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Spi1 (NM_001005892) Rat Tagged ORF Clone
CAT＃: RR210022
- TrueORF®
Spi1 (Myc-DDK-tagged ORF) - Rat SFFV proviral integration 1 (Sfpi1), (10 ug)
"NM_001005892" in other vectors (3)
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CNY 3,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Pu.1; Sfpi1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210022 representing NM_001005892
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTACAGGCGTGCAAAATGGAAGGGTTTCCCCTCGTCGCCCCTCCATCGGATGAGCTGGTTACTTATG AATCAGAACTATACCAACGTCAAACACATGACTACTATTCCTTGATTGGTGGTGATGGAGACAGCCATAG CGATCACTATTGGGATTTCTCCACACACCATGTACACAGCGAGTTTGAGAGCTTCCCTGAGAACCACTTC ACGGAGCTGCAGAGCGTGCAGCCCCCGCAGCTACAGCAGCTCTACCGCCACATGGAGCTGGAGCAGATGC ACGTCCTTGACACCCCCATGGCACCGCCCCATGCCAGCCTCAGTCACCAGGTTTCCTACATGCCCCGGGT GTGCTTCCCTTATCCACCCTTGTCCCCAGCCCACCAGCAGAGCTCAGATGAGGAGGAGGGTGAGAGGCAG AGCCCTCCACTGGAGGTGTCTGATGGAGAAGCTGATGGCTTGGAGCCTGGGCCAGGCCTTCTGCATGGGG AGACAGGCAGCAAGAAGAAGATTCGTTTGTACCAGTTCCTGCTGGACCTGCTGCGTAGCGGAGACATGAA GGACAGCATCTGGTGGGTGGACAAGGACAAAGGTACCTTCCAGTTCTCGTCCAAGCACAAGGAGGCGCTG GCGCACCGCTGGGGCATCCAGAAGGGAAACCGCAAGAAGATGACCTACCAGAAGATGGCGCGCGCGCTGC GCAACTACGGCAAGACGGGCGAGGTGAAGAAAGTCAAGAAGAAGCTCACTTACCAGTTCAGCGGCGAGGT GCTGGGCCGTGGGGGCCTGGCTGAGCGGCGCCTCCCGCCCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210022 representing NM_001005892
Red=Cloning site Green=Tags(s) MLQACKMEGFPLVAPPSDELVTYESELYQRQTHDYYSLIGGDGDSHSDHYWDFSTHHVHSEFESFPENHF TELQSVQPPQLQQLYRHMELEQMHVLDTPMAPPHASLSHQVSYMPRVCFPYPPLSPAHQQSSDEEEGERQ SPPLEVSDGEADGLEPGPGLLHGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEAL AHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRLPPH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001005892 |
| ORF Size | 813 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001005892.2, NP_001005892.1 |
| RefSeq Size | 1373 bp |
| RefSeq ORF | 816 bp |
| Locus ID | 366126 |
| UniProt ID | Q6BDS1 |
| MW | 31.5 kDa |
| Gene Summary | Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210022 | Spi1 (untagged ORF) - Rat SFFV proviral integration 1 (Sfpi1), (10 ug) |
CNY 3,990.00 |
|
| RR210022L3 | Lenti ORF clone of Spi1 (Myc-DDK-tagged ORF) - Rat SFFV proviral integration 1 (Sfpi1), (10 ug) |
CNY 6,080.00 |
|
| RR210022L4 | Lenti ORF clone of Spi1 (mGFP-tagged ORF) - Rat SFFV proviral integration 1 (Sfpi1), (10 ug) |
CNY 6,650.00 |
