Aldh1l2 (NM_001191778) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1309458
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217334 representing NM_001191778 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTGGCGGGGTAGCCAGGCGCTACGGCACTTCTCCACCGGCCGGGTTTATTTCAAAAACAAGTTGA AGTTGGCACTTATTGGCCAGAGCCTGTTTGGACAGGAAGTCTACAGCCACCTCCTGAAAGAGGGTCACCG AGTAGTGGGAGTGTTCACAGTGCCAGACAAGGATGGAAAGGCCGATCCACTGGCTTTGGCCGCAGAGAAA GATGGGACCCCTGTGTTCAAGTTCCCTCGATGGAGAGTCAAGGGTAAAACCATCAAGGAGGTAGCAGAAG CCTATCAGTCGGTAGGTGCTGAGCTCAACGTGCTTCCCTTCTGCACGCAGTTCATCCCCATGGACATCAT CGACAGCCCAGAGCACGGCTCCATCATCTACCACCCCTCACTCCTACCCAGGCACAGAGGGGCTTCTGCC ATCAACTGGACTCTAATCATGGGAGATAAGAAAGCTGGGTTTTCTGTTTTCTGGGCAGATGATGGTTTAG ATACAGGACCCATCCTTCTTCAGAGATCGTGTGACGTCAAACCCAATGACACAGTGGACTCGCTTTATAA CCGGTTCCTTTTTCCTGAGGGGATCAAGGCCATGGTAGAAGCTGTCCAACTCATAGCTGATGGAAAGGCG CCTCGCACTCCCCAGCCGGAAGAGGGGGCCACATATGAAGGCATCCAGAAGAAGGAAAACGCTGAGATTC CTTGGGACCAGCCTGCTGAAGTTTTGCACAACTGGATTCGAGGTCATGATAAGGTCCCTGGAGCCTGGGC AGAGATAGACGGACAGATGGTGACTTTCTACGGCTCATCACTGTTGACAAGTTCTGTGCCTGCTGGAGAA CCACTGGACATTAGAGGTGCCAAGAAGCCTGGTCTTGTCACCAAAAATGGCCTTGTTCTTTTTGGGAATG ATGGGAAAGCGCTGATGGTGAGAAACCTGCAGTTTGAAGACGGGAAGATGATTCCCGCCTCCCAGTACTT TTCGTCGGGTGAGACATCGGTGGTAGAACTTACGGCTGAGGAGCTGAAGGTGGCAGAGACCATAAAGGTC ATCTGGGCTAGAATCTTAAGCAACGTCCCAGTGATTGAGGACTCCACGGACTTCTTTAAATCTGGAGCGA GCTCAATGGATGTTGCGAGGCTGGTGGAAGAGATCAGACAGAAGTGCGGTGGGCTTCAGCTGCAGAACGA AGATGTCTACATGGCCCGCAAGTTTGGAGATTTCATCCAAAAGGTTGTGAGGAGACTAAGAGGAGAGGAC CAAGAGGCTGAGCTGGTGGTGGATTATGTTTCCAAGGAGGTAAACGAGATGACAGTGAAGATGCCCTACC AGTGTTTCATAAACGGACAGTTTGTAGATGCCGAGGATGGAGAGACTTACGCCACCATCAACCCCACAGA CGGAACTACGCTATGCAGAGTGTCCTACGCTTCTTTAGCGGATGTTGACAGAGCAGTAGCCGCGGCCAAG GACGCCTTTGAGAATGGCGAATGGGGAAGGATGAACGCCAGGGACAGAGGAAGACTCATGTACAGACTCG CGGACCTAATGGAAGAAAACCAGGAGGAGCTGGCGACCATAGAAGCGCTGGATTCCGGGGCTGTCTACAC CCTGGCTCTGAAGACTCATATTGGAATGTCCGTGCAGACCTTCAGATACTTCGCAGGCTGGTGTGACAAA ATTCAGGTAACAAAGGAACCAGAGAACCAGGCTAGAATTCATGACACTCTGGGGATGAGCTCGGAAGAAC CAAAAGGTGTCTGCGCCATCATCATTCCCTGGAACTACCCACTAATGATGCTGGCCTGGAAGAGTGCAGC CTGCCTGGCAGCAGGCAACACCCTAGTACTCAAGCCAGCGCAGGTCACACCCTTGACAGCTCTGAAGTTT GCAGAGCTGACGGTGAAAGCAGGCTTTCCGAAGGGGGTAATCAACATCATTCCTGGCTCAGGGGGTGTGG CAGGACAGCGGCTCTCTCAGCACCCTGACATCCGCAAGCTTGGTTTCACTGGTTCCACCTCTGTGGGCAA ACAGATCATGAAAAGCTGTGCTGTGAGCAACCTGAAGAAAGTTTCCCTTGAGCTTGGTGGCAAATCCCCA CTTATAATATTCAGCGACTGTGAACTTGACAAGGCCGTCCGGATGGGCATGGGCGCAGTATTCTTCAATA AAGGAGAGAACTGCATTGCAGCTGGCCGGCTGTTCGTGGAAGAGGCCATCCACGATGAATTCGTCACCAG AGTGGTGGAAGAGATTAAAAAGATGAAAATTGGTGATCCCCTGGACAGATCTACGGACCACGGGCCCCAG AACCACAAAGCCCATTTGGAAAAGCTGCTGCAGTACTGCGAGACGGGGGTGAAGGAGGGGGCCACACTGG TGTACGGAGGAAGACAGGTACAGAGGCCAGGCTTTTTCATGGAGCCCACTGTGTTCACAGGCGTGGAGGA CCACATGTACCTCGCCAAAGAGGAATCCTTCGGGCCTATCATGGTCATCTCTAAATTCCAAAATGGGGAC ATCGATGGGGTGCTGCAGAGAGCAAACAACACTGAGTACGGCCTGGCCTCAGGGGTCTTCACGAGAGACA TCAGCAAAGCCATGTACGTGAGTGACAGGCTAGAGGCGGGAACGGTTTTCATTAACACCTACAACAAGAC GGACGTGGCAGCCCCATTTGGTGGAGTGAAGCAGTCTGGCTTTGGAAAAGACTTAGGTGAGGAGGCCCTG AACGAATACCTGAAAACCAAAACAGTGACCCTCGAGTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217334 representing NM_001191778 Red=Cloning site Green=Tags(s) MLWRGSQALRHFSTGRVYFKNKLKLALIGQSLFGQEVYSHLLKEGHRVVGVFTVPDKDGKADPLALAAEK DGTPVFKFPRWRVKGKTIKEVAEAYQSVGAELNVLPFCTQFIPMDIIDSPEHGSIIYHPSLLPRHRGASA INWTLIMGDKKAGFSVFWADDGLDTGPILLQRSCDVKPNDTVDSLYNRFLFPEGIKAMVEAVQLIADGKA PRTPQPEEGATYEGIQKKENAEIPWDQPAEVLHNWIRGHDKVPGAWAEIDGQMVTFYGSSLLTSSVPAGE PLDIRGAKKPGLVTKNGLVLFGNDGKALMVRNLQFEDGKMIPASQYFSSGETSVVELTAEELKVAETIKV IWARILSNVPVIEDSTDFFKSGASSMDVARLVEEIRQKCGGLQLQNEDVYMARKFGDFIQKVVRRLRGED QEAELVVDYVSKEVNEMTVKMPYQCFINGQFVDAEDGETYATINPTDGTTLCRVSYASLADVDRAVAAAK DAFENGEWGRMNARDRGRLMYRLADLMEENQEELATIEALDSGAVYTLALKTHIGMSVQTFRYFAGWCDK IQVTKEPENQARIHDTLGMSSEEPKGVCAIIIPWNYPLMMLAWKSAACLAAGNTLVLKPAQVTPLTALKF AELTVKAGFPKGVINIIPGSGGVAGQRLSQHPDIRKLGFTGSTSVGKQIMKSCAVSNLKKVSLELGGKSP LIIFSDCELDKAVRMGMGAVFFNKGENCIAAGRLFVEEAIHDEFVTRVVEEIKKMKIGDPLDRSTDHGPQ NHKAHLEKLLQYCETGVKEGATLVYGGRQVQRPGFFMEPTVFTGVEDHMYLAKEESFGPIMVISKFQNGD IDGVLQRANNTEYGLASGVFTRDISKAMYVSDRLEAGTVFINTYNKTDVAAPFGGVKQSGFGKDLGEEAL NEYLKTKTVTLEY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191778
ORF Size	2769 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191778.1, NP_001178707.1
RefSeq Size	3502 bp
RefSeq ORF	2772 bp
Locus ID	299699
MW	101.7 kDa

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