Pex5 (NM_001170584) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PTS1-BP; PTS1R
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216987 representing NM_001170584 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAATGCGGGAGCTGGTGGAGAGCGAATGTGGGGGTGCCAACCCGCTGATGAAGCTGGCCACCCACT TCACCCAGGACAAGGCCCTTCGGCAGGAGGGACTGAGGCCTGGCCCCTGGCCTCCAGGAGCCTCAGCGGC GGAAACGGTTTCCAAGCCTTTGGGAGTAGGCACTGAAGATGAGTTGGTGGCAGAATTCCTGCAGGACCAG AATGCAACTCTTGTATCCCGAGCCCCTCAGACCTTCAAGATGGATGACCTCTTGGCGGAGATGCAGGAGA TTGAACAGTCAAACTTTCGCCAGGCTCCTCAGAGAGCCCCTGGTGTGGCAGACTTGGCTTTGTCTGAGAA TTGGGCCCAGGAGTTTCTTGCCGCTGGAGATGCTGTGGATGTAGCTCAGGATTATAACGAGACCGACTGG TCCCAAGAATTCATCGCCGAAGTCACGGACCCTCTGTCTGTGTCCCCAGCCCGATGGGCTGAGGAGTATC TGGAACAGTCTGAGGAGAAGCTGTGGTTGGGAGATCCAGAAGGAACCTCTACCACTGACCGATGGTACGA TGAATACCATCCTGAGGAGGATCTGCAGCACACAGCCAGTGACTTTGTGTCCAAGGTGGACGACCCCAAA TTGGCTAACTCTGAGGGCACATCGGAGGCCTGGGTTGATCAGTTCACAAGACCAGGAAACAAACTGGCTG CTCTTCAGGTGGAATTTGAACGAGCCAAGTCAGCTATAGAGTCAGACGTCGATTTCTGGGACAAGTTGCA GGCAGAGTTGGAGGAGATGGCAAAACGGGATGCTGAGGCGCACCCCTGGCTTTCTGACTATGACGACCTC ACGTCTGCTTCCTATGACAAGGGCTACCAGTTTGAGGAGGAGAATCCCTTGCGTGACCACCCTCAGCCTT TTGAAGAAGGGCTGCGTCGACTTGAGGAGGGAGACCTGCCCAATGCTGTGCTGCTCTTCGAGGCTGCGGT GCAGCAGGATCCTAAGCACATGGAGGCCTGGCAGTACCTGGGCACCACTCAGGCTGAGAACGAGCAAGAG CTCTTGGCCATCAGTGCCCTGCGCAGGTGTCTGGAGCTAAAGCCAGATAACCGGACAGCACTGATGGCGC TGGCTGTAAGCTTCACCAATGAGTCCCTGCAGCGGCAAGCCTGTGAGACGCTGCGGGACTGGCTGCGCTA CTCACCGGCCTACGCTCACCTGGTGGCTCCTGGTGAAGAAGGGGCTAGTGGGGCAGGACTGGGTCCCAGC AAGCGCGTCTTGGGATCTCTGTTGTCTGACTCCCTGTTTCTTGAAGTAAAAGAACTCTTCCTGGCAGCTG TGCGTCTGGACCCTACATCCATTGACCCTGACGTGCAGTGTGGCCTGGGAGTCCTCTTCAACCTGAGTGG GGAGTACGACAAGGCTGTGGACTGTTTCACGGCTGCCCTCAGTGTCCGCCCCAATGACTATTTGCTGTGG AACAAGCTTGGGGCCACCCTGGCCAACGGGAACCAGAGTGAAGAAGCAGTGGCTGCTTACCGCAGAGCCC TTGAACTACAGCCTGGGTACATCCGCTCCCGCTACAACCTAGGCATCAGCTGCATCAACCTTGGGGCTCA CCGTGAGGCCGTGGAACACTTTCTGGAGGCCCTGAACATGCAGAGGAAGAGCCGGGGCCCTCGGGGTGAG GGGGGCGCCATGTCTGAGAACATCTGGAGTACTCTGCGGTTGGCATTGTCCATGCTAGGCCAGAGTGATG CCTATGGGGCAGCCGATGCTCGGGACCTGCCTGCCCTTCTAACTATGTTTGGCCTGCCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216987 representing NM_001170584 Red=Cloning site Green=Tags(s) MAMRELVESECGGANPLMKLATHFTQDKALRQEGLRPGPWPPGASAAETVSKPLGVGTEDELVAEFLQDQ NATLVSRAPQTFKMDDLLAEMQEIEQSNFRQAPQRAPGVADLALSENWAQEFLAAGDAVDVAQDYNETDW SQEFIAEVTDPLSVSPARWAEEYLEQSEEKLWLGDPEGTSTTDRWYDEYHPEEDLQHTASDFVSKVDDPK LANSEGTSEAWVDQFTRPGNKLAALQVEFERAKSAIESDVDFWDKLQAELEEMAKRDAEAHPWLSDYDDL TSASYDKGYQFEEENPLRDHPQPFEEGLRRLEEGDLPNAVLLFEAAVQQDPKHMEAWQYLGTTQAENEQE LLAISALRRCLELKPDNRTALMALAVSFTNESLQRQACETLRDWLRYSPAYAHLVAPGEEGASGAGLGPS KRVLGSLLSDSLFLEVKELFLAAVRLDPTSIDPDVQCGLGVLFNLSGEYDKAVDCFTAALSVRPNDYLLW NKLGATLANGNQSEEAVAAYRRALELQPGYIRSRYNLGISCINLGAHREAVEHFLEALNMQRKSRGPRGE GGAMSENIWSTLRLALSMLGQSDAYGAADARDLPALLTMFGLPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001170584
ORF Size	1812 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170584.1, NP_001164055.1
RefSeq Size	3197 bp
RefSeq ORF	1815 bp
Locus ID	312703
UniProt ID	Q2M2R8
MW	67 kDa
Gene Summary	Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import.[UniProtKB/Swiss-Prot Function]

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