Dot1l (NM_001108733) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210536 representing NM_001108733 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGAGAAGCTGGAGCTGAGGCTCAAGTCGCCTGTGGGCGCGGAGCCCGCCGTCTACCCGTGGCCGC TGCCGGTCTACGTGAGTGTACTTGCGGAGAACCCGGGGGACAAGCACCATGATGCTGCTCATGAGATTAT CGAGACTATCCGATGGGTCTGCGAGGAAATCCCAGATCTCAAGCTGGCCATGGAGAACTATGTCCTGATC GACTACGACACCAAAAGTTTTGAAAGCATGCAGAGGCTGTGTGACAAATATAACCGGGCCATTGACAGCA TCCACCAGCTGTGGAAGGGCACTACACAGCCCATGAAGCTCAATACTCGGCCGTCCAATGGGCTCCTGCG GCACATCCTGCAGCAGGTGTACAACCACTCTGTGACCGACCCAGAGAAGCTCAACAACTACGAGCCCTTC TCCCCCGAGGTGTACGGGGAGACCTCCTTTGACCTGGTGGCCCAGATGATCGATGAGATCAAGATGACCG AGGATGACCTGTTTGTAGACCTGGGCAGTGGTGTGGGGCAGGTTGTCCTTCAGGTTGCGGCGGCCACCAA CTGCAAACATCACTACGGAGTGGAGAAAGCGGACATCCCAGCCAAGTACGCGGAGACCATGGATCGAGAG TTCAGGAAGTGGATGAAATGGTATGGAAAAAAGCATGCAGAATACACACTGGAGCGAGGTGACTTCCTCT CAGAGGAGTGGAGGGAACGGATCGCCAACACGAGTGTTATATTTGTGAATAACTTTGCCTTTGGTCCTGA GGTGGATCACCAGCTGAAGGAACGATTCGCAAACATGAAGGAAGGCGGCAGAATCGTATCCTCAAAGCCC TTTGCCCCTCTGAACTTCAGGATCAACAGCAGGAACTTGAGTGACATTGGCACCATCATGCGTGTGGTGG AGCTGTCGCCCCTGAAGGGCTCTGTGTCATGGACTGGCAAGCCTGTCTCCTACTACCTGCACACCATTGA CCGCACCATACTTGAAAACTATTTTTCTAGTCTGAAAAATCCAAAACTCAGGGAGGAGCAGGAGGCAGCT AGGCGCAGGCAGCAGCGAGAGAACAAGAGCAACGCGACCACACCCACCAAAGTCCCTGAGAGCAAGGCAG CTGCCACCGAGGCCCCTGCGGATTCTGGTGCTGAGGAAGAAAAATCAGGTGTGGCCACCGTCAAAAAGCA ATCTCCCTCCAAAGCCCGGAAGAAGAAACTGAACAAGAAAGGGAGAAAAATGGCTGGCCGGAAGCGTGGG CGACCCAAGAAAATGAGTGCTGCCAGTGCCGATCGCAAGTCCAAGAAGAGCCAAAGCACACTGGATCTCC TGCACTCTCCGCCCCCAGCCCCACCCTCAGCCTCACCCCAGGATGCATACAGGGCCCCCCACAGCCCGTT CTACCAGCTACCTCCGAGCACGCCGCTGCACTCGCCCGACCCACTGCTAGTGGCTCCCACCCCACCCGCG CTGCAGAAGCTGTTAGAGTCCTTCAAGATCCAGTACCTGCAGTTCCTGGCCTACACGAAGACCCCGCAGT ACAAGGCCAACCTGCAGCAGCTTCTGGACCAGGAGAAGGAGAAGAACACACAACTGCTGGGTACAGCCCA GCAGCTCTTCAGCCATTGCCAGGCACAAAAGGAAGAGATCCGCAGGCTGTTCCAGCAGAAGCTGGATGAG TTGGGCGTGAAGGCACTGACCTACAATGACCTGATTCAGGCCCAGAAGGAAATCTCTGCCCACAACCAGC AGCTGCGGGAGCAGTCGGAACAGCTGGAGAAGGACAACAGCGAGCTTCGGAGCCAGAGCCTGCGGCTGCT CAGGGCCCGGTGCGAAGAGCTGAGGCTTGACTGGTCCACCTTGTCCCTGGAGAACCTGCGGAAAGAGAAG CAGGCCCTGCGGAGCCAGATCTCAGAGAAGCAGCGGCACTGCCTAGAGCTGCAGATCAGCATCGTGGAGC TGGAGAAGACCCAGCGCCAGCAGGAGCTCCTGCAGTTGAAGTCCTGTGTGCCACCAGATGACGCTCTGTC CCTGCACCTGCGTGGCAAGGGTGCCCTGGGCCGCGAGCTGGAGTCTGACGCTGGGCGCTTGCGCCTTGAG CTGGACTGTGCCAAGCTCTCCCTCCCACACCTCAGCAGCATGAGCCCTGAGCTGTCCATGAATGGCCATG CCGCCAGCTATGAGCTCTGCGGTGCAGCTAGTCGGCCCTCGTCCAAGCAGAACACCCCCCAGTATCTGGC CTCCCCCTTGGACCAGGAGGTCGTGCCCTGCACCCCTAGCCACAGTGGCCGGCCTCGGCTGGAGAAGCTG TCTGGCCTGGCTTTGCCTGACTACACCCGGTTGTCACCGGCCAAGATTGTGTTGAGGCGGCACCTGAGCC AGGACCACACCGGGGCCAGCAAAGCAGCTACTAGTGAGCCACACCCTCGGCCAGAGCATGCCAAGGAGAA CAGCCTTCCCTACCAGAGCCCTGGCTTGTCGAACAGCATGAAGCTCAGCCCCCAGGATCCACCGCTGGCC TCCCCGGCAACCTCACCCCTCACTTCCGAGAGGGGTAGTGAGAAGGGTGTAAAGGAGCGTGCCTACAGCA GCCATGGGGAGACCATCACCAGCCTGCCTGTCAGCATTCCACTCAGCACAGTACAGCCCAACAAGCTGCC TGTCAGCATCCCGCTAGCCAGCGTGGTGCTGCCCAGCCGTGCCGAGAGGGCGAGGAGCACTCCCAGCCCT GTGCCCCAGCCTCGAGACTCCTCATCCACACTTGAGAAGCAGATTGGTGCTTCCACCCATGGTGCAGGGG GTGCTGCGGCAGGAAGCAGGAGCCTCACTGTGGCGCCCACAGGTTTCTATGCTGGTTCGGTGGCCATCAG TGGGGCTCTGGCCAGCAGCCCAGCACCTCTGGCCTCTGGAATGGAATCTGCTGTTTTTGAGGAGTCCTCT GGCCCCAGCAGCCTCTTTGGCACCATGGGGTCTCGCAGCACACCGCCACAACATCCACCTCTCCTGCCGC AGTCTCGCAACTCGGGCCCTGCCTCTCCTGCCCACCAGCTCGCAGCCAGTCCCCGCCTGAGTGTGACCAC CCAGGGCTCACTGCCAGACACCGGCAAAGGGGAGCTGCCTGCTGATCCTGCCTTCTCAGACCCAGAGAGT GAAGCTAAGAGGAGGATTGTGTTCAGCATTTCAGCTGGTGCCAGCTCCAGGCAGTCGCCTTCCACCAGGC ACAGCCCCTTGACCTCTGGCACCCGTGGGGATTGTGTTCAGAGCCATGGGCAGGACAGTCGTAAGCGTAG CAGAAGGAAGCGTGCATCAGCTGGAACGCCTAGCCTCACCACGGGTGTGTCCCCCAAGCGCCGAGCTCTG CCAACCGTCGCTGGCCTCTTTACACAGTCTTCGGGGTCGCCTCTCAATCTCACCTCCATGGTCAGCAACA TCAACCAGCCCCTGGAGATCACAGCCATCTCATCCCCGGAGAGCTCACTGAAGAGCTCCCCCACACCCTA CCAGGACCATGACCAGCCTCCAGTGCTCAGGAAGGAGCGGCCCCTGGGCCCAACTAATGGGGCCCACTAC TCTCCGCTGACGTCAGATGAGGAGCCAGGCTCGGAGGACGAGCCCAGCAGTGCCCGAATTGAGAGAAAAA TTGCAACAATCTCTTTAGAAAGCAAATCTCCCCCGAAGACACTGGAAAATGGTGGTGGCTTAGTGGGAAG GAAGCCGGTCCCCTCAGGCGAGCCTGTCAACAGCAGCAAATGGAAGTCCACCTTCTCACCCATCTCCGAC CTCGGCTTGGCCAAGGCCATGGACAGTCCGCTACAGGCCAGCTCTGCACTGAGCCACAGCCCCCTCTTCT CTTTCCGGCCGTCCTTGGAGGAGCCTGCTGCTGAGGCCAAGCCCTCCACCCACCCAAGGAAAAGTTTTGC GGGCTCTCTGGCTGCAGCTGAGGGGCCAAGCCCTGGCACCAACCCTCCCAACAGCCTGGCCTTCAGTGGG GGCCTTGCTGCAGACCTCGGTTTACATAGCTTCAATGACGGTGCTTCCCTCTCCCACAAGGGCCCGGAGG TGGCGGGCCTGAGCGCCTCCCTGAGCTTCCCATCCCAGAGGGGCAAGGACAGTACCACAGAGGCCAACCC CTTCCTCAGCAGGCGGCAGCCGGAGGGCCTGGGTGGCCTGAAGGGTGAGGGCAATGCAAGCAAGGAGTCA GGAGAGAGCCTGCCCCTGTGTGGGCCCTCAGACAAGGTCTCACTGCCACACGGCAGCAGGGCCAGCAAAG GCCGTGACCGCGAGCTGGACTTCAAGGGCGGCCACAACCTCTTCATCTCTGCTGCAGCCGTGCCTCCAGG TGGCCTCCTTGGTGGCCCTGGTCTTGTAACTGTGGCTTCCTCTGCGGGCAGTGCGACACCCTCTGCCCAG GCTCCCCGGCCTTTCCTGAGCACCTTTGCCCCTGGGCCCCAGTTTACTCTGGGCCCCATGTCCCTGCAGG CCAACCTGGGCTCTGTGGCCGGCTCCTCTGTGCTGCAGTCCTTGTTCAGCACTGTGCCAGCTGCTGCAGG CCTGGTGCATGTGTCATCCACTGCGACCCGACTGACCAACTCACACACCATGGGCAGCTTCTCCTCTGGG GTGGCCGGCGGAACCGTTGGAGGTAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210536 representing NM_001108733 Red=Cloning site Green=Tags(s) MGEKLELRLKSPVGAEPAVYPWPLPVYVSVLAENPGDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLI DYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSNGLLRHILQQVYNHSVTDPEKLNNYEPF SPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDRE FRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKP FAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAA RRRQQRENKSNATTPTKVPESKAAATEAPADSGAEEEKSGVATVKKQSPSKARKKKLNKKGRKMAGRKRG RPKKMSAASADRKSKKSQSTLDLLHSPPPAPPSASPQDAYRAPHSPFYQLPPSTPLHSPDPLLVAPTPPA LQKLLESFKIQYLQFLAYTKTPQYKANLQQLLDQEKEKNTQLLGTAQQLFSHCQAQKEEIRRLFQQKLDE LGVKALTYNDLIQAQKEISAHNQQLREQSEQLEKDNSELRSQSLRLLRARCEELRLDWSTLSLENLRKEK QALRSQISEKQRHCLELQISIVELEKTQRQQELLQLKSCVPPDDALSLHLRGKGALGRELESDAGRLRLE LDCAKLSLPHLSSMSPELSMNGHAASYELCGAASRPSSKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKL SGLALPDYTRLSPAKIVLRRHLSQDHTGASKAATSEPHPRPEHAKENSLPYQSPGLSNSMKLSPQDPPLA SPATSPLTSERGSEKGVKERAYSSHGETITSLPVSIPLSTVQPNKLPVSIPLASVVLPSRAERARSTPSP VPQPRDSSSTLEKQIGASTHGAGGAAAGSRSLTVAPTGFYAGSVAISGALASSPAPLASGMESAVFEESS GPSSLFGTMGSRSTPPQHPPLLPQSRNSGPASPAHQLAASPRLSVTTQGSLPDTGKGELPADPAFSDPES EAKRRIVFSISAGASSRQSPSTRHSPLTSGTRGDCVQSHGQDSRKRSRRKRASAGTPSLTTGVSPKRRAL PTVAGLFTQSSGSPLNLTSMVSNINQPLEITAISSPESSLKSSPTPYQDHDQPPVLRKERPLGPTNGAHY SPLTSDEEPGSEDEPSSARIERKIATISLESKSPPKTLENGGGLVGRKPVPSGEPVNSSKWKSTFSPISD LGLAKAMDSPLQASSALSHSPLFSFRPSLEEPAAEAKPSTHPRKSFAGSLAAAEGPSPGTNPPNSLAFSG GLAADLGLHSFNDGASLSHKGPEVAGLSASLSFPSQRGKDSTTEANPFLSRRQPEGLGGLKGEGNASKES GESLPLCGPSDKVSLPHGSRASKGRDRELDFKGGHNLFISAAAVPPGGLLGGPGLVTVASSAGSATPSAQ APRPFLSTFAPGPQFTLGPMSLQANLGSVAGSSVLQSLFSTVPAAAGLVHVSSTATRLTNSHTMGSFSSG VAGGTVGGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108733
ORF Size	4647 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108733.1, NP_001102203.1
RefSeq Size	6015 bp
RefSeq ORF	4650 bp
Locus ID	362831
MW	166.8 kDa

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