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Nkx2-5 (NM_053651) Rat Tagged ORF Clone
CAT＃: RR213005
- TrueORF®
Nkx2 (Myc-DDK-tagged ORF) - Rat NK2 transcription factor related, locus 5 (Drosophila) (Nkx2-5), (10 ug)
"NM_053651" in other vectors (3)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Csx; Nkx2.5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213005 representing NM_053651
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCCAGCCCTGCGCTCACACACACGCCCTTCTCAGTCAAAGACATCCTGAACCTGGAGCAGCAGC AGCGCAGCCTGGCGGCTGGGGACCTGTCTGCGCGCCTCGAGGCCACCCTGGCGCCCGCCTCCTGCATGCT GGCCGCCTTCAAGCCGGACGGCTACTCAGGCCCCGAGGCGGCAGCGCCCGGCCTGGCAGAGCTGCGCGCG GAGCTGGGCCCCGCGCCTTCGCCCCCCAAGTGCTCTCCTGCTTTCCCAACCGCCCCTACATTTTATCCGC GAGCCTACGGTGACCCTGACCCCGCCAAGGACCCTCGGGCGGATAAGAAAGAGCTGTGCGCGCTGCAGAA GGCGGTGGAGCTGGACAAAGCCGAGACAGACGGCGCCGAGAGGCGACGACCACGGCGGCGACGGAAGCCA CGCGTGCTCTTCTCGCAGGCGCAGGTCTATGAGCTGGAGCGGCGCTTCAAGCAACAGCGGTACCTGTCGC CCGCTGAGCGCGACCAACTGGCCAGCGTGCTGAAGCTCACGTCCACGCAGGTCAAGATCTGGTTCCAGAA CCGCCGCTACAAGTGTAAGCGACAGCGGCAGGACCAGACTCTGGAGCTGCTGGGGCCGCCGCCGCCGCCC GCGCGCAGGATCGCGGTGCCGGTGTTGGTGCGCGACGGGAAGCCCTGCCTGGGGGACTCGGCGGCCTACG CTCCCGCCTACGGCCTGGGTCTCAACGCCTACGGCTACAACGCCTACCCCTATCCCGGCTACGGTGGCGC GGCCTGCAGTCCCGCCTACAGCTGCGCCGCGTACCCCGCCGCGCCCCCCGCCGCGCACGCCCCAGCCGCC AGCGCCAACAGCAACTTCGTGAACTTTGGCGTCGGGGACTTGAACACGGTGCAGAGTCCCGGGATGCCAC AGGGCAATTCGGGCGTCTCCACGCTGCACGGCATCCGAGCCTGGAGGGCAATTCGGGCGTCTCCACGCTG CACGGCATCCGAGCCTGG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR213005 representing NM_053651
Red=Cloning site Green=Tags(s) MFPSPALTHTPFSVKDILNLEQQQRSLAAGDLSARLEATLAPASCMLAAFKPDGYSGPEAAAPGLAELRA ELGPAPSPPKCSPAFPTAPTFYPRAYGDPDPAKDPRADKKELCALQKAVELDKAETDGAERRRPRRRRKP RVLFSQAQVYELERRFKQQRYLSPAERDQLASVLKLTSTQVKIWFQNRRYKCKRQRQDQTLELLGPPPPP ARRIAVPVLVRDGKPCLGDSAAYAPAYGLGLNAYGYNAYPYPGYGGAACSPAYSCAAYPAAPPAAHAPAA SANSNFVNFGVGDLNTVQSPGMPQGNSGVSTLHGIRAWRAIRASPRCTASEP SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053651 |
| ORF Size | 954 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 1342 bp |
| RefSeq ORF | 957 bp |
| Locus ID | 114109 |
| UniProt ID | O35767 |
| MW | 34.3 kDa |
| Gene Summary | homeobox transcription factor important for cardiac development [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213005 | Nkx2 (untagged ORF) - Rat NK2 transcription factor related, locus 5 (Drosophila) (Nkx2-5), (10 ug) |
CNY 3,990.00 |
|
| RR213005L3 | Lenti ORF clone of Nkx2 (Myc-DDK-tagged ORF) - Rat NK2 transcription factor related, locus 5 (Drosophila) (Nkx2-5), (10 ug) |
CNY 6,080.00 |
|
| RR213005L4 | Lenti ORF clone of Nkx2 (mGFP-tagged ORF) - Rat NK2 transcription factor related, locus 5 (Drosophila) (Nkx2-5), (10 ug) |
CNY 6,650.00 |
