Pcdh19 (NM_001169129) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1565392
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217472 representing NM_001169129 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCTCTCCTGCTGCCGGTGCTATTGCTGCTGGCTGTACTATGGACGCAGGCGGCCGCCCTGATTA ACCTTAAATACTCAGTAGAAGAGGAGCAGCGCGCCGGGACTGTGATCGCTAATGTGGCCAAGGACGCGCG GGAGGCAGGCTTCGCGATGGATACCCGACAGGCTTCTGCTTTCCGCGTGGTGTCCAACTCGGCTCCGCAC CTGGTGGACATCAACCCCAGTTCGGGCCTCTTGGTCACCAAGCAGAAGATTGACCGAGACCTGCTGTGCC GCCAGAGCCCCAAGTGTATCATCTCGCTAGAGGTCATGTCCAGCTCAATGGAAATCTGTGTGATTAAGGT GGAAATCAAGGACCTGAACGACAATGCTCCCAGTTTTCCGGCCGCACAGATTGAACTGGAGATCTCTGAG GCAGCCAGCCCCGGCACGCGTATCCCACTGGACAGCGCATATGACCCGGACTCAGGCAGTTTCGGTGTGC AGACCTACGAGCTCACGCCCAACGAGCTGTTTGGCCTGGAAATAAAGACGCGGGGTGACGGTTCGCGCTT TGCTGAACTCGTGGTGGAGAAGAGCCTGGACCGCGAGACACAGTCACACTACAGCTTTCGCATTACGGCG CTCGACGGAGGCGACCCACCACACATGGGCACAGTTGGCCTCACCATCAAGGTGACCGACTCGAATGACA ACAACCCGGTGTTTGGTGAGTCCACTTACTCCGTGAGCGTGCCTGAAAACTCACCTCCCAATACACCTGT CATCCGCCTCAATGCCAGCGATCCAGATGAGGGCACCAACGGCCAAGTAGTCTACTCTTTCTATGGTTAT GTCAATGACCGCACGCGTGAACTCTTCCAAATTGACCCACACAGCGGCCTGGTCACTGTCACCGGTGCGC TAGACTATGAAGAGGGCCACGTGTACGAACTGGATGTGCAAGCCAAAGACCTGGGGCCCAACTCTATCCC TGCACACTGCAAAGTCACTGTCAGCGTGCTAGACACTAACGATAACCCGCCAATTATCAACCTTCTGTCG GTCAATAGCGAGCTTGTGGAGGTGAGCGAGAGCGCCCCCCCGGGCTATGTGATTGCTCTGGTTCGGGTGT CTGATCGCGACTCCGGTCTAAATGGACGTGTGCAGTGCCGCTTGCTGGGCAATGTCCCCTTTAGGCTGCA GGAGTATGAGAGCTTCTCCACTATTCTTGTTGATGGACGGCTGGATCGAGAGCAGCATGACCAATACAAT CTTACTATTCAGGCTCGAGACAGCGGTGTGCCTATGCTGCAAAGTGCCAAGTCTTTTACTGTGCGTATCA CAGACGAGAATGATAACCACCCACACTTCTCTAAGCCTTACTATCAAGTCATTGTACAGGAGAACAACAC TCCTGGCGCCTATCTGCTCTCTGTGTCTGCCCGTGACCCTGACTTGGGTCTCAATGGCAGCGTCTCCTAC CAAATTGTGCCATCACAGGTGCGAGACATGCCAGTCTTCACCTATGTCTCTATCAATCCCAACTCTGGTG ACATCTATGCGCTAAGATCCTTTAACCATGAGCAGACCAAGGCATTTGAATTCAAAGTCCTGGCCAAGGA TGGTGGCATGCCCTCCCTGCAAAGCAATGCCACTGTGAGGGTCATTATCCTCGACGTCAACGACAATACC CCGGTTATCACTGCCCCACCTCTGATCAATGGCACTGCTGAAGTCTACATTCCTCGAAATTCTGGCATAG GCTATTTGGTGACTGTAGTTAAGGCAGATGATTATGATGAGGGCGAAAATGGCCGAGTCACCTATGACAT GACAGAAGGTGACCGAGGTTTCTTCGAAATAGACCAGGTCAATGGAGAAGTCAGAACCACTCGTACCTTT AATGAGAACTCCAAGGCTTCCTATGAGCTTATAGTGGTGGCCCATGACCATGGCAAGACATCTCTTTCTG CTTCTGCCCTTGTCCTAATCTACCTGTCCCCAGCTCTTGATGCTCAAGAGTCAATGGGCTCTGTGAACTT ATCCCTGATTTTCATTATTGCTCTGGGCTCCATTGCCGGCATTCTCTTTGTCACTATGATCTTCGTGGCA ATCAAGTGCAAGCGTGACAACAAAGAGATCCGGACCTACAATTGCAGAATCGCTGAGTACTCCTATGGGC ATCAAAAGAAATCAAGTAAGAAGAAAAAAATTAGTAAGAATGACATCCGCCTTGTACCTCGGGACGTGGA GGAGACAGACAAGATGAATGTTGTCAGTTGCTCCTCCCTTACTTCGTCCCTCAACTATTTCGACTACCAC CAGCAGACACTGCCCCTGGGCTGCCGTCGCTCTGAGAGCACTTTCCTGAATGTGGAGAACCAGAATACCC GCAACACCACTGCCAACCACATCTACCATCACTCCTTCAACAGCCAGGGCCCACAGCAGCCAGACCTGAT CATCAATGGTGTGCCCCTGCCTGAGACTGAAAACTATTCCTTTGACTCTAACTACGTGAATAGCCGTGCC CATTTAATCAAAAGCAGCTCCACCTTCAAGGACCTAGAGGGCAACAGCCTGAAGGATAGTGGGCATGAGG AGAGTGACCAGACCGACAGTGAACATGATGTCCAGAGGAGCCTGTACTGCGATACTGCCGTGAATGATGT TCTCAATACCAGCGTGACCTCCATGGGATCTCACATGCCTGACCATGACCAGAATGACGGATTTCATTGC AGGGAAGAGTGCCGGATTCTTGGCCACTCTGATAGATGCTGGATGCCCCGGAATCCCATGCCCACTCGCT CCAAGTCGCCTGAGCACATGAGGAACATCATCGCTCTGTCCATTGAAGCTACCGCTGCTGATGTTGAAGC TTATGACGACTGCGGCCCCACCAAGCGGACTTTTGCAACCTTCGGAAAAGATGTCAGTAGCCACCCAGCT GAGGAGCGGTCCATCCTGAAAGGCAAGCGGACTGTCGATGTGACCATCTGCAGCCCCAAAGTCAACAGCG CTATCCGGGAGGCGGGCAATGGCTGTGAAGCTATTAGCCCCGTCACCTCCCCACTCCATCTGAAGAGCCC TCTGCCTACCAAGCCTTCCATGTCTTACACCGTCGCCCTGGCTCCTCCAGCTCATGATCTGGATCACCAT GCCAACACTGGCGCCTCTCGTCCTTCTGAAGCTGAGCCCCGTGGAGCCGACAGCGAGAAAGTCATGCACG AGGTCAATCCTATTCTGAAGGAAGGGCGTGACAAGGAATCTCCCGCCGTGAAGCGTCTGAAGGATATCAT TCTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217472 representing NM_001169129 Red=Cloning site Green=Tags(s) MESLLLPVLLLLAVLWTQAAALINLKYSVEEEQRAGTVIANVAKDAREAGFAMDTRQASAFRVVSNSAPH LVDINPSSGLLVTKQKIDRDLLCRQSPKCIISLEVMSSSMEICVIKVEIKDLNDNAPSFPAAQIELEISE AASPGTRIPLDSAYDPDSGSFGVQTYELTPNELFGLEIKTRGDGSRFAELVVEKSLDRETQSHYSFRITA LDGGDPPHMGTVGLTIKVTDSNDNNPVFGESTYSVSVPENSPPNTPVIRLNASDPDEGTNGQVVYSFYGY VNDRTRELFQIDPHSGLVTVTGALDYEEGHVYELDVQAKDLGPNSIPAHCKVTVSVLDTNDNPPIINLLS VNSELVEVSESAPPGYVIALVRVSDRDSGLNGRVQCRLLGNVPFRLQEYESFSTILVDGRLDREQHDQYN LTIQARDSGVPMLQSAKSFTVRITDENDNHPHFSKPYYQVIVQENNTPGAYLLSVSARDPDLGLNGSVSY QIVPSQVRDMPVFTYVSINPNSGDIYALRSFNHEQTKAFEFKVLAKDGGMPSLQSNATVRVIILDVNDNT PVITAPPLINGTAEVYIPRNSGIGYLVTVVKADDYDEGENGRVTYDMTEGDRGFFEIDQVNGEVRTTRTF NENSKASYELIVVAHDHGKTSLSASALVLIYLSPALDAQESMGSVNLSLIFIIALGSIAGILFVTMIFVA IKCKRDNKEIRTYNCRIAEYSYGHQKKSSKKKKISKNDIRLVPRDVEETDKMNVVSCSSLTSSLNYFDYH QQTLPLGCRRSESTFLNVENQNTRNTTANHIYHHSFNSQGPQQPDLIINGVPLPETENYSFDSNYVNSRA HLIKSSSTFKDLEGNSLKDSGHEESDQTDSEHDVQRSLYCDTAVNDVLNTSVTSMGSHMPDHDQNDGFHC REECRILGHSDRCWMPRNPMPTRSKSPEHMRNIIALSIEATAADVEAYDDCGPTKRTFATFGKDVSSHPA EERSILKGKRTVDVTICSPKVNSAIREAGNGCEAISPVTSPLHLKSPLPTKPSMSYTVALAPPAHDLDHH ANTGASRPSEAEPRGADSEKVMHEVNPILKEGRDKESPAVKRLKDIIL SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001169129
ORF Size	3294 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001169129.1, NP_001162600.1
RefSeq Size	4156 bp
RefSeq ORF	3297 bp
Locus ID	317183
MW	120.9 kDa

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