCGGCGCTGCGGCGCCAGCTCGTGGTGCTCATGAAGACCATGGACAACGTGGCCACCTTCT GCATGCTACTCATGCTGTTCATCTTCATCTTCAGCATCCTTGGGATGCATATCTTTGGCTGCAAATTCAG CCTCCGCACGGACACGGGAGACACCGTTCCTGACAGGAAGAACTTCGATTCCTTACTGTGGGCCATCGTC ACAGTGTTCCAGATCCTCACTCAGGAGGACTGGAACGTTGTCCTGTACAATGGCATGGCCTCCACCACCC CCTGGGCCTCCCTCTATTTTGTTGCCCTCATGACCTTTGGCAACTACGTTCTCTTCAATCTCCTGGTGGC TATCCTGGTAGAGGGTTTCCAGGCTGAGGGTGATGCTAATCGTTCCTACTCTGATGAGGACCAGAGCTCA TCCAATTTGGAGGAGTTTGACAAGCTCCCAGAGGGCCTGGACAACAGTAGAGATCTCAAGCTCTGCCCAA TACCCATGACACCCAATGGACACCTGGACCCTAGCCTCCCTCTGGGTGCGCATCTGGGTCCTGCTGGTAC CATGGGTACTGCCCCCCGCCTCTCACTGCAGCCAGACCCGGTACTGGTGGCCCTAGACTCTCGGAAAAGC AGTGTCATGTCCCTGGGCAGGATGAGCTATGATCAGCGATCCTTGTCCAGCTCCCGGAGCTCCTACTACG GGCCCTGGGGCCGCAGTGGGACCTGGGCTAGCCGCCGCTCCAGCTGGAACAGCCTGAAACACAAGCCGCC CTCAGCTGAGCATGAGTCCTTACTGTCTGGGGAGGGTGGAGGTAGCTGCGTCAGGGCCTGTGAAGGCGCC CGGGAGGAGGCGCCAACTCGCACCGCACCCCTGCATGCTCCACACGCGCACCACGCGCACCATGGACCCC ACCTGGCACACCGTCACCGACACCACCGCCGGACTCTGTCCCTTGATACCAGGGACTCTGTTGACCTGGG AGAGCTGGTGCCCGTGGTGGGTGCCCACTCACGGGCCGCTTGGAGGGGGGCGGGTCAGGCCCCTGGGCAC GAGGACTGCAATGGCAGAATGCCCAACATAGCCAAGGATGTCTTCACCAAGATGGATGACTGCCGCGACC GCGGGGAGGACGAGGAGGAGATCGACTATACCCTGTGTTTCCGGGTCCGCAAGATGATTGATGTGTACAA GCCGGACTGGTGCGAAGTCCGCGAGGACTGGTCGGTCTACCTCTTCTCCCCCGAGAACAAGTTCCGGATC CTGTGTCAGACTATCATTGCTCACAAGCTTTTTGACTACGTGGTCTTGGCCTTTATCTTCCTCAACTGTA TCACCATTGCTCTGGAGAGACCCCAGATTGAAGCTGGTAGCACTGAGCGCATCTTCCTCACGGTGTCTAA CTACATCTTCACAGCCATCTTCGTGGGCGAGATGACACTGAAGGTGGTTTCTCTGGGCCTGTACTTTGGT GAGCAGGCGTACCTGCGTAGCAGCTGGAATGTACTGGATGGTTTCCTGGTCTTTGTGTCCATCATCGATA TCGTAGTGTCCGTGGCCTCTGCTGGGGGAGCCAAGATTCTGGGGGTCCTCCGGGTCCTGCGGCTCCTGCG TACCTTACGTCCTTTGAGGGTTATCAGCCGGGCCCCTGGGCTGAAGCTGGTGGTAGAGACGCTCATCTCC TCCCTCAAGCCCATTGGGAACATCGTCCTCATCTGCTGTGCCTTCTTCATCATCTTCGGCATCCTGGGGG TGCAGCTTTTCAAAGGCAAGTTCTACCATTGTTTGGGAGTGGACACCCGAAACATCACCAACCGATCTGA CTGCGTGGCGGCCAACTACCGCTGGGTGCATCACAAATACAACTTTGACAACCTGGGCCAGGCATTGATG TCCCTCTTTGTCTTGGCCTCCAAGGACGGCTGGGTGAACATCATGTATAATGGATTAGATGCTGTTGCTG TGGACCAGCAGCCAGTGACGAACCACAACCCCTGGATGCTACTGTACTTCATTTCGTTCCTGCTCATCGT CAGCTTCTTTGTGCTCAACATGTTTGTGGGCGTGGTCGTGGAGAACTTCCACAAGTGCCGGCAGCACCAG GAGGCTGAGGAGGCGCGGAGGCGTGAGGAGAAACGGCTGCGGCGCCTGGAAAAGAAGCGCCGTAAGGCTC AGAGGCTGCCCTACTATGCTACCTACTGTCCCACAAGGCTGCTCATCCACTCCATGTGCACCAGCCACTA CCTGGACATCTTCATTACCTTCATCATCTGCCTCAATGTTGTCACCATGTCCCTGGAGCACTACAACCAG CCTACATCCCTAGAGACAGCCCTTAAGTACTGCAACTACATGTTCACCACTGTCTTTGTGCTGGAGGCTG TGCTGAAGCTGGTGGCATTTGGCCTGAGGCGTTTCTTCAAGGACCGATGGAACCAGCTGGACCTGGCCAT TGTGCTGCTGTCCGTCATGGGCATCACACTGGAGGAGATCGAGATCAATGCCGCCCTTCCCATCAACCCC ACCATCATCCGTATCATGCGTGTTCTGCGTATCGCCCGGGTGTTGAAGCTATTGAAGATGGCCACAGGAA TGCGGGCCCTGCTGGACACAGTGGTACAGGCTCTGCCCCAGGTGGGCAACCTGGGCCTGCTCTTCATGCT GCTCTTCTTCATCTATGCTGCTCTGGGAGTGGAGCTCTTCGGAAAGCTGGTCTGCAATGACGAGAACCCG TGTGAGGGCATGAGCCGGCACGCCACCTTTGAAAACTTCGGCATGGCCTTCCTCACGCTCTTCCAGGTCT CCACAGGCGATAACTGGAATGGAATTATGAAGGACACCCTGCGAGACTGTACCCATGATGAGCGCAGCTG CCTAAGCAGCCTGCAGTTTGTGTCACCGCTCTACTTTGTGAGCTTCGTGCTCACAGCTCAGTTCGTGCTC ATCAACGTGGTGGTGGCCGTGCTGATGAAACATCTGGATGACAGCAACAAGGAGGCCCAGGAGGATGCAG AGATGGATGCTGAGATCGAGCTGGAGATGGCCCATGGCCTCGGCCCCTGCCCTGGCCCCTGCCCTGGTCC CTGCCCCTGCCCCTGGCCCCTAGCCCCTGTCCTGGCCCGAGGCTGCCCGCTAGTTCTCCTAGGGGCTACC GGGGCGAGGATCGGGAGGGGCAGGTGCTGGAGGCGACACCGAGAGTCACCTGTGCCGAGCACTCGCTATT CTCCAGCCCAGGAGACCCTGTGGCTGGACAGCGTCTCTTTAATCATCAAGGACTCCTTGGAGGGGGAGCT GACCATCATTGACAACCTGTCTGGGTCCGTCTTCCACCACTACGCCTCACCTGACGGCTGTGGCAAGTGT CACCATGACAAGCAAGAGGTGCAGCTGGCTGAGACAGAGGCCTTCTCCCTGAACTCAGACAGGTCTTCAT CCATCCTGCTGGGGGATGACCTGAGTCTTGAGGACCCCACGGCCTGCCCACCTGGCCGCAAAGACAGCAA GGGTGAGCTGGACCCACCTGAGCCCATGCGTGTGGGAGACCTGGGCGAATGCTTCTTCCCCTTGTCCTCT ACGGCCGTCTCGCCGGATCCAGAGAACTTCCTGTGTGAGATGGAGGAGATCCCATTCAACCCTGTCCAGT CCTGGCTCAAACATGACAGCAGTCAAGCACCCCCGAGCCCTTTCTCCCCGGATGGCTCCAGCCCTCTCCT GCAGATGCCTGCTGAGTTCTTCCACCCTGCTGTGTCTGCCAGCCAGAAAGGCCCAGAAAAGGGCACTGGC ACTGGAACCCTGCCCAAGATTGCACTTCAGGGGTCCTGGGCATCGCTGAGGTCACCGAGTGTCAACTGCA CCCTCCTCCGGCAGGCCACCGGGAGCGACACGTCGCTGGACGCCAGCCCCAGCAGCTCAGCGGGCAGCCT ACAGACCACACTGGAAGACAGTCTGACTCTGAGTGACAGTCCCCGGCGTGCCCTGGGGCCGCCGGTCCAG GTGCCTGGGCCACGGGCTAGCCTGTCACCGGCCACCCGGCGCCGCCTCAGCCTGCGGGGCCGTGGCCTGT TTAGTCTGCGTGGGCTGCGGGCCCATCAGCGTAGCCACAGCAGTGGCGGCTCCACCAGCCCTGGCTGCAC TCACCACGACTCCATGGACCCCTCTGATGAGGAGGGCCGCGGGGGCGCAGGTGGCGGGGGTGCAGGCAGT GAGCACTCGGAGACCCTCAGCAGCCTGTCACTCACATCTCTCTTCTGCCTACCACCCACGCTGCCACCAC CCGGCCTCACCCCAGCCAGGAAGTTCAACAGTACCAGCAGCCTGGCCGCTGGACCTGGCCGTCCTGGCTC CACTGTCTCAGCCCGTGGTCTGGTCAGAAGCCCCTCATGGGCTGCAGACCGCAGCAAGGACCCACCAGGC CAAGCTCAGCTGGTCTCAGGCTTGGGCTCCTCAGCACCCGGGCCACAGCCACCTCCCGGGGAGTCAACTG ATGCTGCGAGCAAAAGGAAGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203004 representing NM_020084 Red=Cloning site Green=Tags(s) MADSNLPPSSAAAPAPEPGITEQPGPRSPPPSPPGLEEPLEGTNPDVPHPDLAPVAFFCLRQTTSPRNWC IKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMECLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGK KCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNV LLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLTGDNGIMGCHE IPPLKEQGRECCLSKDDVYDFGAGRQDLNASGLCVNWNRYYNVCRTGNANPHKGAINFDNIGYAWIVIFQ VITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSS STVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALQNRRQAMGPGTPAPAKPGPHAKEPSHCKLCPR HSPLDPTPHTLVQPISAILASDPSSCPHCQHEAGRRPSGLGSTDSGQEGSGSGGSAEAEANGDGPQSSED GVSSDLGKEEEQEDGAARLCGDVWRETREKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQPEELTNIL EICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLV RFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIV TVFQILTQEDWNVVLYNGMASTTPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSS SNLEEFDKLPEGLDNSRDLKLCPIPMTPNGHLDPSLPLGAHLGPAGTMGTAPRLSLQPDPVLVALDSRKS SVMSLGRMSYDQRSLSSSRSSYYGPWGRSGTWASRRSSWNSLKHKPPSAEHESLLSGEGGGSCVRACEGA REEAPTRTAPLHAPHAHHAHHGPHLAHRHRHHRRTLSLDTRDSVDLGELVPVVGAHSRAAWRGAGQAPGH EDCNGRMPNIAKDVFTKMDDCRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENKFRI LCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFG EQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLIS SLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCVAANYRWVHHKYNFDNLGQALM SLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ EAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQ PTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINP TIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENP CEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVL INVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLGPCPGPCPGPCPCPWPLAPVLARGCPLVLLGAT GARIGRGRCWRRHRESPVPSTRYSPAQETLWLDSVSLIIKDSLEGELTIIDNLSGSVFHHYASPDGCGKC HHDKQEVQLAETEAFSLNSDRSSSILLGDDLSLEDPTACPPGRKDSKGELDPPEPMRVGDLGECFFPLSS TAVSPDPENFLCEMEEIPFNPVQSWLKHDSSQAPPSPFSPDGSSPLLQMPAEFFHPAVSASQKGPEKGTG TGTLPKIALQGSWASLRSPSVNCTLLRQATGSDTSLDASPSSSAGSLQTTLEDSLTLSDSPRRALGPPVQ VPGPRASLSPATRRRLSLRGRGLFSLRGLRAHQRSHSSGGSTSPGCTHHDSMDPSDEEGRGGAGGGGAGS EHSETLSSLSLTSLFCLPPTLPPPGLTPARKFNSTSSLAAGPGRPGSTVSARGLVRSPSWAADRSKDPPG QAQLVSGLGSSAPGPQPPPGESTDAASKRKR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_020084
ORF Size	6603 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_020084.3, NP_064469.3
RefSeq Size	6709 bp
RefSeq ORF	6606 bp
Locus ID	56827
UniProt ID	Q9Z0Y8
MW	243.7 kDa
Gene Summary	low voltage-activated calcium channel subunit; important for calcium ion transport into nerve, muscle and endocrine cells [RGD, Feb 2006]

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