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Klhl15 (NM_001108021) Rat Tagged ORF Clone
CAT＃: RR206896
- TrueORF®
Klhl15 (Myc-DDK-tagged ORF) - Rat kelch-like 15 (Drosophila) (Klhl15), (10 ug)
"NM_001108021" in other vectors (3)
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CNY 7,790.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RGD1563101 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206896 representing NM_001108021
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGGGACGTGGAAGGATTCTGTTCCTCCATCCATGACACCAGTGTCTCTGCTGGGTTCAGAGCAC TGTATGAGGAGGGATTGCTTCTTGATGTCACTCTGGTTATTGAAGACCATCAGTTCCAGGCCCATAAAGC CCTCCTGGCCACCCAGAGTGATTACTTCAGAATTATGTTCACAGCAGATATGAGGGAACGAGATCAGGAC AAAATTCATTTGAAGGGTCTAACAGCTACTGGTTTCAGCCACGTTCTTCAGTTTATGTATTATGGAACTA TAGAACTAAGTATGAATACTGTTCATGAAATCCTTCAGGCAGCAATGTATGTTCAGCTTATAGAAGTGGT GAAATTCTGCTGCTCTTTTCTCTTAGCCAAAATCTGCTTAGAAAATTGTGCGGAAATTATGAGACTGTTA GATGATTTTGGTGTTAACATCGAGGGAGTCAGGGAGAAGCTGGATGCCTTTCTGCTAGACAACTTCGTGC CACTCATGTCCAGGCCTGACTTCCTGTCTTATCTGAGCTTTGAGAAGCTCATGTCTTATTTGGATAATGA TCATCTGAGCAGGTTTCCAGAAATAGAGCTGTACGAGGCTGTGCAGTCTTGGCTGCGACATGATAGACGA CGGTGGAGACACACAGATACCATCATTCAGAACATCAGGTTTTGTCTGATGACTCCATCCAGCGTTTTTG AGAAGGTTAAAACATCAGAATTTTATAGATACTCCCGACAGCTGCGGTATGAAGTTGACCAAGCATTGAA TTACTTCCAAAATGTTCACCAGCAACCCTTGTTGGACATGAAATCAAGCCGTATTCGTTCTGCCAAACCC CAAACTACAGTATTCCGAGGAATGATTGGACATAGCATGGTTAATAGTAAAATACTCCTGTTAAAGAAAC CAAGAGTCTGGTGGGAACTGGAGGGCCCCCAAGTACCTCTAAGACCAGACTGCCTTGCCATTGTCAATAA TTTTGTGTTCCTGCTAGGTGGCGAAGAGCTGGGCCCCGATGGAGAATTCCATGCCTCTTCGAAAGTGTTC AGGTATGATCCAAGACAAAATTCTTGGCTGCGTATGGCAGACATGTCTGTACCACGTTCAGAATTTGCTG TTGGTGTCATTGGGAAGTTTATTTATGCTGTAGCAGGCAGAACAAGAGATGAAACTTTCTACTCAACAGA AAGATACGATATCACTAACGATAAATGGGAATTCGTAGATCCTTATCCAGTTAACAAATATGGACATGAG GGGACAGTGCTCAATAACAAGTTGTTTATCACTGGAGGAATCACTTCATCTTCCACATCTAAACAGGTGT GTGTATTTGACCCCAGTAAAGAAGGGACCATAGAACAGCGGACCAGGAGAACTCAAGTGGTTACCAACTG CTGGGAGAATAAAAGCAAGATGAATTATGCTAGATGCTTTCACAAGATGATCTCTTATAATGGCAAGCTT TATGTCTTTGGTGGTGTCTGTGTGATCTTGAGGGCCTCTTTTGAATCTCAGGGCTGCCCTTCCACAGAGG TGTATAACCCAGATACTGATCAATGGACCATCTTGGCATCTATGCCAATTGGTAGAAGTGGTCACGGTGT GACTGTGCTGGACAAACAAATCATGGTCCTTGGGGGCCTTTGTTATAATGGTCATTACAGTGATTCCATT CTCACCTTTGACCCAGATGAAAATAAGTGGAAAGAAGATGAGTACCCTCGGATGCCCTGCAAGCTGGATG GTTTACAGGTGTGCAACCTCCATTTTCCGGACTATGTACTGGATGAGGTTAGGCGTTGCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206896 representing NM_001108021
Red=Cloning site Green=Tags(s) MAGDVEGFCSSIHDTSVSAGFRALYEEGLLLDVTLVIEDHQFQAHKALLATQSDYFRIMFTADMRERDQD KIHLKGLTATGFSHVLQFMYYGTIELSMNTVHEILQAAMYVQLIEVVKFCCSFLLAKICLENCAEIMRLL DDFGVNIEGVREKLDAFLLDNFVPLMSRPDFLSYLSFEKLMSYLDNDHLSRFPEIELYEAVQSWLRHDRR RWRHTDTIIQNIRFCLMTPSSVFEKVKTSEFYRYSRQLRYEVDQALNYFQNVHQQPLLDMKSSRIRSAKP QTTVFRGMIGHSMVNSKILLLKKPRVWWELEGPQVPLRPDCLAIVNNFVFLLGGEELGPDGEFHASSKVF RYDPRQNSWLRMADMSVPRSEFAVGVIGKFIYAVAGRTRDETFYSTERYDITNDKWEFVDPYPVNKYGHE GTVLNNKLFITGGITSSSTSKQVCVFDPSKEGTIEQRTRRTQVVTNCWENKSKMNYARCFHKMISYNGKL YVFGGVCVILRASFESQGCPSTEVYNPDTDQWTILASMPIGRSGHGVTVLDKQIMVLGGLCYNGHYSDSI LTFDPDENKWKEDEYPRMPCKLDGLQVCNLHFPDYVLDEVRRCN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001108021 |
| ORF Size | 1812 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001108021.1, NP_001101491.1 |
| RefSeq Size | 1969 bp |
| RefSeq ORF | 1815 bp |
| Locus ID | 314111 |
| UniProt ID | D3ZA50 |
| MW | 69.7 kDa |
| Gene Summary | Substrate-specific adapter for CUL3 E3 ubiquitin-protein ligase complex. Acts as an adapter for CUL3 to target the serine/threonine-protein phosphatase 2A (PP2A) subunit PPP2R5B for ubiquitination and subsequent proteasomal degradation, thus promoting exchange with other regulatory subunits and regulating PP2A holoenzyme composition. Acts as an adapter for CUL3 to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, plays a key role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206896 | Klhl15 (untagged ORF) - Rat kelch-like 15 (Drosophila) (Klhl15), (10 ug) |
CNY 7,790.00 |
|
| RR206896L3 | Lenti ORF clone of Klhl15 (Myc-DDK-tagged ORF) - Rat kelch-like 15 (Drosophila) (Klhl15), (10 ug) |
CNY 9,600.00 |
|
| RR206896L4 | Lenti ORF clone of Klhl15 (mGFP-tagged ORF) - Rat kelch-like 15 (Drosophila) (Klhl15), (10 ug) |
CNY 10,550.00 |
