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Map3k7 (NM_001107920) Rat Tagged ORF Clone
CAT＃: RR212444
- TrueORF®
Map3k7 (Myc-DDK-tagged ORF) - Rat mitogen activated protein kinase kinase kinase 7 (Map3k7), (10 ug)
"NM_001107920" in other vectors (3)
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CNY 6,180.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Tak1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212444 representing NM_001107920
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGACAGCATCCGCCGCCTCGTCCTCCTCCTCGTCTTCTGCCAGTGAGATGATCGAAGCGCCATCGC AGGTCCTTAACTTCGAAGAGATCGACTACAAGGAGATCGAGGTGGAAGAGGTTGTTGGGAGAGGAGCTTT TGGAGTGGTTTGCAAAGCTAAGTGGAGAGCAAAAGATGTTGCAATTAAACAGATTGAAAGTGAATCTGAG AGGAAGGCTTTCATCGTGGAGCTCCGGCAGTTGTCCCGTGTGAACCATCCTAATATTGTAAAGTTGTATG GAGCCTGCTTGAATCCAGTCTGTCTTGTGATGGAGTATGCTGAAGGAGGCTCGTTGTATAATGTGCTGCA CGGTGCTGAACCATTGCCTTATTACACTGCTGCTCATGCCATGAGCTGGTGTCTACAGTGTTCCCAAGGA GTGGCCTACCTGCACAGCATGCAGCCCAAAGCTCTCATTCATAGGGACCTCAAGCCTCCAAACTTGCTGC TGGTTGCAGGAGGGACAGTTCTAAAAATCTGCGATTTTGGTACAGCTTGTGACATCCAGACACACATGAC CAATAATAAAGGGAGTGCTGCTTGGATGGCACCCGAAGTGTTCGAAGGTAGCAATTACAGTGAAAAGTGT GATGTCTTCAGCTGGGGTATTATCCTTTGGGAGGTGATAACACGCCGGAAACCCTTTGATGAGATCGGTG GCCCAGCTTTCAGAATCATGTGGGCTGTTCATAATGGTACTCGACCACCACTGATCAAAAACTTGCCTAA GCCCATTGAGAGCTTGATGACCCGCTGTTGGTCTAAGGACCCTTCTCAGCGCCCTTCAATGGAGGAAATT GTGAAAATAATGACTCACTTGATGCGGTACTTTCCAGGAGCTGATGAGCCGTTACAGTACCCCTGTCAGT ACTCTGATGAAGGGCAGAGCAACTCAGCCACCAGCACAGGCTCATTTATGGACATCGCTTCTACAAATAC CAGTAATAAAAGTGACACAAATATGGAACAAGTCCCTGCCACAAACGACACTATTAAACGCTTGGAGTCA AAACTGTTGAAAAACCAGGCAAAGCAACAGAGTGACTCTGGACGCCTGAGTTTGGGAGCCTCTCGAGGGA GCAGTGTGGAGAGCTTGCCCCCGACTTCTGAGGGCAAGAGGATGAGTGCTGACATGTCTGAAATAGAAGC GAGGATCGTGGCGACTACAGCCTATACCAAGCCTAAACGGGGCCACCGTAAAACCGCTTCATTTGGCAAC ATTCTGGATGTCCCTGAGATCGTCATATCAGGCAACGGGCAACCAAGGCGTAGATCCATCCAAGACTTGA CTGTTACTGGGACAGAACCTGGTCAGGTGAGCAGCAGGTCATCCAGCCCTAGTGTCAGAATGATCACTAC CTCAGGACCAACCTCAGAGAAGCCAGCTCGCAGTCTTCCTTGGACCCCTGATGATTCCACAGATACCAAT GGCTCGGATAACTCCATCCCAATGGCGTATCTTACACTGGATCACCAGCTGCAGCCTCTAGCACCATGCC CAAACTCCAAAGAATCCATGGCAGTGTTCGAACAGCATTGTAAAATGGCACAGGAATATATGAAAGTTCA AACCGAAATCGCATTGTTACTACAGAGGAAGCAAGAACTAGTTGCAGAATTGGACCAGGATGAAAAGGAC CAGCAAAATACATCTCGCCTGGTACAGGAACATAAAAAGCTTTTAGACGAAAACAAAAGCCTTTCTACTT ATTACCAGCAATGCAAAAAACAACTAGAGGTCATCAGAAGCCAGCAGCAGAAACGACAAGGCACTTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212444 representing NM_001107920
Red=Cloning site Green=Tags(s) MSTASAASSSSSSSASEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESESE RKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQG VAYLHSMQPKALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKC DVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEI VKIMTHLMRYFPGADEPLQYPCQYSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLES KLLKNQAKQQSDSGRLSLGASRGSSVESLPPTSEGKRMSADMSEIEARIVATTAYTKPKRGHRKTASFGN ILDVPEIVISGNGQPRRRSIQDLTVTGTEPGQVSSRSSSPSVRMITTSGPTSEKPARSLPWTPDDSTDTN GSDNSIPMAYLTLDHQLQPLAPCPNSKESMAVFEQHCKMAQEYMKVQTEIALLLQRKQELVAELDQDEKD QQNTSRLVQEHKKLLDENKSLSTYYQQCKKQLEVIRSQQQKRQGTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001107920 |
| ORF Size | 1818 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001107920.2, NP_001101390.2 |
| RefSeq Size | 4540 bp |
| RefSeq ORF | 1821 bp |
| Locus ID | 313121 |
| UniProt ID | P0C8E4 |
| MW | 67.2 kDa |
| Gene Summary | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2-induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B (By similarity). Promotes TRIM5 capsid-specific restriction activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212444 | Map3k7 (untagged ORF) - Rat mitogen activated protein kinase kinase kinase 7 (Map3k7), (10 ug) |
CNY 6,180.00 |
|
| RR212444L3 | Lenti ORF clone of Map3k7 (Myc-DDK-tagged ORF) - Rat mitogen activated protein kinase kinase kinase 7 (Map3k7), (10 ug) |
CNY 7,980.00 |
|
| RR212444L4 | Lenti ORF clone of Map3k7 (mGFP-tagged ORF) - Rat mitogen activated protein kinase kinase kinase 7 (Map3k7), (10 ug) |
CNY 8,840.00 |
