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Dnajc10 (NM_001106486) Rat Tagged ORF Clone
CAT＃: RR212586
- TrueORF®
Dnajc10 (Myc-DDK-tagged ORF) - Rat DnaJ (Hsp40) homolog, subfamily C, member 10 (Dnajc10), (10 ug)
"NM_001106486" in other vectors (3)
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CNY 10,170.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212586 representing NM_001106486
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGTCTGGTTAAACAGAGATGAATTTATCAGAGACGTGAAGCGGATAAGTCTCTGTCTTCTAGTAC TGTATGTGGTTATCGTGGTGGGCACGGATCAGAATTTTTACAGTTTACTTGGAGTGTCTAAAACTGCAAG CAGCAGAGAAATAAGACAAGCCTTTAAGAAATTAGCACTGAAGTTACATCCTGATAAAAACCCGAATAAC CCAAATGCACATGGTGATTTTTTAAAAATAAATAGAGCATATGAAGTACTCAAAGATGAAGATCTGCGGA AAAAGTATGACAAATACGGAGAAAAGGGCCTGGAAGATAATCAGGGAGGCCAGTACGAGAGCTGGAGCTA CTACCGCTACGACTTCGGTATTTACGATGATGATCCTGAGATCATAACATTAGAAAGAAGAGAATTTGAT GCTGCTGTCAACTCGGGAGAACTGTGGTTTGTAAACTTTTACTCCCCGGGATGTTCGCATTGCCATGATT TAGCCCCCACTTGGAGAGAATTTGCTAAAGAAGTGGATGGATTGCTTCGAATCGGAGCTGTCAACTGTGG TGATGACCGGATGCTTTGCCGAATGAAAGGAGTCAACAGTTACCCCAGCCTCTTCATCTTTAGGTCTGGA ATGGCTGCAGTGAAGTACAACGGGGATAGGTCAAAGGAGAGCTTAGTGAGCTTCGCCATGCAGCACGTCA GGACCACAGTGACAGAGCTGTCAACAGGAAATTTTGTTAATGCCATAGAAACTGCCTTTGCTGCTGGTAT TGGCTGGCTGATCACATTCTGTTTTAAAGGAGAAGATTGTTTGACACCACAGACACGACTCCGGCTTAGT GGCATGTTGGATGGCCTTGTTAACGTGGGATGGGTGGACTGTGACACACAGGACAGCCTCTGCAAGAGCC TGGACGCCACAGCCAGCACTACTGCCTATTTCCCTCCTGGAGCCACTTTGAATAACAAAGAAAAATCCAG TGTTTTGTTTCTTAATTCTCTGGATGCTAAGGAAATATACATGGAAATCATACATAATCTTCCAGACTTT GAACTTCTTTCAGCAAATAAACTAGAGGATCGTTTGGCACATCATCGCTGGCTTGTATTTTTTCATTTTG GAAAAAATGAAAATGCAAATGATCCTGAATTGAAAAAACTGAAAACTCTACTTAAAAATGAACATATTCA AGTTGGAAGGTTTGACTGCTCTTCTGCACCAGGCATCTGCTCTGATCTTTATGTTTTCCAGTCTTGTCTA GCAGTGTTTAAAGGACAAGGAACCAAAGAATATGAAATTCATCACGGAAAGAAGATTCTATACGATATAC TTGCTTTTGCCAAAGAAAGTGTTAATTCGCACGTTACCACACTTGGACCTCAGAACTTCCCTGCAAGTGA CAAAGAACCGTGGCTTGTTGACTTCTTTGCCCCTTGGTGTCCGCCATGCCGTGCGCTTTTGCCAGAGTTA CGGAAAGCATCAACACTGCTCTATGGTCAGCTCAAAGTTGGTACCCTAGACTGCACAATTCATGAAGGAC TCTGCAACATGTATAACATCCAAGCTTATCCAACAACAGTGGTGTTCAACCAGTCCAGTGTTCATGAGTA TGAAGGACATCATTCTGCGGAACAGATCCTGGAGTTCATAGAGGATCTCAGAAACCCTTCCGTGGTCTCT CTTACACCCACCACTTTCAATGAACTAGTTAAACAGAGAAAACATGACGAAGTCTGGATGGTTGATTTCT ATTCTCCATGGTGTCATCCCTGTCAGGTCTTAATGCCAGAGTGGAAACGCATGGCCCGGACGTTAACTGG ATTGATCAATGTAGGCAGTGTGGACTGCCAACAGTATCATTCTTTTTGTACTCAAGAAAATGTCCAGAGA TACCCTGAGATAAGATTTTATCCCCAAAAGTCAAGTAGAGCTTATCAGTATCATAGTTACAATGGCTGGA ATAGGGATGCTTATTCCCTGAGAAGCTGGGGTCTCGGGTTTTTACCCCAAGCATCCATAGATTTAACACC TCAGACATTCAACGAAAAAGTTCTACAAGGGAAAACTCACTGGGTGATTGATTTCTATGCTCCTTGGTGT GGACCTTGCCAGAATTTTGCTCCCGAGTTTGAACTCTTGGCTAGGATGATCAAAGGGAAAGTAAAAGCTG GGAAAGTGGACTGTCAGGCTTACCCCCAAACGTGCCAGAAAGCTGGCATCAGAGCCTACCCCAGTGTCAA ACTGTACCTCTATGAAAGAGCAAAGAAAAGTATTTGGGAAGAGCAAATAAATTCCAGAGATGCAAAAACT ATTGCTGCCTTAATTTATGGAAAATTGGAAACGTTCCAAAGCCAAGTAAAGAGAAATAAGGATGAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212586 representing NM_001106486
Red=Cloning site Green=Tags(s) MGVWLNRDEFIRDVKRISLCLLVLYVVIVVGTDQNFYSLLGVSKTASSREIRQAFKKLALKLHPDKNPNN PNAHGDFLKINRAYEVLKDEDLRKKYDKYGEKGLEDNQGGQYESWSYYRYDFGIYDDDPEIITLERREFD AAVNSGELWFVNFYSPGCSHCHDLAPTWREFAKEVDGLLRIGAVNCGDDRMLCRMKGVNSYPSLFIFRSG MAAVKYNGDRSKESLVSFAMQHVRTTVTELSTGNFVNAIETAFAAGIGWLITFCFKGEDCLTPQTRLRLS GMLDGLVNVGWVDCDTQDSLCKSLDATASTTAYFPPGATLNNKEKSSVLFLNSLDAKEIYMEIIHNLPDF ELLSANKLEDRLAHHRWLVFFHFGKNENANDPELKKLKTLLKNEHIQVGRFDCSSAPGICSDLYVFQSCL AVFKGQGTKEYEIHHGKKILYDILAFAKESVNSHVTTLGPQNFPASDKEPWLVDFFAPWCPPCRALLPEL RKASTLLYGQLKVGTLDCTIHEGLCNMYNIQAYPTTVVFNQSSVHEYEGHHSAEQILEFIEDLRNPSVVS LTPTTFNELVKQRKHDEVWMVDFYSPWCHPCQVLMPEWKRMARTLTGLINVGSVDCQQYHSFCTQENVQR YPEIRFYPQKSSRAYQYHSYNGWNRDAYSLRSWGLGFLPQASIDLTPQTFNEKVLQGKTHWVIDFYAPWC GPCQNFAPEFELLARMIKGKVKAGKVDCQAYPQTCQKAGIRAYPSVKLYLYERAKKSIWEEQINSRDAKT IAALIYGKLETFQSQVKRNKDEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001106486 |
| ORF Size | 2379 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001106486.2, NP_001099956.2 |
| RefSeq Size | 4159 bp |
| RefSeq ORF | 2382 bp |
| Locus ID | 295690 |
| UniProt ID | Q498R3 |
| MW | 90.7 kDa |
| Gene Summary | Endoplasmic reticulum disulfide reductase involved both in the correct folding of proteins and degradation of misfolded proteins. Required for efficient folding of proteins in the endoplasmic reticulum by catalyzing the removal of non-native disulfide bonds formed during the folding of proteins, such as LDLR. Also involved in endoplasmic reticulum-associated degradation (ERAD) by reducing incorrect disulfide bonds in misfolded glycoproteins recognized by EDEM1. Interaction with HSPA5 is required its activity, not for the disulfide reductase activity, but to facilitate the release of DNAJC10 from its substrate. Promotes apoptotic signaling pathway in response to endoplasmic reticulum stress (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212586 | Dnajc10 (untagged ORF) - Rat DnaJ (Hsp40) homolog, subfamily C, member 10 (Dnajc10), (10 ug) |
CNY 10,170.00 |
|
| RR212586L3 | Lenti ORF clone of Dnajc10 (Myc-DDK-tagged ORF) - Rat DnaJ (Hsp40) homolog, subfamily C, member 10 (Dnajc10), (10 ug) |
CNY 11,970.00 |
|
| RR212586L4 | Lenti ORF clone of Dnajc10 (mGFP-tagged ORF) - Rat DnaJ (Hsp40) homolog, subfamily C, member 10 (Dnajc10), (10 ug) |
CNY 13,210.00 |
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