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Prkra (NM_001024780) Rat Tagged ORF Clone
CAT＃: RR209032
- TrueORF®
Prkra (Myc-DDK-tagged ORF) - Rat protein kinase, interferon inducible double stranded RNA dependent activator (Prkra), (10 ug)
"NM_001024780" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RAX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR209032 representing NM_001024780
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCACAGCAGGCATCGTGCCGAGGCCCCGCCGCTGCAGCGCGAGGACAGCGGGACCTTCAGTTTGG GCAAGATGATAACAGCTAAGCCTGGGAAAACACCGATTCAGGTATTGCACGAGTACGGCACGAAGACCAA GAACATTCCAGTTTATGAATGTGAAAGATCCGATGTGCAAGTACACGTGCCCACTTTCACCTTCAGAGTA ACCGTTGGTGACATAACTTGCACAGGTGAAGGGACGAGTAAGAAGCTGGCGAAGCACAGAGCTGCAGAGG CTGCCATAAACATTTTGAAAGCCAATGCAAGTATTTGCTTTGCAGTTCCTGACCCCTTAATGCCTGATCC ATCCAAACAGCCAAAGAACCAGCTGAATCCAATTGGCTCATTACAGGAATTAGCAATTCACCATGGCTGG CGGCTTCCTGAATATACCCTTTCCCAGGAAGGAGGACCCGCTCATAAGAGAGAATATACCACGATTTGCA GGCTAGAGTCATTCATGGAAACTGGAAAGGGGGCATCAAAAAAACAAGCAAAGAGAAACGCTGCTGAGAA ATTCCTCGCCAAATTTAGTAATATTTCTCCAGAGAACCACATTTCTCTAACGAATGTGGTCGGACATTCC CTGGGATGCACTTGGCACTCCTTGAGGAATTCCCCTGGTGAAAAGATCAACCTTCTGAAAAGGAGCCTCC TCAGTCTGCCCAACACAGACTACATCCAGCTGCTCAGTGAGATAGCCAAAGAGCAAGGCTTCAGTATAAC ATACTTGGATATAGAGGAGCTGAGTGCCAACGGGCAGTATCAGTGTCTCGCAGAGCTGTCCACCAGTCCC ATCACCGTGTGTCATGGCTCAGGCATCTCCTGTGGCAATGCCCAGAGTGACGCCGCTCACAATGCCCTGC AGTATCTAAAGATAATAGCAGAAAGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209032 representing NM_001024780
Red=Cloning site Green=Tags(s) MSHSRHRAEAPPLQREDSGTFSLGKMITAKPGKTPIQVLHEYGTKTKNIPVYECERSDVQVHVPTFTFRV TVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGW RLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHS LGCTWHSLRNSPGEKINLLKRSLLSLPNTDYIQLLSEIAKEQGFSITYLDIEELSANGQYQCLAELSTSP ITVCHGSGISCGNAQSDAAHNALQYLKIIAERK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001024780 |
| ORF Size | 939 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001024780.1, NP_001019951.1 |
| RefSeq Size | 1589 bp |
| RefSeq ORF | 942 bp |
| Locus ID | 311130 |
| UniProt ID | Q4V8C7 |
| MW | 34.4 kDa |
| Gene Summary | Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association, sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN209032 | Prkra (untagged ORF) - Rat protein kinase, interferon inducible double stranded RNA dependent activator (Prkra), (10 ug) |
CNY 3,990.00 |
|
| RR209032L3 | Lenti ORF clone of Prkra (Myc-DDK-tagged ORF) - Rat protein kinase, interferon inducible double stranded RNA dependent activator (Prkra), (10 ug) |
CNY 6,080.00 |
|
| RR209032L4 | Lenti ORF clone of Prkra (mGFP-tagged ORF) - Rat protein kinase, interferon inducible double stranded RNA dependent activator (Prkra), (10 ug) |
CNY 6,650.00 |
