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Krt17 (NM_212545) Rat Tagged ORF Clone
CAT＃: RR205536
- TrueORF®
Krt17 (Myc-DDK-tagged ORF) - Rat keratin 17 (Krt17), (10 ug)
"NM_212545" in other vectors (3)
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CNY 5,610.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ka17 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205536 representing NM_212545
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTACCACCATCCGCCAGTTCACGTCCTCCAGCTCCATCAAGGGCTCCTCTGGCCTGGGTGGCGGCT CATCTAGGACCTCCTGCCGACTGTCTGGCAGCCTGGGTGCAGGCTCCTGCAGGCTGGGGTCAGCTAGTGG CCTGGGCAGTGCTCTTGGGGGCAACAGTTACTCCAGCTGCTACAGCTTTGGTACTGGCAGTGGTTATGGA GGCAACTTTGGGGGTGTTGATGGGCTGCTGGCTGGAGGGGAGAAGGCCACCATGCAGAACCTCAACGACC GCCTGGCCTCCTACCTGGACAAGGTGCGTGCCCTGGAAGAGGCCAACACCGAGCTGGAGGTGAAGATCCG AGACTGGTACCAGAAGCAGGCCCCAGGGCCAGCCCGCGACTACAGTGCTTATTACCAGACCATTGAGGAT CTGAAGAACAAGATCCTCGTGGCCACTGTGGATAATGCCAGCATCCTACTCCAGATTGACAATGCCCGCT TGGCAGCTGATGACTTCCGTACCAAGTTTGAGACGGAGCAGGCCCTGCGCATGAGTGTGGAGGCGGACAT CAATGGCCTACGCCGGGTGCTGGATGAGCTGACCCTGGCCAGAGCTGACCTGGAGATGCAGATTGAGAAT CTCAAGGAGGAGCTGGCCTACCTGAAGAAGAACCATGAGGAGGAGATGAATGCTCTGAGAGGCCAGGTGG GCGGCGAAATCAACGTGGAGATGGACGCAGCCCCTGGTGTGGACCTGAGCCGCATCCTGTCAGAGATGCG GGATCAGTACGAGAAGATGGCTGAGAAGAACCGCAAGGATGCCGAAGATTGGTTCTTCAGCAAGACTGAG GAGCTAAACCGCGAGGTGGCCACCAACAGCGAGCTGGTGCAGAGCGGCAAGAGCGAGATCTCTGAGCTCC GGCGCACCATGCAGGCCCTGGAGATCGAGCTGCAGTCCCAGCTCAGCATGAAAGCATCCCTGGAGGGCAG CCTGGCAGAGACAGAGAACCGCTACTGCGTGCAGCTGTCCCAGATCCAGGGTTTGATTGGCAGCGTGGAG GAGCAGCTGGCTCAGCTGCGCTGCGAGATGGAGCAGCAGAATCAGGAGTACAAGATCCTGCTGGATGTGA AGACTCGGCTGGAGCAGGAGATCGCCACCTACCGCCGTCTGCTGGAGGGCGAGGATGCCCACCTGACTCA GTACAAGCCAAAAGAACCTGTGACCACCCGCCAGGTGCGCACCATTGTGGAAGAGGTCCAGGATGGCAAG GTCATCTCATCCCGGGAGCAGGTCCACCAGACCACCCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205536 representing NM_212545
Red=Cloning site Green=Tags(s) MTTTIRQFTSSSSIKGSSGLGGGSSRTSCRLSGSLGAGSCRLGSASGLGSALGGNSYSSCYSFGTGSGYG GNFGGVDGLLAGGEKATMQNLNDRLASYLDKVRALEEANTELEVKIRDWYQKQAPGPARDYSAYYQTIED LKNKILVATVDNASILLQIDNARLAADDFRTKFETEQALRMSVEADINGLRRVLDELTLARADLEMQIEN LKEELAYLKKNHEEEMNALRGQVGGEINVEMDAAPGVDLSRILSEMRDQYEKMAEKNRKDAEDWFFSKTE ELNREVATNSELVQSGKSEISELRRTMQALEIELQSQLSMKASLEGSLAETENRYCVQLSQIQGLIGSVE EQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKPKEPVTTRQVRTIVEEVQDGK VISSREQVHQTTR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_212545 |
| ORF Size | 1299 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_212545.2, NP_997710.1 |
| RefSeq Size | 1536 bp |
| RefSeq ORF | 1302 bp |
| Locus ID | 287702 |
| UniProt ID | Q6IFU8 |
| MW | 48.1 kDa |
| Gene Summary | Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin: promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors. May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205536 | Krt17 (untagged ORF) - Rat keratin 17 (Krt17), (10 ug) |
CNY 5,610.00 |
|
| RR205536L3 | Lenti ORF clone of Krt17 (Myc-DDK-tagged ORF) - Rat keratin 17 (Krt17), (10 ug) |
CNY 7,510.00 |
|
| RR205536L4 | Lenti ORF clone of Krt17 (mGFP-tagged ORF) - Rat keratin 17 (Krt17), (10 ug) |
CNY 8,270.00 |
