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Lpcat1 (NM_001100735) Rat Tagged ORF Clone
CAT＃: RR203122
- TrueORF®
Lpcat1 (Myc-DDK-tagged ORF) - Rat lysophosphatidylcholine acyltransferase 1 (Lpcat1), (10 ug)
"NM_001100735" in other vectors (3)
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CNY 6,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Aytl2; RGD1311599 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR203122 representing NM_001100735
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTGCGGGGTCGCGGGCCGCGGGCCGCCCCTTCCTCCAGCTCTGGGGCCGGCGACGCGCGACGGC TAGCGCCCCCGGGGCGGAACCCCTTCGTGCACGAGCTGCGACTGAGCGCCCTGCAGAAGGCCCAGGTGGC TTTCATGACGCTGACGCTGTTCCCCATCCGGCTCCTGTTTGCTGCTTTCATGATGCTGCTGGCCTGGCCT TTTGCACTTGTGGCCTCCCTGGGACCTCCTGATAAGGAGCCAGAGCAGCCCCTGGCCTTATGGAGGAAGG TCGTGGACTTCCTGCTCAAGGCCATCATGCGCACCATGTGGTTTGCTGGCGGCTTCCACCGAGTAGCTGT GAAGGGGCGGCAGGCATTGCCTACTGAGGCTGCTATCCTCACCTTGGCCCCACATTCCTCCTACTTTGAT GCCATCCCGGTCACCATGACCATGTCCTCCATTGTGATGAAGGCGGAAAGCAGAGACATCCCAATCTGGG GAACTCTGATAAGATACATCAGGCCCGTGTTCGTGTCCCGCTCTGACCAGGACTCTCGAAGAAAGACAGT GGAAGAGATCAAGCGACGGGCGCAGTCCAATGGAAAGTGGCCTCAGATAATGATTTTTCCAGAGGGAACG TGTACAAATAGGACCTGCCTCATTACCTTCAAACCTGGTGCATTCATTCCCGGAGTTCCTGTCCAACCCG TGGTGCTACGCTACCCGAACAAATTGGACACCATCACATGGACGTGGCAAGGACCCGGAGCGTTGAAAAT CCTGTGGCTCACCCTGTGCCAGTTTCAAAACCAAGTGGAAATTGAATTTCTGCCTGTGTATTGTCCTTCT GAGGAGGAGAAGAGGAATCCTGCCCTGTATGCCAGTAATGTGAGACGCGTCATGGCCAAGGCCCTGGGTG TCTCAGTAACAGACTACACGTTTGAGGACTGCCAGCTGGCTCTGGCAGAAGGACAACTTCGCCTGCCTGC CGATACCTGCCTGCTAGAGTTTGCCAGGCTCGTGAGGGGCCTCGGACTAAAACCAGAAAATCTTGAAAAA GATCTAGACAAATATTCAGAGAGCGCAAGGATGAAGAGAGGAGAGAAGATCAGGCTTCCAGAGTTTGCAG CCTACTTGGAAGTTCCCGTCTCAGACGCGCTAGAGGACATGTTCTCGCTTTTTGATGAGAGTGGTGGCGG TGAGATAGACCTTCGAGAATATGTGGTCGCCTTGTCTGTGGTGTGCAGGCCATCCCAGACCCTAGCCACC ATCCAGCTGGCATTTAAGATGTATGGGTCTCCTGAGGATGGCAGCATAGATGAAGCCGACCTGTCCTGCA TCCTCAAGACTGCACTGGGCATATCAGAGTTGACAGTGACCGACTTGTTCCAGGCTATTGACCAAGAGGA GAGGGGAAGAATCACATTTGATGACTTCTGTGGGTTTGCAGAAATGTACCCTGACTTCGCAGAGGACTAC TTGTACCCTGATCAGACACATTCTGACAGCTGCGCACAGACACCCCCAGCACCAACTCCCAATGGCTTCT GCATTGACTTCAGCCCCGAACACTCAGACTTTGGGAGAAAGAATTCGTGTAAGAAAGTGGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR203122 representing NM_001100735
Red=Cloning site Green=Tags(s) MRLRGRGPRAAPSSSSGAGDARRLAPPGRNPFVHELRLSALQKAQVAFMTLTLFPIRLLFAAFMMLLAWP FALVASLGPPDKEPEQPLALWRKVVDFLLKAIMRTMWFAGGFHRVAVKGRQALPTEAAILTLAPHSSYFD AIPVTMTMSSIVMKAESRDIPIWGTLIRYIRPVFVSRSDQDSRRKTVEEIKRRAQSNGKWPQIMIFPEGT CTNRTCLITFKPGAFIPGVPVQPVVLRYPNKLDTITWTWQGPGALKILWLTLCQFQNQVEIEFLPVYCPS EEEKRNPALYASNVRRVMAKALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGLKPENLEK DLDKYSESARMKRGEKIRLPEFAAYLEVPVSDALEDMFSLFDESGGGEIDLREYVVALSVVCRPSQTLAT IQLAFKMYGSPEDGSIDEADLSCILKTALGISELTVTDLFQAIDQEERGRITFDDFCGFAEMYPDFAEDY LYPDQTHSDSCAQTPPAPTPNGFCIDFSPEHSDFGRKNSCKKVD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001100735 |
| ORF Size | 1602 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001100735.1, NP_001094205.1 |
| RefSeq Size | 3621 bp |
| RefSeq ORF | 1605 bp |
| Locus ID | 361467 |
| UniProt ID | Q1HAQ0 |
| MW | 59.8 kDa |
| Gene Summary | Possesses both acyltransferase and acetyltransferase activities (PubMed:16864775). Activity is calcium-independent (By similarity). Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC) (PubMed:16864775). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively (PubMed:16864775). May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology (PubMed:16864775). Involved in the regulation of lipid droplet number and size (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN203122 | Lpcat1 (untagged ORF) - Rat lysophosphatidylcholine acyltransferase 1 (Lpcat1), (10 ug) |
CNY 6,840.00 |
|
| RR203122L3 | Lenti ORF clone of Lpcat1 (Myc-DDK-tagged ORF) - Rat lysophosphatidylcholine acyltransferase 1 (Lpcat1), (10 ug) |
CNY 8,740.00 |
|
| RR203122L4 | Lenti ORF clone of Lpcat1 (mGFP-tagged ORF) - Rat lysophosphatidylcholine acyltransferase 1 (Lpcat1), (10 ug) |
CNY 9,600.00 |
