Scn10a (NM_017247) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Na(V)1.8; Nav1.8; PN3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR211430 representing NM_017247 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTCCCCTTTGCGTCCGTGGGAACTACCAATTTCAGACGGTTCACTCCAGAGTCACTGGCAGAGA TCGAGAAGCAGATTGCTGCTCACCGCGCAGCCAAGAAGGCCAGAACCAAGCACAGAGGACAGGAGGACAA GGGCGAGAAGCCCAGGCCTCAGCTGGACTTGAAAGACTGTAACCAGCTGCCCAAGTTCTATGGTGAGCTC CCAGCAGAACTGGTCGGGGAGCCCCTGGAGGACCTAGACCCTTTCTACAGCACACACCGGACATTCATGG TGTTGAATAAAAGCAGGACCATTTCCAGATTCAGTGCCACTTGGGCCCTGTGGCTCTTCAGTCCCTTCAA CCTGATCAGAAGAACAGCCATCAAAGTGTCTGTCCATTCCTGGTTCTCCATATTCATCACCATCACTATT TTGGTCAACTGCGTGTGCATGACCCGAACTGATCTTCCAGAGAAAGTCGAGTACGTCTTCACTGTCATTT ACACCTTCGAGGCTCTGATTAAGATACTGGCAAGAGGGTTTTGTCTAAATGAGTTCACTTATCTTCGAGA TCCGTGGAACTGGCTGGACTTCAGTGTCATTACCTTGGCGTATGTGGGTGCAGCGATAGACCTCCGAGGA ATCTCAGGCCTGCGGACATTCCGAGTTCTCAGAGCCCTGAAAACTGTTTCTGTGATCCCAGGACTGAAGG TCATCGTGGGAGCCCTGATCCACTCAGTGAGGAAGCTGGCCGACGTGACTATCCTCACAGTCTTCTGCCT GAGCGTCTTCGCCTTGGTGGGCCTGCAGCTCTTTAAGGGGAACCTTAAGAACAAATGCATCAGGAACGGA ACAGATCCCCACAAGGCTGACAACCTCTCATCTGAAATGGCAGAATACATCTTCATCAAGCCTGGTACTA CGGATCCCTTACTGTGCGGCAATGGGTCTGATGCTGGTCACTGCCCTGGAGGCTATGTCTGCCTGAAAAC TCCTGACAACCCGGATTTTAACTACACCAGCTTTGATTCCTTTGCGTGGGCATTCCTCTCACTGTTCCGC CTCATGACGCAGGACTCCTGGGAGCGCCTGTACCAGCAGACACTCCGGGCTTCTGGGAAAATGTACATGG TCTTTTTCGTGCTGGTTATTTTCCTTGGATCGTTCTACCTGGTCAATTTGATCTTGGCCGTGGTCACCAT GGCGTATGAAGAGCAGAGCCAGGCAACAATTGCAGAAATCGAAGCCAAGGAAAAAAAGTTCCAGGAAGCC CTTGAGGTGCTGCAGAAGGAACAGGAGGTGCTGGAAGCCCTGGGGATTGACACGACCTCGCTCCAGTCCC ACAGTGGATCACCCTTAGCCTCCAAAAACGCCAATGAGAGAAGACCCAGGGTGAAATCAAGGGTGTCAGA GGGCTCCACGGATGACAACAGGTCACCCCAATCTGACCCTTACAACCAGCGCAGGATGTCTTTCCTAGGC CTGTCTTCAGGAAGACGCAGGGCTAGCCACGGCAGTGTGTTCCACTTCCGAGCGCCCAGCCAAGACATCT CATTTCCTGACGGGATCACCCCTGATGATGGGGTCTTTCACGGAGACCAGGAAAGCCGTCGAGGTTCCAT ATTGCTGGGCAGGGGTGCTGGGCAGACAGGTCCACTCCCCAGGAGCCCACTGCCTCAGTCCCCCAACCCT GGCCGTAGACATGGAGAAGAGGGACAGCTCGGAGTGCCCACTGGTGAGCTTACCGCTGGAGCGCCTGAAG GCCCGGCACTGCACACTACAGGGCAGAAGAGCTTCCTGTCTGCGGGCTACTTGAACGAACCTTTCCGAGC ACAGAGGGCCATGAGCGTTGTCAGTATCATGACTTCTGTCATTGAGGAGCTTGAAGAGTCTAAGCTGAAG TGCCCACCCTGCTTGATCAGCTTCGCTCAGAAGTATCTGATCTGGGAGTGCTGCCCCAAGTGGAGGAAGT TCAAGATGGCGCTGTTCGAGCTGGTGACTGACCCCTTCGCAGAGCTTACCATCACCCTCTGCATCGTGGT GAACACCGTCTTCATGGCCATGGAGCACTACCCCATGACCGATGCCTTCGATGCCATGCTTCAAGCCGGC AACATTGTCTTCACCGTGTTTTTCACAATGGAGATGGCCTTCAAGATCATTGCCTTCGACCCCTACTATT ACTTCCAGAAGAAGTGGAATATCTTCGACTGTGTCATCGTCACCGTGAGCCTTCTGGAGCTGAGTGCATC CAAGAAGGGCAGCCTGTCTGTGCTCCGTACCTTACGCTTGCTGCGGGTCTTCAAGCTGGCCAAGTCCTGG CCCACCCTGAACACCCTCATCAAGATCATCGGGAACTCAGTGGGGGCCCTGGGCAACCTGACCTTTATCC TGGCCATCATCGTCTTCATCTTCGCCCTGGTCGGAAAGCAGCTTCTCTCAGAGGACTACGGGTGCCGCAA GGACGGCGTCTCCGTGTGGAACGGCGAGAAGCTCCGCTGGCACATGTGTGACTTCTTCCATTCCTTCCTG GTCGTCTTCCGAATCCTCTGCGGGGAGTGGATCGAGAACATGTGGGTCTGCATGGAGGTCAGCCAGAAAT CCATCTGCCTCATCCTCTTCTTGACTGTGATGGTGCTGGGCAACCTAGTGGTGCTCAACCTTTTCATCGC TTTACTGCTGAACTCCTTCAGCGCGGACAACCTCACGGCTCCAGAGGATGACGGGGAGGTGAACAACTTG CAGTTAGCACTGGCCAGGATCCAGGTACTTGGCCATCGGGCCAGCAGGGCCATCGCCAGTTACATCAGCA GCCACTGCCGATTCCACTGGCCCAAGGTGGAGACCCAGCTGGGCATGAAGCCCCCACTCACCAGCTCAGA GGCCAAGAACCACATTGCCACTGATGCTGTCAGTGCTGCAGTGGGGAACCTGACAAAGCCAGCTCTCAGT AGCCCCAAGGAGAACCACGGGGACTTCATCACTGATCCCAACGTGTGGGTCTCTGTGCCCATTGCTGAGG GGGAATCTGACCTCGACGAGCTCGAGGAAGATATGGAGCAGGCTTCGCAGAGCTCCTGGCAGGAAGAGGA CCCCAAGGGACAGCAGGAGCAGTTGCCACAAGTCCAAAAGTGTGAAAACCACCAGGCAGCCAGAAGCCCA GCCTCCATGATGTCCTCTGAGGACCTGGCTCCATACCTGGGTGAGAGCTGGAAGAGGAAGGATAGCCCTC AGGTCCCTGCCGAGGGAGTGGATGACACGAGCTCCTCTGAGGGCAGCACGGTGGACTGCCCGGACCCAGA GGAAATCCTGAGGAAGATCCCCGAGCTGGCAGATGACCTGGACGAGCCCGATGACTGTTTCACAGAAGGC TGCACTCGCCGCTGTCCCTGCTGCAACGTGAATACTAGCAAGTCTCCTTGGGCCACAGGCTGGCAGGTGC GCAAGACCTGCTACCGCATCGTGGAGCACAGCTGGTTTGAGAGTTTCATCATCTTCATGATCCTGCTCAG CAGTGGAGCGCTGGCCTTTGAGGATAACTACCTGGAAGAGAAACCCCGAGTGAAGTCCGTGCTGGAGTAC ACTGACCGAGTGTTCACCTTCATCTTCGTCTTTGAGATGCTGCTCAAGTGGGTAGCCTATGGCTTCAAAA AGTATTTCACCAATGCCTGGTGCTGGCTGGACTTCCTCATTGTGAACATCTCCCTGACAAGCCTCATAGC GAAGATCCTTGAGTATTCCGACGTGGCGTCCATCAAAGCCCTTCGGACTCTCCGTGCCCTCCGACCGCTG CGGGCTCTGTCTCGATTCGAAGGCATGAGGGTAGTGGTGGATGCCCTCGTGGGCGCCATCCCCTCCATCA TGAACGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCATGGGCGTGAACCTCTTCGCCGG GAAATTTTCGAAGTGCGTCGACACCAGAAATAACCCATTTTCCAACGTGAATTCGACGATGGTGAATAAC AAGTCCGAGTGTCACAATCAAAACAGCACCGGCCACTTCTTCTGGGTCAACGTCAAAGTCAACTTCGACA ACGTCGCTATGGGCTACCTCGCACTTCTTCAGGTGGCAACCTTCAAAGGCTGGATGGACATAATGTATGC AGCTGTTGATTCCGGAGAGATCAACAGTCAGCCTAACTGGGAGAACAACTTGTACATGTACCTGTACTTC GTCGTTTTCATCATTTTCGGTGGCTTCTTCACGCTGAATCTCTTTGTTGGGGTCATAATCGACAACTTCA ACCAACAGAAAAAAAAGCTAGGAGGCCAGGACATCTTCATGACAGAAGAGCAGAAGAAGTACTACAATGC CATGAAGAAGCTGGGCTCCAAGAAACCCCAGAAGCCCATCCCACGGCCCCTGAATAAGTACCAAGGCTTC GTGTTTGACATCGTGACCAGGCAAGCCTTTGACATCATCATCATGGTTCTCATCTGCCTCAACATGATCA CCATGATGGTGGAGACCGACGAGCAGGGCGAGGAGAAGACGAAGGTTCTGGGCAGAATCAACCAGTTCTT TGTGGCCGTCTTCACGGGCGAGTGTGTGATGAAGATGTTCGCCCTGCGACAGTACTACTTCACCAACGGC TGGAACGTGTTCGACTTCATAGTGGTGATCCTGTCCATTGGGAGTCTGCTGTTTTCTGCAATCCTTAAGT CACTGGAAAACTACTTCTCCCCGACGCTCTTCCGGGTCATCCGTCTGGCCAGGATCGGCCGCATCCTCAG GCTGATCCGAGCAGCCAAGGGGATTCGCACGCTGCTCTTCGCCCTCATGATGTCCCTGCCCGCCCTCTTC AACATCGGCCTCCTCCTCTTCCTCGTCATGTTCATCTACTCCATCTTCGGCATGGCCAGCTTCGCTAACG TCGTGGACGAGGCCGGCATCGACGACATGTTCAACTTCAAGACCTTTGGCAACAGCATGCTGTGCCTGTT CCAGATCACCACCTCGGCCGGCTGGGACGGCCTCCTCAGCCCCATCCTCAACACGGGGCCTCCCTACTGC GACCCCAACCTGCCCAACAGCAACGGCTCCCGGGGGAACTGCGGGAGCCCGGCGGTGGGCATCATCTTCT TCACCACCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATCGCAGTGATTCTGGAGAACTT CAACGTAGCCACCGAGGAGAGCACGGAGCCCCTGAGCGAGGACGACTTCGACATGTTCTATGAGACCTGG GAGAAGTTCGACCCGGAGGCCACCCAGTTCATTGCCTTTTCTGCCCTCTCAGACTTCGCGGACACGCTCT CCGGCCCTCTTAGAATCCCCAAACCCAACCAGAATATATTAATCCAGATGGACCTGCCGTTGGTCCCCGG GGATAAGATCCACTGTCTGGACATCCTTTTTGCCTTCACAAAGAACGTCTTGGGAGAATCCGGGGAGTTG GACTCCCTGAAGACCAATATGGAAGAGAAGTTTATGGCGACCAATCTCTCCAAAGCATCCTATGAACCAA TAGCCACCACCCTCCGGTGGAAGCAGGAAGACCTCTCAGCCACAGTCATTCAAAAGGCCTACCGGAGCTA CATGCTGCACCGCTCCTTGACACTCTCCAACACCCTGCATGTGCCCAGGGCTGAGGAGGATGGCGTGTCA CTTCCCGGGGAAGGCTACATTACATTCATGGCAAACAGTGGACTCCCGGACAAATCAGAAACTGCCTCTG CTACGTCTTTCCCGCCATCCTATGACAGTGTCACCAGGGGCCTGAGTGACCGGGCCAACATTAACCCATC TAGCTCAATGCAAAATGAAGATGAGGTCGCTGCTAAGGAAGGAAACAGCCCTGGACCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR211430 representing NM_017247 Red=Cloning site Green=Tags(s) MELPFASVGTTNFRRFTPESLAEIEKQIAAHRAAKKARTKHRGQEDKGEKPRPQLDLKDCNQLPKFYGEL PAELVGEPLEDLDPFYSTHRTFMVLNKSRTISRFSATWALWLFSPFNLIRRTAIKVSVHSWFSIFITITI LVNCVCMTRTDLPEKVEYVFTVIYTFEALIKILARGFCLNEFTYLRDPWNWLDFSVITLAYVGAAIDLRG ISGLRTFRVLRALKTVSVIPGLKVIVGALIHSVRKLADVTILTVFCLSVFALVGLQLFKGNLKNKCIRNG TDPHKADNLSSEMAEYIFIKPGTTDPLLCGNGSDAGHCPGGYVCLKTPDNPDFNYTSFDSFAWAFLSLFR LMTQDSWERLYQQTLRASGKMYMVFFVLVIFLGSFYLVNLILAVVTMAYEEQSQATIAEIEAKEKKFQEA LEVLQKEQEVLEALGIDTTSLQSHSGSPLASKNANERRPRVKSRVSEGSTDDNRSPQSDPYNQRRMSFLG LSSGRRRASHGSVFHFRAPSQDISFPDGITPDDGVFHGDQESRRGSILLGRGAGQTGPLPRSPLPQSPNP GRRHGEEGQLGVPTGELTAGAPEGPALHTTGQKSFLSAGYLNEPFRAQRAMSVVSIMTSVIEELEESKLK CPPCLISFAQKYLIWECCPKWRKFKMALFELVTDPFAELTITLCIVVNTVFMAMEHYPMTDAFDAMLQAG NIVFTVFFTMEMAFKIIAFDPYYYFQKKWNIFDCVIVTVSLLELSASKKGSLSVLRTLRLLRVFKLAKSW PTLNTLIKIIGNSVGALGNLTFILAIIVFIFALVGKQLLSEDYGCRKDGVSVWNGEKLRWHMCDFFHSFL VVFRILCGEWIENMWVCMEVSQKSICLILFLTVMVLGNLVVLNLFIALLLNSFSADNLTAPEDDGEVNNL QLALARIQVLGHRASRAIASYISSHCRFHWPKVETQLGMKPPLTSSEAKNHIATDAVSAAVGNLTKPALS SPKENHGDFITDPNVWVSVPIAEGESDLDELEEDMEQASQSSWQEEDPKGQQEQLPQVQKCENHQAARSP ASMMSSEDLAPYLGESWKRKDSPQVPAEGVDDTSSSEGSTVDCPDPEEILRKIPELADDLDEPDDCFTEG CTRRCPCCNVNTSKSPWATGWQVRKTCYRIVEHSWFESFIIFMILLSSGALAFEDNYLEEKPRVKSVLEY TDRVFTFIFVFEMLLKWVAYGFKKYFTNAWCWLDFLIVNISLTSLIAKILEYSDVASIKALRTLRALRPL RALSRFEGMRVVVDALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFSKCVDTRNNPFSNVNSTMVNN KSECHNQNSTGHFFWVNVKVNFDNVAMGYLALLQVATFKGWMDIMYAAVDSGEINSQPNWENNLYMYLYF VVFIIFGGFFTLNLFVGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGF VFDIVTRQAFDIIIMVLICLNMITMMVETDEQGEEKTKVLGRINQFFVAVFTGECVMKMFALRQYYFTNG WNVFDFIVVILSIGSLLFSAILKSLENYFSPTLFRVIRLARIGRILRLIRAAKGIRTLLFALMMSLPALF NIGLLLFLVMFIYSIFGMASFANVVDEAGIDDMFNFKTFGNSMLCLFQITTSAGWDGLLSPILNTGPPYC DPNLPNSNGSRGNCGSPAVGIIFFTTYIIISFLIVVNMYIAVILENFNVATEESTEPLSEDDFDMFYETW EKFDPEATQFIAFSALSDFADTLSGPLRIPKPNQNILIQMDLPLVPGDKIHCLDILFAFTKNVLGESGEL DSLKTNMEEKFMATNLSKASYEPIATTLRWKQEDLSATVIQKAYRSYMLHRSLTLSNTLHVPRAEEDGVS LPGEGYITFMANSGLPDKSETASATSFPPSYDSVTRGLSDRANINPSSSMQNEDEVAAKEGNSPGPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_017247
ORF Size	5871 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_017247.1, NP_058943.1
RefSeq Size	6524 bp
RefSeq ORF	5874 bp
Locus ID	29571
UniProt ID	Q62968
MW	219.9 kDa
Gene Summary	voltage-gated sodium channel that is resistant to tetrodotoxin [RGD, Feb 2006]

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