Sfrp1 (NM_001276712) Rat Tagged ORF Clone
CAT#: RR216324
- TrueORF®
Sfrp1 (myc-DDK-tagged) - Rat secreted frizzled-related protein 1 (Sfrp1)
"NM_001276712" in other vectors (1)
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CNY 3,990.00
Cited in 3 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | sFRP-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR216324 representing NM_001276712
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGTCGGGCGCAACGCGCGGGGTCGCGGCGGGGCCGCCTCCGGCGTGCTGCTGGCGTTGGCCGCCG CGCTGCTGGCCGCGGGTTCGGCCAGCGAGTACGACTACGTGAGCTTCCAGTCGGATATCGGCTCCTATCA GAGCGGGCGCTTCTACACCAAGCCCCCGCAGTGCGTGGACATCCCGGTGGACCTGCGGCTGTGCCACAAC GTGGGCTACAAGAAGATGGTGCTGCCCAACCTGCTGGAGCACGAGACCATGGCAGAGGTGAAGCAGCAGG CCAGCAGCTGGGTGCCGCTGCTCAACAAGAACTGCCACATGGGCACCCAGGTCTTCCTCTGCTCGCTCTT CGCGCCCGTCTGTCTGGACCGGCCTATCTACCCGTGTCGCTGGCTCTGCGAGGCTGTTCGCGACTCGTGC GAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTCAAATGTGACAAGTTCCCCGAGGGCG ACGTCTGCATCGCCATGACCCCGCCCAATGCCACCGAAGCCTCGAAGCCCCAAGGTACAACAGTGTGTCC TCCGTGTGACAACGAGTTGAAATCGGAGGCCATCATCGAACATCTCTGTGCAAGCGAGTTTGCGCTGAGA ATGAAAATCAAAGAAGTAAAAAAGGAACGGGTGCAAGAGATTGTCCCCAAGAAGAAGAAACCCTTGAAGC TGGGGCCCATCAAGAAGAAGGAGCTGAAGCGGCTCGTGCTTTTCCTAAAGAATGGCGCCGACTGTCCCTG CCACCAGCTGGACAACCTCAGCCACAACTTCCTCATCATGGGGCGCAAGGTGAAGAGCCAGTACTTGCTC ACAGCCATTCACAAGTGGGACAAGAAAAACAAGGAGTTCAAAAACTTCATGAAGAAAATGAAAAACCACG AGTGTCCCACCTTCCAGTCTGTTTTTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR216324 representing NM_001276712
Red=Cloning site Green=Tags(s) MGVGRNARGRGGAASGVLLALAAALLAAGSASEYDYVSFQSDIGSYQSGRFYTKPPQCVDIPVDLRLCHN VGYKKMVLPNLLEHETMAEVKQQASSWVPLLNKNCHMGTQVFLCSLFAPVCLDRPIYPCRWLCEAVRDSC EPVMQFFGFYWPEMLKCDKFPEGDVCIAMTPPNATEASKPQGTTVCPPCDNELKSEAIIEHLCASEFALR MKIKEVKKERVQEIVPKKKKPLKLGPIKKKELKRLVLFLKNGADCPCHQLDNLSHNFLIMGRKVKSQYLL TAIHKWDKKNKEFKNFMKKMKNHECPTFQSVFK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001276712 |
ORF Size | 939 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001276712.1, NP_001263641.1 |
RefSeq Size | 3892 bp |
RefSeq ORF | 942 bp |
Locus ID | 84402 |
MW | 35.4 kDa |
Gene Summary | a secreted protein involved in binding Wnt and antagonizing Wnt signaling [RGD, Feb 2006] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
---|
Functional analyses and prognostic significance of SFRP1 expression in bladder cancer.
,null,
Journal of cancer research and clinical oncology
,PubMed ID 25732201
[Sfrp1]
|
BMP and TGF-? pathway mediators are critical upstream regulators of Wnt signaling during midbrain dopamine differentiation in human pluripotent stem cells
,null,
Developmental biology
,PubMed ID 23352789
[Sfrp1]
|
Keloid fibroblasts are more sensitive to Wnt3a treatment in terms of elevated cellular growth and fibronectin expression.
,null,
Journal of dermatological science
,PubMed ID 22005028
[Sfrp1]
|
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN216324 | Sfrp1 (untagged) - Rat secreted frizzled-related protein 1 (Sfrp1) |
CNY 3,040.00 |