Dync1i2 (NM_001270625) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Dnci2; Dncic2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216999 representing NM_001270625 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGACAAAAGTGAATTAAAAGCTGAGTTGGAACGTAAGAAGCAACGGTTGGCCCAAATCAGAGAAG AAAAGAAGAGGAAAGAAGAGGAAAGGAAAAAAAAAGAAACTGATCAGAAGAAGGAAGCTGCAGTCTCTGT GCAGGAAGAGTCTGACCTTGAAAAGAAGAGAAGAGAAGCCGAAGCCTTGCTTCAGAGCATGGGGCTAACT ACAGACTCCCCAATTGTCCCTCCTCCCATGTCTCCATCCTCCAAGTCGGTGAGCACGCCAAGTGAAGCTG GAAGCCAGGACTCTGGAGATGGCGCCGTGGGATCTAGACGAGGACCTATTAAACTTGGAATGGCCAAAAT TACTCAAGTCGACTTTCCCCCTCGAGAAATCGTCACATATACAAAGGAAACTCAGACCCCGGTTACAGCT CAACCCAAAGAAGATGAGGAGGAGGAAGATGACGTAGCAGCTCCTAAGCCTCCTGTTGAACCTGAAGAAG AGAAAATTTTAAAAAAAGACGAAGAGAATGATAGCAAAGCTCCCCCTCATGAACTAACTGAAGAAGAAAA GCAACAAATTTTACACTCAGAAGAATTTTTAAGTTTCTTTGACCATTCTACAAGAATTGTAGAAAGAGCC CTCTCTGAGCAGATTAACATCTTCTTTGACTACAGTGGGAGAGATTTGGAAGACAAAGAAGGGGAGATTC AAGCAGGTGCTAAGCTGTCATTAAATCGACAGTTTTTTGATGAGCGTTGGTCAAAGCATCGAGTTGTTAG TTGCTTGGATTGGTCATCCCAGTATCCAGAATTACTTGTGGCTTCCTATAATAACAATGAAGAGGCTCCT CATGAGCCTGATGGTGTGGCCCTCGTATGGAATATGAAGTACAAGAAAACTACCCCAGAGTACGTGTTCC ACTGCCAGTCAGCTGTAATGTCTGCTACATTTGCAAAATTTCATCCAAATCTTGTTGTTGGAGGTACATA CTCAGGCCAAATTGTGCTTTGGGATAACCGTAGCAATAAAAGAACCCCCGTCCAGAGAACACCACTGTCA GCTGCTGCACACACACACCCTGTCTACTGTGTAAATGTTGTTGGAACACAAAATGCTCATAATCTGATTA GCATCTCTACTGATGGAAAAATTTGCTCCTGGAGCTTAGACATGCTTTCCCATCCACAGGATAGCATGGA GTTGGTTCATAAACAGTCAAAGGCAGTAGCTGTAACATCTATGTCTTTCCCTGTTGGAGACGTCAACAAC TTTGTTGTGGGCAGTGAAGAAGGTTCTGTGTATACAGCATGCCGCCATGGCAGCAAAGCCGGAATCAGTG AGATGTTTGAGGGACACCAGGGACCCATCACGGGAATCCACTGTCACGCAGCTGTTGGCGCAGTAGACTT CTCACATCTGTTCGTCACTTCGTCTTTTGACTGGACAGTAAAACTTTGGACAACTAAGAATAACAAGCCT TTGTACTCATTTGAAGATAATTCAGACTATGTCTATGATGTTATGTGGTCACCCACCCACCCAGCCCTGT TTGCCTGTGTGGATGGCATGGGGAGACTGGATCTATGGAATCTCAACAATGACACAGAGGTGCCGACTGC CAGCATTTCTGTGGAGGGCAACCCTGCCCTTAACCGTGTGAGATGGACCCACTCGGGAAGAGAGATCGCC GTGGGTGATTCTGAAGGACAGATTGTCATATATGATGTGGGCGAGCAGATCGCTGTTCCCCGAAATGATG AATGGGCAAGATTTGGCCGAACACTCGCAGAGATTAACGCAAGCCGAGCTGATGCAGAGGAGGAAGCGGC TACCCGGATTCCTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216999 representing NM_001270625 Red=Cloning site Green=Tags(s) MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAAVSVQEESDLEKKRREAEALLQSMGLT TDSPIVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRRGPIKLGMAKITQVDFPPREIVTYTKETQTPVTA QPKEDEEEEDDVAAPKPPVEPEEEKILKKDEENDSKAPPHELTEEEKQQILHSEEFLSFFDHSTRIVERA LSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSCLDWSSQYPELLVASYNNNEEAP HEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLS AAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNN FVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKP LYSFEDNSDYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALNRVRWTHSGREIA VGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINASRADAEEEAATRIPA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270625
ORF Size	1836 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270625.1, NP_001257554.1
RefSeq Size	2743 bp
RefSeq ORF	1839 bp
Locus ID	116659
MW	68.4 kDa
Gene Summary	intermediate chain of cytoplasmic dynein, a retrograde microtubule motor thought to be involved in organelle transport [RGD, Feb 2006]

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