Plxnb3 (NM_001135878) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1560615
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR212366 representing NM_001135878 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGACTGACTTCCTCCAGGCTCCTGTGATGGCTCCCTGGTCTCCCTTCAGCCTCCACCTTCTGCTGC TGTTCCTGCTGTTGCTGCCCTTGACCAGGGCCCATCGCTTCTCTGTTCCGAACGCCAGCTTCAACCACCT GGTGCTGGCACCAGACCAGGGCAAACTCTATGTGGGTGCAGTGAATCACCTCTTCCAGCTCAGCCCTGAG CTGGAGATGGAGTCTGTGGCTATCACTGGCCCTGTCATTGACAGTCCTGATTGTGTGCCTTTCCGTGACC TGGCTGAGTGCCCACAGGCCCAGCTCACTGACAATGCCAACCAGCTCTTGCTGGTAAGCAGCAGGGCACA GGAGCTGGTGGCTTGTGGACAAGTGAGGCAGGGTGTATGTGAGAAGCGGCGCCTTGGGGATGTTACTCAA GTGCTGTACCAGGCGGAGGACCCTGGTGATGGGCAGTTTGTGGCTGCCAATACCCTGGGAGTGACCACAG TAGGCCTAGTGGTGCCTTTGCCAGGCCGAGACCTCCTGCTGGTGGCCAGAGGCCTGGCAGGCAAGCTGTC AGCAGGAGTGCCACCCCTAACTGTGCGCCAGCTGGCTGGGCCACAGCCTTTCTCCAGCGAAGGTCTGGGA AGACTTGTGGTGGGTGACTTTTCAGACTATAACAATAGCTATGTAGGGGCTTTTTCTGATGCTCATTCTG CCTACTTTGTCTTCCGTCGCCGTGGGGCTCGGGCCCAGACTGAGTATCGCTCCTATGTAGCTCGCGTCTG CCTCGGAGATGTCAACCTTTATTCCTATGTGGAAGTGCCCCTTACCTGCCATGGCCAGGGTCTTATCCAG GCTGCCTTCCTTGCTCCGGACACCTTGCTGGGCGCATTTTCAGCAGGCACAAGTCAGGCACAGGCAGCCC TGTGTGCCTTTCCCTTAGCTGACCTGGACGGGAGTATGGAACAAGCCAGGCGACTTTGCTATACTACTGG TGGTCAGGGTCCCAACGGCATGGAAGAAGCCACTGTGGAGTATGGTGTCACATCCCGTTGTGTCACTCTG CCTCCTGACTCCCCCGAGTCATACCCCTGTGGTGATGAACACACCCCCAGCCCCATCGCAGGCCGCCAAC CCCTGGAGGCCCAGCCTCTGCTGCAGCTGGGACAGCCCATCAGTGCAGTGGCTGCTCTCCAGACAGATGG GCACACGATAGCCTTCCTGGGAGACACCGAGGGTCAACTACATAAGGTCTTTCTAAACAGCTCCCATGGC CAGGTATACCATTCCCAGCAAGTGGGGCCCCCAGGCTCAGCCATCAGCCCAGACCTGCTGGTAGACAACA GTGGAGACTACCTCTATGTCCTGACTGCCCAGCAGGTAGACAGGATACTTGTGGCAGCCTGCCCTCAGTT TCCTAACTGCACTACCTGCCTCCAGGCCAGGGACCCACTGTGTGGCTGGTGCATACTTCAGGGCAGGTGC ACTCGGAGGGCCGAGTGTGGGAGAGCAGTTCAGCCAAACCAGTGGCTATGGAGCTATGAAGACAACCACT GCCTTCACATCCAGAGCCTGCTACCAGCCCAGCACCCCCGTCAGGAACATGGCCAGATCACTCTGTCTGT TCCTGGGCTGCCCAACCTGGCCATGGATGAATACTTCTACTGTGCCTTTGGGGACTACAACAGTCTAGCT CAGGTGGAAGAGCACCATGTGGTCTGTGCCACCCCTCCTCAAGATCGGATGCCACCTAATCCTCCAGGCT CAGATCATGTCACCTTGCCCCTGGCCCTGATGTTTGAGGATGTGGTATTGGCTGCCACCACATTCTCCTT CTATGACTGCAGCGCTATTCAGGCCTTGGAAGTGGCTGCCCCGTGCCGCACTTGTGTGAGCAGCCTGTGG CGGTGCCACTGGTGCCCCCAGAGTAGCCACTGTGTATATGGAGAGCGCTGCCCAGAGGGTGAAAAGGCTG TCTACAGTGCCCAGGAGGTGGACATCCTGGTTCGTGGCCCAGAGGCTTGCCCCCAGGTTAAGGGTCTAGC AAGTCCCCAGTTGGTGCCTGTGGGCTGGGAGAGCCATGTGACCCTGCACATTGAGAACCTTCACTATTTC CGAGGTCTGCCTGCCTTGTACTATTGCTGGCTAGAACTGCCTGGAAAACTGCGAAAGCTACCTGCCTTCC TAGAGGAGACATCCAGGAACTCTGGCCTCATTCACTGCCAGGCCCAGCAGTTCCACCCCTCTATGTCTCA GTGGGAGCTCCCAGTGCCCATCTATGTAACCAGGGGTGAGATCCAGCGGCTGGACAATACCGGTGATCTT CATGTGACTCTATATGACTGTGCCATGGGTCACCCTGACTGCAGCCACTGTCAGGCAGCCAATGGGAGCC TGAGCTGCTTGTGGTGTGGTGATGGCCAGCCTGCCTGCCGCTACGGGCCGCTGTGCCCACCAGGTGCTGT GGAACAGCTATGCCCTATACCCAGCATTGATGTGATTGAACCCCTGACTGGTCCCCCAGAAGGTGGCTTA GCCATCACCATCCTGGGATCCAACCTGGGCCAGGCCTTTAATGATGTGCGAAATGCTGTGACTGTGGCTG GCCAGCCCTGCAACCCTGACCCTTCTCTCTACCGCATCTCTGCCCGGATTGTATGTGTCACATCTCCTGC TCCCAATGGCACTTCTGGACCAGTCCAAGTGGCCATTAAGAGTCGTCCTCCTGGCATCTCGGCCCAGCAC TTCACCTACCAGGACCCTGTCCTGCTGAGCCTGAATCCTCAGTGGGGCCCCCAGGCAGGGGGCACCCAGC TCACCATTCATGGGCAGTACCTCCAGACAGGAGGCAACGTCAGTGCTTTTGTGGGGGACCAACCTTGCCC TATTCAAGAGCCTGTGTGTCCTGAAGCCATCATATGCCACACCATGCCCCAGATGGAGCCAGGAGAAGCA GTGGTCTTCGTAGTCTTTGGCCATGTTGAACGCAAACTGCTCACCACTCCTTTTCGATACACTGCCAACC CCCAACTGGTGGAAGCAGAGCCTAGTGTCAGCTTCCGAGGAGGTGGACGAGTGATTCGAGTTAGGGGTAC AGGCTTAGATGTTGTGTGGCAGCCCTTGCTGTCTGTGTGGCTGGAGGATGAGTTCCAAGTGAAGGCTTTG GGCGTGCAGGCCCAGGATGTGAACCCAAGGAGGAGCTGTGGTGCCCCTGCTGCAGATCCTCAGGCTTGTA TCCATCTTGAGAGTGGCTTACTACAGTGCTCCACTCTCTGCTCTGTCAACTCATCTAGTCTCCTCCTGTG CCACAGTCCAGCGGTGCCAGATGGGGCCCTCCCAAAGCGAGTCTTCTTTGCCCTAGACAACATGCAAGTA GACTTTGCCAGTGCTAGTGGGGGCCAGGGATTCCTCTACCAGCCTAACCCTCGCCTGGCTCCACTTAGTC ATGAGGGGATTACTCACCCCTATCGGCTCAAGCCAGGTCATGTCCTGGATGTGGAGGGCGAGGGCCTCAA TCTGGGGATCAGCAAGGAGGAGGTGCAGGTGCATATTGGAGATGGGAAGTGCCTGGTGAAGACGCTGACG CTTACTCACTTGTACTGTGAACCACCCCAGCAAGCCCCTCAGCCCACCAATGGTTCAGGCACCCTGCCAC AGTTTGTGGTGCAGATGGGCAATGTGCGCCTGGCTCTAGGCCCTGTTCAGTATGAAGCAGAGCCCATGAT ATCCACCTTTCCTGTGGAGGCCCAGGTGGGCCTGGGCATGGGTGCTGCCATGCTGATTGCTGCAGTGCTA CTCCTCACCCTCATGTACAGGCACAAGAGCAAGCAGGCCTTGCGAGACTATCAGAAGGTACTGGTGCAGT TGGAGAACCTGGAGACTGGTGTGGGTGACCAGTGCCGCAAGGAGTTCACAGACCTGATGACTGAGATGAC AGACCTCACCAGTGACCTAGAGGCCAGTGGGATTCCCTTCCTGGATTACCGAACATATGCTGAGCGTGCT TTCTTCCCTGGCCATGTTGGCTGCCCACTGCAGCCAGGGCTCGAGGGGCCTGGGGAAGAGGGTCGCCGTG TCACCGTGTGCCAGGGTCTCACACAACTCTCCAATTTGCTCAACAGCAAGCTCTTCCTTCTCACACTCAT CCACACCTTAGAGGAGCAGCCAAGCTTTTCCCAGAGGGACCGCTGCCATGTGGCTTCACTACTTTCTCTG GCTCTGCACAGCAAGCTTGAATATTTGACCGACATCATGAGAACTCTGCTTGGTGACCTAGCAGCCCATT ATGTACACAAGAACCCCAAGCTCATGTTACGCAGGACAGAGACCATGGTAGAGAAGCTGCTCACCAACTG GCTATCTATCTGTCTCTACGCCTTCTTGAAGGAAGTGGCTGGTGAGCCACTGTACATGCTCTTCCGGGCC ATCAAGTACCAGGTGGACAAGGGCCCTGTGGACGCTGTGACAGGCAAGGCAAAACGAACTCTGAATGACA GCCACCTACTACGAGAGGATGTGGAGTTCCGGCCCCTGACCCTGATGGCATTGGTGGGACCAGGGGCTGG TGGGGCAGCAGGAAACAGTGAAGTACATCGAGTGCCAGCCCGGGTGCTTGATACAGACACCATCACCCAA GTCAAAGAGAAGGTGTTGGATCAAATCTACAAGGGAACTCCCTTCTCCCAGAGGCCTTCAGTGCACTCCC TAGACCTTGAGTGGCGCTCAGGCCTAGCTGGTCACCTAACCTTGTCAGATGAGGATTTGACCTCTGTGAC TCAAAACCACTGGAAGAGACTCAACACCCTACAACACTACAAGGTCCCAGATGGAGCAACAGTGGTACTC ATTCCTCAGCTGCACAATGGTGGCACTGTCTCCCAGAGTCTGGAGCAGACTGGCTGCCATTCTGGAGAGA ACACTCCCATGCTGGAAGATGGTGAAGAAGGTGGAGTTCGGCTTTGGCACCTGGTAAAGGCCACTGAGGA GACAGAAGGGGCCAAAGTGCGGCGCAGCAGCCTCCGGGAACGAGAACGAGAACGGGCACGAGCCAAGGCC ATTCCTGAAATCTATCTTACTCGCTTACTTTCAATGAAGGGCACCCTGCAGAAGTTTGTGGATGACACCT TCCAGGCCATTCTTAGCATGAATAGGCCTGTGCCTATTGCTGTTAAGTACCTATTTGACTTCTTGGATGA GCTGGCAGAGAAACATGGCATTGAAGATCCAGAGACCCTGCACATTTGGAAGACAAACAGCTTATTGCTG CGATTCTGGGTGAATGCCTTGAAGAACCCACAGCTCATCTTTGATGTTCGAGTGTCAGACAACGAGGATG CCATCTTGGCTGTCATCGCACAGACCTTCATCGATTCCTGCATGGTCTCAGAGCATAAAGTGGGCCGGGA TTCCCCAGTGAACAAACTGCTCTATGCCCGGGAGATCCCTCGCTACAAGCAGATGGTGGAGAAGTACTAT GCGGACATTCGCCAGAGCTCTCCAGCGAGCTATCAAGAGATGAACTCAGCTCTGGCTGAACTCTCTGGGA ATTATACTTCTGCTCCCAACTGTCTGGAGGCTCTTCGAGAACTTTACAACCACATCCACAGGTATTATGA CCAGATCATCAGTGCCCTGGAGGAGGACCCCGTGGCCCAGAAGATGCAGCTGGCTTGCCGCCTGCAGCAG GTCGCTGCCTTGGTGGAGTACAAAGTGACTGATTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR212366 representing NM_001135878 Red=Cloning site Green=Tags(s) MLTDFLQAPVMAPWSPFSLHLLLLFLLLLPLTRAHRFSVPNASFNHLVLAPDQGKLYVGAVNHLFQLSPE LEMESVAITGPVIDSPDCVPFRDLAECPQAQLTDNANQLLLVSSRAQELVACGQVRQGVCEKRRLGDVTQ VLYQAEDPGDGQFVAANTLGVTTVGLVVPLPGRDLLLVARGLAGKLSAGVPPLTVRQLAGPQPFSSEGLG RLVVGDFSDYNNSYVGAFSDAHSAYFVFRRRGARAQTEYRSYVARVCLGDVNLYSYVEVPLTCHGQGLIQ AAFLAPDTLLGAFSAGTSQAQAALCAFPLADLDGSMEQARRLCYTTGGQGPNGMEEATVEYGVTSRCVTL PPDSPESYPCGDEHTPSPIAGRQPLEAQPLLQLGQPISAVAALQTDGHTIAFLGDTEGQLHKVFLNSSHG QVYHSQQVGPPGSAISPDLLVDNSGDYLYVLTAQQVDRILVAACPQFPNCTTCLQARDPLCGWCILQGRC TRRAECGRAVQPNQWLWSYEDNHCLHIQSLLPAQHPRQEHGQITLSVPGLPNLAMDEYFYCAFGDYNSLA QVEEHHVVCATPPQDRMPPNPPGSDHVTLPLALMFEDVVLAATTFSFYDCSAIQALEVAAPCRTCVSSLW RCHWCPQSSHCVYGERCPEGEKAVYSAQEVDILVRGPEACPQVKGLASPQLVPVGWESHVTLHIENLHYF RGLPALYYCWLELPGKLRKLPAFLEETSRNSGLIHCQAQQFHPSMSQWELPVPIYVTRGEIQRLDNTGDL HVTLYDCAMGHPDCSHCQAANGSLSCLWCGDGQPACRYGPLCPPGAVEQLCPIPSIDVIEPLTGPPEGGL AITILGSNLGQAFNDVRNAVTVAGQPCNPDPSLYRISARIVCVTSPAPNGTSGPVQVAIKSRPPGISAQH FTYQDPVLLSLNPQWGPQAGGTQLTIHGQYLQTGGNVSAFVGDQPCPIQEPVCPEAIICHTMPQMEPGEA VVFVVFGHVERKLLTTPFRYTANPQLVEAEPSVSFRGGGRVIRVRGTGLDVVWQPLLSVWLEDEFQVKAL GVQAQDVNPRRSCGAPAADPQACIHLESGLLQCSTLCSVNSSSLLLCHSPAVPDGALPKRVFFALDNMQV DFASASGGQGFLYQPNPRLAPLSHEGITHPYRLKPGHVLDVEGEGLNLGISKEEVQVHIGDGKCLVKTLT LTHLYCEPPQQAPQPTNGSGTLPQFVVQMGNVRLALGPVQYEAEPMISTFPVEAQVGLGMGAAMLIAAVL LLTLMYRHKSKQALRDYQKVLVQLENLETGVGDQCRKEFTDLMTEMTDLTSDLEASGIPFLDYRTYAERA FFPGHVGCPLQPGLEGPGEEGRRVTVCQGLTQLSNLLNSKLFLLTLIHTLEEQPSFSQRDRCHVASLLSL ALHSKLEYLTDIMRTLLGDLAAHYVHKNPKLMLRRTETMVEKLLTNWLSICLYAFLKEVAGEPLYMLFRA IKYQVDKGPVDAVTGKAKRTLNDSHLLREDVEFRPLTLMALVGPGAGGAAGNSEVHRVPARVLDTDTITQ VKEKVLDQIYKGTPFSQRPSVHSLDLEWRSGLAGHLTLSDEDLTSVTQNHWKRLNTLQHYKVPDGATVVL IPQLHNGGTVSQSLEQTGCHSGENTPMLEDGEEGGVRLWHLVKATEETEGAKVRRSSLRERERERARAKA IPEIYLTRLLSMKGTLQKFVDDTFQAILSMNRPVPIAVKYLFDFLDELAEKHGIEDPETLHIWKTNSLLL RFWVNALKNPQLIFDVRVSDNEDAILAVIAQTFIDSCMVSEHKVGRDSPVNKLLYAREIPRYKQMVEKYY ADIRQSSPASYQEMNSALAELSGNYTSAPNCLEALRELYNHIHRYYDQIISALEEDPVAQKMQLACRLQQ VAALVEYKVTDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001135878
ORF Size	5706 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001135878.1, NP_001129350.1
RefSeq Size	5950 bp
RefSeq ORF	5709 bp
Locus ID	363517
UniProt ID	D3ZLH5
MW	208.5 kDa
Gene Summary	Receptor for SEMA5A that plays a role in axon guidance, invasive growth and cell migration. Stimulates neurite outgrowth and mediates Ca(2+)/Mg(2+)-dependent cell aggregation. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1.[UniProtKB/Swiss-Prot Function]

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