Aplp2 (NM_012906) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	APLP-2; Apph; WALPLP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217188 representing NM_012906 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCACCGGGACCGCGGCCGCTGCGGCCACCGGCAAGCTTCTTGTCCTGCTGCTGCTCGGGCTCA CGGCACCCGCAGCGGCTCTGGCTGGCTATATCGAGGCTCTCGCAGCCAATGCTGGAACAGGCTTTGCGGT TGCTGAACCTCAGATTGCCATGTTCTGTGGGAAGCTGAACATGCATGTGAACATTCAGACTGGGAAGTGG GAGCCTGACCCAACCGGCACCAAGAGCTGCCTGGGAACAAAGGAGGAAGTTCTTCAGTACTGCCAGGAGA TATATCCAGAGCTGCAGATCACAAATGTGATGGAAGCAAACCAGCCAGTCAATATTGATAGTTGGTGCCG AAGGGACAAAAAACAGTGCAGGAGTCACATTGTTATACCATTCAAGTGTCTTGTGGGTGAATTTGTAAGT GATGTTCTGCTAGTTCCAGAAAACTGCCAGTTTTTCCACCAAGAGCGGATGGAGGTGTGTGAGAAGCATC AGCGGTGGCACACAGTCGTCAAGGAGGCATGTCTGACTGAGGGGATGACCTTATATAGCTATGGCATGCT GCTGCCCTGTGGGGTAGACCAGTTCCATGGCACTGAGTATGTGTGCTGCCCTCAGACAAAGGTTGTTGAC TCTGACTCCACTATGTCCAAAGAAGAGGAGGAAGAAGAGGAAGAGGATGAAGAAGAAGACTATGCTCTTG ATAAAAGTGAATTTCCTACCGAAGCAGATCTGGAAGACTTCACAGAAGCAGCTGCAGATGAGGACGAGGA TGAGGAGGAGGAGGAGGAGGAGGAAGGGGAGGAAGTGGTAGAAGACCGTGATTACTACTATGACTCCTTT AAAGGAGATGATTACAATGAGGAGAACCCAACTGAACCCAGCAGCGATGGCACCATCTCAGACAAGGAGA TTGCTCACGATGTTAAAGCTGTCTGCTCCCAGGAGGCCATGACGGGGCCCTGCCGGGCCGTGATGCCTCG TTGGTACTTCGACCTCTCCAAGGGAAAGTGCGTACGCTTTATATATGGCGGCTGCGGCGGCAACAGGAAC AATTTTGAGTCTGAGGATTATTGTATGGCTGTGTGTAAAACGATGATTCCCCCGACTCCCCTGCCAACCA ATGATGTGGACGTGTATTTTGAGACGTCGGCTGATGACAATGAGCACGCCCGCTTTCAGAAGGCTAAGGA GCAGTTGGAAATTCGGCATCGCAGCCGAATGGACAGGGTAAAAAAGGAGTGGGAAGAAGCAGAACTTCAA GCCAAGAACCTCCCCAAGGCAGAGCGACAGACCCTCATTCAGCATTTCCAAGCTATGGTTAAAGCCTTAG AGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTTGTGGAGACCCATTTGGCCCGAGTAGAAGCAATGCTGAA TGACCGTCGGCGCATAGCTCTGGAAAACTACCTGGCTGCCCTGCAGTCTGACCCTCCTCGGCCACATCGC ATTCTTCAAGCTCTTCGTCGTTACGTCCGTGCTGAGAACAAAGATCGCCTGCACACCATTCGTCATTACC AACATGTGTTGGCTGTTGACCCAGAAAAGGCTGCGCAGATGAAATCCCAGGTGATGACACACCTCCATGT CATTGAAGAAAGAAGGAACCAAAGTCTCTCTCTGCTGTACAAAGTGCCTTATGTGGCTCAAGAGATTCAG GAGGAAATTGATGAGCTCCTTCAGGAACAGCGAGCAGATATGGACCAGTTTACCTCCTCCATCTCAGAGA ACCCTGTGGATGTCCGGGTGAGCTCTGAGGAAAGTGAGGAGATCCCGCCGTTCCACCCTTTCCATCCCTT CCCATCCTTGTCTGAGAACGAAGACACTCAGCCGGAGTTGTACCACCCAATGAAAAAAGGATCTGGAATG GCAGAGCAAGATGGGGGCCTGATTGGTGCAGAAGAAAAGGTGATCAACAGTAAGAATAAAATGGATGAAA ATATGGTCATTGACGAGACTCTGGATGTTAAGGAAATGATTTTCAATGCTGAGAGAGTTGGGGGTCTGGA GGAAGAGCCGGACTCTGTGGGGCCTCTACGGGAGGACTTCAGTTTGAGCAGCAGTGCCCTTATTGGCTTG CTGGTCATCGCGGTGGCCATTGCTACAGTCATCGTCATCAGCCTGGTGATGCTGAGGAAGAGGCAATACG GCACCATCAGCCATGGCATTGTGGAGGTTGATCCAATGCTCACCCCAGAAGAGCGTCACTTGAACAAGAT GCAGAACCACGGCTATGAAAACCCAACCTACAAATACCTGGAGCAGATGCAGATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217188 representing NM_012906 Red=Cloning site Green=Tags(s) MAATGTAAAAATGKLLVLLLLGLTAPAAALAGYIEALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTGKW EPDPTGTKSCLGTKEEVLQYCQEIYPELQITNVMEANQPVNIDSWCRRDKKQCRSHIVIPFKCLVGEFVS DVLLVPENCQFFHQERMEVCEKHQRWHTVVKEACLTEGMTLYSYGMLLPCGVDQFHGTEYVCCPQTKVVD SDSTMSKEEEEEEEEDEEEDYALDKSEFPTEADLEDFTEAAADEDEDEEEEEEEEGEEVVEDRDYYYDSF KGDDYNEENPTEPSSDGTISDKEIAHDVKAVCSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRN NFESEDYCMAVCKTMIPPTPLPTNDVDVYFETSADDNEHARFQKAKEQLEIRHRSRMDRVKKEWEEAELQ AKNLPKAERQTLIQHFQAMVKALEKEAASEKQQLVETHLARVEAMLNDRRRIALENYLAALQSDPPRPHR ILQALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYVAQEIQ EEIDELLQEQRADMDQFTSSISENPVDVRVSSEESEEIPPFHPFHPFPSLSENEDTQPELYHPMKKGSGM AEQDGGLIGAEEKVINSKNKMDENMVIDETLDVKEMIFNAERVGGLEEEPDSVGPLREDFSLSSSALIGL LVIAVAIATVIVISLVMLRKRQYGTISHGIVEVDPMLTPEERHLNKMQNHGYENPTYKYLEQMQI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012906
ORF Size	2295 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012906.1, NP_037038.1
RefSeq Size	3644 bp
RefSeq ORF	2298 bp
Locus ID	64312
MW	86.9 kDa
Gene Summary	may contribute to membrane cytoplasmic junctions in sperm membranes [RGD, Feb 2006]

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