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Lrrc8c (NM_001037179) Rat Tagged ORF Clone
CAT＃: RR214224
- TrueORF®
Lrrc8c (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 8 family, member C (Lrrc8c), (10 ug)
"NM_001037179" in other vectors (3)
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CNY 10,260.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RGD1306585 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214224 representing NM_001037179
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCCAGTGACCGAGTTCCGGCAGTTCTCCGAGCAGCAGCCTGCCTTCCGGGTGCTGAAGCCGTGGT GGGATGTGTTCACGGACTACCTCTCGGTGGCCATGCTGATGATCGGGGTGTTTGGATGTACTTTACAAGT CATGCAAGACAAGATCATCTGCCTCCCAAAAAGAGTGCAGCCTGCTCAGAACCACTCTTCCCTCTCCAAC GTCTCCCAGACTGTGATCAATACCACCCCGCTGCCCCCACCTAAACCCTCTCCGACCAACCCGGCGACCG TGGAGATGAAGGGACTGAAGACAGACCTGGACCTCCAGCAGTACAGTTTCATCAACCAGATGTGCTACGA GCGAGCCCTCCACTGGTATGCCAAGTACTTCCCGTACCTTGTGCTCATCCACACCCTGGTCTTCATGCTC TGCAGCAACTTCTGGTTCAAGTTCCCTGGATCTAGTTCCAAAATAGAACATTTCATCTCCATCCTGGGGA AGTGCTTCGACTCCCCGTGGACCACTCGGGCTCTCTCCGAGGTGTCTGGCGAGGACTCCGAAGAGAAGGA CAATAGGAAGAACAACATGAACAGGTCCAACACCATCCAGTCCGGTCCGGAAGGCAGCCTGGTCAAGTCC CAGTCTCTCAAGTCAATTCCCGAGAAGTTCGTGGTTGACAAATCCACTGCGGGGGCTCTGGACAAGAAGG AAGGTGAACAGGCCAAGGCCTTGTTCGAGAAGGTTAAGAAGTTCAGACTGCACGTGGAGGAAGGTGACAT CCTGTATGCCATGTATGTGCGGCAGACTGTGCTTAAGGTCATCAAGTTCCTGATCATCATCGCCTACAAC AGTGCTCTGGTTTCCAAAGTCCAGTTCACCGTGGACTGCAATGTGGACATCCAGGACATGACGGGCTATA AGAACTTTTCCTGCAATCACACCATGGCTCATTTGTTCTCCAAACTCTCCTTTTGCTACCTGTGCTTTGT AAGCATCTACGGCCTGACGTGCCTTTATACCTTGTACTGGCTGTTCTACCGTTCTCTGAGGGAGTACTCT TTTGAGTATGTCCGGCAGGAGACTGGAATCGATGACATTCCGGACGTGAAAAATGACTTTGCCTTTATGC TCCATATGATAGACCAGTATGACCCTCTCTATTCCAAGAGGTTTGCGGTGTTCCTCTCTGAGGTCAGCGA GAACAAGTTAAAGCAGCTCAACTTAAATAACGAGTGGACCCCCGACAAGCTGCGGCAGAAGCTGCAGACG AATGCCCACAACCGCCTGGAGCTGCCTCTCATCATGCTGTCTGGCCTCCCAGACACCGTGTTCGAGATCA CGGAGTTACAGTCCCTGAAGCTGGAGATCATTAAGAACGTCATGATACCCGCCACCATCGCCCAGCTAGA CAACCTTCAGGAGCTCTCCCTCCACCAGTGCTCCGTCAAGATCCACAGTGCGGCACTCTCCTTCCTGAAG GAGAATCTCAAGGTCTTGAGCGTCAAGTTCGATGACATGAGGGAGCTGCCCCCCTGGATGTACGGCCTCC GGAATCTGGAAGAGCTCTATCTGGTTGGCTCTCTGAGTCACGACATCTCCAAAAACGTCACCCTGGAGTC CCTGCGGGACCTCAAAAGCCTTAAAATCCTTTCCATCAAGAGCAACGTCTCCAAGATCCCTCAGGCCGTG GTGGATGTGTCCAGCCACCTCCAGAAGATGTGCATTCACAACGACGGCACCAAGCTGGTAATGCTCAACA ACCTGAAGAAGATGACCAACCTGACCGAGCTGGAACTGGTCCACTGCGACCTGGAACGCATTCCCCACGC CGTGTTCAGCCTGCTCAGTCTCCAGGAGCTGGACCTGAAGGAGAACAACCTGAAGTCCATAGAGGAGATC GTGAGTTTCCAGCACTTGAGAAAGCTAACCGTGCTCAAACTGTGGTATAACAGCATCGCTTACATCCCAG AGCACATCAAGAAACTGACCAGCCTGGAGCGACTGTTTTTCAGCCACAATAAGGTAGAAGTGCTTCCCTC CCACCTCTTCCTGTGCAACAAAATCAGATACCTGGACCTGTCCTATAACGACATTCGCTTCATCCCGCCC GAAATCGGGGTTCTGCAAAGTTTACAGTATTTCTCCATCACCTGTAACAAAGTGGAGAGCCTCCCGGATG AACTCTACTTCTGCAAGAAACTTAAAACATTGAAGATCGGGAAAAACAGCCTCTCTGTACTTTCACCAAA AATTGGAAACTTACTATTTCTTTCCTACTTAGACATCAAAGGCAATCACTTTGAAGTCCTCCCTCCCGAG CTGGGAGACTGCCGGGCTCTGAAACGAGCCGGGCTGGTTGTGGAAGACGCTCTGTTTGAGACTCTGCCCT CAGATGTCCGGGAGCAAATGAAAGCAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214224 representing NM_001037179
Red=Cloning site Green=Tags(s) MIPVTEFRQFSEQQPAFRVLKPWWDVFTDYLSVAMLMIGVFGCTLQVMQDKIICLPKRVQPAQNHSSLSN VSQTVINTTPLPPPKPSPTNPATVEMKGLKTDLDLQQYSFINQMCYERALHWYAKYFPYLVLIHTLVFML CSNFWFKFPGSSSKIEHFISILGKCFDSPWTTRALSEVSGEDSEEKDNRKNNMNRSNTIQSGPEGSLVKS QSLKSIPEKFVVDKSTAGALDKKEGEQAKALFEKVKKFRLHVEEGDILYAMYVRQTVLKVIKFLIIIAYN SALVSKVQFTVDCNVDIQDMTGYKNFSCNHTMAHLFSKLSFCYLCFVSIYGLTCLYTLYWLFYRSLREYS FEYVRQETGIDDIPDVKNDFAFMLHMIDQYDPLYSKRFAVFLSEVSENKLKQLNLNNEWTPDKLRQKLQT NAHNRLELPLIMLSGLPDTVFEITELQSLKLEIIKNVMIPATIAQLDNLQELSLHQCSVKIHSAALSFLK ENLKVLSVKFDDMRELPPWMYGLRNLEELYLVGSLSHDISKNVTLESLRDLKSLKILSIKSNVSKIPQAV VDVSSHLQKMCIHNDGTKLVMLNNLKKMTNLTELELVHCDLERIPHAVFSLLSLQELDLKENNLKSIEEI VSFQHLRKLTVLKLWYNSIAYIPEHIKKLTSLERLFFSHNKVEVLPSHLFLCNKIRYLDLSYNDIRFIPP EIGVLQSLQYFSITCNKVESLPDELYFCKKLKTLKIGKNSLSVLSPKIGNLLFLSYLDIKGNHFEVLPPE LGDCRALKRAGLVVEDALFETLPSDVREQMKAD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001037179 |
| ORF Size | 2409 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001037179.1, NP_001032256.1 |
| RefSeq Size | 2883 bp |
| RefSeq ORF | 2412 bp |
| Locus ID | 289443 |
| UniProt ID | Q498T9 |
| MW | 92.5 kDa |
| Gene Summary | Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:28833202). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (By similarity). Plays a redundant role in the efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:28833202).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214224 | Lrrc8c (untagged ORF) - Rat leucine rich repeat containing 8 family, member C (Lrrc8c), (10 ug) |
CNY 10,260.00 |
|
| RR214224L3 | Lenti ORF clone of Lrrc8c (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 8 family, member C (Lrrc8c), (10 ug) |
CNY 12,160.00 |
|
| RR214224L4 | Lenti ORF clone of Lrrc8c (mGFP-tagged ORF) - Rat leucine rich repeat containing 8 family, member C (Lrrc8c), (10 ug) |
CNY 13,300.00 |
