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Eif2ak3 (NM_031599) Rat Tagged ORF Clone
CAT＃: RR212846
- TrueORF®
Eif2ak3 (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3), (10 ug)
"NM_031599" in other vectors (3)
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CNY 17,670.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PEK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212846 representing NM_031599
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCGCCACCCAGCCCCGACCGCGCGCGCTGCTGCTGCTGTTCCTGCTGCTGGGCTGCGCGGCGG GGATCTCAGCGGTCGCGCGCGCCCGCAGCTTGCTTGCTCCCACATCGGATACGGCATTTGGCTTGGGGGC AGCAGCCGCCCCTACTTCAGCCGCGCGGGTGCCTGCGGTGGCTACGGCCGAAGTGACCGTGGAAGACGCC GAGGCATTGCCCGCTGCCTCCGGCGAACAGGAGTCACGCGCGACCGAGTCCGATGACGACGTGGAACTGC GGCCTCGCGGCAGGTCCTTAGTAATCATCAGCACTTTAGATGGACGAATTGCCGCACTGGATGCCGAGAA TCATGGGAAAAAGCAGTGGGATTTGGACGTGGGATCTGGTTCCTTGGTTTCATCTAGCCTTAGCAAGCCA GAGGTGTTTGGGAACAAGATGATCATTCCTTCCCTGGACGGAGACCTCTTCCAGTGGGACCGAGACCGAG AGAGCATGGAGGCCGTCCCTTTCACTGTGGAGTCCCTTCTCGAATCTTCATACAAGTTTGGAGATGATGT TGTTCTGGTTGGAGGAAAGTCTCTGACCACATACGGGCTCAGTGCATATAGTGGAAAGCTGAGGTATATC TGTTCTGCCTTGGGATGTCGCCGATGGGATAGTGATGAAATGGAAGAAGAGGAAGACATCTTGCTTCTGC AACGCACGCAGAAGACTGTGCGAGCTGTCGGGCCTCGAAGCGGCAGTGAGAAGTGGAATTTCAGTGTTGG CCACTTTGAACTTCGGTATATTCCAGACATGGAAACTAGAGCCGGATTCATTGAAAGCACCTTTAAACTG GGTGGAAACAAAGAAGACTCTAAAATCATTTCAGATGTGGAAGAACAAGATGTGGACACAGTGATAAAAG TTTCCGTTGCTGATTGGAAGGTCATGGCGTTTAGTAAGAAGGGAGGCCGCCTAGAATGGGAGTACCAGTT TTGTACTCCCATTGCGTCTGCCTGGCTGGTGAGGGATGGTAAAGTCATCCCCATCAGTCTGTTTGATGAC ACAAGTTACACAGCCAATGAGGAAGTTTTGGAAGATGAAGAAGACATTGTAGAGGCTGCTCGGGGAGCCA CAGAGAACAGCGTGTACTTAGGGATGTACAGAGGCCAGCTGTACCTGCAATCATCCGTCAGGGTCTCGGA AAAGTTCCCTACAAGGCCGAAGGCCTTGGAGTCTGTAAATGGAGAAAGTGCCATTATTCCTCTGCCGACG ATCAAATGGAAGCCCTTAATCCATTCTCCGTCTAGGACTCCTGTCTTGGTTGGGTCTGATGAATTTGACA AGTGTCTAAGTAATGATAAATATTCCCATGAAGAATACAGTAATGGTGCGCTTTCAATCCTACAGTATCC ATATGACAACGGTTACTATCTGCCTTATTACAAGAGAGAAAGGAATAAGCGGAGCACGCAGATCACGGTC AGGTTCCTGGACAGCCCGCACTACAGCAAGAACATCCGCAAGAAGGACCCCATCCTCCTCCTGCACTGGT GGAAGGAAATATTTGGAACGATCTTGCTTTGCATTGTGGCCACGACCTTTATCGTGCGCAGGCTTTTCCA TCCTCAGCCCCACAGGCAACGGAAGGAGTCTGAAACTCAGTGCCAAACTGAAAGTAAATACGACTCTGTG AGTGCTGATAACAGCGACAACAGCTGGAACGACATTAAACACTCAGGATACGTGTCCCGATACCTAACAG ATTTTGAGCCAATTCAGTGCATGGGTCGAGGCGGCTTTGGCGTTGTCTTTGAAGCTAAGAACAAAGTAGA TGACTGCAATTACGCCATCAAGAGGATCCGTCTCCCAAACAGGGAGCTGGCACGGGAGAAGGTAATGCGG GAAGTCAAAGCCTTGGCTAAGCTGGAGCACCCAGGCATCGTGAGGTATTTCAATGCGTGGCTGGAAACAC CACCAGAGAAGTGGCAAGAGGAGATGGATGAAATCTGGCTGAAGGACGAAAGCACAGACTGGCCACTCAG CTCCCCTAGCCCAATGGATGCCCCATCTGTTAAGATCCGGCAGATGGATCCTTTCTCTACAAAAGAACAG ATTGAAGTCATAGCTCCTTCTCCTGAAAGAAGTCGGTCTTTCTCAGTGGGCATTTCCTGTGGCCGGACAA GCTCATCTGAGAGCCAGTTCTCTCCCCTGGAGTTCTCAGGGACAGACTGTGGAGACAACAGTGACTCAGA GGACGCAGCCCACAACCTCCAGGACAGCTGCCTGACAGATTGCGACATGGAAGATGGTACAGTGGACGGC GATGATGAGGGACACTCCTTTGAACTTTGTCCTTCTGAAGCTTCTCCCTATACCAGGTCAAGGGAGGGAA CGTCCTCTTCCATAGTGTTTGAAGACTCTGGCTGTGATAATGCGTCCAGTAAGGAGGACCCCAGAATGAA TCGGCTGCATAATGGCCACCATTACGTTAATAAGCTAACTGAGTTCAAGCACTCCAGCAGCAGGTCTTCT TCCGAAGCCACCTTGTCTACCTCTCCTACCAGGCCAACCACCCTAAGTTTAGATTTCACCAGGAACACGG TGGACCGGCTCCAGCCCAGCTCCCCCAAGGTGTACCTGTACATCCAGATGCAGCTGTGCAGGAAGGAGAA CCTTAAAGACTGGATGAACCGGCGCTGCAGCATGGAGGACAGGGAGCACAGAGTGTGCCTGCACATCTTT CTGCAGATCGCAGAGGCAGTGCAGTTTCTGCACAGCAAGGGACTCATGCACAGGGACCTCAAGCCTTCCA ACATATTCTTCACAATGGATGATGTGGTCAAAGTTGGGGACTTTGGGCTAGTGACTGCAATGGACCAGGA TGAAGAAGAGCAGACAGTACTGACTCCAATGCCAGCCTATGCTACACACACGGGACAAGTCGGGACCAAG CTGTACATGAGCCCAGAACAGATTCATGGAAACAACTACTCCCATAAAGTGGACATCTTCTCTTTAGGCT TGATTCTGTTTGAACTCCTTTACCCATTCAGCACTCAGATGGAACGAGTTAGGACTTTAACTGATGTAAG AAATCTCAAGTTTCCACCACTGTTCACTCAGAAATATCCCCAAGAGCATATGATGGTTCAAGACATGCTT TCTCCATCCCCCATGGAGCGGCCTGAAGCCACAGACATCATTGAAAATGCTGTGTTTGAGAACTTGGAGT TTCCTGGGAAAACGGTTCTGAGACAGCGGTCCCGCTCCTTGAGTTCATCCGGAACTAAACATTCCAGACA GCCCAGCAGCACATTCAGCCCACTGCCTGGCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212846 representing NM_031599
Red=Cloning site Green=Tags(s) MERATQPRPRALLLLFLLLGCAAGISAVARARSLLAPTSDTAFGLGAAAAPTSAARVPAVATAEVTVEDA EALPAASGEQESRATESDDDVELRPRGRSLVIISTLDGRIAALDAENHGKKQWDLDVGSGSLVSSSLSKP EVFGNKMIIPSLDGDLFQWDRDRESMEAVPFTVESLLESSYKFGDDVVLVGGKSLTTYGLSAYSGKLRYI CSALGCRRWDSDEMEEEEDILLLQRTQKTVRAVGPRSGSEKWNFSVGHFELRYIPDMETRAGFIESTFKL GGNKEDSKIISDVEEQDVDTVIKVSVADWKVMAFSKKGGRLEWEYQFCTPIASAWLVRDGKVIPISLFDD TSYTANEEVLEDEEDIVEAARGATENSVYLGMYRGQLYLQSSVRVSEKFPTRPKALESVNGESAIIPLPT IKWKPLIHSPSRTPVLVGSDEFDKCLSNDKYSHEEYSNGALSILQYPYDNGYYLPYYKRERNKRSTQITV RFLDSPHYSKNIRKKDPILLLHWWKEIFGTILLCIVATTFIVRRLFHPQPHRQRKESETQCQTESKYDSV SADNSDNSWNDIKHSGYVSRYLTDFEPIQCMGRGGFGVVFEAKNKVDDCNYAIKRIRLPNRELAREKVMR EVKALAKLEHPGIVRYFNAWLETPPEKWQEEMDEIWLKDESTDWPLSSPSPMDAPSVKIRQMDPFSTKEQ IEVIAPSPERSRSFSVGISCGRTSSSESQFSPLEFSGTDCGDNSDSEDAAHNLQDSCLTDCDMEDGTVDG DDEGHSFELCPSEASPYTRSREGTSSSIVFEDSGCDNASSKEDPRMNRLHNGHHYVNKLTEFKHSSSRSS SEATLSTSPTRPTTLSLDFTRNTVDRLQPSSPKVYLYIQMQLCRKENLKDWMNRRCSMEDREHRVCLHIF LQIAEAVQFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYATHTGQVGTK LYMSPEQIHGNNYSHKVDIFSLGLILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPQEHMMVQDML SPSPMERPEATDIIENAVFENLEFPGKTVLRQRSRSLSSSGTKHSRQPSSTFSPLPGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031599 |
| ORF Size | 3324 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031599.2, NP_113787.1 |
| RefSeq Size | 4509 bp |
| RefSeq ORF | 3327 bp |
| Locus ID | 29702 |
| UniProt ID | Q9Z1Z1 |
| MW | 124.8 kDa |
| Gene Summary | kinase that phosphorylates eukaryotic initiation factor 2alpha; has an important role in translational control [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212846 | Eif2ak3 (untagged ORF) - Rat eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3), (10 ug) |
CNY 17,670.00 |
|
| RR212846L3 | Lenti ORF clone of Eif2ak3 (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3), (10 ug) |
CNY 19,380.00 |
|
| RR212846L4 | Lenti ORF clone of Eif2ak3 (mGFP-tagged ORF) - Rat eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3), (10 ug) |
CNY 21,380.00 |
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