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Emd (NM_012948) Rat Tagged ORF Clone
CAT＃: RR208987
- TrueORF®
Emd (Myc-DDK-tagged ORF) - Rat emerin (Emd), (10 ug)
"NM_012948" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR208987 representing NM_012948
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGACTATGCGGTTTTGTCCGATACTGAGCTGGCCGCAGTGCTACGCCAGTACAACATCCCGCATG GGCCTATTCTGGGCTCCACTCGCAAGCTCTACGAAAAGAAAATCTTCGAGTACGAGACCCAGAGAAGGAG GCTTTCGCCCCCCAGCTCGTCATCGTCTTCATTCTCCTATCGGTTCTCAGACTTGGATTCAGCCTCCGTG GACTCAGACATGTATGATCTGCCCAAAAAGGAGGACGCCTTACTTTACCAGAGCAAGGACTATAATGATG ACTACTATGAGGAGAGCTATTTGACTACCAGGACATACGGGGAGCCCGAGTCTGTGGGCATGTCCAAGAG CTTCCGCCGGCCAGGGACCTCACTTGTAGATGCTGATGATACCTTCCATCACCAGGTGCGTGATGACATT TTCTCTTCTTCAGAAGAGGAAGGCAAGGATAGGGAACGCCCCATCTATGGCCGAGACAGTGCCTACCAGA GCATCGCAGAGTACCGCCCCATTTCCAATGTCTCCAGGAGCTCCCTGGGCTTATCATATTATCCTAGATC CTCCACCTCTTCTGTGTCCTCCTCTTCATCTTCTCCCTCTTCATGGCTTACCCGACGTGCCATTCGACCA GAAAAGCAGGCCCCTACTGCTGCTCTGGGCCAGGATCGACAGGTCCCACTCTGGGGCCAGCTGTTACTCT TCCTGGCCTTTGCTACCTTTCTGCTCTTTGTTTACTATTCCATACAAGCTCAAGAAGGCAACCCCTTCTG GATGGATCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208987 representing NM_012948
Red=Cloning site Green=Tags(s) MDDYAVLSDTELAAVLRQYNIPHGPILGSTRKLYEKKIFEYETQRRRLSPPSSSSSSFSYRFSDLDSASV DSDMYDLPKKEDALLYQSKDYNDDYYEESYLTTRTYGEPESVGMSKSFRRPGTSLVDADDTFHHQVRDDI FSSSEEEGKDRERPIYGRDSAYQSIAEYRPISNVSRSSLGLSYYPRSSTSSVSSSSSSPSSWLTRRAIRP EKQAPTAALGQDRQVPLWGQLLLFLAFATFLLFVYYSIQAQEGNPFWMDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012948 |
| ORF Size | 780 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012948.1, NP_037080.1 |
| RefSeq Size | 1274 bp |
| RefSeq ORF | 783 bp |
| Locus ID | 25437 |
| UniProt ID | Q63190 |
| MW | 29.7 kDa |
| Gene Summary | Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. Required for proper localization of non-farnesylated prelamin-A/C (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN208987 | Emd (untagged ORF) - Rat emerin (Emd), (10 ug) |
CNY 3,990.00 |
|
| RR208987L3 | Lenti ORF clone of Emd (Myc-DDK-tagged ORF) - Rat emerin (Emd), (10 ug) |
CNY 6,080.00 |
|
| RR208987L4 | Lenti ORF clone of Emd (mGFP-tagged ORF) - Rat emerin (Emd), (10 ug) |
CNY 6,650.00 |
