Smc1a (NM_031683) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SB1.8; SMC-1A; Smc1l1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214207 representing NM_031683 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTTCCTGAAACTGATTGAGATTGAGAACTTTAAATCGTACAAGGGTCGACAAATTATCGGACCAT TTCAGAGGTTCACCGCCATCATTGGACCCAATGGCTCTGGTAAGTCAAATCTCATGGATGCCATCAGCTT TGTGCTTGGTGAAAAAACCAGCAACCTGAGGGTAAAGACCCTGAGGGACCTGATACACGGAGCTCCTGTG GGCAAGCCGGCTGCCAACCGAGCCTTTGTCAGCATGGTCTACTCCGAGGAGGGTGCTGAGGACCGCACCT TTGCCCGAGTCATTGTAGGGGGCTCTTCTGAGTACAAGATCAATAACAAAGTTGTCCAGCTACATGAATA CAGTGAAGAGTTAGAGAAGTTGGGCATTCTCATCAAAGCTCGTAACTTCCTCGTCTTCCAGGGTGCAGTG GAATCTATTGCCATGAAGAACCCCAAGGAGAGGACAGCTTTATTTGAAGAAATTAGTCGCTCTGGGGAAC TTGCACAGGAGTATGACAAGCGAAAGAAAGAAATGGTGAAGGCTGAAGAAGACACACAGTTTAATTATCA TCGCAAGAAAAATATTGCAGCTGAACGCAAGGAGGCCAAACAAGAAAAGGAAGAGGCTGACCGCTACCAG GCCCTGAAGGATGAGGTAGTACGCGCACAGGTACAATTGCAGCTTTTTAAACTCTACCACAACGAAGTGG AGATTGAGAAGCTCAACAAGGAACTGGCCTCGAAGAACAAGGAAATTGAGAAGGACAAAAAGCGGATGGA CAAGGTAGAGGATGAACTGAAGGAGAAGAAGAAAGAGCTGGGCAAAATGATGCGGGAACAGCAGCAGATT GAGAAGGAGATCAAGGAAAAGGACTCAGAATTGAATCAGAAGAGGCCTCAGTACATCAAAGCAAAAGAGA ATACATCCCACAAAATCAAGAAGCTTGAAGCAGCCAAGAAATCTCTGCAGAATCGTCAGAAACATTATAA GAAACGTAAAGGTGACATGGATGAGCTGGAGAAAGAGATGCTGTCAGTGGAGAAGGCCCGACAGGAGTTT GAAGAGCGGATGGAGGAAGAGAGTCAGAGTCAAGGCAGAGACTTGACCCTGGAGGAGAACCAGGTAAAGA AATACCACCGATTAAAAGAGGAAGCTAGCAAGAGAGCAGCCACTTTGGCTCAGGAGCTGGAGAAGTTTAA TCGAGACCAGAAAGCTGACCAGGACCGTCTAGATCTAGAAGAGCGGAAGAAAGTAGAGACAGAGGCCAAG ATCAAGCAAAAACTGCGGGAGATTGAAGAGAATCAGAAACGGATTGAGAAGCTAGAGGAATATATTACCA CTAGCAAGCAGTCTCTAGAGGAACAGAAGAAGCTAGAGGGAGAATTGACAGAAGAAGTAGAGATGGCCAA GCGTCGTATTGATGAGATCAATAAGGAGCTGAACCAGGTGATGGAGCAATTGGGGGATGCGCGCATTGAC CGGCAGGAGAGCAGCCGCCAGCAGAGGAAGGCAGAAATAATGGAAAGCATCAAGCGTCTCTACCCTGGTT CTGTGTATGGCCGCCTCATTGACCTCTGCCAGCCTACACAAAAGAAGTATCAGATTGCTGTGACCAAGGT TTTAGGAAAGAACATGGATGCTATTATTGTAGACTCCGAGAAGACAGGTCGGGATTGTATTCAGTATATC AAGGAGCAACGTGGAGAGCCTGAGACCTTCTTGCCTCTTGACTACCTGGAGGTGAAACCTACTGATGAGA AACTCCGAGAACTGAAGGGGGCCAAGCTAGTGATTGATGTGATTCGCTATGAGCCACCTCACATCAAAAA AGCTCTGCAGTATGCTTGTGGCAATGCCCTTGTTTGTGACAATGTGGAGGATGCGCGACGAATTGCCTTT GGAGGTCACCAACGCCACAAGACAGTGGCATTGGATGGCACCCTGTTCCAGAAGTCAGGAGTTATCTCTG GTGGAGCCAGTGATCTCAAGGCAAAGGCACGACGCTGGGATGAGAAGGCAGTAGACAAACTAAAAGAAAA AAAAGAACGTTTGACGGAGGAGCTAAAAGAGCAGATGAAGGCAAAACGAAAAGAGGCAGAGCTGCGTCAG GTGCAGTCTCAGGCCCATGGGCTACAAATGCGGCTGAAGTACTCACAGAGTGACCTAGAACAGACTAAGA CAAGGCATCTTGCCCTGAATCTCCAGGAAAAATCTAAGCTGGAGAGTGAACTAGCCAACTTCGGGCCTCG AATTAATGATATCAAGAGAATCATACAGAGCCGAGAGAGGGAAATGAAAGACTTGAAGGAGAAGATGAAC CAGGTGGAAGATGAGGTGTTTGAAGAGTTTTGTCGGGAGATTGGTGTGCGTAACATCCGCGAATTTGAGG AAGAAAAGGTGAAGCGGCAGAATGAAATTGCCAAGAAACGCTTGGAATTTGAGAATCAGAAGACTCGCTT GGGCATCCAGTTGGATTTTGAAAAGAATCAGTTGAAGGAGGACCAGGATAAAGTACACATGTGGGAGCAG ACAGTAAAAAAGGATGAAAATGAGATAGAAAAGCTTAAGAAGGAGGAGCAAAGACACATGAAGATAATAG ATGAAACCATGGCTCAGCTCCAAGACCTTAAGAATCAGCACCTGGCCAAAAAGTCAGAAGTGAATGACAA GAATCATGAGATGGAAGAAATTCGCAAGAAGCTTGGGGGTGCCAATAAGGAAATGACTCACTTACAGAAG GAAGTGACAGCCATTGAGACCAAGCTTGAACAGAAGCGCAGTGACCGCCACAACCTGCTACAGGCCTGTA AGATGCAGGACATCAAATTGCCACTGTCAAAGGGCACCATGGATGATATTAGTCAGGAAGAAGGTGGCTC CCAGGGAGAAGAGTCAGTGAGTGGTTCCCAGAGAACATCTAGTATCTATGCACGAGAGGCCCTCATTGAG ATTGACTACGGTGACCTGTGTGAAGATCTGAAGGATGCACAGGCGGAAGAAGAAATCAAGCAAGAGATGA ACACACTCCAGCAAAAGCTGAATGAGCAGCAGAGTGTGCTTCAGAGGATTGCTGCCCCTAACATGAAGGC CATGGAAAAGCTGGAAAGTGTCCGAGACAAGTTCCAGGAGACCTCAGATGAATTTGAGGCAGCACGAAAG CGAGCAAAGAAAGCCAAGCAGGCATTTGAACAGATAAAGAAGGAGCGCTTTGACCGATTCAATGCTTGTT TTGAATCTGTGGCTACCAATATTGATGAGATCTACAAGGCTCTGTCCCGTAACAGCAGTGCCCAGGCATT CTTGGGCCCCGAGAATCCTGAAGAGCCCTATTTGGATGGTATCAACTACAACTGTGTAGCTCCTGGCAAA CGTTTCCGGCCTATGGACAATTTGTCAGGCGGGGAGAAGACAGTTGCCGCACTGGCCTTGCTTTTTGCCA TTCACAGCTACAAGCCAGCCCCCTTCTTCGTCCTGGATGAGATTGATGCTGCCTTGGATAACACCAACAT TGGCAAGGTGGCAAATTACATCAAGGAGCAGTCAACTTGCAACTTCCAGGCCATTGTTATCTCTCTCAAG GAGGAATTCTACACCAAGGCTGAGAGCCTCATTGGAGTCTATCCTGAGCAAGGAGACTGTGTGATCAGCA AAGTCCTGACCTTCGACCTCACCAAGTATCCAGATGCCAACCCTAACCCCAATGAACAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214207 representing NM_031683 Red=Cloning site Green=Tags(s) MGFLKLIEIENFKSYKGRQIIGPFQRFTAIIGPNGSGKSNLMDAISFVLGEKTSNLRVKTLRDLIHGAPV GKPAANRAFVSMVYSEEGAEDRTFARVIVGGSSEYKINNKVVQLHEYSEELEKLGILIKARNFLVFQGAV ESIAMKNPKERTALFEEISRSGELAQEYDKRKKEMVKAEEDTQFNYHRKKNIAAERKEAKQEKEEADRYQ ALKDEVVRAQVQLQLFKLYHNEVEIEKLNKELASKNKEIEKDKKRMDKVEDELKEKKKELGKMMREQQQI EKEIKEKDSELNQKRPQYIKAKENTSHKIKKLEAAKKSLQNRQKHYKKRKGDMDELEKEMLSVEKARQEF EERMEEESQSQGRDLTLEENQVKKYHRLKEEASKRAATLAQELEKFNRDQKADQDRLDLEERKKVETEAK IKQKLREIEENQKRIEKLEEYITTSKQSLEEQKKLEGELTEEVEMAKRRIDEINKELNQVMEQLGDARID RQESSRQQRKAEIMESIKRLYPGSVYGRLIDLCQPTQKKYQIAVTKVLGKNMDAIIVDSEKTGRDCIQYI KEQRGEPETFLPLDYLEVKPTDEKLRELKGAKLVIDVIRYEPPHIKKALQYACGNALVCDNVEDARRIAF GGHQRHKTVALDGTLFQKSGVISGGASDLKAKARRWDEKAVDKLKEKKERLTEELKEQMKAKRKEAELRQ VQSQAHGLQMRLKYSQSDLEQTKTRHLALNLQEKSKLESELANFGPRINDIKRIIQSREREMKDLKEKMN QVEDEVFEEFCREIGVRNIREFEEEKVKRQNEIAKKRLEFENQKTRLGIQLDFEKNQLKEDQDKVHMWEQ TVKKDENEIEKLKKEEQRHMKIIDETMAQLQDLKNQHLAKKSEVNDKNHEMEEIRKKLGGANKEMTHLQK EVTAIETKLEQKRSDRHNLLQACKMQDIKLPLSKGTMDDISQEEGGSQGEESVSGSQRTSSIYAREALIE IDYGDLCEDLKDAQAEEEIKQEMNTLQQKLNEQQSVLQRIAAPNMKAMEKLESVRDKFQETSDEFEAARK RAKKAKQAFEQIKKERFDRFNACFESVATNIDEIYKALSRNSSAQAFLGPENPEEPYLDGINYNCVAPGK RFRPMDNLSGGEKTVAALALLFAIHSYKPAPFFVLDEIDAALDNTNIGKVANYIKEQSTCNFQAIVISLK EEFYTKAESLIGVYPEQGDCVISKVLTFDLTKYPDANPNPNEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031683
ORF Size	3699 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031683.1, NP_113871.1
RefSeq Size	3933 bp
RefSeq ORF	3702 bp
Locus ID	63996
UniProt ID	Q9Z1M9
MW	143.2 kDa
Gene Summary	structural maintenance of chromosomes (SMC) protein, one of 2 component proteins forming the cohesin multiprotein complex required for sister chromatid cohesion; thought to be a component of functional kinetochores [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.