Nlrp12 (NM_001169142) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Nalp12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217442 representing NM_001169142 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGCCAGCTACAGCCAAGGATGCCCTCCATCGACTGTCCACCTACCTGGAAGAACTTGAGGCTGGGG AACTGAAGAAATTCAAATTATACCTGGGGATTGCGGAGGAACTGGGCCAGGACAAGATCCCCAGGGGACG AATGGAGATGGCTGGTCCTCTGGAAATGGCCCAATTGATGGTGGCCCATATGGGGACAAAGGAGGCTTGG CTTCTGGCTCTCAGCACCTTTGAGAGGATCCACAGGAAGGACCTGTGGGAGCGAGGACAGGGAGAAGACC TGGTGAGGGTGACTCCAAGTAATGGTCTATGCTCTCTTGAGAGCCAGTCAACGTGCCTTTCGGACGTCTC TCCTGATGCTCCAAGAAAAGATCCCCAGATAACCTACAAAGACTATGTCCGAAGGAAATTCCGGCTAATG GAAGACCGCAATGCGCGACTAGGCGAATGTGTGAACTTAAGTCATCGTTACACTCGGCTTCTCCTAGTAA AGGAACACTCAAATCCTATCTGGGCACAGCAAAAACTTCTGGAAACAGGGTGGGAACACTCCAGAACCAG GGGCCACCAGGCTAGCCCTATCCAAATGGAGACCCTCTTTGAGCCAGATGAAGAACGCCCGGAGCCACCA CGCACAGTGGTATTACAAGGGGCAGCGGGGATGGGGAAGTCCATGTTGACCCACAAAGTGATGTTGGACT GGGCCGATGGGAGACTCTTCCAAGACCAGTTCGATTATGTCTTCTATATCAGCTGCAGGGAGTTGAATAG AAGCCACACCCAGTGCAGTGTACATGACCTCCTCTCCAGCTGCTGGCCAGAGCATGGTGCACCCCTCGAG GACCTCATCCGGGCTCCTGATCGTCTTCTATTCATCATCGATGGCTTCCATGAGCTTCATCCCTCTTTCC ATGACGTTCAGGGTCCCTGGTGCCACTGCTGGGAGGAGAAACGACCCACGGAACTCCTCCTCGGAAGCCT GATTCGGAGGTTGCTTCTGCCCCAGCTCTCTTTGCTCATCACCACACGACCCTGTGCACTGGAGAAGCTG CACGGCTTGTTGGAACACCCTAGGCACGTGGAGATCCTGGGCTTCTCCGAGGCAGAGAGGGAGGAATATT TCTACAGATATTTCCACAACACTGGACAAGCGAGCCAAGTGTTCAGCTTCATGAGGGACTATGAGCCCCT CTTTACCATGTGTTTTGTTCCCATGGTGTCCTGGGTGGTCTGCACCTGCTTAAAGCAGCAGCTAGAAAGT GGGGAGCTTTTAAGACAAACATCTAGGACCACCACAGCGGTTTATATGTTCTACCTTCTGAGCCTGATGC AGCCCAAGCCAGGGACCCCAACCTTCAAAGTCCCAGCCAACCAGAGAGGCCTGGTCTCTCTGGCTGCAGA GGGCCTCTGGAATCAGAAGATTCTCTTCGAGGAAGAGGATCTTGGGAAACATGGCCTAGATGGAGCAGAG GTCTCCACCTTCCTCAACGTGAACATATTCCAGAAGGGCATCAAGTGTGAGAAATTCTACAGCTTCATCC ACCTGAGTTTCCAGGAATTCTTCACAGCCATGTACTGTGCACTGCACGGCAGAGAGGCGGTGAGGAGAGC ACTGGCTGAGTATGGTTTCTCAGAAAGGAACTTCTTGGCCCACACCGTCCGCTTTCTCTTTGGCCTCCTC AATGAAGAGATGAGATGCTACCTCGAGAGGAATCTCGGCTGGACCATCTCCCCTCAGGTCAAGGAGGAAG CATTGGCGTGGATCCAAAACAAGGCTCGAAGTGAGGGCTCCACCCTGCAGCATGGCTCCCTGGAGCTTCT CAGCTGCTTGTACGAGATCCAGGAGGAAGACTTCATCCAGCAGGCCCTGAGCCACTTTCAGGTGGTTGTT GTCAGAAACCTCTCAACGAAGATGGAGCACGTGGTCTGCTCGTTTTGTGCGAGGTACTGCAGAGGCACAG AAGTGCTTCACTTGTATGGGAGTGCCTATAGTACAGGCGCAGAGGATGGCCCGCCAGAACCTCCAGGAGC CCAGACGCAGTCCACACACTCACAGGAAAGGAACATCCTTCCTGACATTTACAGTGCATACCTCTCAGCA ACCATCTGCACCAACTCAAACCTGATCGAGCTGGCCTTGTATCGAAACGCCTTGGGCAGCCAGGGCGTGA GGCTGCTCTGTCAAGGCCTCCGACATGCAAACTGCAAGCTTCAGAACCTGAGGCTGAAGAGGTGTCACAT CTCCGGATCAGCCTGCCAGGATCTCGCAGCGGCTATCATCGCCAATAGGAATTTAATCAGGCTGGACCTC AGTGGCAACAGCATTGGAGTGCTAGGCCTAGAGCTGCTCTGTGAGGGGTTACAGCACCCCATGTGTAGGC TGCAGATGATCCAGCTGAGGAAGTGTTTGCTGGAGGCTGCGGCTGGCCGAGCACTGGCCTCTGTTCTCAG CAACAACTCACATCTTGTAGAACTGGATCTGACAGGAAACCCCTTGGAGGATTTGGGGCTGAAGTTACTG TGTCAAGGGCTGAGGCACCCAGTCTGCAGGCTGCGGACCTTGTGGCTGAAGATCTGCCACCTTGGACAAG CTTCCTGTGAAGATCTGGCTTCTACTCTCAAAATGAACCAGAGCCTGATGGAGCTGGACCTGGGTCTGAA TGACCTTGGGGATTCTGGGGCGCTTCTGCTGTGTGAGGGCCTCAGGCATCCAGATTGCAAACTCCAGACC CTCCGGCTGGGCATTTGCCGACTGGGCTCAGACGCGTGTGCAGGGGTTGCTAGTGTGCTCCAGGTCAACA CGTGCCTCCGAGAGCTGGACCTGAGCTTCAATGACTTGGGAGACAGGGGCCTGTGGCTGCTGGGGGAAGG ACTGAGGCACCAGACCTGCAGACTCCAGAAGCTCTGGCTGGACAGTTGCGGCCTCACTTCCAAAGCATGT GAGGACCTGTCTTCAGTCCTGGGAATCAGTCAGACCCTGAATGAGCTTTATCTGACCAATAATGCTCTGG GGGACACTGGTGTTCGTCTGCTGTGCAAGAGGCTGAGGCATCCGGGGTGCAAGCTTCGTGTCCTGTGGCT TTTCGGAATGGACCTGAACAAAGTGACCCACAGGAGGATGGCGGCACTTCGAGTCACAAAACCATACCTA GATATTGGGTGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217442 representing NM_001169142 Red=Cloning site Green=Tags(s) MLPATAKDALHRLSTYLEELEAGELKKFKLYLGIAEELGQDKIPRGRMEMAGPLEMAQLMVAHMGTKEAW LLALSTFERIHRKDLWERGQGEDLVRVTPSNGLCSLESQSTCLSDVSPDAPRKDPQITYKDYVRRKFRLM EDRNARLGECVNLSHRYTRLLLVKEHSNPIWAQQKLLETGWEHSRTRGHQASPIQMETLFEPDEERPEPP RTVVLQGAAGMGKSMLTHKVMLDWADGRLFQDQFDYVFYISCRELNRSHTQCSVHDLLSSCWPEHGAPLE DLIRAPDRLLFIIDGFHELHPSFHDVQGPWCHCWEEKRPTELLLGSLIRRLLLPQLSLLITTRPCALEKL HGLLEHPRHVEILGFSEAEREEYFYRYFHNTGQASQVFSFMRDYEPLFTMCFVPMVSWVVCTCLKQQLES GELLRQTSRTTTAVYMFYLLSLMQPKPGTPTFKVPANQRGLVSLAAEGLWNQKILFEEEDLGKHGLDGAE VSTFLNVNIFQKGIKCEKFYSFIHLSFQEFFTAMYCALHGREAVRRALAEYGFSERNFLAHTVRFLFGLL NEEMRCYLERNLGWTISPQVKEEALAWIQNKARSEGSTLQHGSLELLSCLYEIQEEDFIQQALSHFQVVV VRNLSTKMEHVVCSFCARYCRGTEVLHLYGSAYSTGAEDGPPEPPGAQTQSTHSQERNILPDIYSAYLSA TICTNSNLIELALYRNALGSQGVRLLCQGLRHANCKLQNLRLKRCHISGSACQDLAAAIIANRNLIRLDL SGNSIGVLGLELLCEGLQHPMCRLQMIQLRKCLLEAAAGRALASVLSNNSHLVELDLTGNPLEDLGLKLL CQGLRHPVCRLRTLWLKICHLGQASCEDLASTLKMNQSLMELDLGLNDLGDSGALLLCEGLRHPDCKLQT LRLGICRLGSDACAGVASVLQVNTCLRELDLSFNDLGDRGLWLLGEGLRHQTCRLQKLWLDSCGLTSKAC EDLSSVLGISQTLNELYLTNNALGDTGVRLLCKRLRHPGCKLRVLWLFGMDLNKVTHRRMAALRVTKPYL DIGC SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001169142
ORF Size	3162 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001169142.1, NP_001162613.1
RefSeq Size	3441 bp
RefSeq ORF	3165 bp
Locus ID	292541
MW	119.3 kDa

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