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Tert (NM_053423) Rat Tagged ORF Clone
CAT＃: RR210889
- TrueORF®
Tert (Myc-DDK-tagged ORF) - Rat telomerase reverse transcriptase (Tert), (10 ug)
"NM_053423" in other vectors (3)
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CNY 18,150.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210889 representing NM_053423
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGCGCTCCTCGTTGCCCCGCCGTGCGCTCTCTACTGCGCAGCCGATATCGGGAGGTGTGGCCGC TGGCGACCTTTGTGCGGCGCCTGGGGCTTGAGGGCAGTCGGCTTGTGCAACCCGGGGACCCGAAGGTCTT CCGCACGTTGGTTGCCCAGTGCCTAGTGTGCGTGCCCTGGGGCTCACAGCCGCCACCTGCTGACCTTTCC TTCCACCAGGTGTCATCCCTGAAAGAGCTGGTGTCCAGGGTTGTGCAGAAACTTTGCGAGCGCGGTGAGA GGAATGTGCTGGCTTTTGGCTTTGCACTGCTTAACGGGGCCAGAGGTGGGCCTCCCATGGCCTTCACGAC CAGCGTGCATAGCTACTTGCCCAACTCGGTTACTGAGTCCCTGTGTGTCAGTGGTGCATGGATGCTACTG TTGAGCCGAGTGGGCGACGACCTGCTGGTCTACCTGCTGTCGCACTGTGCGCTCTACCTGCTGGTGCCCC CCAGCTGTGCCTACCAGGTGTGCGGGTCACCCCTGTACCAAATTTGTGCCACCACGGATACCTGGTCCTC TGTGCCCGCTGGTTACAGGCCCACTCGACCCGTGGGCGGGAATTTCACTAACCTTGGGTCCGCACACCAG ATCAAAAACAGTGGTCACCAGGAAGCACCAAAACCCCAGGCCTTGCCATCACGAGGTACGAAGAGGCTTC TGAGTCTCACCAGTACAAACGTGCCTTCAGCTAAGAAGGCCAGGTTTGAACCTGCCCTGAGAGTGGATAA GGGACCCCACAGGCAGGTGGTACCAACCCCATCAGGCAAAACATGGGCGCCAAGTCCTGCTGCGTCCCCC AAGGTGCCTCCTGCAGCGAAAAACTTGTCTTTGAAAGGAAAGGCATCTGACCCGAGTCTCTCTGGGTCGG TGTGCTGTAAACACAAGCCCAGCTCCTCGTCCCTGCTGTCATCACCACCCCAAGATGCTGAAAAGCTCAG GCCATTCACTGAGACCAGACATTTCCTTTACTCCAGGGGAGGTGGCCAAGAGGAGCTAAATCCCTCATTC CTACTCAACAGCCTCCCGCCTAGCTTGACCGGGGCCAGGAGACTGGTGGAGATCATCTTTCTGGGCTCAA GGCCTAGGACATCAGGACCATTCTGCAGGACCCGCCGCCTGCCCCGTCGATACTGGCAGATGCGACCCCT ATTCCAGCAGCTGCTCATGAACCACGCAAAGTGCCAATATGTCAGATTCCTCCGGTCGCACTGCAGATTT CGAACAGCAAACCAGCGGGTGCCGGATGCCATGGACACCAGCCCATCCCACCTCACGAGTTTGCTCCGGT TACACAGCAGCCCCTGGCAGGTATACGGCTTTCTTCGGGCCTGCCTCCGCGAGCTGGTGCCTGCCGGTCT CTGGGGCACCAGGCACAATGAGCGCCGCTTCTTAAAGAACGTGAAGAAGTTCATCTCGTTGGGGAAGTAC GCCAAGCTATCCCTGCAGGAACTGATGTGGAGGGTGAAAGTGGAGGACTGCCACTGGCTCCGCAGCAGCC CAGAGAAGGACACTGTCCCTGCCGCAGAGCACCGTCTGAGGGAGAGGATCCTTGCCATGTTCCTGTTCTG GCTAATGGACACATATGTGGTACAGCTGCTGAGGTCATTCTTCTACATCACAGAGACCACGTTCCAGAAG AACCGCCTTTTCTTCTACCGTAAGAGTGTGTGGAGCAAGCTGCAGAGCATTGGAATCAGGCAACAGCTTG AGAGAGTTCAGCTACGGGAACTGTCACAAGAGGAGGTCAAGCATCACCAGGACACTTGGCTGGCCATGCC TATCTGCAGATTGCGCTTCATCCCCAAGCTCAATGGTCTCCGGCCCATTGTGAACATGAGTTATGGCATG GACACCAGAGCTTTTGGCAAAAAGAAGCAGACCCAGTGTTTCACTCAGAGTCTCAAGACTTTGTTCAGCG TGCTCAACTACGAGCGGACCAAACATCCTAACCTTATGGGTGCTTCAGTACTGGGTACGAGTGACAGCTA CAGGATCTGGCGGACCTTCGTGCTGCGTGTGCGTGCTCTGGACCAGACACCCAGGATGTACTTTGTTAAG GCAGATGTGACAGGGGCCTATGATGCCATCCCCCAGGACAAGCTCGTGGAAATTGTCGCCAATATAATCA GGCGCTCAGAGAGCATGTACTGTATCCGCCAGTATGCAGTGGTTCAGAAAGATAGCCAAGGCCAAGTCCA CAAGTCCTTCAGGAGACAGGTCTCCACCCTCTCTGACCTCCAGCCATACATGGGCCAGTTCACCAAGCAT CTGCAGGACTCAGATGCCAGTGCACTGAGGAACTCTGTTGTCATCGAGCAGAGCATCTCCATGAATGAGA CTGGCAGTAGCCTGCTCCACTTCTTCCTGCGCTTTGTCCGTCACAGTGTCGTGAAGATCGATGGCAGGTT CTATGTGCAATGCCAGGGCATCCCCCAGGGCTCCAGCCTGTCCACCCTGCTCTGCAGTCTGTGTTTCGGA GACATGGAGAACAAGCTGTTTGCCGAGGTGCAGCAGGACGGCTTGCTTTTACGTTTTGTCGATGACTTTC TGTTGGTGACACCTCACCTGGCCCATGCAAAAGCCTTTCTCAGCACCCTGGTCCATGGCGTGCCCGAGTA TGGCTGCATGATAAACTTGCAGAAGACAGTGGTGAACTTCCCTGTGGAGACCGGCGCCCTGGGAGGTGCA GCCCCGCACCAGCTGCCTGCTCACTGCCTGTTTCCCTGGTGTGGCTTACTGCTGGACACTCGGACTTTGG AAGTATTCTGTGACTACTCAGGTTACGGACGGACCTCAATTAAGATGAGCCTCACCTTCCAGGGTGTCTC CAGGGCCGGGAAGACCATGCGGTACAAGCTCTTGTCAGTCTTGCGGTTGAAGTGTCATGGTCTGTTTCTA GACTTGCAGGTGAACAGCCTGCAGACAGTCTGCATCAATATATACAAGATCTTCCTGCTTCAGGCCTACA GGTTCCATGCATGTGTGATTCGGCTTCCCTTTGGCCAGCATGTTAGGAAGAACCATGCATTCTTTCTGGG CATCATCTCCAACCTAGCATCCTGCTGCTACGCCATCCTGAAGGTCAAGAATCCAGGAGTGTCACTAAGG GCCAAGGGTGCCCCTGGCTCCTTTCCGCCCGAGGCCACACGTTGGCTCTGCTACCAAGCCTTCCTGCTCA AGCTGGCTGCTCATTCTGTCACCTACAAGTGTCTCCTGGGACCTCTTAGGACAGCCCAAAAACAGCTGTG CCGGAAGCTCCCAGAGGCAACAATGACCCTCCTTAAGACTGCAGCTGACCCAGCCCTAAGCACAGATTTT CAGACCATTTTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210889 representing NM_053423
Red=Cloning site Green=Tags(s) MPRAPRCPAVRSLLRSRYREVWPLATFVRRLGLEGSRLVQPGDPKVFRTLVAQCLVCVPWGSQPPPADLS FHQVSSLKELVSRVVQKLCERGERNVLAFGFALLNGARGGPPMAFTTSVHSYLPNSVTESLCVSGAWMLL LSRVGDDLLVYLLSHCALYLLVPPSCAYQVCGSPLYQICATTDTWSSVPAGYRPTRPVGGNFTNLGSAHQ IKNSGHQEAPKPQALPSRGTKRLLSLTSTNVPSAKKARFEPALRVDKGPHRQVVPTPSGKTWAPSPAASP KVPPAAKNLSLKGKASDPSLSGSVCCKHKPSSSSLLSSPPQDAEKLRPFTETRHFLYSRGGGQEELNPSF LLNSLPPSLTGARRLVEIIFLGSRPRTSGPFCRTRRLPRRYWQMRPLFQQLLMNHAKCQYVRFLRSHCRF RTANQRVPDAMDTSPSHLTSLLRLHSSPWQVYGFLRACLRELVPAGLWGTRHNERRFLKNVKKFISLGKY AKLSLQELMWRVKVEDCHWLRSSPEKDTVPAAEHRLRERILAMFLFWLMDTYVVQLLRSFFYITETTFQK NRLFFYRKSVWSKLQSIGIRQQLERVQLRELSQEEVKHHQDTWLAMPICRLRFIPKLNGLRPIVNMSYGM DTRAFGKKKQTQCFTQSLKTLFSVLNYERTKHPNLMGASVLGTSDSYRIWRTFVLRVRALDQTPRMYFVK ADVTGAYDAIPQDKLVEIVANIIRRSESMYCIRQYAVVQKDSQGQVHKSFRRQVSTLSDLQPYMGQFTKH LQDSDASALRNSVVIEQSISMNETGSSLLHFFLRFVRHSVVKIDGRFYVQCQGIPQGSSLSTLLCSLCFG DMENKLFAEVQQDGLLLRFVDDFLLVTPHLAHAKAFLSTLVHGVPEYGCMINLQKTVVNFPVETGALGGA APHQLPAHCLFPWCGLLLDTRTLEVFCDYSGYGRTSIKMSLTFQGVSRAGKTMRYKLLSVLRLKCHGLFL DLQVNSLQTVCINIYKIFLLQAYRFHACVIRLPFGQHVRKNHAFFLGIISNLASCCYAILKVKNPGVSLR AKGAPGSFPPEATRWLCYQAFLLKLAAHSVTYKCLLGPLRTAQKQLCRKLPEATMTLLKTAADPALSTDF QTILD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053423 |
| ORF Size | 3375 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053423.1, NP_445875.1 |
| RefSeq Size | 3378 bp |
| RefSeq ORF | 3378 bp |
| Locus ID | 301965 |
| UniProt ID | Q673L6 |
| MW | 126.9 kDa |
| Gene Summary | Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210889 | Tert (untagged ORF) - Rat telomerase reverse transcriptase (Tert), (10 ug) |
CNY 18,150.00 |
|
| RR210889L3 | Lenti ORF clone of Tert (Myc-DDK-tagged ORF) - Rat telomerase reverse transcriptase (Tert), (10 ug) |
CNY 19,570.00 |
|
| RR210889L4 | Lenti ORF clone of Tert (mGFP-tagged ORF) - Rat telomerase reverse transcriptase (Tert), (10 ug) |
CNY 21,570.00 |
