Slc12a7 (NM_001013144) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Kcc4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217462 representing NM_001013144 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCACGAACTTTACGGTGGTGCCGGTGGAGGCGCGCGCCGACGGCGCCGGGGACGAAGCTGCTGAGC GCACGGAAGAACCCGGGTCTCCCGAGAGCGCGGATCCTGCCTGCCCTACGCCGGGAGATGGAAACCCCAG GGAGAACAGCCCATTCATCAATAATGTGGAGGTGGAAAGAGAGAGCTACTTCGAGGGGAAGAACATGGCG CTTTTTGAGGAAGAGATGGACAGCAACCCCATGGTATCATCACTGCTGAACAAGCTGGCCAACTATACCA ACCTGAGCCAGGGTGTGGTAGAGCATGAGGAAGATGAGGACAGCCGGAGGCGGGAGATCAAGGCCCCACG CATGGGCACCTTCATCGGAGTCTACCTACCGTGCCTGCAGAACATCTTGGGTGTAATCCTTTTCCTGCGC CTGACCTGGATTGTGGGGGCGGCTGGCGTTCTGGAGTCCTTCCTCATCGTGGCCATGTGCTGCACCTGTA CAATGCTGACAGCCATCTCCATGAGTGCCATCGCTACCAACGGTGTGGTCCCAGCGGGAGGCTCATACTA CATGATCTCCCGTTCGCTGGGGCCTGAGTTTGGAGGGGCTGTTGGCCTCTGCTTCTACTTGGGCACAACG TTCGCAGGCGCCATGTACATCCTGGGAACCATCGAGATCTTCCTGACCTACATCTCTCCAAGTGCAGCCA TCTTCCAGGCAGAGACAGCAGACGGCGAGGCGGCCGCGCTGCTGAACAACATGCGTGTGTATGGCAGCTG CGCACTGGCGCTCATGGCCGTGGTGGTCTTCGTCGGAGTCAAATATGTCAACAAGCTGGCCCTGGTCTTC TTAGCCTGTGTTGTGCTTTCTATCCTGGCCATCTATGCTGGTGTCATCAAGACAGCCTTCGCCCCACCTG ACATCCCGGTCTGCCTTCTAGGGAACCGCACGCTGGCGAATCGCAACTTTGATACCTGTGCCAAAATGCA GGTTGTCAGCAATGGCACAGTCACCACCGCGCTCTGGCGCCTCTTCTGCAACGGCTCCAGCTTGGGTGCC TCCTGCGATGAGTACTTTGTGCAGAACAATGTCACTGAGATCCAGGGCATTCCTGGTGTGGCCAGTGGTG TCTTCCTGGATAACCTGTGGAGCACGTATTCGGACAAGGGGGCATTTGTGGAAAAGAAGGGCGTGTCCTC GGTGCCTGTGTCCGAGGAGAGCCGGCCTGGTGGATTGCCATATGTCCTCACAGACATCATGACCTACTTC ACCATGCTAGTCGGCATCTACTTCCCCTCCGTAACGGGGATCATGGCAGGATCCAACCGCTCCGGGGACC TCAAAGATGCCCAGAAGTCCATCCCAACAGGGACCATTCTGGCCATTGTGACTACATCTTTCATTTATCT TTCCTGCATTGTGCTGTTTGGGGCCTGCATCGAGGGTGTAGTCTTGCGAGATAAGTTTGGGGAGGCCCTG CAAGGGAACTTGGTCATTGGCATGCTGGCTTGGCCATCTCCCTGGGTCATCGTGATTGGCTCCTTCTTCT CCACCTGTGGTGCTGGCCTGCAGAGCCTAACTGGGGCACCCCGCCTGCTGCAGGCCATTGCACGCGACGG CATCATCCCCTTCCTGCAGGTGTTTGGCCATGGAAAGGCCAACGGGGAGCCCACGTGGGCCCTGCTGCTC ACGGCCCTCATCTGTGAGACCGGCATCCTCATCGCCTCCCTGGACAGCGTGGCTCCCATCCTGTCCATGT TCTTCCTCATGTGCTACATGTTCGTCAACCTGGCCTGTGCTGTTCAGACCCTGCTGCGCACACCCAACTG GCGTCCACGTTTCAAGTTCTACCACTGGACCCTCTCCTTCCTTGGGATGAGTCTCTGCCTTGCGCTCATG TTCATCTGCTCCTGGTACTACGCCCTCTTCGCCATGCTCATCGCGGGCTGCATCTACAAGTACATCGAGT ACCGAGGGGCTGAGAAGGAGTGGGGGGATGGCATCAGGGGCCTGTCACTGAATGCTGCCCGCTATGCCCT GCTGCGTGTGGAACATGGGCCCCCACATACCAAGAACTGGAGGCCCCAGGTATTGGTGATGCTGAACCTG GACTCTGAGCAGTGTGTAAAGCACCCCCGCCTGCTGTCCTTCACCTCTCAGCTGAAGGCTGGCAAGGGCC TGACCATCGTGGGATCTGTGCTGGAGGGCACCTACTTAGACAAGCATGTGGAGGCCCAGCGGGCTGAGGA GAATATCCGGTCTCTGATGAGTGCAGAGAAGATGAAAGGCTTCTGCCAGCTGGTGGTGTCGTCCAACCTG CGAGACGGTGCATCCCACTTGATCCAGTCAGCTGGCCTTGGTGGCATGAAGCACAACACTGTCCTCATGG CCTGGCCAGAGGCTTGGAAGCAGGCAGATAACCCTTTCTCCTGGAAGAACTTTGTGGACACTGTCCGTGA CACTACAGCAGCACATCAGGCACTGTTGGTGGCCAAGAACATCGACTTGTTCCCGCAGAACCAGGAGCGC TTCAGCGACGGCAACATCGATGTGTGGTGGATCGTGCACGATGGGGGGATGCTCATGCTTCTGCCGTTCC TGCTGCGCCAGCACAAGGTGTGGCGAAAGTGCCGGATGCGCATCTTCACGGTCGCCCAGGTGGACGATAA CAGCATCCAGATGAAGAAGGACCTGCAGATGTTCCTGTACCATCTCAGGATCAGCGCCGAGGTGGAGGTG GTAGAGATGGTTGAAAATGACATTTCTGCATTCACCTATGAGAAGACGCTAATGATGGAGCAGAGGTCAC AGATGCTGAAACAGATGCAGTTGTCAAAGAATGAGCGGGAGAGAGAGGCCCAGCTGATTCACGACAGGAA CACTGCATCCCACACCGTAGCAACGGCTAGAACTGAAGCCCCACCAACACCTGACAAAGTGCAGATGACA TGGACGAAAGAGAAACTCATTGCAGAGAAACACAGGAACAAGGACACCGGCACATCAGGCTTCAAAGACC TTTTCAGCCTAAAGCCGGACCAGTCCAACGTCAGAAGGATGCATACTGCTGTGAAGCTCAATGGTGTGGT TCTCAACAAGTCCCAGGATGCTCAACTCGTCCTGCTGAATATGCCAGGCCCCCCAAAAAGTCGGCAGGGC GACGAGAACTACATGGAATTTCTTGAGGTCTTGACCGAAGGGCTGAACAGGGTCCTCCTGGTCAGGGGTG GTGGCCGGGAAGTCATCACCATCTACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217462 representing NM_001013144 Red=Cloning site Green=Tags(s) MPTNFTVVPVEARADGAGDEAAERTEEPGSPESADPACPTPGDGNPRENSPFINNVEVERESYFEGKNMA LFEEEMDSNPMVSSLLNKLANYTNLSQGVVEHEEDEDSRRREIKAPRMGTFIGVYLPCLQNILGVILFLR LTWIVGAAGVLESFLIVAMCCTCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTT FAGAMYILGTIEIFLTYISPSAAIFQAETADGEAAALLNNMRVYGSCALALMAVVVFVGVKYVNKLALVF LACVVLSILAIYAGVIKTAFAPPDIPVCLLGNRTLANRNFDTCAKMQVVSNGTVTTALWRLFCNGSSLGA SCDEYFVQNNVTEIQGIPGVASGVFLDNLWSTYSDKGAFVEKKGVSSVPVSEESRPGGLPYVLTDIMTYF TMLVGIYFPSVTGIMAGSNRSGDLKDAQKSIPTGTILAIVTTSFIYLSCIVLFGACIEGVVLRDKFGEAL QGNLVIGMLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIIPFLQVFGHGKANGEPTWALLL TALICETGILIASLDSVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSLCLALM FICSWYYALFAMLIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALLRVEHGPPHTKNWRPQVLVMLNL DSEQCVKHPRLLSFTSQLKAGKGLTIVGSVLEGTYLDKHVEAQRAEENIRSLMSAEKMKGFCQLVVSSNL RDGASHLIQSAGLGGMKHNTVLMAWPEAWKQADNPFSWKNFVDTVRDTTAAHQALLVAKNIDLFPQNQER FSDGNIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQVDDNSIQMKKDLQMFLYHLRISAEVEV VEMVENDISAFTYEKTLMMEQRSQMLKQMQLSKNEREREAQLIHDRNTASHTVATARTEAPPTPDKVQMT WTKEKLIAEKHRNKDTGTSGFKDLFSLKPDQSNVRRMHTAVKLNGVVLNKSQDAQLVLLNMPGPPKSRQG DENYMEFLEVLTEGLNRVLLVRGGGREVITIYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001013144
ORF Size	3249 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001013144.2, NP_001013162.2
RefSeq Size	5003 bp
RefSeq ORF	3252 bp
Locus ID	308069
UniProt ID	Q5RK27
MW	119.4 kDa
Gene Summary	Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.