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Lepr (NM_012596) Rat Tagged ORF Clone
CAT＃: RR213515
- TrueORF®
Lepr (Myc-DDK-tagged ORF) - Rat leptin receptor (Lepr), (10 ug)
"NM_012596" in other vectors (3)
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CNY 8,088.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fa |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213515 representing NM_012596
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTGTCAGAAATTCTATGTGGTTTTGTTACACTGGGAATTTCTGTATGTGATAACTGCACTTAACC TGGCCTATCCAACCTCTCCCTGGAGATTTAAGCTGTTTTGTGCGCCACCGAGTACAACTGATGACTCCTT TCTCTCTCCTGCTGGAGTCCCAAACAATACTTCGTCTTTGAAGGGGGCTTCTGAAGCACTTGTTGAAGCT AAATTTAATTCAACTGGTATCTACGTTTCTGAGTTATCCAAAACCATTTTCCACTGTTGCTTTGGGAATG AGCAAGGTCAAAACTGCTCCGCACTCACAGGCAACACTGAAGGGAAGACGCTGGCTTCAGTGGTGAAGCC TTTAGTTTTCCGCCAACTAGGTGTAAACTGGGACATAGAGTGCTGGATGAAAGGGGACTTGACATTATTC ATCTGTCATATGGAACCATTACTTAAGAACCCCTTCAAGAATTATGACTCTAAGGTTCACCTTTTATATG ATCTGCCTGAAGTTATAGATGATTTGCCTCTGCCCCCACTGAAAGACAGCTTTCAGACTGTCCAGTGCAA CTGCAGTGTTCGGGAATGCGAATGTCATGTACCAGTACCCAGAGCCAAAGTCAACTACGCTCTTCTGATG TATTTAGAAATCACATCTGCTGGTGTGAGTTTTCAGTCACCTCTAATGTCACTGCAGCCCATGCTTGTTG TGAAGCCCGATCCACCGCTGGGTTTGCGTATGGAAGTCACAGATGATGGTAATTTAAAGATTTCATGGGA CAGCCAAACAAAAGCACCATTTCCACTTCAATATCAGGTGAAATATTTAGAGAATTCTACAATCGTAAGA GAGGCTGCTGAAATCGTCTCGGATACATCTCTGCTGGTAGACAGCGTGCTTCCTGGGTCTTCATACGAGG TCCAGGTGAGGAGCAAGAGACTGGATGGCTCAGGAGTCTGGAGTGACTGGAGTTTACCTCAACTCTTTAC CACACAAGATGTCATGTATTTTCCACCCAAAATTCTGACGAGTGTTGGATCCAATGCTTCCTTTTGCTGC ATCTACAAAAATGAGAACCAGACTATCTCCTCAAAACAAATAGTTTGGTGGATGAATCTAGCCGAGAAGA TCCCCGAGACACAGTACAACACTGTGAGTGACCACATTAGCAAAGTCACTTTCTCCAACCTGAAAGCCAC CAGACCTCGAGGGAAGTTTACCTATGATGCAGTGTACTGCTGCAATGAGCAGGCATGCCATCACCGCTAC GCTGAATTATATGTGATCGATGTCAATATCAATATATCATGTGAAACTGACGGGTACTTAACTAAAATGA CTTGCAGATGGTCACCCAGCACAATCCAATCACTAGTGGGAAGCACTGTGCAGTTGAGGTATCACAGGCG CAGCCTGTACTGTCCCGATAATCCATCTATTCGTCCTACATCAGAGCTCAAAAACTGCGTCTTACAGACA GATGGCTTTTATGAATGTGTTTTCCAGCCAATCTTTCTATTATCTGGCTATACAATGTGGATCAGGATCA ACCATTCTTTAGGTTCACTTGACTCTCCACCAACGTGTGTCCTTCCTGACTCCGTAGTAAAACCACTACC TCCATCTAATGTAAAAGCAGAGATTACTATAAACACTGGATTATTGAAAGTATCTTGGGAAAAGCCAGTC TTTCCAGAGAATAACCTTCAGTTCCAGATTCGATATGGCTTAAATGGAAAAGAAATACAATGGAAGACAC ACGAGGTATTCGATGCAAAATCAAAATCGGCCAGCCTGCCAGTGTCAGATCTCTGTGCGGTCTATGTGGT ACAGGTTCGCTGCCGGCGGTTGGATGGACTAGGGTATTGGAGTAATTGGAGCAGTCCAGCCTACACTCTT GTCATGGATGTAAAAGTTCCTATGAGAGGGCCTGAATTCTGGAGAATAATGGATGGGGATATTACTAAAA AGGAGAGAAATGTCACCTTGCTTTGGAAGCCACTGATGAAAAATGACTCACTGTGTAGTGTGAGGAGGTA TGTGGTGAAGCATCGTACTGCCCACAATGGGACATGGTCACAAGATGTGGGAAATCAGACCAATCTCACT TTCCTGTGGGCAGAATCAGCACACACTGTTACAGTTCTGGCCATCAATTCCATCGGTGCCTCCCTTGTGA ATTTTAACCTTACGTTCTCATGGCCCATGAGTAAAGTGAATGCTGTGCAGTCACTCAGTGCTTATCCCCT GAGCAGCAGCTGCGTCATCCTTTCCTGGACACTGTCACCTAATGATTATAGTCTGTTATATCTGGTTATT GAATGGAAGAACCTTAATGATGATGATGGAATGAAGTGGCTTAGAATCCCTTCGAATGTTAACAAGTATT ATATCCATGATAATTTTATTCCTATCGAGAAATATCAGTTTAGTCTTTACCCAGTATTTATGGAAGGAGT TGGAAAACCAAAGATAATTAATGGTTTCACCAAAGATGATATCGCCAAACAGCAAAATGATGCAGGGCTG TATGTCATTGTACCGATAATTATTTCCTCTTGTGTCCTGCTGCTCGGAACACTGTTAATTTCACACCAGA GAATGAAAAAGTTGTTTTGGGACGATGTTCCAAACCCCAAGAATTGTTCCTGGGCACAAGGACTTAATTT CCAAAAGCCTGAAACATTTGAGCATCTTTTTACCAAGCATGCAGAATCAGTGATATTTGGTCCTCTTCTT CTGGAGCCTGAACCAGTTTCAGAAGAAATCAGTGTCGATACAGCTTGGAAAAATAAAGATGAGATGGTAC CAGCAGCTATGGTCTCACTTCTTTTGACCACTCCAGATTCCACAAGGGGTTCTATTTGTATCAGTGACCA GTGTAACAGTGCTAACTTCTCTGGGGCTCAGAGCACCCAGGGAACCTGTGAGGATGAGTGTCAGAGTCAA CCCTCAGTTAAATATGCAACGCTGGTCAGCAACGTGAAAACAGTGGAAACTGATGAAGAGCAAGGGGCTA TACATAGTTCTGTCAGCCAGTGCATCGCCAGGAAACATTCCCCACTGAGACAGTCTTTTTCTAGCAACTC CTGGGAGATAGAGGCCCAGGCATTTTTCCTTTTATCAGATCATCCACCCAATGTGATTTCACCACAACTT TCATTCTCAGGGTTGGATGAGCTTTTGGAACTGGAGGGAAATTTTCCTGAAGAAAATCACGGGGAAAAAT CTGTGTATTATCTAGGAGTCTCCTCAGGAAACAAAAGAGAGAATGATATGCTTTTGACTGATGAGGCAGG GGTATTGTGCCCATTCCCAGCTCACTGTCTGTTCAGTGACATCAGAATCCTCCAGGAGAGTTGTTCACAC TTTGTAGAAAATAATTTGAATTTAGGGACCTCTGGTAAGAACTTTGTACCTTACATGCCCCAGTTTCAAT CCTGTTCCACTCACAGTCATAAGATAATAGAAAATAAGATGTGTGACTTAACTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213515 representing NM_012596
Red=Cloning site Green=Tags(s) MTCQKFYVVLLHWEFLYVITALNLAYPTSPWRFKLFCAPPSTTDDSFLSPAGVPNNTSSLKGASEALVEA KFNSTGIYVSELSKTIFHCCFGNEQGQNCSALTGNTEGKTLASVVKPLVFRQLGVNWDIECWMKGDLTLF ICHMEPLLKNPFKNYDSKVHLLYDLPEVIDDLPLPPLKDSFQTVQCNCSVRECECHVPVPRAKVNYALLM YLEITSAGVSFQSPLMSLQPMLVVKPDPPLGLRMEVTDDGNLKISWDSQTKAPFPLQYQVKYLENSTIVR EAAEIVSDTSLLVDSVLPGSSYEVQVRSKRLDGSGVWSDWSLPQLFTTQDVMYFPPKILTSVGSNASFCC IYKNENQTISSKQIVWWMNLAEKIPETQYNTVSDHISKVTFSNLKATRPRGKFTYDAVYCCNEQACHHRY AELYVIDVNINISCETDGYLTKMTCRWSPSTIQSLVGSTVQLRYHRRSLYCPDNPSIRPTSELKNCVLQT DGFYECVFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSNVKAEITINTGLLKVSWEKPV FPENNLQFQIRYGLNGKEIQWKTHEVFDAKSKSASLPVSDLCAVYVVQVRCRRLDGLGYWSNWSSPAYTL VMDVKVPMRGPEFWRIMDGDITKKERNVTLLWKPLMKNDSLCSVRRYVVKHRTAHNGTWSQDVGNQTNLT FLWAESAHTVTVLAINSIGASLVNFNLTFSWPMSKVNAVQSLSAYPLSSSCVILSWTLSPNDYSLLYLVI EWKNLNDDDGMKWLRIPSNVNKYYIHDNFIPIEKYQFSLYPVFMEGVGKPKIINGFTKDDIAKQQNDAGL YVIVPIIISSCVLLLGTLLISHQRMKKLFWDDVPNPKNCSWAQGLNFQKPETFEHLFTKHAESVIFGPLL LEPEPVSEEISVDTAWKNKDEMVPAAMVSLLLTTPDSTRGSICISDQCNSANFSGAQSTQGTCEDECQSQ PSVKYATLVSNVKTVETDEEQGAIHSSVSQCIARKHSPLRQSFSSNSWEIEAQAFFLLSDHPPNVISPQL SFSGLDELLELEGNFPEENHGEKSVYYLGVSSGNKRENDMLLTDEAGVLCPFPAHCLFSDIRILQESCSH FVENNLNLGTSGKNFVPYMPQFQSCSTHSHKIIENKMCDLTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012596 |
| ORF Size | 3486 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012596.1, NP_036728.1 |
| RefSeq Size | 3650 bp |
| RefSeq ORF | 3489 bp |
| Locus ID | 24536 |
| UniProt ID | Q62959 |
| MW | 130.8 kDa |
| Gene Summary | receptor for leptin; may have a role in body weight maintainence [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213515 | Lepr (untagged ORF) - Rat leptin receptor (Lepr), (10 ug) |
CNY 8,096.00 |
|
| RR213515L3 | Lenti ORF clone of Lepr (Myc-DDK-tagged ORF) - Rat leptin receptor (Lepr), (10 ug) |
CNY 20,140.00 |
|
| RR213515L4 | Lenti ORF clone of Lepr (mGFP-tagged ORF) - Rat leptin receptor (Lepr), (10 ug) |
CNY 10,488.00 |
