Rb1cc1 (NM_001107901) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210423 representing NM_001107901 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTATATGTGTTTCTGGTTAACACTGGAACCACTCTGACATTTGACACTGAGCTAACTGTGCAAA CTGTGGCTGATCTGAAGCATGCCATTCAAAGCAAATACAAGATTGCTGTTCAGCACCAGGTGCTAGTTGT CAATGGAGGAGAATGCATGACTGCAGATCGAAGAGTGTGCACTTACAGCGCTGGGACGGACACAAATCCA ATTTTTCTTTTTAATAAAGAAATGATCTTATGTGACCGTGCACCTGCTATTCCTAAAACCACCTTTTCAA CAGAAAATGACATGGAAATAAAAGTCGAAGAGTCTCTTATGATGCCTGCAGTTTTTCACACTGTCGCTTC AAGGACACAGCTTGCAGTGGAAATGTATGAAGTTGCCAAGAAGCTTTGCTCTTTCTGTGAAGGCCTTGTC CATGATGAACATCTTCAGCACCAAGGCTGGGCTGCAATCATGGCCAATCTGGAGGACTGTTCAACTTCAT ACCAAAAACTCCTTTTCAAGTTTGAAAGTATTTATTCTGATTATCTGCAATCCATAGAAGACATCAAGTT AAAACTTACTCATTTAGGAACTGCGGTTTCAGTAATGGCCAAGATTCCACTGTTGGAGTGCCTAACCAGA CATAGTTACAGGGAATGTTTGGGAAGACCGGATTCTTTAAATGAACATGAAGGCTCAGAGAAAGCTGATA TGAAAAGATCTACTGAACTGGTGCTCTCTCCTGATATGCCTAGAACAACTAACACATCCTTGTTAACCTC ATTTCACAAGTCAGTGGAGCATGTAGCTCCAGATGTCACTGATGCTGAACAAGGCAAAGAACTTCGAGAA TCTTGTCAAAGTACTGTCCAGCAAGAAGACGCTCCAGTAGATGCTAAAGACAGTGATCTGCCTTTTTTTA ATGTTTCTTTGTTAGACTGGATAAATGTTCAAGATAGACCTAATGATGTGGAATCTCTGGTCAGGAAGTG CTTTGATTCTATGAGCAGGCTTGACCCAAAGATTATTCAACCGTTTATGTTAGAATGCCATCAAACTATT GCCAAACTTGATAATCAGAATATGAAAGCCATTAAAGGACTTGAAGATCGGCTGTATGCCTTGGACCAGA TGATTGCTAGCTGTAGCAGGCTGGTGAATGAACAGAAAGAGCTTGCTCAGGGATTTTTAGCTAATCAGAT GAGAGCAGAAAACTTGAAGGATGCATCTGTGCTACCTGATCTGTGCCTGAGTCATGCAAATCAGCTAATG ATTATGTTGCAAAACCACAGAAAACTGTTGGACATTAAACAGAAGTGTACCACTGCCAAACAAGAGCTAG CAAACAATCTGCATGTCAGACTGAAGTGGTGTTGTTTTGTGATGCTTCATGCTGATCAAGATGGAGAAAA ACTGCAAGCACTACTCCGCCTTGTAATAGAGCTATTAGAAAGAGTCAGAATTGTTGAGGCTCTTAGTACA GTTCCTCAGATGTACTGCCTAGCTGTTGTTGAGGTTGTAAGGAGAAAAATGTTCATTAAACACTACAGAG AGTGGGCCGGTGCTTTAGTCAAAGATGGAAAACAATTGTATGAAGCTGAAAAGTCAAAAAGGGAGTCCTT TGGGAAATTATTTAGGAAGTCCTTTTTAAGAAATCGTCTGTTTAAGGGACTGGACTCCTGGCCTTCCTCA TTTTGTACTCAGAAGCCTCGAAAATTTGACTGTGAACTTCCAGATATATCATTAAAAGATTTACAGTTTC TTCAATCATTTTGTCCTTCAGAAGTGCAGCCATTCCTCAGGGTTCCTTTACTTTGTGACTTTGAACCTCT ACACCAGCATGTACTTGCCCTACATAATTTGGTAAAAGCAGCACAAAGTTTGGATGAAATGTCACAGACC ATTACAGATCTGCTGAATGAACAAAAGGTATCCACAAGTCAAGCATCTCCACAGTCAGCTGCTTCTCCAA GAATAGAAAGTACAACAGGCATTACAACTACTACCTCACCAAAAGCTCCTCCTCCACTAACTGTTCAGGA TACTCTATATCCGGCAGCGTGTCCCTTAGAAGAGCTGTCTCCGGATAGCATCGATGCTCACACATTTGAT TTTGAAACCGTCTCCCATCCAAACACAGAGCAAACAGTTCACCAGGCTTCTATAGACTTGGACTCATTAG CAGAAAGCCCTGAGTCTGATTTTATGTCTGCTGTGAATGAGTTTGTGATAGAAGAAAATTTATCATCTCC GAACCCTATAAGTGATCCACAAAGTCCAGAAATGATGGTGGAGTCGCTTTACTCTTCAGTCATCAATGCA ATAGATAGTAGGCGCATGCAGGACACAAGCACACGTGGAAACGAGGGCTTTGGGGATCGCGCTGCGCTAC ATGTCCAGCTCGAGAAATGCAGAGTTGCTGCCCAAGACTCTCACATCAGTATACAAACCATCAAGGATGA TCTGTGCCACTTCAGAACATTTGTACAGAAAGAACAGTGTGACTTATCAAATTATTTAAAATGTACAGCT GTAGAAATAAGAAATATTATTGACAAAGTAAAATGTTCCCTAGAAATAACACTAAAGGAAAAACATCAGC AAGAACTCCAATCTTTAAAAATTGAGTATGAATGTAAACTTAATACTCTAGAAAAAGACAGTGAAGAAAA TGTAAATAAAATTTTAAAATTGAAAGAAGATTTAGTATCCCTTGAGGAGGCTTTACAAAATAAAGACAGT GAATTTGCGATAATTAAGCATGAAAAGGATGCTATTGTCTGCATGCAGCATGAGAAGGATCAGAAGTTGT TAGAGATGGAAAATATAATGCATACTCAAAACAGTGAAATTAAAGAACTGAAGCAGTCACGAGAAATGGT GTTAGAAGACCTGAAAAAACTGCATGATGAAAAAATAGAGTCATTGAGAGCTGAATTTCAGTGCCTAGAA CAAAATCACTTGAAGGAATTAGAAGACACACTGCACATCAGGCACACAGAGGAGTTTGAGAAAGTTATAA CAGACCACAAAGTATCTTTGGAGAAATTAAAAAAGGAAAATCAGCAAAAAATTGACCAGATGCTAGAATC TCATGCCTCAGCTATTCAGGAGAAAGAGCAGCAGCTGCAGGAATTGAAAGTCAAGGTTTCTGACTTGTCA GACATGAGGTGTAAGTTAGAGGTTGAACTTGCACTGAAGGAAGCAGAAACAGATGAAATAAAGATCTTGC TGGAAGAGAGCAGAACACAGCAGAAGGAAACACTGAAGTCTCTACTTGAACAAGAAACCGAAAACTTAAG AACAGAAATAAATAAATTAAACCAAAAAATTCATGATAATAGTGAGAGTTATCAGGTGGGTTTATCAGAG TTAAGATCTTTAATGACAATTGAAAAAGATCAGTGCATTTCAGAGTTAATCAGTAGACATGAGGAAGAAT CTAATATACTTAAGGCTGAATTAGACAGTGTTACAGCTTTGCATCACCAAGCATTTGAAATAGAAAAAAA ACTGAAAGAACAAATAGTTGAATTGCAGACTAAATTGAACTCAGAACTGAGTGCTCTTGAAAAACAGAAA GATGAAAAAATAACCCAACAAGAAGAGAAGTATAAAGCTCTTATACAGAACCTTGAGAAAGACAAGCAAA GATTGGCCATGAACCACGAACAAGACAAAGAACAGTTAATTCAGAAGCTTAATTTTGAAAAAGATGAAGC CATTCAAACTGCACTAGATGAATTTAAGCTGGAGAGAGAACTTGTTGAGAAAGAGTTATTAGAAAAAGTT AAACATCTTGAGAATCAAATAGCTAAAAGTCCCGCCTTTGAATCAGCCAGAGAAGATTCTTCAAGCTTAG TTGCTGAACTTCAAGAGAAACTTCAGGAAGAAAAAGCTAAGTTTCTAGAACAACTTGAAGAACAGGAGAA GAGAAAGAATGAGGAAATGCAGAATGTGCGCACCTCTCTGATTGCTGAACAGCAGACCAATTTTAACACA GTCTTAACAAGAGAGAAAATGAGAAAAGAAAACATAATAAATGATCTTAGCGATAAGCTAAAAAGTACAA TGCAGCAGCAAGAGCGGGATAAAGATTTGATAGAGTCGCTGTCTGAGGACCGAGCTCGTTTGCTTGAAGA GAAGAAGAAGCTTGAAGAGGAAGTGAGTAAACTGCGCACCAGCAGCGTCCTTCCCTCAGCACCCGTGCTT GCAGCCCCAGAGCTTTATGGCGCTTGTGCACCTGAGCTCCCAGGTGAGCCAGAGCGATCAGCCATGGAGA CGACGGACGAGGGAAGAGTGGATTCAGCAATGGAGACTAGCATGATGTCTGTCCAAGAAAATATGCTATC TGAAGAGAAGCAGAGGATCATGCTCCTAGAACGGACATTGCAATTGAAAGAAGAAGAAAACAAGAGGTTA AATCAAAGATTGATGTCTCAGAGCTTGTCCTCAGTATCTTCAAGGCATTCTGAAAAAATAGCCATTAGAG ATTTTCAGGTGGGAGATTTGGTTCTCATCATCCTAGATGAGCGGCATGACAATTATGTGTTGTTTACTGT TAGTCCTACTTTGTATTTTCTGCATTCAGAGTCTCTTCCTGCCCTGGATCTCAAACCAGGTGAGGGAGCT TCAGGTGTATCTAGAAGACCCTGGGTCCTTGGAAAAGTAATGGAAAAGGAATACTGTCAAGCCAAAAAGG CACAAAACAGATTTAAAGTTCCTTTGGGGACAAAGTTTTACAGAGTGAAAGCTGTGTCATGGAATAAGAA AGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210423 representing NM_001107901 Red=Cloning site Green=Tags(s) MKLYVFLVNTGTTLTFDTELTVQTVADLKHAIQSKYKIAVQHQVLVVNGGECMTADRRVCTYSAGTDTNP IFLFNKEMILCDRAPAIPKTTFSTENDMEIKVEESLMMPAVFHTVASRTQLAVEMYEVAKKLCSFCEGLV HDEHLQHQGWAAIMANLEDCSTSYQKLLFKFESIYSDYLQSIEDIKLKLTHLGTAVSVMAKIPLLECLTR HSYRECLGRPDSLNEHEGSEKADMKRSTELVLSPDMPRTTNTSLLTSFHKSVEHVAPDVTDAEQGKELRE SCQSTVQQEDAPVDAKDSDLPFFNVSLLDWINVQDRPNDVESLVRKCFDSMSRLDPKIIQPFMLECHQTI AKLDNQNMKAIKGLEDRLYALDQMIASCSRLVNEQKELAQGFLANQMRAENLKDASVLPDLCLSHANQLM IMLQNHRKLLDIKQKCTTAKQELANNLHVRLKWCCFVMLHADQDGEKLQALLRLVIELLERVRIVEALST VPQMYCLAVVEVVRRKMFIKHYREWAGALVKDGKQLYEAEKSKRESFGKLFRKSFLRNRLFKGLDSWPSS FCTQKPRKFDCELPDISLKDLQFLQSFCPSEVQPFLRVPLLCDFEPLHQHVLALHNLVKAAQSLDEMSQT ITDLLNEQKVSTSQASPQSAASPRIESTTGITTTTSPKAPPPLTVQDTLYPAACPLEELSPDSIDAHTFD FETVSHPNTEQTVHQASIDLDSLAESPESDFMSAVNEFVIEENLSSPNPISDPQSPEMMVESLYSSVINA IDSRRMQDTSTRGNEGFGDRAALHVQLEKCRVAAQDSHISIQTIKDDLCHFRTFVQKEQCDLSNYLKCTA VEIRNIIDKVKCSLEITLKEKHQQELQSLKIEYECKLNTLEKDSEENVNKILKLKEDLVSLEEALQNKDS EFAIIKHEKDAIVCMQHEKDQKLLEMENIMHTQNSEIKELKQSREMVLEDLKKLHDEKIESLRAEFQCLE QNHLKELEDTLHIRHTEEFEKVITDHKVSLEKLKKENQQKIDQMLESHASAIQEKEQQLQELKVKVSDLS DMRCKLEVELALKEAETDEIKILLEESRTQQKETLKSLLEQETENLRTEINKLNQKIHDNSESYQVGLSE LRSLMTIEKDQCISELISRHEEESNILKAELDSVTALHHQAFEIEKKLKEQIVELQTKLNSELSALEKQK DEKITQQEEKYKALIQNLEKDKQRLAMNHEQDKEQLIQKLNFEKDEAIQTALDEFKLERELVEKELLEKV KHLENQIAKSPAFESAREDSSSLVAELQEKLQEEKAKFLEQLEEQEKRKNEEMQNVRTSLIAEQQTNFNT VLTREKMRKENIINDLSDKLKSTMQQQERDKDLIESLSEDRARLLEEKKKLEEEVSKLRTSSVLPSAPVL AAPELYGACAPELPGEPERSAMETTDEGRVDSAMETSMMSVQENMLSEEKQRIMLLERTLQLKEEENKRL NQRLMSQSLSSVSSRHSEKIAIRDFQVGDLVLIILDERHDNYVLFTVSPTLYFLHSESLPALDLKPGEGA SGVSRRPWVLGKVMEKEYCQAKKAQNRFKVPLGTKFYRVKAVSWNKKV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107901
ORF Size	4764 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107901.1, NP_001101371.1
RefSeq Size	7197 bp
RefSeq ORF	4767 bp
Locus ID	312927
MW	182.2 kDa

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