Pim1 (NM_017034) Rat Tagged ORF Clone
CAT#: RR210111
- TrueORF®
Pim1 (Myc-DDK-tagged ORF) - Rat pim-1 oncogene (Pim1), (10 ug)
"NM_017034" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR210111 representing NM_017034
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTTGTCCAAGATCAACTCCCTGGCCCACCTGCGCGCAGCCCCTTGCAACGACCTGCACGCCAACA AGCTGGCGCCGGGCAAAGAGAAGGAGCCCCTGGAGTCGCAGTACCAGGTGGGCCCGCTGTTGGGCAGCGG TGGCTTCGGCTCGGTCTACTCGGGCATCCGCGTCGCCGACAACTTGCCGGTGGCCATCAAGCACGTGGAG AAGGACCGGATTTCCGACTGGGGGGAACTGCCCAACGGCACCCGAGTGCCCATGGAAGTGGTCCTGCTGA AGAAGGTGAGCTCGGGCTTCTCGGGCGTCATTAGACTTCTGGACTGGTTCGAGAGGCCCGATAGTTTCGT GCTGATCCTGGAGAGGCCCGAACCCGTGCAAGACCTCTTCGACTTCATCACCGAGCGAGGAGCCCTCCAG GAGGAGCTGGCCCGGAGCTTCTTCTGGCAGGTGCTGGAGGCCGTGCGGCATTGCCACAACTGCGGGGTTC TCCACCGCGACATCAAGGACGAGAACATCTTAATCGACCTGAACCGCGGCGAACTCAAACTCATCGACTT CGGGTCGGGGGCGCTGCTCAAGGACACAGTCTACACGGACTTTGACGGAACCCGAGTGTACAGTCCTCCA GAGTGGATTCGCTACCATCGCTACCACGGCAGGTCGGCTGCTGTTTGGTCCCTGGGGATCCTGCTCTATG ACATGGTCTGCGGAGATATTCCATTTGAGCACGACGAAGAGATCGTCAAGGGCCAAGTGTACTTTAGGCA AAGGGTCTCTTCAGAATGTCAACATCTTATTAGATGGTGCCTGTCCCTGAGACCATCGGACCGGCCCTCC TTTGAAGAAATCCAGAACCATCCGTGGATGCAGGATGTTCTCCTGCCCCAGGCCACCGCCGAGATTCATC TGCACAGCCTGTCACCATCACCCAGCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210111 representing NM_017034
Red=Cloning site Green=Tags(s) MLLSKINSLAHLRAAPCNDLHANKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVADNLPVAIKHVE KDRISDWGELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERPDSFVLILERPEPVQDLFDFITERGALQ EELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPP EWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIVKGQVYFRQRVSSECQHLIRWCLSLRPSDRPS FEEIQNHPWMQDVLLPQATAEIHLHSLSPSPSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_017034 |
ORF Size | 939 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017034.1, NP_058730.1 |
RefSeq Size | 1302 bp |
RefSeq ORF | 942 bp |
Locus ID | 24649 |
UniProt ID | P26794 |
MW | 35.6 kDa |
Gene Summary | serine threonine protein kinase; 2.8 kb transcript is detected in somatic cells and a 2.3 kb transcript using an alternate polyadenylation signal is expressed in testis [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN210111 | Pim1 (untagged ORF) - Rat pim-1 oncogene (Pim1), (10 ug) |
CNY 3,990.00 |
|
RR210111L3 | Lenti ORF clone of Pim1 (Myc-DDK-tagged ORF) - Rat pim-1 oncogene (Pim1), (10 ug) |
CNY 6,080.00 |
|
RR210111L4 | Lenti ORF clone of Pim1 (mGFP-tagged ORF) - Rat pim-1 oncogene (Pim1), (10 ug) |
CNY 6,650.00 |