Ercc6 (NM_001107296) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203047 representing NM_001107296 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCATGAGGAAATCCCCAACTCCACTCAACCTCAGGGCCAAGACTGGTTACCAGGTCAGCATGTCA GTGCTGACAGAGACATACCAGCTGGGCAGGAGACTGCTGGGGACTCAGAGGCGGGGAAGTGCCTCTCCTC TCCTTCCTGTGACCATGGGCCGTCCACATCTGCTGAAGCTTGTGTACTGGCAGCCACAAGGTGGGGGCCC GCCCTGCTGCACATCGACCGGCATCAGATTCCTGCGGTGGAGCCCAGTGCTCAGGCCCTGGAGCTGCAGG GCCTGGGTGTGGACGTCTACGACCAGGCCGTCCTGGAGGAAGGTGTGCTTCAGCAGGTGGACAGTGCAAT GCATGAGGCCAGCCGTGTGGCCCAGCGTGCTGATGCGGAGAAAGAGTATCAGTCAGTCCTGGACGACCTC ATGTCATGTACAACATCCCTGAGGCAAATCAATAAAATTATAGAGCAACTTAGCCCTCAAGCTGCCAGCA ACAGAGACATCAACAGGAAACTAGATTCTGTGAAACGACAGAAGTACAACAAGGAACAACAGCTAAAGAA GATAACTGCAAAACAGAAACGTCTCCAGGCCATTCTCGGAGGAGCGGAGGTCCATGTGGAACTGGATCAT GGCAGCTTAGAGGAGGAAGATGCAGAGCCAGGCCCATCATGTCTCGGCAGTATGCTCATGCCTGCCCAGG AGAGCGCCTGGGAAGAACTTATCCGCACTGGCCAGATGACACCGTTCGGTACCCCAGCCCCTCAGAAACA GGAGAAAAAGCCTAGAAAAATAATGCTCAATGAAGCATCAGGCTTTGAAAAATATTTGGCAGACCAAGCA CAACTTTCTTTTAAAAGGAAAAAACAAGCTGCTAATAAAAGAACAGCTAAAAAAGCTGTGGTTGTATCTG AGTCAAGCCGAGGACCAAATGAAACTAAGCCAAACCAGAGAAGCCAAGCGCTCTCTCAGACAGACAAGCG CCTGAAGAAGCACATCAGGAAGCTCCAGAGGAGGGCGCTGCAGTTCCAGGGCAAAGTGGGATTGCAAAGG GGGAAGAGACCTCTGGAGCCTGAGGAGAGGCCAGGGGCAGAGGGAGACACTGAGGCCGTTCTGTCTTCCG ATGACATTAGCTATGAACTGAAGCCTCTTCGGAAAGGACAAAAATACGAGAAGAAAGTCCCAGTGCAGGA GATCGACGATGACTTCTTTCCAAGTTCTGAGGAGGAAGATGAAGCTGTGGAAGGAAGAGGAGGAGGCCGC AAGGTCTCCAGGCGCCCAGACGATGGAGATGAGGATTATTATAAACAACGATTAAGGAGGTGGAATAGGC TGAGGCTGCAGGACAAAGAGAAGCTGAAGCTTGAAGACGATTCTGAGGAGAGCGATGCTGAGTTTGACGA AGGTTTCAAAGTGCCGGGTTTTCTGTTCAAAAAGCTCTTTAAGTTCGAGGGACTCGGCCCCACTCTCATT GTCTGCCCAACGACAGTGATGCATCAGTGGGTGAAAGAATTCCACACGTGGTGGCCTCCATTCAGAGTGG CGATTCTGCACGAAACAGGCTCCTGTGCCCACAGAAAGGAGAGGCTGGTTCGAGATATTGTTCATTGTCA TGGAGTTTTGATAACTTCGTATTCCTACATTCGACTGATGCAAGACGACATTAGCAGGCATGACTGGCAC TACGTCATCTTAGACGAAGGACATAAAATTCGAAATCCAAATGCTGCAGTCACCCTTGCTTGCAAACAGA ATAACCTGCGAGAGCTGTGGTCACTCTTCGACTTTACCTTCCCAGGAAAGCTAGGCACGTTGCCTGTGTT CATGGAGCAGTTCTCTGTCCCCATCACCATGGGGGGGTACTCCAATGCTTCCCCAGTACAGGTTAAAACT GCCTATAAGTGTGCGTGTGTCTTGAGAGACACGATAAACCCGTACCTACTGCGAAGAATGAAATCAGATG TCAAGATGAGCCTTTCGCTGCCAGATAAAAATGAACAGGTCTTATTTTGCCGTCTAACTGATGAGCAGCA TAAAGTCTACCAGAATTTCATCGATTCCAAAGCAGTTTACAGGATTCTCAATGGAGAGAATCAGATTTTC TCTGGACTTGTAGCTCTGAGGAAGATCTGCAACCACCCTGACCTCTTTTCTGGCGGTCCCAAGAACACCA GTGCTCTTCCAGAGGATGGACTGGAGGAGGAGCAGTTCGGATACTGGAAACGCTCTGGGAAAATGATTGT TGTTGAGTCTTTGCTGAAAATCTGGCACAAGCAGGGTCAGCGGGTGCTGCTGTTTTCCCAGTCAAGGCAG ATGCTGCACATACTGGAAGTGTTCCTGAGAGCCCACAAGTATTCCTATCTCAAGATGGATGGGACCACTA CCATAGCATCCAGGCAGCCGCTGATTACAAAATACAATGAGGACACGTCCATCTTTGTCTTTCTTCTGAC CACACGGGTGGGCGGCTTAGGAGTGAACCTGACCGGGGCCAACAGAGTCATCATCTACGACCCTGACTGG AATCCAAGCACCGACACACAGGCACGGGAGCGAGCATGGAGGATCGGCCAGAAGAAGCAGGTGACTGTCT ACAGGCTTCTGACGGCCGGCACCATAGAGGAAAAGATCTATCACCGACAAATCTTCAAGCAATTTTTGAC AAACAGAGTGCTAAAAGACCCTAAACAAAGGCGCTTTTTCAAGTCCAATGATCTCTATGAGCTGTTTACT CTGACTAGTCCTGATGCCTCCCAGGGCACTGAAACAAGTGCTATTTTTGCAGGAACCGGATCTAATATTC AGACACTGAAATGCCATTTTAAAAAGAGGACTCCGCCAGTCCTACAAATGGATCCCAAACGCAAGAAGCC CCTGGTTTCTGACACATCTGTAAATGGTGCCACTGTGATGGAAGAAAAATCTCAGGGGTCAGGAGCTACG GGCAGTGTCCCTTCTACAGAAAGTGGCCCTTTGAAAAGTGACCGTCACACAGATGGGAGCAGAGCCAGCA GTGTTGGTTTTGGAGAAGAGGCAGATGCAGGGTCCACGCTGGAGCATCTCTCAGGGCTGAGTGGAGATTC TCCAGCAGTTGACCACACATCCAGGCTGTCAGCTGTGGCGAGCACCAGTGAGAAGCAGGGCTCTTCCTAT GAAGGGGAGTGCTGCCAGGCTCAGACGGGACCTGTTCACCTGAGTGAACAAACGGAAGGTCATTTCTCTA AGCACAAATCAAAAAGGAAACATGATGCAGCGGAGGAAGAGACCACAGAGAAGTGTCCACAGCCCAAGCA AAAGGCCAAGAACTCCAGGCATCACAGAGATGCTAAGTTTGAGGGAACTCGAGTCCCACACCTGGTGAAG AAGAGGCGGTACCATCAGCAAACCACTGAGCAGGAGAGCGGAGCCAAGGACCGGAGCAGTGATGATTATG TTTTGGAAAAGCTTTTCAAAAAATCAGTGGGCGTGCACAGCGTCGTGAAGCATGATGCCATCATAGATGG GTCCAGTCCGGATTATGTGCTGGTGGAGGCAGAAGCCAACAGAGTGGCTCAGGATGCCTTGAAAGCTCTG AGGCTCTCTCGTCAGCAGTGCCTGGGGGCAACATCTGGTGTTCCCACCTGGACTGGCCACAGGGGTATTT CCGGTGCACCAGCAGGAGTAAAAATGCGAGCACGAAACCACATGATTTTGCCGGAGCGTTTGGAGAGTGA CGGCGAGCAGCTTGCTGGGGCTGCGGCTGCGGCTGCGCCTCCCTGCAGCACAGAGCACGACGCCCTTCTG GTGGACATGAGGAACTTCATTGCTTTCCAGGCTCAGGTGGATGGCCAGGCCAGCACTCAGGAAATCCTAC AGGCGTTCGAATCCAAGCTGTCTGTGGCACAGTCCTGTGTCTTCCGAGAACTACTGAGGAATCTGTGCAA TTTCCATAGAACTCCTGGTGGTGAAGGGATTTGGAAACTGAAGCCAGAGTACTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203047 representing NM_001107296 Red=Cloning site Green=Tags(s) MPHEEIPNSTQPQGQDWLPGQHVSADRDIPAGQETAGDSEAGKCLSSPSCDHGPSTSAEACVLAATRWGP ALLHIDRHQIPAVEPSAQALELQGLGVDVYDQAVLEEGVLQQVDSAMHEASRVAQRADAEKEYQSVLDDL MSCTTSLRQINKIIEQLSPQAASNRDINRKLDSVKRQKYNKEQQLKKITAKQKRLQAILGGAEVHVELDH GSLEEEDAEPGPSCLGSMLMPAQESAWEELIRTGQMTPFGTPAPQKQEKKPRKIMLNEASGFEKYLADQA QLSFKRKKQAANKRTAKKAVVVSESSRGPNETKPNQRSQALSQTDKRLKKHIRKLQRRALQFQGKVGLQR GKRPLEPEERPGAEGDTEAVLSSDDISYELKPLRKGQKYEKKVPVQEIDDDFFPSSEEEDEAVEGRGGGR KVSRRPDDGDEDYYKQRLRRWNRLRLQDKEKLKLEDDSEESDAEFDEGFKVPGFLFKKLFKFEGLGPTLI VCPTTVMHQWVKEFHTWWPPFRVAILHETGSCAHRKERLVRDIVHCHGVLITSYSYIRLMQDDISRHDWH YVILDEGHKIRNPNAAVTLACKQNNLRELWSLFDFTFPGKLGTLPVFMEQFSVPITMGGYSNASPVQVKT AYKCACVLRDTINPYLLRRMKSDVKMSLSLPDKNEQVLFCRLTDEQHKVYQNFIDSKAVYRILNGENQIF SGLVALRKICNHPDLFSGGPKNTSALPEDGLEEEQFGYWKRSGKMIVVESLLKIWHKQGQRVLLFSQSRQ MLHILEVFLRAHKYSYLKMDGTTTIASRQPLITKYNEDTSIFVFLLTTRVGGLGVNLTGANRVIIYDPDW NPSTDTQARERAWRIGQKKQVTVYRLLTAGTIEEKIYHRQIFKQFLTNRVLKDPKQRRFFKSNDLYELFT LTSPDASQGTETSAIFAGTGSNIQTLKCHFKKRTPPVLQMDPKRKKPLVSDTSVNGATVMEEKSQGSGAT GSVPSTESGPLKSDRHTDGSRASSVGFGEEADAGSTLEHLSGLSGDSPAVDHTSRLSAVASTSEKQGSSY EGECCQAQTGPVHLSEQTEGHFSKHKSKRKHDAAEEETTEKCPQPKQKAKNSRHHRDAKFEGTRVPHLVK KRRYHQQTTEQESGAKDRSSDDYVLEKLFKKSVGVHSVVKHDAIIDGSSPDYVLVEAEANRVAQDALKAL RLSRQQCLGATSGVPTWTGHRGISGAPAGVKMRARNHMILPERLESDGEQLAGAAAAAAPPCSTEHDALL VDMRNFIAFQAQVDGQASTQEILQAFESKLSVAQSCVFRELLRNLCNFHRTPGGEGIWKLKPEYC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107296
ORF Size	3975 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107296.1, NP_001100766.1
RefSeq Size	7003 bp
RefSeq ORF	3978 bp
Locus ID	306274
MW	148.5 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.