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Hck (NM_013185) Rat Tagged ORF Clone
CAT＃: RR210580
- TrueORF®
Hck (Myc-DDK-tagged ORF) - Rat hemopoietic cell kinase (Hck), (10 ug)
"NM_013185" in other vectors (3)
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CNY 6,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Hctk; p56Hck; p59Hck |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210580 representing NM_013185
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGGGGGGTCGTTCGAGCTGCGAGGATCCGGGCTGCCCGCGAGGCGAAGGGCGGGTGCCCAGGATGGGAT GTGTGAAGTCCAGGTTCCTCCGAGAAGGAAGCAAGGCCTCAAAAATAGAGCCAAATGCCAACCAGAAAGG CCCTGTGTATGTGCCGGATCCCACGTCCCCTAAGAAGCTGGGACCGAACAGCATCAACAGCCTGCCCCCG GGGGTCGTGGAGGGCTCTGAGGACACCATTGTGGTCGCACTGTACGACTATGAGGCCATTCACCGTGAAG ACCTCAGCTTCCAGAAGGGAGACCAGATGGTGGTTCTGGAGGAGTCTGGGGAGTGGTGGAAGGCCCGTTC CCTGGCTACCAAGAAAGAAGGCTATATCCCAAGCAATTATGTAGCTCGAGTTAACTCTTTGGAGACTGAG GAGTGGTTCTTCAAGGGTATCAGCCGGAAGGATGCAGAGCGCCACCTGCTGGCTCCCGGGAACATGCTGG GCTCCTTCATGATCCGGGACAGTGAGACCACCAAAGGGAGCTACTCACTTTCTGTTCGAGACTTTGACCC CCAGCACGGAGACACGGTGAAGCATTATAAAATCCGGACACTGGACAGTGGAGGCTTCTACATCTCTCCG AGGAGCACCTTCAGCAGCCTGCAGGAACTTGTCGTCCACTACAAGAAGGGGAAGGATGGGCTCTGCCAGA AGCTGTCAGTGCCCTGTGTGTCTCCCAAACCCCAGAAGCCATGGGAGAAAGATGCCTGGGAGATTCCTCG AGAATCCCTCCAGATGGAGAAGAAACTGGGAGCCGGGCAGTTTGGAGAAGTGTGGATGGCCACCTACAAC AAGCACACCAAAGTGGCGGTGAAGACAATGAAGCCAGGGAGCATGTCTGTGGAGGCCTTCCTGGCAGAGG CCAACCTGATGAAGACGTTACAGCATGATAAACTGGTGAAGCTGCACGCCGTGGTCTCTCAGGAGCCCAT CTTTATTGTCACCGAGTTCATGGCCAAAGGAAGCCTGCTGGACTTTCTCAAGAGTGAAGAAGGCAGCAAG CAGCCACTGCCAAAACTCATTGACTTCTCAGCCCAGATTTCAGAGGGCATGGCTTTCATTGAGCAGAGGA ACTACATCCACCGAGACCTCCGGGCTGCCAACATCTTGGTTTCTGCATCACTGGTGTGTAAGATCGCTGA CTTTGGACTGGCACGGATCATCGAGGACAATGAGTACACAGCTCGGGAAGGAGCCAAGTTCCCCATCAAG TGGACAGCTCCTGAAGCCATCAACTTTGGCTCCTTCACCATCAAGTCAGATGTCTGGTCCTTTGGTATCC TGCTGATGGAAATCGTCACCTACGGCCGGATCCCTTACCCAGGTATGTCAAACCCAGAGGTGATTCGAGC ACTAGAGCATGGGTACCGTATGCCTCGACCAGATAACTGCCCAGAGGAGCTCTACAGTATCATGATCCGC TGCTGGAAGAACCGTCCAGAGGAACGGCCCACTTTCGAATACATCCAGAGCGTGCTGGATGACTTCTACA CGGCCACTGAGAGCCAGTATCAGCAGCAACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210580 representing NM_013185
Red=Cloning site Green=Tags(s) LGGRSSCEDPGCPRGEGRVPRMGCVKSRFLREGSKASKIEPNANQKGPVYVPDPTSPKKLGPNSINSLPP GVVEGSEDTIVVALYDYEAIHREDLSFQKGDQMVVLEESGEWWKARSLATKKEGYIPSNYVARVNSLETE EWFFKGISRKDAERHLLAPGNMLGSFMIRDSETTKGSYSLSVRDFDPQHGDTVKHYKIRTLDSGGFYISP RSTFSSLQELVVHYKKGKDGLCQKLSVPCVSPKPQKPWEKDAWEIPRESLQMEKKLGAGQFGEVWMATYN KHTKVAVKTMKPGSMSVEAFLAEANLMKTLQHDKLVKLHAVVSQEPIFIVTEFMAKGSLLDFLKSEEGSK QPLPKLIDFSAQISEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARIIEDNEYTAREGAKFPIK WTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALEHGYRMPRPDNCPEELYSIMIR CWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013185 |
| ORF Size | 1572 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013185.3, NP_037317.2 |
| RefSeq Size | 2081 bp |
| RefSeq ORF | 1575 bp |
| Locus ID | 25734 |
| UniProt ID | P50545 |
| MW | 59.1 kDa |
| Gene Summary | The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types.The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms in human and mouse. Based on strong sequence conservation amongst these species, this may also be true for rat. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210580 | Hck (untagged ORF) - Rat hemopoietic cell kinase (Hck), (10 ug) |
CNY 6,750.00 |
|
| RR210580L3 | Lenti ORF clone of Hck (Myc-DDK-tagged ORF) - Rat hemopoietic cell kinase (Hck), (10 ug) |
CNY 8,650.00 |
|
| RR210580L4 | Lenti ORF clone of Hck (mGFP-tagged ORF) - Rat hemopoietic cell kinase (Hck), (10 ug) |
CNY 9,500.00 |
