Plcg1 (NM_013187) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PPLCA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214485 representing NM_013187 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGTCGGGACCCCCTGCGCCAACGGCTGCGGGCCCAGCGCGCCCTCCGAAGCGGAGGTGCTGC ACCTCTGCCGCAGCCTCGAGGTGGGCACCGTCATGACTTTGTTCTACTCCAAGAAGTCGCAGCGGCCAGA ACGGAAGACCTTCCAGGTCAAGTTGGAGACGCGCCAGATCACATGGAGCCGCGGTGCGGACAAAATCGAG GGGTCCATCGATATCCGAGAAATCAAGGAGATCCGCCCAGGGAAGACTTCCCGGGACTTTGACCGCTACC AAGAAGACCCTGCCTTCCGGCCAGATCAGTCACACTGTTTTGTCATCCTCTATGGAATGGAATTCCGCCT GAAGACCCTGAGCCTGCAAGCCACATCTGAGGATGAAGTGAACATGTGGATCAAGGGCTTAACTTGGCTC ATGGAAGATACGCTGCAGGCGGCCACACCCCTGCAAATTGAGAGATGGCTCCGGAAGCAGTTCTACTCAG TGGATCGTAACCGAGAGGATCGTATATCAGCCAAGGACCTGAAGAACATGCTGTCACAGGTCAACTACCG GGTCCCCAACATGCGCTTCCTCCGAGAGCGGCTGACGGACTTTGAACAGCGCAGCGGGGACATCACCTAT GGGCAGTTTGCTCAGCTTTACCGCAGCCTCATGTACAGCGCCCAGAAGACGATGGACCTTCCGTTCTTGG AAACCAACACTTTGAGGACTGGAGAGCGGCCAGAGCTTTGCCAGGTGTCCCTTTCTGAGTTCCAGCAGTT CCTTCTTGAGTACCAGGGGGAGCTGTGGGCTGTCGACCGGCTTCAGGTGCAGGAATTTATGCTCAGCTTC CTTCGAGACCCCTTGCGAGAGATTGAGGAGCCATACTTCTTCTTGGATGAGCTTGTCACCTTTCTGTTCT CCAAAGAGAACAGTGTGTGGAACTCACAGCTGGATGCCGTGTGCCCAGAAACCATGAACAACCCACTGTC TCACTATTGGATCTCTTCCTCGCATAATACGTATCTGACTGGGGACCAGTTCTCCAGCGAGTCCTCCCTG GAAGCCTACGCTCGCTGCCTGAGGATGGGCTGTCGCTGCATCGAGTTGGACTGCTGGGATGGGCCAGATG GGATGCCAGTCATTTACCATGGGCACACCCTCACCACCAAGATTAAGTTCTCAGATGTCCTGCACACCAT CAAGGAGCACGCGTTCGTAGCCTCAGAGTACCCTGTCATCCTGTCCATCGAGGACCACTGCAGCATTGCC CAGCAGAGGAACATGGCCCAGCACTTCAGGAAGGTGCTCGGTGACACGCTCCTCACCAAGCCCGTGGACA TTGCCGCTGATGGGCTCCCTTCTCCCAACCAGCTCAAGAGGAAGATCCTGATTAAGCATAAGAAGCTGGC TGAGGGCAGTGCCTATGAGGAGGTGCCTACCTCTGTGATGTACTCTGAGAATGACATCAGTAACTCCATC AAGAATGGTATCCTCTACTTGGAGGACCCCGTGAATCATGAGTGGTACCCCCACTACTTTGTTCTGACTA GCAGCAAGATCTACTACTCTGAGGAGACCAGCAGTGACCAGGGAAATGAGGATGAAGAGGAGCCGAAGGA GGCCAGTGGCAGCACAGAGCTGCACTCGAGCGAGAAGTGGTTCCACGGGAAGCTCGGGGCTGGGCGCGAC GGGCGGCACATTGCTGAGCGCCTGCTCACCGAGTACTGCATAGAGACTGGGGCTCCCGATGGCTCCTTCC TAGTGCGAGAAAGTGAGACCTTCGTGGGGGACTACACGCTGTCTTTTTGGCGGAATGGGAAAGTCCAGCA CTGCCGTATCCACTCCCGGCAGGATGCTGGGACTCCTAAGTTCTTCTTGACAGATAACCTTGTCTTTGAC TCTCTCTATGACCTCATCACACATTATCAGCAAGTGCCCCTGCGCTGCAATGAGTTTGAGATGCGCCTTT CAGAGCCTGTTCCACAGACGAATGCCCATGAGAGCAAAGAGTGGTACCACGCAAGCCTGACTAGAGCTCA GGCTGAACACATGCTGATGCGAGTACCCCGTGATGGGGCCTTCCTGGTGCGGAAGCGCAACGAGCCCAAC TCCTATGCCATCTCTTTCCGGGCTGAGGGAAAGATCAAGCACTGCCGAGTACAGCAGGAAGGCCAGACTG TGATGCTGGGGAACTCTGAGTTTGACAGCCTGGTCGACCTCATCAGCTACTATGAGAAGCATCCCCTGTA CCGCAAAATGAAACTGCGCTACCCCATCAACGAGGAGGCGCTGGAGAAGATTGGGACAGCTGAACCCGAT TATGGGGCACTGTATGAGGGCCGCAACCCTGGTTTCTATGTGGAGGCCAACCCTATGCCAACTTTCAAGT GTGCAGTAAAAGCTCTCTTCGACTACAAGGCCCAGAGAGAGGATGAGCTGACTTTTACCAAGAGCGCCAT CATCCAGAATGTGGAAAAGCAAGATGGTGGCTGGTGGCGTGGGGACTATGGTGGGAAGAAGCAGCTGTGG TTCCCCTCAAACTATGTGGAAGAGATGATCAATCCAGCAATCCTAGAGCCGGAGAGGGAGCATCTGGATG AGAACAGCCCACTGGGGGACTTGCTGCGAGGGGTCTTAGATGTGCCAGCCTGCCAGATCGCCATTCGTCC TGAGGGCAAAAACAACCGGCTCTTCGTCTTCTCCATCAGCATGCCGTCAGTGGCTCAGTGGTCCCTAGAC GTTGCCGCTGACTCACAGGAGGAGTTGCAGGACTGGGTGAAAAAGATCCGTGAAGTTGCCCAGACTGCAG ATGCCAGGCTTACTGAGGGGAAGATGATGGAGAGGAGGAAGAAGATCGCCTTGGAGCTCTCCGAGCTCGT GGTCTACTGCCGGCCTGTTCCCTTTGACGAAGAGAAGATTGGCACAGAACGCGCTTGTTACCGGGACATG TCCTCCTTTCCGGAAACCAAGGCTGAGAAGTATGTGAACAAGGCCAAAGGCAAGAAGTTCCTCCAGTACA ACCGGCTGCAGCTCTCTCGCATCTACCCTAAGGGTCAGAGGCTGGACTCCTCCAATTATGACCCTCTGCC CATGTGGATCTGTGGCAGCCAGCTTGTAGCTCTCAATTTTCAGACCCCAGACAAGCCTATGCAGATGAAC CAGGCCCTCTTCATGGCTGGTGGACACTGTGGCTATGTGCTGCAGCCAAGCACCATGAGAGATGAAGCCT TTGACCCCTTTGATAAGAGCAGTCTCCGAGGTCTGGAGCCCTGTGTCATTTGCATTGAGGTGCTGGGGGC CAGGCATCTGCCGAAGAATGGCCGGGGTATTGTGTGTCCTTTCGTGGAGATTGAAGTGGCTGGGGCAGAG TACGACAGCACCAAGCAGAAGACAGAGTTTGTAGTGGACAATGGACTGAACCCTGTGTGGCCTGCAAAGC CCTTCCACTTCCAGATCAGTAACCCAGAGTTTGCCTTTCTGCGCTTTGTGGTGTATGAGGAAGACATGTT TAGTGACCAGAACTTCTTGGCTCAGGCTACTTTCCCAGTAAAAGGCCTGAAGACAGGATACAGAGCAGTG CCTTTGAAGAACAACTACAGTGAAGACCTGGAGTTGGCCTCCCTGCTCATCAAGATTGACATTTTCCCTG CTAAGGAGAATGGTGACCTCAGCCCTTTCAGTGGTACATCCCTAAGGGAACGGGCCTCAGATGCCTCCAG CCAGCTGTTCCATGTCCGGGCCCGGGAAGGGTCCTTTGAAGCCAGATACCAGCAGCCATTTGAAGACTTC CGCATCTCGCAGGAGCATCTCGCAGACCATTTTGACAGTCGGGAACGAAGGGCCCCAAGAAGGACTCGGG TCAATGGAGACAACCGCCTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214485 representing NM_013187 Red=Cloning site Green=Tags(s) MAGVGTPCANGCGPSAPSEAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIE GSIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWL MEDTLQAATPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDFEQRSGDITY GQFAQLYRSLMYSAQKTMDLPFLETNTLRTGERPELCQVSLSEFQQFLLEYQGELWAVDRLQVQEFMLSF LRDPLREIEEPYFFLDELVTFLFSKENSVWNSQLDAVCPETMNNPLSHYWISSSHNTYLTGDQFSSESSL EAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIA QQRNMAQHFRKVLGDTLLTKPVDIAADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSVMYSENDISNSI KNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEASGSTELHSSEKWFHGKLGAGRD GRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFD SLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPN SYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPD YGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFTKSAIIQNVEKQDGGWWRGDYGGKKQLW FPSNYVEEMINPAILEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMPSVAQWSLD VAADSQEELQDWVKKIREVAQTADARLTEGKMMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDM SSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMN QALFMAGGHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCVICIEVLGARHLPKNGRGIVCPFVEIEVAGAE YDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAV PLKNNYSEDLELASLLIKIDIFPAKENGDLSPFSGTSLRERASDASSQLFHVRAREGSFEARYQQPFEDF RISQEHLADHFDSRERRAPRRTRVNGDNRL SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene
ACCN	NM_013187
ORF Size	3870 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013187.1, NP_037319.1
RefSeq Size	5106 bp
RefSeq ORF	3873 bp
Locus ID	25738
UniProt ID	P10686
MW	148.5 kDa
Gene Summary	mediates intracellular agonist-induced Ca2+ entry (ACE); serves as a guanine nucleotide exchange factor for the nuclear GTPase PIKE [RGD, Feb 2006]

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