Ddx3 (NM_001167665) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Ddx3y
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217050 representing NM_001167665 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGAAAATACGGTTGGCCTTGACCAGCAATTTGCTGGTCTTGACATGAACTCAGATAATCAGA ATGGAGGAGTTAGTACAGAAAGCAAAGGGCGCTATATACCTCCTCACTTAAGGAACAGAGAAACATCTAA AGGTGTCTGTGATAAGGACAATTCAGGGTGGAGTAAAGATAAAGATGCCTACAGCAGTTTTGGATCTCGG GATTCCAGAGGAAAACCCAATTATTTCAGTGATCGTGGAAGTGGATCCAGAGGAAGGTTTGATGATCCTG GTCGAAGTGACTATGATTGTATTGCCAGTCGTGACCGAAGTGGATTTGGCAAATTTGAACGAAGTGGTTA TAGTCGTTGGGGTGACAAATCAGATGAAGATGATTGGTCAAAACCACTTCCACCAAGTGAACGCTTAGAG CAAGAACTTTTCTCTGGAGGTAATGCAGGGATTAACTTTGAGAAATATGATGATATACCAGTAGAGGCAA CTGGAAATAATTGTCCTCCTCACATTGAAAATTTCAGTGATGTTGAGATGGGAGAAATTATCATGGGAAA CATTGAACTTACTCGTTACACTCGTCCTACTCCAGTTCAGAAACATGCTATTCCAATTATTAAAGAAAAA AGGGACTTAATGGCTTGTGCTCAAACAGGGTCTGGATGTACTGCTGCATTTCTACTACCCATTCTCAGTC AGATTTACACAGATGGTCCAGGAGAGGCTTTGAAGGCTATGAAGGAAAACGGAAGATATGGTCGCCGTAA ACAATACCCAATCTCCTTGGTGTTAGCTCCAACAAGAGAATTGGCTGTGCAGATCTATGAGGAAGCCAGA AAATTTTCGTACCGGTCTAGAGTCCGACCTTGTGTAGTGTATGGTGGTGCTGATACTGTTCAACAGATTC GGGACTTAGAACGAGGATGCCACTTGTTAGTTGCCACACCAGGACGTCTAGTGGACATGATGGAAAGAGG AAAGATTGGACTAGATTTCTGCAAATACTTAGTGTTGGATGAAGCTGACAGGATGCTGGATATGGGATTT GAACCTCAGATACGTCGTATAGTTGAACAGGACACTATGCCACCAAAGGGAGTTCGTCACACCATGATGT TTAGTGCTACTTTTCCTAAGGAGATACAGATGCTTGCTCGTGACTTTTTAGATGAATACATCTTTCTGGC TGTAGGCAGAGTAGGCTCTACTTCTGAGAACATCACACAGAAAGTAGTATGGGTTGAAGAGTTAGACAAA CGGTCATTTTTACTTGATCTCTTAAATGCAACAGGGAGGGATTCATTGACTTTAGTATTTGTGGAGACTA AAAAGGGTGCAGATTCACTGGAGAACTTCTTATTTCAAGAAAGATATGCATGTACTAGTATTCATGGAGA CAGGTCACAGAAAGATCGAGAGGAGGCCCTTCACCAATTCCGCTCAGGGAGGAAGCCAATTTTAGTGGCT ACAGCTGTGGCAGCAAGAGGTCTAGACATTTCAAATGTGAAACATGTTATCAATTTTGATTTGCCAAGTG ATATTGAAGAATATGTGCATCGTATTGGCCGCACAGGACGCGTAGGAAACCTTGGTCTTGCCACCTCCTT TTTCAATGAGAGGAATTTGAACATTACGAAAGATTTGTTAGATCTCCTCGTTGAGGCTAAACAAGAAGTA CCATCTTGGTTGGAAAGTATGGCTTATGAACACCACTACAAGGGAAACAGCCGGGGACGTTCTAAAAGCA GATTCAGTGGAGGATTTGGTGCCAGAGACTATCGACAGAGCAGTGGTTATGCAAATTCTGGGTTTAATAA TAATCGTGCCAACAGCAGCCGAACTAGTGGTAGTACCCACAACAGAGGATTTGGTGGAGGTGGCTATGGA GGTTTCTACAGCAATGAAGGATATGGAGGAAATTATAACTCCCAGGCAGTTGACTGGTGGGGCAAT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217050 representing NM_001167665 Red=Cloning site Green=Tags(s) MAAENTVGLDQQFAGLDMNSDNQNGGVSTESKGRYIPPHLRNRETSKGVCDKDNSGWSKDKDAYSSFGSR DSRGKPNYFSDRGSGSRGRFDDPGRSDYDCIASRDRSGFGKFERSGYSRWGDKSDEDDWSKPLPPSERLE QELFSGGNAGINFEKYDDIPVEATGNNCPPHIENFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEK RDLMACAQTGSGCTAAFLLPILSQIYTDGPGEALKAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEAR KFSYRSRVRPCVVYGGADTVQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGF EPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEELDK RSFLLDLLNATGRDSLTLVFVETKKGADSLENFLFQERYACTSIHGDRSQKDREEALHQFRSGRKPILVA TAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNLNITKDLLDLLVEAKQEV PSWLESMAYEHHYKGNSRGRSKSRFSGGFGARDYRQSSGYANSGFNNNRANSSRTSGSTHNRGFGGGGYG GFYSNEGYGGNYNSQAVDWWGN SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001167665
ORF Size	1956 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001167665.1, NP_001161137.1
RefSeq Size	3087 bp
RefSeq ORF	1959 bp
Locus ID	100312982
MW	73 kDa

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