Abcb1b (NM_012623) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Abcb1; Mdr1; mdr1b; Pgy1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR211334 representing NM_012623 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTTGAAGAGGGCCTTAACGGAACAGCAGACAAGAACTTCTCAAAGATGGGCAAAAAGAGTAAAA AGGAGAAGGAGAAGAAACCTGCTGTTGGCATATTCGGGATGTTTCGCTATGCAGATTGGCTTGACAAGCT GTGCATGGCTCTGGGAACTCTCGCTGCTATCATCCACGGAACCCTGCTTCCCCTCCTGATGCTGGTGTTC GGATACATGACAGATAGTTTTACCCAAGCAGAGACCCGCATTCTGCCGAGCGTTACTAATCAAAGTGAAA TCAACAGTACACAGACCGTCAGCGACAGCAGTCTGGAGGAGGACATGGCCATGTACGCCTACTATTACAC GGGCATTGGTGCCGGTGTGCTCATCGTTGCCTACATCCAGGTTTCACTTTGGTGCCTGGCAGCTGGGAGA CAAATACACAAGATTAGGCAGAAGTTTTTCCATGCCATCATGAATCAGGAGATAGGCTGGTTTGACGTGA ATGACGCTGGGGAGCTCAACACCCGGCTCACAGATGACGTCTCCAAAATTAATGACGGAATTGGTGACAA ACTTGGAATGTTCTTTCAGTCCATAACGACATTTTCAGCCGGTTTTATAATAGGATTTATAAGTGGTTGG AAGCTAACCCTTGTAATTTTGGCCGTCAGCCCTCTTATTGGGTTGTCATCTGCCATGTGGGCAAAGGTAC TGACTTCATTTACTAATAAGGAACTCCAGGCTTATGCGAAAGCTGGAGCAGTTGCCGAAGAAGTCTTAGC AGCCATCAGAACTGTGATTGCATTTGGAGGACAAAAGAAGGAACTTGAAAGGTACAATAAAAATTTAGAA GAAGCTAAAAGAGTTGGCATAAAGAAAGCCATCACGGCCAACATTTCCATAGGTATTGCCTACCTGTTGG TCTATGCGTCTTATGCACTGGCATTCTGGTATGGGACCTCCTTGGTCCTCTCAAATGAATATTCTATTGG ACAAGTGCTTACCGTCTTCTTCTCTATTTTATTGGGGACTTTCAGTATTGGACATTTAGCCCCAAACATA GAAGCCTTTGCAAATGCAAGAGGGGCAGCCTATGAAATCTTCAAGATAATTGATAATGAGCCAAGCATTG ACAGCTTCTCAACCAAGGGACACAAACCAGACAGTATAATGGGAAATTTGGAATTTAAAAATGTTTACTT CAACTACCCATCACGAAGTGAAGTTAAGATCTTGAAGGGCCTCAACCTGAAGGTGAAGAGCGGGCAGACG GTAGCCCTGGTTGGCAACAGTGGCTGTGGGAAAAGCACAACTGTCCAGCTGCTGCAGAGGCTCTACGACC CCATAGAGGGCGAGGTCAGTATCGACGGACAGGACATCAGGACCATCAATGTGAGGTATCTGCGGGAAAT CATTGGGGTGGTGAGTCAGGAACCCGTGCTGTTTGCCACCACGATTGCCGAAAACATTCGCTATGGCCGA GAAAACGTCACCATGGATGAGATAGAGAAAGCTGTCAAGGAAGCCAATGCCTATGACTTCATCATGAAAC TGCCCCACAAATTTAACACCCTGGTTGGTGAGAGAGGGGCGCAGCTGAGTGGGGGACAGAAACAGAGGAT CGCCATTGCCCGGGCCCTGGTCCGCAACCCCAAGATCCTTTTGTTGGATGAGGCCACGTCAGCCTTGGAC ACAGAAAGCGAAGCCGTGGTTCAGGCCGCTCTGGATAAGGCTAGAGAAGGCCGGACCACCATTGTGATAG CTCACCGCTTGTCTACAGTGCGCAATGCTGACGTCATTGCTGGTTTTGATGGTGGTGTCATTGTGGAGCA AGGAAATCATGAAGAGCTCATGAAAGAGAAGGGCATTTACTTCAAACTTGTCATGACACAGACTAGAGGA AATGAAATTGAACCAGGAAATAATGCTTATGAATCCCAAAGTGACACTGGTGCCTCTGAGTTGACTTCAG AAAAATCAAAATCTCCTTTAATAAGGAGATCAATTCGCAGAAGTATCCACAGAAGACAAGACCAGGAGAG AAGACTTAGTTCGAAAGAGGATGTGGATGAAGATGTGCCTATGGTTTCCTTTTGGCAGATCCTAAAGCTA AATATTAGTGAATGGCCCTATTTAGTTGTGGGTGTACTTTGTGCTGTTATAAATGGGTGCATACAACCAG TGTTTGCCATAGTGTTTTCAAAGATTGTAGGGGTTTTTTCAAGAGACGACGACCATGAAACCAAACAACG GAATTGTAACTTGTTTTCCCTTCTCTTTCTGGTCATGGGAATGATTTCTTTTGTTACGTACTTCTTTCAA GGCTTCGCATTTGGCAAAGCTGGAGAGATCCTCACCAAGCGACTCCGATACATGGTCTTCAAATCCATGC TGCGACAGGATATAAGCTGGTTTGATGACCATAAAAACACCACTGGCTCGCTGACTACCAGGCTCGCTAG TGACGCTTCTAATGTTAAAGGGGCTATGGGCTCCAGGCTTGCTGTAGTTACCCAGAATGTAGCAAACCTT GGCACAGGAATTATCTTATCCTTAGTCTATGGCTGGCAGCTTACACTTTTACTTGTAGTAATTATACCAC TCATTGTCTTGGGTGGAATTATTGAAATGAAACTGTTGTCTGGTCAAGCCTTGAAGGACAAGAAAGAGCT AGAGATCTCTGGGAAGATCGCTACAGAAGCAATTGAAAACTTCCGCACTGTTGTCTCTTTGACTCGGGAG CAGAAGTTTGAAACTATGTATGCCCAGAGCTTGCAGATACCATACAGAAATGCTTTGAAGAAAGCACACG TCTTTGGGATCACCTTCGCCTTCACCCAGGCCATGATTTATTTCTCCTATGCTGCTTGTTTCCGGTTCGG TGCCTACTTGGTGGCACGAGAACTCATGACATTTGAAAATGTTATGTTGGTATTTTCTGCTGTTGTCTTT GGTGCCATGGCAGCAGGGAATACCAGTTCATTCGCTCCTGACTACGCGAAGGCCAAAGTCTCAGCATCCC ACATCATCAGGATCATTGAGAAAATCCCCGAGATTGACAGCTACAGCACGGAGGGCTTGAAGCCTAATTG GTTAGAAGGAAATGTGAAATTTAATGGAGTCATGTTCAACTATCCCACCCGACCCAACATCCCAGTGCTT CAGGGACTGAGCTTCGAGGTGAAGAAGGGGCAGACGCTCGCCCTGGTGGGCAGCAGTGGCTGCGGGAAGA GCACGGTGGTCCAGCTGCTCGAGCGCTTCTACAACCCCATGGCTGGAACAGTGTTTCTAGATGGCAAAGA AATAAAACAACTCAACGTCCAGTGGCTCCGCGCCCACCTGGGCATTGTGTCCCAGGAGCCCATCCTGTTT GACTGCAGCATCACCGAGAACATCGCCTACGGAGACAACAGCCGTGTCGTGTCTCATGAGGAGATCGTGA GGGCCGCCAGGGAGGCCAACATCCACCAGTTCATCGACTCACTGCCTGAGAAATACAACACCAGAGTGGG AGACAAAGGGACTCAGCTGTCGGGCGGGCAGAAGCAGCGCATCGCCATCGCGCGCGCCCTCGTCAGACAG CCTCACATCTTACTTCTGGATGAAGCGACATCAGCTCTGGATACGGAGAGTGAAAAGGTCGTCCAGGAAG CGCTGGACAAAGCCAGGGAAGGCCGCACCTGCATTGTGATCGCGCACCGCCTGTCCACCATCCAGAACGC AGACTTGATCGTGGTGATTCAGAACGGCCAGGTCAAGGAGCACGGCACCCACCAGCAGCTGCTGGCCCAG AAAGGCATCTATTTCTCGATGGTTCAGGCTGGAGCAAAGCGCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR211334 representing NM_012623 Red=Cloning site Green=Tags(s) MEFEEGLNGTADKNFSKMGKKSKKEKEKKPAVGIFGMFRYADWLDKLCMALGTLAAIIHGTLLPLLMLVF GYMTDSFTQAETRILPSVTNQSEINSTQTVSDSSLEEDMAMYAYYYTGIGAGVLIVAYIQVSLWCLAAGR QIHKIRQKFFHAIMNQEIGWFDVNDAGELNTRLTDDVSKINDGIGDKLGMFFQSITTFSAGFIIGFISGW KLTLVILAVSPLIGLSSAMWAKVLTSFTNKELQAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLE EAKRVGIKKAITANISIGIAYLLVYASYALAFWYGTSLVLSNEYSIGQVLTVFFSILLGTFSIGHLAPNI EAFANARGAAYEIFKIIDNEPSIDSFSTKGHKPDSIMGNLEFKNVYFNYPSRSEVKILKGLNLKVKSGQT VALVGNSGCGKSTTVQLLQRLYDPIEGEVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGR ENVTMDEIEKAVKEANAYDFIMKLPHKFNTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALD TESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHEELMKEKGIYFKLVMTQTRG NEIEPGNNAYESQSDTGASELTSEKSKSPLIRRSIRRSIHRRQDQERRLSSKEDVDEDVPMVSFWQILKL NISEWPYLVVGVLCAVINGCIQPVFAIVFSKIVGVFSRDDDHETKQRNCNLFSLLFLVMGMISFVTYFFQ GFAFGKAGEILTKRLRYMVFKSMLRQDISWFDDHKNTTGSLTTRLASDASNVKGAMGSRLAVVTQNVANL GTGIILSLVYGWQLTLLLVVIIPLIVLGGIIEMKLLSGQALKDKKELEISGKIATEAIENFRTVVSLTRE QKFETMYAQSLQIPYRNALKKAHVFGITFAFTQAMIYFSYAACFRFGAYLVARELMTFENVMLVFSAVVF GAMAAGNTSSFAPDYAKAKVSASHIIRIIEKIPEIDSYSTEGLKPNWLEGNVKFNGVMFNYPTRPNIPVL QGLSFEVKKGQTLALVGSSGCGKSTVVQLLERFYNPMAGTVFLDGKEIKQLNVQWLRAHLGIVSQEPILF DCSITENIAYGDNSRVVSHEEIVRAAREANIHQFIDSLPEKYNTRVGDKGTQLSGGQKQRIAIARALVRQ PHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGQVKEHGTHQQLLAQ KGIYFSMVQAGAKRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012623
ORF Size	3825 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012623.2, NP_036755.2
RefSeq Size	3888 bp
RefSeq ORF	3828 bp
Locus ID	24646
MW	141.2 kDa
Gene Summary	member of the ATP-binding cassette (ABC) protein superfamily; may play a role in drug disposition [RGD, Feb 2006]

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