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Rapgef3 (NM_021690) Rat Tagged ORF Clone
CAT＃: RR207125
- TrueORF®
Rapgef3 (Myc-DDK-tagged ORF) - Rat Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), (10 ug)
"NM_021690" in other vectors (3)
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CNY 11,310.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Epac |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR207125 representing NM_021690
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGAAGAGAATGCACCGTCCCCGGTGCTGCTCTTACCAGCTAGTGTTCGAGCACCGGCGCCCAA GCTGCATCCAGGGACTTCGCTGGACGCCACTTACCAACAGTGAGGGCTCCCTGGACTTCAGAGTGAGCCT GGAGCAGGCCACCACAGAGCATGTGCACAAGGCCGGGAAGCTCCTGTACCGTCATCTCTTGGCAACGTAC CCTACCCTCATCCGAGACAGAAAATACCATCTGCGACTACATCGGCAGTGCTGCTCTGGCCGGGAGCTAG TGGATGGGATCTTGGCTCTGGGTCTTGGGGTCCACTCACGGAGCCAAGCTGTGGGCATCTGCCAGGTGTT GCTGGATGAGGGTGCCCTTTGCCATGTAAAACATGACTGGACCTTCCAGGACCGAGACGCCCAATTCTAC AGATTCCCTGGACCGGAGCCCCAGCCTGCAGGAACTCATGACGTGGAAGAGGAGCTTGTTGAGGCAATGG CCCTACTCTCCCAGCGAGGGCCTGATGCCCTACTCACTGTTGCACTCCGGAAGTCCCCGGGTCAGCGTAC AGATGAAGAGCTGGACCTCATCTTCGAGGAGCTCGTACATATCAAGGCGGTGGCTCACCTTTCTAACTCG GTGAAACGGGAACTAGCTGCTGTTCTGCTCTTTGAACCACACAGCAAGGCAGGAACTGTGTTGTTCAGCC AGGGGGACAAGGGTACCTCATGGTACATTATCTGGAAGGGATCTGTCAATGTGGTGACCCGTGGCAAGGG GCTGGTGACCACGTTGCACGAGGGAGATGACTTTGGACAGCTGGCTCTGGTGAACGACGCACCTCGAGCA GCCACCATCATCCTTCGAGAAAATAACTGTCACTTTCTGCGTGTGGACAAGCAGGACTTCAACCGCATCA TCAAGGATGTGGAAGCAAAAACCATGAGACTGGAAGAACACGGCAAAGTGGTGTTAGTTTTGGAGAGAAC CTCTCAGGGTGCTGGCCCTTCCCGCCCTCCGACCCCAGGCAGGAACCGATATACGGTAATGTCTGGCACC CCAGAGAAAATCCTAGAACTCCTGTTGGAGGCTATGAGACCGGATTCCAGTGCTCATGACCCAACAGAGA CATTCCTCAGTGACTTCCTGCTGACGCACAGTGTCTTCATGCCCTGCACACAGCTCTTTGCCGCCCTCCT GCACCACTTCCACGTGGAGCCATCAGAGCCTGCCGGGGGCAGCGAGCAGGAACGCAGCACCTACATCTGC AACAAGAGGCAGCAGATTCTGCGTCTGGTCAGCCGGTGGGTGGCCCTCTACAGCCCCATGCTCCGCTCAG ATCCCGTGGCCACCAGCTTCCTCCAGAAACTCTCAGACCTGGTGAGCAGAGATACCCGACTTAGCAACTT GCTGAGGGAACAGTATCCGGAGAGACGGCGACACCACAGGTTGGAGAATGGCTGTGGGAATGTATCTCCT CAGACCAAGGCCCGGAATGCACCTGTTTGGTTTCCTAACCATGAGGAACCCCTCCCAAGCAGCGCTGGGG CCATCCGAGTCGGGGACAAAGTCCCCTATGATATCTGCAGACCCGACCACTCGGTGCTGACCCTGCACCT GCCGGTGACAGCCTCCGTGAGGGAAGTGATGGCAGCTTTGGCCCATGAGGACCACTGGACCAAGGGGCAG GTGCTGGTAAAGGTCAATTCTGCCGGTGATGTCGTTGGCTTGCAGCCAGATGCCCGCGGTGTGGCCACAT CCCTGGGGCTCAATGAGCGGATCTTTGTTGTCGACCCACAGGAAGTGCACGAGCTGACCCCACACCCTGA GCAGCTGGGGCCCACTCTGGGTTCTTCTGAGATGCTGGACCTAGTGAGTGCCAAGGACCTGGCAGGCCAG CTCACAGAGCATGACTGGAACCTCTTCAACAGGATCCACCAGGTGGAGCTGATCCACTATGTACTGGGCC CCCAGCACCTGCGGGACGTCACCACTGCAAACCTGGAGCGCTTCATGCGACGCTTCAACGAGCTGCAGTA CTGGGTGGCCACCGAACTCTGTCTCTGCCCGGTTCCTGGCCCCCGGGCTCAGCTACTCCGGAAGTTCATC AAGCTGGCAGCCCACCTCAAGGAGCAGAAGAATCTCAACTCTTTCTTTGCGGTCATGTTTGGCCTCAGCA ACTCGGCCATCAGCCGCCTGGCCCACACCTGGGAGCGTCTGCCCCATAAAGTACGGAAGCTGTACTCGGC CCTGGAAAGGTTGCTGGACCCTTCCTGGAACCACCGAGTGTACCGATTGGCTCTCACCAAGCTCTCTCCT CCTGTCATCCCTTTCATGCCCCTGCTACTCAAAGACATGACCTTCATTCATGAAGGGAACCACACACTGG TAGAAAACCTCATCAACTTTGAGAAGATGCGAATGATGGCCAGAGCCGTGCGGATGCTCCACCACTGCCG AAGCCACAGCACCGCGCCTCTATCACCACTCAGAAGCCGGGTTTCCCACATCCACGAGGACAGCCAGGCA TCAAGAATCTCCACATGTTCCGAGCAGTCCCTGAGCACCCGGAGTCCAGCCAGCACCTGGGCTTATGTCC AGCAGCTGAAGGTCATTGACAACCAGCGGGAACTGTCCCGCCTCTCCCGGGAACTGGAACCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207125 representing NM_021690
Red=Cloning site Green=Tags(s) MVLKRMHRPRCCSYQLVFEHRRPSCIQGLRWTPLTNSEGSLDFRVSLEQATTEHVHKAGKLLYRHLLATY PTLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDAQFY RFPGPEPQPAGTHDVEEELVEAMALLSQRGPDALLTVALRKSPGQRTDEELDLIFEELVHIKAVAHLSNS VKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTRGKGLVTTLHEGDDFGQLALVNDAPRA ATIILRENNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGAGPSRPPTPGRNRYTVMSGT PEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPCTQLFAALLHHFHVEPSEPAGGSEQERSTYIC NKRQQILRLVSRWVALYSPMLRSDPVATSFLQKLSDLVSRDTRLSNLLREQYPERRRHHRLENGCGNVSP QTKARNAPVWFPNHEEPLPSSAGAIRVGDKVPYDICRPDHSVLTLHLPVTASVREVMAALAHEDHWTKGQ VLVKVNSAGDVVGLQPDARGVATSLGLNERIFVVDPQEVHELTPHPEQLGPTLGSSEMLDLVSAKDLAGQ LTEHDWNLFNRIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFI KLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSP PVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAVRMLHHCRSHSTAPLSPLRSRVSHIHEDSQA SRISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021690 |
| ORF Size | 2652 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021690.1, NP_067722.1 |
| RefSeq Size | 3373 bp |
| RefSeq ORF | 2655 bp |
| Locus ID | 59326 |
| UniProt ID | Q9Z1C8 |
| MW | 100.3 kDa |
| Gene Summary | binds cAMP and activates the Ras superfamily guanine nucleotide binding protein (Rap1A)in a PKA-independent manner [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN207125 | Rapgef3 (untagged ORF) - Rat Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), (10 ug) |
CNY 11,310.00 |
|
| RR207125L3 | Lenti ORF clone of Rapgef3 (Myc-DDK-tagged ORF) - Rat Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), (10 ug) |
CNY 13,110.00 |
|
| RR207125L4 | Lenti ORF clone of Rapgef3 (mGFP-tagged ORF) - Rat Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), (10 ug) |
CNY 14,350.00 |
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