Nedd4 (NM_012986) Rat Tagged ORF Clone

CAT＃: RR214217

  • TrueORF®

Nedd4 (Myc-DDK-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated gene 4 (Nedd4), (10 ug)

Datasheet
SDS
Plasmid Map

  "NM_012986" in other vectors (3)

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CNY 11,500.00

货期*
7周
规格
    • 10 ug

Product images

经常一起买 (4)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Nedd4a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR214217 representing NM_012986
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTCGGACATGGCAGCCGACGACACTGAGGCCCCAGTGCTTTCGGAGGACGAGGTATGGGAGTTCT
GTCTGGATAAGAATGAAGAGGGTGGTGGATCCCCCGGAAGTGATGTCACAGATACTTGTGAGCCACCATG
TGGATGCTGGGAGTTAAATCCGAGTTCCCTGGAAGAGGAGCACGTGCTTTTCACTGCCGATCCATATCTC
GAGCTTCACAATGATGACACGCGAGTTGTGAGAGTGAAGGTTATAGCTGGCATAGGTCTGGCCAAGAAAG
ACATCTTGGGAGCCAGTGATCCTTATGTAAGAGTGACATTGTATGACCCGATGAGTGGAGTCCTTACCAG
CGTGCAGACGAAAACTATCAAAAAGTCTTTGAATCCAAAATGGAACGAAGAAATACTGTTCAGAGTCCTT
CCACAACAGCACCGGATTCTTTTCGAAGTGTTTGATGAAAATCGTTTGACAAGAGATGATTTCCTGGGTC
AAGTGGATGTCCCTCTCTATCCTTTACCGACTGAAAACCCAAGAATGGAGAGACCATATACATTTAAGGA
TTTTGTTCTTCATCCAAGAAGCCACAAATCAAGAGTTAAAGGTTATCTGAGATTAAAAATGACTTACTTA
CCTAAAAACGGCTCAGATGATGAAAATGCAGACCAGGCTGAGGAGTTAGAGCCTGGCTGGGTTGTTTTGG
ACCAACCAGATGCTGCCACCCATTTGCAGCATCCACCAGAACCTTCTCCCCTACCTCCAGGGTGGGAAGA
GAGGCAGGATGTCCTTGGAAGGACCTACTACGTAAACCATGAGTCTAGAACAACACAATGGAAAAGACCA
AGCCCTGAGGATGACCTCACAGATGATGAGAATGGTGACATTCAGCTGCAGGCGCAGCGAGCATTTACCA
CCAGGCGGCAGATATCGGAGGATGTGGATGGCCCTGACAACCACGAGTCCCCTGAGAATTGGGAGATTGT
ACGAGAAGATGAGAACACCATGTACAGTGGTCAGGCTGTTCAGTCGCCTCCGTCAGGTCACCCAGACGTG
CAGGTCCGCCTCGCAGAGGAGTTAGATACCAGACTCACCATGTATGGAAACCCGGCCACCAGCCAGCCAG
TCACCAGCTCAAACCATTCCAGCAGAGGAGGCAGCTCGCAAACCTGTATCTTTGAGGAACAGCCTACACT
TCCTGTGCTTTTGCCTACTTCATCTGGATTGCCACCAGGTTGGGAAGAAAAACAAGATGACAGAGGACGA
TCGTACTATGTAGACCACAACTCTAAGACAACCACATGGTCCAAGCCCACCATGCAGGATGATCCAAGAT
CGAAAATTCCTGCTCATCTGAGAGGAAAGACACCAGTCGACTCCAATGACCTGGGACCTTTACCTCCAGG
CTGGGAAGAAAGAACGCACACAGATGGAAGGGTCTTCTTCATAAATCACAATATAAAGAAGACACAGTGG
GAAGATCCTCGGATGCAGAATGTGGCCATAACTGGACCAGCAGTGCCCTACTCCAGAGATTACAAAAGAA
AGTATGAGTTTTTCCGAAGGAAGCTGAAGAAGCAGACTGACATTCCAAACAAATTTGAAATGAAACTTCG
CCGTGCAAACATTCTGGAGGATTCTTACCGGAGAATTATGGGTGTGAAGAGAGCTGACTTCCTCAAGGCC
CGACTCTGGATTGAGTTTGATGGTGAAAAGGGCCTTGACTATGGAGGAGTTGCCCGAGAATGGTTCTTCC
TCATCTCAAAGGAGATGTTTAATCCCTATTATGGCCTGTTTGAATATTCCGCTACGGACAATTACACTCT
GCAGATAAATCCAAACTCGGGCTTGTGTAATGAAGATCACCTCTCATACTTCAAGTTCATTGGCCGTGTG
GCTGGGATGGCGGTTTATCATGGCAAGCTGTTGGATGGGTTTTTCATCCGCCCCTTTTACAAGATGATGC
TTCAGAAACTGATAACACTGCACGACATGGAGTCCGTGGATAGTGAATATTACAGTTCTCTGCGATGGAT
TCTTGAAAATGACCCGACGGAGCTGGACCTGAGATTTATCATAGATGAAGAGCTTTTTGGACAGACACAT
CAACACGAGCTGAAAACTGGTGGATCGGAGGTTGTGGTCACCAACAAGAACAAAAAGGAGTATATCTACC
TTGTAATCCAGTGGCGATTTGTGAACAGAATCCAGAAGCAAATGGCTGCTTTTAAAGAGGGATTTTTTGA
ACTGATACCACAGGATCTGATCAAGATATTCGATGAAAATGAACTAGAGCTTCTCATGTGTGGTCTGGGA
GATGTGGACGTGAACGACTGGAGGGAACATACAAAATACAAAAATGGATACAGCTTAAACCACCAGGTCA
TCCACTGGTTCTGGAAGGCTGTTTTGATGATGGATTCAGAAAAAAGAATACGCTTACTTCAGTTTGTTAC
TGGCACATCCCGTGTGCCTATGAATGGATTTGCTGAACTCTATGGCTCAAATGGACCACAGTCCTTCACA
GTGGAACAGTGGGGCACCCCTGATAAGCTGCCCAGAGCACATACCTGCTTCAACCGCCTGGACCTGCCAC
CCTATGAATCCTTTGACGAACTCTGGGATAAACTTCAGATGGCGATTGAGAACACGCAGGGCTTTGATGG
GGTTGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RR214217 representing NM_012986
Red=Cloning site Green=Tags(s)

MSSDMAADDTEAPVLSEDEVWEFCLDKNEEGGGSPGSDVTDTCEPPCGCWELNPSSLEEEHVLFTADPYL
ELHNDDTRVVRVKVIAGIGLAKKDILGASDPYVRVTLYDPMSGVLTSVQTKTIKKSLNPKWNEEILFRVL
PQQHRILFEVFDENRLTRDDFLGQVDVPLYPLPTENPRMERPYTFKDFVLHPRSHKSRVKGYLRLKMTYL
PKNGSDDENADQAEELEPGWVVLDQPDAATHLQHPPEPSPLPPGWEERQDVLGRTYYVNHESRTTQWKRP
SPEDDLTDDENGDIQLQAQRAFTTRRQISEDVDGPDNHESPENWEIVREDENTMYSGQAVQSPPSGHPDV
QVRLAEELDTRLTMYGNPATSQPVTSSNHSSRGGSSQTCIFEEQPTLPVLLPTSSGLPPGWEEKQDDRGR
SYYVDHNSKTTTWSKPTMQDDPRSKIPAHLRGKTPVDSNDLGPLPPGWEERTHTDGRVFFINHNIKKTQW
EDPRMQNVAITGPAVPYSRDYKRKYEFFRRKLKKQTDIPNKFEMKLRRANILEDSYRRIMGVKRADFLKA
RLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRV
AGMAVYHGKLLDGFFIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILENDPTELDLRFIIDEELFGQTH
QHELKTGGSEVVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLG
DVDVNDWREHTKYKNGYSLNHQVIHWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFT
VEQWGTPDKLPRAHTCFNRLDLPPYESFDELWDKLQMAIENTQGFDGVD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_012986
ORF Size 2667 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012986.1, NP_037118.1
RefSeq Size 5373 bp
RefSeq ORF 2670 bp
Locus ID 25489
UniProt ID Q62940
MW 102.8 kDa
Gene Summary may regulate function of the amiloride-sensitive epithelial sodium channel ENaC [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
RN214217 Nedd4 (untagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated gene 4 (Nedd4), (10 ug)
    • 10 ug

CNY 7,168.00

RR214217L3 Lenti ORF clone of Nedd4 (Myc-DDK-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated gene 4 (Nedd4), (10 ug)
    • 10 ug

CNY 13,210.00

RR214217L4 Lenti ORF clone of Nedd4 (mGFP-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated gene 4 (Nedd4), (10 ug)
    • 10 ug

CNY 14,350.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

其它Nedd4产品

Expression Plasmids
Lentiviral Particles
CRISPR
RNAi
Proteins
Antibodies
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RN214217

Nedd4 (untagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated gene 4 (Nedd4), (10 ug)

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RR214217L3

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RR214217L3V

Lenti ORF particles, Nedd4 (Myc-DDK-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated gene 4 (Nedd4), 200ul, >10^7 TU/mL

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RR214217L4V

Lenti ORF particles, Nedd4 (GFP-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated gene 4 (Nedd4), 200ul, >10^7 TU/mL

    • 200 ul

CNY 15,485.00 暂停销售

RR214217L4

Lenti ORF clone of Nedd4 (mGFP-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated gene 4 (Nedd4), (10 ug)

    • 10 ug

CNY 14,350.00

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