Eprs (NM_001024238) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205417 representing NM_001024238 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCTGTGCCTTACGGTGAACGCCGGAAACCCTCCACTGGGTTGTCGAGTTAAAGTGAACAAGA GAAATAAATCCTTCATGTTGTCATTTTTAGAAGCTCTGCTGGCAGTGGAGCATGTGAAAGGTGATGTCAG CATTTCTGTGGAAGAAGGGAAGGAGAATCTTCTTCGGGTTTCTGAGAGTGTGGTGTTCACTGACACAAAT TCAATCCTGCGCTACTTGGCTAGAGTTGCAGCTACATCTGGGCTGTACGGTTCTAACCTGATGGAGCACA CGGAGATTGATCATTGGTTGGAGTTCAGCGCTACAAAGTTGTCTTCGTGTGATGTGCTGACTTCGGCAAT CAAGGAGCTTAACCATTGCCTGTCTCTGAGAACATACCTGGTTGGGAACTCTTTGACTTTAGCAGATTTA TGTGTTTGGGCCACCCTAAAAGGAAATGCTGCATGTCAGGAGCACTTGAGACAGAGCAAAACCCTAGTCC ACGTTAACCGCTGGTATGGCTTTCTTGAAGCCCAGCAGGCCTTCCGTTCAGTTGGTACCAAGTGGGATGC TTCAGCAAACAAAGCTACAGGGGCACCTGACAGAAAACAAGATATTGGGAAATTCGTCGAGCTTCCGGGT GCAGAGATGGGAAAGGTGACAGTCAGATTCCCCCCAGAGGCTAGTGGTTACTTACACATTGGGCATGCAA AGGCTGCTCTTCTGAACCAGCACTACCAGGTTAACTTTAAAGGCAAACTGATCATGAGATTTGATGACAC AAACCCAGAAAAGGAAAAGGAAGATTTTGAGAAGGTCATCTTGGAAGATGTTGCAATGCTGCACATTAAA CCAGATCAGTTTACTTACACTTCAGATCATTTTGAAACTATAATGAAGTATGCAGAGAAATTAATTGAAG AAGGGAAGGCCTATGTGGACGACACTCCCGCAGACGAGATGAAGGCGGAGCCTGTTGAGAAGAATCTACA AATGTGGGAAGAAATGAAAAGAGGAAGCCAGTTTGGTCAGTCCTGTTGCCTGCGAGCAAAAATTGACATG AGTAGTAACAATGGGTGTATGAGGGACCCAACCCTTTACCGCTGCAAAATCCAGCCCCACCCAAGAACTG GGAATAAATACAATGTTTACCCAACGTATGACTTTGCCTGTCCTATAGTGGACAGCATTGAAGGTGTTAC TCATGCCTTGAGGACGACAGAGTACCACGACCGAGATGAGCAGTTTTACTGGATTATTGAAGCCTTAGGC ATAAGGAAACCATACATCTGGGAATACAGTCGCTTAAATCTCAACAACACAGTGCTGTCCAAGAGAAAGC TTACGTGGTTTGTCAATGAAGGACTGGGCTCCTCAAGGTCTGTTGTAAACATGGAATGGGACAAAATCTG GGCGTTTAACAAAAAGGTTATTGACCCTGTGGCTCCAAGGTACGTTGCATTGCTGAAGAAGGAGGTGATC CCAGTGAACATCCTGGACGCGCAGGAGGAGATGAAGGAAGTAGCCAGACATCCCAAGAATCCTGATGTTG GTTTGAAGCCTGTGTGGTATAGTCCCAAGGTTTTCATAGAAGGAGCTGATGCAGAGACTTTCTCAGAGGG TGAAATGGTCACATTTATAAACTGGGGCAACATCAACATTACTAAAATACACAAAAATCCAGATGGAAAA ATTACATCTCTGGATGCAAAATTGAATTTGGAAAACAAAGACTACAAGAAAACAACTAAGATCACTTGGC TTGCGGAGACCACACAGGCTCTCCCTGTCCCGGCAGTCTGTGTCACTTATGAGCACTTGATTACAAAGCC AGTGCTAGGAAAAGACGAGGACTTCAAGCAGTACGTCAACAAAGACAGTAAGCATGAAGAACTAATGCTA GGAGATCCCTGCCTTAAGGATTTGAAGAAAGGAGACATCATACAATTGCAGAGGCGCGGATTCTTCATAT GCGACCAGCCGTACGAACCTGTCAGCCCATATAGTTGCAAAGAAGCCCCCTGCATCTTGATATATATACC CGATGGGCACACCAAGGAGATGCCAACATCTGGATCAAAGGAAAAGACTAAAGTGGAAATCTCAAAAAAG GAGACCGGCTCTGCTCCTAAGGAGAGACCCGCAGCTGCTGTGAGTGCTCCCTGTGCTGCAGCTGAGGACG CTGCCATCCTCTACAGCAGAGTGGCGGTCCAGGGTGATGCGGTTCGCGACCTGAAAGCGAAGAAAGCACC AAAGGAGGATATTGACGCAGCTGTGAAACAGCTTTTGACTTTGAAGGCTGAGTATAAAGAGAAAACTGGG CAGGAGTATAGACCTGGGAACCCCCCTACTGCAGCAGTGCAGACTGTTTCTACAAAGTCCCCATCCAACA CCGGGGAATACACATCTTTATACAATAAAGTTGCTGCCCAAGGGGAGCTGGTCCGGAAGCTGAAAGCTGA AAAGGCTCCTAAGGCTAAAATAAATGATGCTGTAGAATGCTTACTGTCCCTGAAAGCTGAGTATAAAGAA AAAACTGGGAAGGAGTATGTACCTGGCCAACTCCCAGCATCTCAGAACTCACATTCAAGTCCTGTCAGCA ATGCCAAGCCTGTTGGATCAGAGACACCGGAAGCCAAACTGCTGTTTGACAGAGTGGCTTGTCAAGGAGA AGTAGTTCGGAAACTTAAAGCTGAGAAGGCCTCTAAGGATCAAGTGGATTCAGCCGTACAAGAACTTCTT CAGCTGAAGGCACAGTACAAGTCACTGACAGGAATTGAGTATAAGCCTGTGTCTGCTACTGGGGCAGAAG ACAAAGATAAGAAGAAGAAAGAAAAAGAAAATAAGTCTGAAAAGCAGAATAAGCCTCAGAAACAAAATGA TGGCCAAGGGAAAGACTCTTCTAAAAGCCAAGGCAGCGGGCTGTCGTCCGGTGGAGCAGGAGAAGGGCAA GGGCCCAAGAAACAGACCAGATTGGGTCTTGAAGCAAAAAAAGAAGAAAATCTTGCCGAATGGTATTCCC AAGTCATCACGAAGTCAGAGATGATCGAATACTACGATGTGAGTGGCTGCTATATTCTCCGACCCTGGTC GTACTCCATCTGGGAATCCATTAAGGACTTCTTTGACGCGGAGATCAAGAAGCTCGGCGTTGAGAACTGC TACTTCCCCATATTCGTGTCCCAGGGCGCACTAGAGAAGGAGAAGAACCACATTGCTGACTTTGCTCCCG AGGTTGCTTGGGTGACAAGATCTGGAAAGACAGAGCTGGCAGAGCCGATTGCCATCCGTCCTACTAGTGA AACAGTAATGTATCCTGCATATGCAAAATGGGTGCAGTCCCACAGAGACCTGCCCATCAGGCTCAACCAG TGGTGCAACGTGGTGCGCTGGGAGTTCAAACACCCACAACCTTTCCTCCGCACTCGGGAGTTCCTATGGC AGGAGGGGCACAGCGCCTTTGCCACCTTTGAGGAGGCAGCTGACGAGGTGTTACAGATCCTCGAGTTATA TGCTCGAGTGTATGAGGAGCTCTTGGCAATCCCCGTCGTGAGAGGAAGGAAGACTGAGAAGGAAAAGTTT GCAGGAGGAGACTATACAACCACACTAGAAGCCTTCATATCTGCCAGCGGACGCGCTATCCAGGGAGCAA CATCACACCATTTAGGCCAGAATTTCTCCAAAATGTGTGAAATAGTTTTTGAAGATCCCAAGACACCAGG AGAAAAGCAGTTTGCATACCAGTGCTCCTGGGGCCTGACAACTCGCACTATCGGTGTGATGATCATGGTC CATGGGGACAACATGGGCTTAGTGTTACCTCCCCGTGTAGCGTCTGTCCAGGTGGTAGTCATTCCCTGTG GAATCACGAATACCTTGTCTGAAGAAGACAGAGAGGCCCTGATGGCAAAATGCAATGAGTACAGGAGACG GCTGCTCGGCGCTGACATCCGAGCTCGAGTCGATCTACGAGACAACTACTCACCAGGCTGGAAGTTCAAC CACTGGGAGCTCAAGGGTGTTCCAGTCAGACTTGAGGTGGGGCCACGTGATATGAAGAGCTGTCAGTTTG TAGCAGTCAGACGAGATACTGGAGAGAAGCTGACAATTGCTGAAAAGGAGGCGGAGTCTAAACTGAAAGG GGTTTTGGAAGACATCCAGCTTAACCTTTTCACAAGGGCTTCTGAAGACCTTAAATCTCACATGGTTGTA TCGGATACACTGGAAGACTTTCAGAAGGTGCTAGATTCTGGGAAGGTTGCACAGATTCCATTCTGTGGGG AAATTGACTGTGAGGACTGGATCAAAAAGACAACGGCCAGGGATCAGGATGTGGAACCTGGTGCTCCATC CATGGGAGCCAAAAGCCTTTGCATTCCTTTCAACCCTCTGTGTGAGCTGCAGCCAGGAGCCATGTGTGTC TGTGGCAAGAATCCTGCCAAGTTCTACACCTTGTTTGGTCGGAGTTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205417 representing NM_001024238 Red=Cloning site Green=Tags(s) MAALCLTVNAGNPPLGCRVKVNKRNKSFMLSFLEALLAVEHVKGDVSISVEEGKENLLRVSESVVFTDTN SILRYLARVAATSGLYGSNLMEHTEIDHWLEFSATKLSSCDVLTSAIKELNHCLSLRTYLVGNSLTLADL CVWATLKGNAACQEHLRQSKTLVHVNRWYGFLEAQQAFRSVGTKWDASANKATGAPDRKQDIGKFVELPG AEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIK PDQFTYTSDHFETIMKYAEKLIEEGKAYVDDTPADEMKAEPVEKNLQMWEEMKRGSQFGQSCCLRAKIDM SSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALG IRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVI PVNILDAQEEMKEVARHPKNPDVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNINITKIHKNPDGK ITSLDAKLNLENKDYKKTTKITWLAETTQALPVPAVCVTYEHLITKPVLGKDEDFKQYVNKDSKHEELML GDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCILIYIPDGHTKEMPTSGSKEKTKVEISKK ETGSAPKERPAAAVSAPCAAAEDAAILYSRVAVQGDAVRDLKAKKAPKEDIDAAVKQLLTLKAEYKEKTG QEYRPGNPPTAAVQTVSTKSPSNTGEYTSLYNKVAAQGELVRKLKAEKAPKAKINDAVECLLSLKAEYKE KTGKEYVPGQLPASQNSHSSPVSNAKPVGSETPEAKLLFDRVACQGEVVRKLKAEKASKDQVDSAVQELL QLKAQYKSLTGIEYKPVSATGAEDKDKKKKEKENKSEKQNKPQKQNDGQGKDSSKSQGSGLSSGGAGEGQ GPKKQTRLGLEAKKEENLAEWYSQVITKSEMIEYYDVSGCYILRPWSYSIWESIKDFFDAEIKKLGVENC YFPIFVSQGALEKEKNHIADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIRLNQ WCNVVRWEFKHPQPFLRTREFLWQEGHSAFATFEEAADEVLQILELYARVYEELLAIPVVRGRKTEKEKF AGGDYTTTLEAFISASGRAIQGATSHHLGQNFSKMCEIVFEDPKTPGEKQFAYQCSWGLTTRTIGVMIMV HGDNMGLVLPPRVASVQVVVIPCGITNTLSEEDREALMAKCNEYRRRLLGADIRARVDLRDNYSPGWKFN HWELKGVPVRLEVGPRDMKSCQFVAVRRDTGEKLTIAEKEAESKLKGVLEDIQLNLFTRASEDLKSHMVV SDTLEDFQKVLDSGKVAQIPFCGEIDCEDWIKKTTARDQDVEPGAPSMGAKSLCIPFNPLCELQPGAMCV CGKNPAKFYTLFGRSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001024238
ORF Size	4458 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001024238.1, NP_001019409.1
RefSeq Size	4461 bp
RefSeq ORF	4461 bp
Locus ID	289352
MW	166.8 kDa

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