Ank1 (NM_001107322) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206492 representing NM_001107322 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAACGGCCGCGCAGGTCGGGGTCAGACCCCGCGGCCGATGCTGCTACCAGCTTTTTGCGGGCGG CACGCTCAGGAAACCTGGACAAGGCTTTGGATCACCTACGCAATGGAGTGGACATTAACACCTGTAACCA GAACGGGTTGAATGGCTTGCATCTGGCCTCCAAAGAAGGCCATGTGAAGATGGTGGTTGAACTTCTGCAC AAAGAGATCATTCTAGAAACAACAACCAAGGTAAGGGATGGCTTCACTCCGCTGGCTGTGGCTCTGCAGC AGGGCCATGAGAATGTGGTGGCTCACCTCATCAACTATGGAACGAAAGGAAAAGTGCGCCTCCCTGCCCT GCACATCGCGGCCCGCAACGATGACACACGGACAGCCGCAGTCCTTCTGCAGAATGACCCCAACCCAGAT GTGCTTTCCAAGACGGGATTCACACCCCTCCACATCGCAGCACACTACGAGAACCTCAACGTGGCCCAGC TGCTCCTCAACCGGGGAGCCAGCGTCAACTTCACACCTCAGAATGGTATTACTCCACTGCACATCGCCTC CCGCAGGGGAAATGTGATCATGGTGAGACTCCTGCTGGACCGAGGGGCTCAGATAGAAACGAGGACCAAG GATGAACTGACACCACTCCACTGTGCAGCTCGGAATGGACACGTGAGAATCTCAGAGATCCTGCTGGACC ACGGGGCACCCATCCAAGCCAAAACCAAGAATGGCTTGTCCCCCATCCACATGGCGGCTCAGGGAGACCA CCTCGACTGTGTCCGACTTCTATTGCAATACAACGCCGAGATAGATGACATCACCTTAGATCACCTGACT CCTCTCCACGTGGCAGCCCACTGCGGCCACCACAGGGTGGCTAAGGTTCTTTTGGATAAAGGGGCCAAGC CGAACTCCAGAGCCCTGAATGGCTTTACCCCACTACACATCGCCTGCAAGAAGAACCACATACGTGTCAT GGAGCTGCTGCTGAAGACAGGAGCCTCTATTGACGCAGTCACTGAGAAAGTAGAAACCCCATTGCACATG GCAGCCCGAGCAGGGCATACAGAAGTGGCCAAATATTTGCTCCAGAACAAAGCCAAAGCCAATGCCAAGG CCAAGGACGACCAGACACCACTTCACTGTGCTGCTCGCATCGGCCACACGAGCATGGTGAAGCTCCTGCT GGAGAATGACGCCAGCCCCAACCTGGCTACCACTGCTGGCCACACACCTCTGCACACCGCAGCCCGTGAG GGTCACGTGGACACAGCCCTGGCCCTGCTGGAAAAGGAGGCATCCCAAGCCTGCATGACCAAGAAAGGAT TTACCCCTCTCCACGTGGCTGCCAAGTATGGGAAGGTCCGGCTGGCAGAGCTGCTGCTGGAACATGATGC CCATCCCAACGCGGCCGGAAAGAACGGCTTGACTCCCCTGCATGTGGCCGTCCATCACAACAACTTGGAC ATTGTCCAGCTTCTCCTTCCCCGAGGTGGCTCCCCGCACAGCCCTGCCTGGAATGGCTACACCCCTTTGC ACATCGCAGCCAAGCAGAACCAGATAGAGGTGGCCCGCAGTCTACTGCAATACGGAGGGTCCGCGAATGC AGAGTCTGTACAAGGAGTGACCCCGCTTCACCTGGCTGCCCAAGAGGGCCACACGGAAATGGTCGCTCTT CTCCTGTCAAAGCAAGCCAATGGCAACCTGGGGAACAAGAGTGGACTCACTCCTCTCCACCTGGTAGCAC AAGAAGGCCATGTTCCAGTGGCGGATGTGCTGATTAAACATGGCGTCACAGTGGATGCTACGACCCGGAT GGGTTACACCCCACTCCACGTGGCCAGTCACTACGGAAACATCAAGCTGGTGAAGTTCCTGCTACAGCAC CAGGCAGATGTCAACGCCAAGACCAAGCTAGGGTACAGCCCCTTGCACCAGGCAGCTCAGCAAGGACACA CGGACATTGTGACATTGCTCCTGAAGAATGGTGCTTCTCCAAATGAGGTCAGCTCGAACGGAACCACACC TCTGGCAATAGCCAAACGTTTGGGCTACATCTCTGTAACAGACGTGCTCAAGGTGGTCACAGATGAAACC AGTGTGGTGTTAGTCAGTGACAAACACCGGATGAGCTACCCTGAGACAGTGGATGAGATCCTGGATGTGT CTGAAGATGAAGGAACTGCTCACATATCTTTAACGGGTGACGAACTTGTCGGCTCGAAGGCTGAAAGGCG GGACTCCCGGGATGTGGGTGAGGAGAAAGAGCTGTTGGATTTTGTGCCGAAGCTAGACCAAGTGGTGGAA TCCCCAGCCATCCCAAGGATCCCTTGTGTCACGCCTGAGACTGTTGTGATCCGATCCGAAGATCAGGAAC AAGCATCTAAAGAGTATGATGAGGACTCACTCATTCCCAGTAGCCCAGCCACAGAGACCTCAGACAACAT CAGCCCCGTGGCTAGCCCCGTCCACACAGGGTTTCTGGTGAGCTTCATGGTCGATGCCCGGGGAGGATCC ATGAGAGGAAGTCGTCATAATGGCCTAAGGGTGGTGATCCCTCCCCGGACATGCGCAGCACCCACTCGCA TCACCTGCCGCCTGGTTAAGCCCCAGAAGCTAAGCACACCGCCCCCGCTGGCTGAGGAGGAGGGCCTAGC CAGCAGGATCATTGCACTGGGTCCTACGGGGGCCCAGTTCCTTAGCCCTGTGATCGTGGAAATCCCCCAC TTTGCCTCCCATGGCCGCGGGGACCGTGAGCTGGTGGTTCTGAGGAGCGAGAACGGCTCCGTGTGGAAGG AGCACAAGAGCCGCTATGGAGAAAGCTACCTGGACCAGATCCTGAACGGCATGGACGAAGAGCTGGGGAG CCTGGAGGAGCTGGAGAAGAAGCGCGTGTGTCGCATCATTACCACCGACTTCCCCCTGTACTTTGTCATC ATGTCCCGGCTCTGCCAGGACTACGACACCATCGGTCCTGAAGGGGGCTCCCTGAGAAGCAAGCTGGTGC CCCTGGTACAGGCGACGTTCCCCGAAAACGCGGTGACCAAGAAAGTGAAGCTGGCTCTGCAGGCCCAGCC TGTCCCAGACGAGCTGGTGACCAAGCTCCTGGGCAACCAGGCCACCTTCAGCCCCATTGTTACTGTTGAG CCAAGGCGTCGGAAGTTCCACCGTCCCATTGGGCTGCGAATCCCACTCCCTCCCTCATGGACTGACAACC CCAGGGACAGCGGGGAGGGAGACACCACCAGCCTACGCTTACTGTGCAGCGTCATCGGTGGAACTGACCA AGCCCAGTGGGAAGATATAACGGGAACAACCAAGCTTGTATACGCCAATGAGTGCGCCAACTTTACCACC AACGTCTCAGCCAGATTCTGGCTGTCGGACTGTCCTCGGACCGCTGAGGCCGTACACTTTGCCACTCTCT TGTACAAAGAGCTCACGGCAGTTCCCTACATGGCCAAGTTTGTCATTTTCGCCAAGATGAACGACCCCCG GGAAGGACGGCTACGCTGCTACTGCATGACAGATGACAAGGTGGACAAGACCCTGGAACAGCATGAAAAC TTCGTGGAGGTGGCCCGGAGCAGGGACATAGAGGTTCTAGAAGGGATGCCTCTGTTTGCAGAACTCTCTG GGAACTTGGTGCCTGTCAAGAAAGCGGCCCAGCAGCGAAGCTTCCATTTCCAGTCATTTCGAGAGAATCG CCTGGCCATCCCCGTGAAGGTGAGGGACAGCAGCCGCGAGCCAGGAGGGTTCTTGTCGTTCCTGCGGAAG CCGATGAAATATGAAGACGCACAGCACATCCTCTGCCACTTGAATGTCACCATGCCCCCCTGTACCAAGG GCAGTGGAGCAGAAGACAGGAGAAGGACCCTGACGCCCTTGACTCTTCGCTATAGCATTCTCAGCGAGTC CCGGCTGGGTTTTACCAGTGACACAGACCGTGTTGAAATGAAGATGGCTGTCATCAGGGAGCACCTCGGC CTCAGCTGGGCAGTGGAGAATCTGTACACGGCCCTGCGGAACATCGACAGAAGTGAGATTGTTAACATGT TGGAGGGCTCCGGCAGGCAGAGCAGAAACCTCAAACCAGACCGGAGGCATGGGGACCGGGAGTACTCATT GTCACCCTCCCAGGTGAATGGTTACTCCTCGCTGCAGGACGAGCTGCTGTCCCCCGCCTCCCTGCACTAC GCACTTCCCTCTCCACTGTGTGCAGACCAGTACTGGAACGAAGTGGCCGTCATAGACGCCATCCCCCTGG CGGCCACGGAGCATGACACCATGCTGGAGATGTCTGACATGCAGGTGTGGTCTTCGGGCCTCACACCCTC CCTGGTCACTGCTGAGGACTCCTCTCTGGAGTGCAGCAAGGCCGAGGACTCTGACGCCATACCAGAGTGG AAGTTGGAGGGGGCACACTCAGAGGACACGCAGGGCCCGGAGCTGGGCTCTCAGGACCTGGTGGAGGACG ACACAGTGGATTCAGATGCCACAAATGGCCTGGCAGATTTGCTAGGCCAGCAGCGAGTTCACGCCCGAAT CACAGACTCACCCTCAGTGAGGCAGGTGCTGGACAGAAGCCAGGCCAGAACAGTGGACTGGTATAAACAG GGTTCAGCAGCATCATATCCACAAGAGGCCGCACAAAGTTCCTGGCAAGAGGAGGTCACGCAAGGCCCAC AGTCATTCCAGAGAAGGATCACCACCATCCAAGGGCCGGAGCCCGGCACACTTCAGGAATATGAGCAGGT GGTGGTGTCCACCAGGGAGCATGTGCAGAGGGGGCCACCTGAGAGCGACAGCCCCAAGGCCGGGAAGGAC CCAAGTCTGTGGGCATCGGAGAGTGCCTTCTCTCAAGAGGTGCAGGGGGATGAGCTTCAGAATATTCCAG GAGAGCAGGTGACAGAGGAACAATTCACAGACGAACAGGGCAACATTGTCACCAAGAAGATCATTCGAAA AGTCGTCCGACAGGTGGACTCCTCTGGTGCCATCGACACCCAGCAGCACGAGGAGGTGGAGCTAAGAGGG AGTGGACTCCAGCCGGACCTGATAGAAGGCAGGAAGGGGGCTCAAATAGTGAAGCGGGCCAGCTTGAAAA GGGGCAAACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206492 representing NM_001107322 Red=Cloning site Green=Tags(s) MAERPRRSGSDPAADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLH KEIILETTTKVRDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPD VLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTK DELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLT PLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTEKVETPLHM AARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTSMVKLLLENDASPNLATTAGHTPLHTAARE GHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLD IVQLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVAL LLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQH QADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDET SVVLVSDKHRMSYPETVDEILDVSEDEGTAHISLTGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVE SPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGS MRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLSTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPH FASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVI MSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVE PRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLVYANECANFTT NVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDPREGRLRCYCMTDDKVDKTLEQHEN FVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRK PMKYEDAQHILCHLNVTMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMKMAVIREHLG LSWAVENLYTALRNIDRSEIVNMLEGSGRQSRNLKPDRRHGDREYSLSPSQVNGYSSLQDELLSPASLHY ALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDAIPEW KLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQQRVHARITDSPSVRQVLDRSQARTVDWYKQ GSAASYPQEAAQSSWQEEVTQGPQSFQRRITTIQGPEPGTLQEYEQVVVSTREHVQRGPPESDSPKAGKD PSLWASESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRG SGLQPDLIEGRKGAQIVKRASLKRGKQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107322
ORF Size	5121 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107322.2, NP_001100792.1
RefSeq Size	5124 bp
RefSeq ORF	5124 bp
Locus ID	306570
MW	187.1 kDa

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