Gars (NM_001271139) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GlyRS; RGD1559871
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217147 representing NM_001271139 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGTCTGCTCCCCACGCTGCTCAGAGCCACCCGCGCCGCGCTGCTCCTACAGTCGCCCCGAGTGG TCGCAGCGCCCGCGTCTCAGCGGCTCCTCAGCGCTCCCGCGCAGCCCGCCGCCTCTCCGAGCAGCATGGA CAGCGCCGAGGAGCTGCTAGCCCCATTGCGGCTAGCTGTGCGCCAGCAGGGAGACTTTGTACGGAAGTTA AAAGAAGATAAAGCCCCACAAGTGGATGTGGACAGAGCAGTAGCTGAGCTCAAGGCCCGGAAGAGGGTTC TGGAAGCAAAGGAGCTGGCGTTGCAGCCCAAAGATGACATCGTAGATAGAGCAAAAATGGAGGATACTTT GAAGAGGAGATTTTTCTACGATCAAGCTTTTGCTATTTATGGAGGTGTCAGTGGTTTGTATGACTTTGGG CCAGTGGGATGTGCTTTGAAGAACAATATCATCCAGACCTGGAGGCAGCACTTTATCCAAGAGGAACAGA TCCTGGAAATTGACTGCACCATGCTCACCCCCGAGCCAGTTTTAAAGACCTCTGGCCATGTAGACAAATT TGCTGACTTCATGGTGAAGGACGTGAAGAACGGAGAGTGCTTCCGCGCGGACCACCTGTTGAAAGCTCAT TTACAGAAACTGATGTCAGATAAGAAGTGCTCTGCTGAGAAGAAATCAGAGATGGAAAGTGTCTTGGCCC AGCTTGATAACTATGGACAACAAGAACTGGCGGATCTTTTTGTGAACTATAATGTAAAATCTCCCACCAC TGGCAATGACCTGTCCCCTCCGGTACCTTTTAACTTAATGTTCCAGACCTTCATTGGGCCTGGAGGAAAC ATGCCTGGATATCTGAGACCAGAAACTGCACAGGGAATTTTCCTAAATTTCAAACGACTTTTGGAATTCA ACCAAGGGAAGTTGCCTTTTGCTGCTGCACAGATTGGAAACTCCTTTAGAAATGAGATCTCGCCCCGGTC TGGACTGATCCGAGTCAGGGAATTCACAATGGCAGAGATCGAGCACTTTGTAGATCCCACTGAGAAAGAC CATCCCAAGTTCCCGAGCGTGGCCGACCTCTACCTTTATTTGTACTCTGCCAAAGCCCAGGTCACTGGAC AGTCTGCTCGCAAGATGCGTCTGGGAGATGCTGTTGAACAGGGTGTGATTAACAACTCAGTATTAGGCTA TTTTATTGGCCGCATCTACCTCTACCTCACGAAGGTTGGAATATCTCCTGATAAACTCCGCTTCCGACAG CATATGGAGAATGAGATGGCCCATTATGCCTGCGACTGCTGGGATGCTGAGTCCAAAACGTCCTATGGCT GGATCGAGATTGTTGGATGTGCTGACCGATCCTGCTATGACCTCTCCTGTCATGCTCGAGCCACCAAAGT CCCGCTGGTGGCTGAGAAGCCCCTGAAAGAGCCCAAAACAGTTAACGTTGTACAGTTTGAGCCCAACAAG GGAGCCGTGGGCAAGGCGTACAAGAAAGATGCAAAGCTGGTGCTGGAGTACCTCGGCGCCTGCGATGAGT GCTACATTACCGAGATGGAGCTGCTGCTGAGCGAGAAAGGGGAATTCACTATTGAAACTGAAGGAAAAAC ATTTCAGTTAACAAAAGACATGGTCAGTGTGAAGAGATTCCAGAAAACACTACACGTGGAAGAAGTTGTT CCGAGTGTAATTGAGCCCTCTTTTGGCCTGGGCAGGATCATGTATACCATATTGGAACATACATTCCACG TCCGGGAGGGAGATGAGCAGAGAACGTTCTTCAGTTTCCCTGCTGTGGTTGCTCCATTCAAATGTTCTGT CCTTCCACTGAGCCAAAACCAAGAGTTTATGCCATTTGTCAAGGAATTATCGGAAGCTCTGACTCGAAAT GGCGTGTCTCATAAAGTTGACGATTCCTCTGGGTCTATTGGAAGGCGCTATGCAAGAACTGATGAGATTG GCGTGGCGTTTGGCATCACCATTGACTTTGATACAGTGAACAAGACCCCCCACACTGCAACTCTGAGGGA CCGAGACTCCATGAGACAGATAAGGGCAGAGGTCTCTGAACTGCCCAGTGTGGTCCGTGATCTGGCCAAT GGCAACATCACTTGGGCTGATGTGGAGGCCAGGTACCCCCTCTTTGAAGGGCAAGAGACTGGCAAGAAGG AGACAGTGGAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217147 representing NM_001271139 Red=Cloning site Green=Tags(s) MPCLLPTLLRATRAALLLQSPRVVAAPASQRLLSAPAQPAASPSSMDSAEELLAPLRLAVRQQGDFVRKL KEDKAPQVDVDRAVAELKARKRVLEAKELALQPKDDIVDRAKMEDTLKRRFFYDQAFAIYGGVSGLYDFG PVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAH LQKLMSDKKCSAEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPTTGNDLSPPVPFNLMFQTFIGPGGN MPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPTEKD HPKFPSVADLYLYLYSAKAQVTGQSARKMRLGDAVEQGVINNSVLGYFIGRIYLYLTKVGISPDKLRFRQ HMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPNK GAVGKAYKKDAKLVLEYLGACDECYITEMELLLSEKGEFTIETEGKTFQLTKDMVSVKRFQKTLHVEEVV PSVIEPSFGLGRIMYTILEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRN GVSHKVDDSSGSIGRRYARTDEIGVAFGITIDFDTVNKTPHTATLRDRDSMRQIRAEVSELPSVVRDLAN GNITWADVEARYPLFEGQETGKKETVEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271139
ORF Size	2184 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271139.2, NP_001258068.1
RefSeq Size	2459 bp
RefSeq ORF	2187 bp
Locus ID	297113
UniProt ID	Q5I0G4
MW	81.8 kDa
Gene Summary	Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.[UniProtKB/Swiss-Prot Function]

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