Mamdc4 (NM_145768) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Aegp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203710 representing NM_145768 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCCTGCCTAGCTGCCTCCTCTCAATCTGGGTCCTATTTATGGCTGCACAGTCTCTAGGCAAGACCT GGGTCCCTGACCACTGCAGAAGCCCCACCGAAGCTACGTGCAACTTTGTGTGTGACTGTGGAGACTGTTC AGATGAGGCTCAGTGTGGTTTCCACGGTGCCTCAACTACCCCAAACACCCCCTTCACCTGCAACTTTGAG CAGGATCCCTGTGGCTGGCAGGACATCAGCACCTCGGGCTATCGATGGCTTCGAGACCGGGCAGGGGCAG GGCTGGATAGTTCTGGGCCCCATTCTGACCATACACGTGGCACTGACTTGGGCTGGTACATGGCTGTGGG AACCCATAGTGGGAAGGAGCCATCTACTCGGACTTTGCGCTCTCCTGTCATGCGTGAGGCAGCCCCCACC TGTGAACTGAGGCTCTGGTACCACACTGACTCTAGAGATGTCGCTGAGCTGCGGCTGGATCTGACCCATG GTATGGAGACACTGACTCTATGGCAGAGCTCGGGACCTTGGGGTCCCTGGCCTGGCAGGGAACTAGCAGT GAACACTGGCCGAATCCAGGGTGACTTCAAAGTGACATTTTCTGCCACTCGAAATGCTACACACAGGGGC GCTGTGGCCTTGGATGACATGGAGTTCTGGGACTGTGGTCTGCCCATCCCTCAGGCCAGGTGTCCCCTGG GGCACCACCACTGCCAGAACAAAGCTTGTGTGGAGCCCCACCAGCTCTGTGACGGTGAAGACAACTGTGG AGACAGTTCTGATGAAGACCCGCTCATCTGCAGCCACCACATGGCCACCGACTTTGAGACAGGCCTAGGA CCATGGACCCAATTAGAGGGCTGGACACGGAACTTCAGTGCTGGCAGCATGGTGAGCCCTGCCTGGCCCC ACCGAGATCACAGCCGGAATAGTGCATATGGCTTCTTTCTCGTCTCTGTGGCCAAGCCTGGCACCACTGC TGTCCTCTACAGCCCTGAGTTCCAAGGCTCAGTCTCCTACAACTGCTCGTTCACCTTCTATTACTACCTG CATGGGTCTGAGGCCAACCAATTCCAGCTCTTTGTGCAGGCACAGGGGCTCAACACCACCCAGCCTCCTG TCCTACTCCGAAGCCGCCATGGAGAGTTGGGGACAGCCTGGGTCAGAGACCGGGTTAACATTCAAAGTGC CCATCCATTTCGGATCCTTCTTGCTGGGGAGACTGGTCCAGGAGGCTTCGTGGGCCTAGACGACCTTATC ATGTCCAACCACTGCATACTTGTTCCAGGTATGTCCACCCTGCAGTCATCACTTTCTGGACCTGTGCCAT TGGCCCTCTATCCCCAGACATCCATCAAGCGAACCTGTGACGCAGGACATCTCTCCTGTGACGAGCTGTG TGTGCCCCCGGAGCAGCTGTGTGATTTCCAGCAGCACTGTGCCGAAGGCGAGGATGAAGAGAAGTGTGGC ACCACGGACTTTGAGTCTGCCTCTGCTGGGGGCTGGGAGGACATCAGTATAGGAAAGCTACAGTGGCAAC GGGCTGAAGCCCAGGAGAGCGGGAAGCCTGCCAGGGATACCAACCGGAATGCACCTGGGCACTTCCTATC TCTGCGGAAGGCCTGGGGGCAGCTACGATCTGAGGCCCGGGCTCTCACACCTACTCTGGGCCCCTCTGGC CCTCACTGTGAACTCCACATGACTTACTATTTTCACAGTCATCCCCAAGGCTTCTTGGCACTGGCTGTGG TGGAGAATGGCTTCCGGGAGCTGCTGTGGCAGGCTCCAAGCAGCAGCAGTGGGGGCTGGACACTACAGAA GATTCTTCTTGGGGCACGGCGCTGGCCATTCCAGCTGGAGTTCGTCAGTCTGGTGGACTTGGATGGCCCT GGCCAACAGGGGGCTGGGGTGGATAATGTGACCCTGAGAGACTGCAACCCCATGGTGACCACTGAGAGTG ACCAAGAGGTCTCCTGTAACTTTGAACGGGACTCCTGCAGCTGGCACACAGGCCACCTCACAGATGCCCA CTGGCACCGTGTAAAAAGCCACGGCTCTCAGTATGACCACACCACCGGCCAAGGTTTTTTCATGTTCCTG GATCCCATGGACCCACCTGCTCGAGGCCAAGGTGCCCTGTTGCTCACAAGACCCCAGGTGCCAGTTGTCC CCAAGGAATGTCTCAGCTTCTGGTACCATCTGCATGGGCCCCAGATTGGGACACTGTGCTTGGCCATGAG GAGGGAGGGGGAGGAAGACACACTACTGTGGTCCCGGTCAGGCACCCACGGCAACCGCTGGCACCAGGCT TGGGTCACTCTTCACCACCAGCTGCAGCCCAGCACCAAGTACCAGTTGCTGTTTGAAGGCCTCCGGGATG GGTACCATGGCACAATGGGCCTGGATGACATGGCTGTGCGGCCTGGCCCCTGCTGGGCCGCCAAGAGATG TTCCTTTGAAGACTCTGACTGTGGCTTCTCCCCAGGGGACTGGGGGCTGTGGACACGCCAGAATAATGCC TCAGGCCTTGGTCCTTGGGGTCCTTGGATAGACCATACCACAGGGACAGCCCAGGGGCACTACATGGTGG TGGACACCAGCCCAAATTTACTGCCCAAAGGTCATGTGGCCTCTCTGACTTCAGAGGAGCACCCGCCCCT GTCCCGGCCTGCCTGCCTGTCCTTCTGGTACCACCTGAGCTTCCACAATCCAGGCACCTTGAGGGTCTTC GTGGAAGAGAGCACAAGGCGCCAAGAGCTCAGCATCAGTGGCCATGGTGGATTTGCGTGGCGCCTAGGCA GCGTGAATGTACAGGCTGAGCAGGCCTGGAAGGTAGTGTTTGAGGCTATGGCTTCTGGCGTGGAACATTC CTACATGGCTCTGGATGACATTTCCCTCCAAGATGGGCCCTGTGCTCAGCCAGGGTCCTGTGACTTTGAA TCTGGCCTGTGTGGGTGGAGCCACCTGCCTTGGCCTGGTCTGGGTGGGTACAGCTGGGACTGGAGCAGTG GAGCCACTCCATCCCGGTACCCCCGGCCTTCAGTGGACCACACTGTGGGCACAGAAGCAGGCCACTTCGC TTTCTTTGAAACCAGCGTGCTAGGTCCTGGGGGCCAGGCAGCCTGGCTCGGCAGTGAGCCACTGCCAGCA ACTGCAGTCTCCTGCCTCCACTTCTGGTACTACATGGGCTTTCCTGCGCATTTCTACAAGGGTGAGTTGA GAGTGCTCCTGAGCAGTACCCAAGGACAGCTAGCTGTGTGGCACCGGGGTGGGCACTTGCGGGACCAGTG GCTACAAGTCCAGATTGAAGTGTCCAGCTCTGAGGAGTTCCAGATTGTTTTTGAAGCCACTCTGGGTGGC CAGCCGGCTCTAGGGCCCATTGCCCTTGATGATGTGGAGTACTTGGCTGGACAACACTGCAAGCAACCTA CACCTAGCCAGGGTAGAGTGGCAGCACCCGTGTCTGTGCCGGTTGCAGTCGGAGGAGCCCTCCTCCTCTT CCTGTTGCTCCTGGGCCTTGGAGGTTGGCACTGGCTGCAGAAGCAGCACCTCCCCTGCCAAAGTACAGAT GCAGCAGCCTCTGGCTTTGACAATATCCTCTTCAATGCGGATCAAGTTACCCTCCCAGAATCAATCACCA GTAACCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203710 representing NM_145768 Red=Cloning site Green=Tags(s) MCLPSCLLSIWVLFMAAQSLGKTWVPDHCRSPTEATCNFVCDCGDCSDEAQCGFHGASTTPNTPFTCNFE QDPCGWQDISTSGYRWLRDRAGAGLDSSGPHSDHTRGTDLGWYMAVGTHSGKEPSTRTLRSPVMREAAPT CELRLWYHTDSRDVAELRLDLTHGMETLTLWQSSGPWGPWPGRELAVNTGRIQGDFKVTFSATRNATHRG AVALDDMEFWDCGLPIPQARCPLGHHHCQNKACVEPHQLCDGEDNCGDSSDEDPLICSHHMATDFETGLG PWTQLEGWTRNFSAGSMVSPAWPHRDHSRNSAYGFFLVSVAKPGTTAVLYSPEFQGSVSYNCSFTFYYYL HGSEANQFQLFVQAQGLNTTQPPVLLRSRHGELGTAWVRDRVNIQSAHPFRILLAGETGPGGFVGLDDLI MSNHCILVPGMSTLQSSLSGPVPLALYPQTSIKRTCDAGHLSCDELCVPPEQLCDFQQHCAEGEDEEKCG TTDFESASAGGWEDISIGKLQWQRAEAQESGKPARDTNRNAPGHFLSLRKAWGQLRSEARALTPTLGPSG PHCELHMTYYFHSHPQGFLALAVVENGFRELLWQAPSSSSGGWTLQKILLGARRWPFQLEFVSLVDLDGP GQQGAGVDNVTLRDCNPMVTTESDQEVSCNFERDSCSWHTGHLTDAHWHRVKSHGSQYDHTTGQGFFMFL DPMDPPARGQGALLLTRPQVPVVPKECLSFWYHLHGPQIGTLCLAMRREGEEDTLLWSRSGTHGNRWHQA WVTLHHQLQPSTKYQLLFEGLRDGYHGTMGLDDMAVRPGPCWAAKRCSFEDSDCGFSPGDWGLWTRQNNA SGLGPWGPWIDHTTGTAQGHYMVVDTSPNLLPKGHVASLTSEEHPPLSRPACLSFWYHLSFHNPGTLRVF VEESTRRQELSISGHGGFAWRLGSVNVQAEQAWKVVFEAMASGVEHSYMALDDISLQDGPCAQPGSCDFE SGLCGWSHLPWPGLGGYSWDWSSGATPSRYPRPSVDHTVGTEAGHFAFFETSVLGPGGQAAWLGSEPLPA TAVSCLHFWYYMGFPAHFYKGELRVLLSSTQGQLAVWHRGGHLRDQWLQVQIEVSSSEEFQIVFEATLGG QPALGPIALDDVEYLAGQHCKQPTPSQGRVAAPVSVPVAVGGALLLFLLLLGLGGWHWLQKQHLPCQSTD AAASGFDNILFNADQVTLPESITSNP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_145768
ORF Size	3648 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_145768.1, NP_665711.1
RefSeq Size	3906 bp
RefSeq ORF	3651 bp
Locus ID	252882
UniProt ID	Q63191
MW	133.8 kDa
Gene Summary	glycoprotein localized to the apical endosomal tubules of intestinal epithelial cells; may play a role in intestinal transport [RGD, Feb 2006]

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