Izumo1 (NM_001017514) Rat Tagged Lenti ORF Clone
CAT#: RR206202L3
- LentiORF®
Lenti ORF clone of Izumo1 (Myc-DDK-tagged ORF) - Rat izumo sperm-egg fusion 1 (Izumo1), (10 ug)
"NM_001017514" in other vectors (3)
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CNY 6,080.00
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CNY 600.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RR206202).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001017514 |
ORF Size | 1149 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001017514.1, NP_001017514.1 |
RefSeq Size | 1503 bp |
RefSeq ORF | 1152 bp |
Locus ID | 499152 |
UniProt ID | Q6AY06 |
Gene Summary | Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN206202 | Izumo1 (untagged ORF) - Rat izumo sperm-egg fusion 1 (Izumo1), (10 ug) |
CNY 3,990.00 |
|
RR206202 | Izumo1 (Myc-DDK-tagged ORF) - Rat izumo sperm-egg fusion 1 (Izumo1), (10 ug) |
CNY 3,990.00 |
|
RR206202L4 | Lenti ORF clone of Izumo1 (mGFP-tagged ORF) - Rat izumo sperm-egg fusion 1 (Izumo1), (10 ug) |
CNY 6,650.00 |